A web-based genotyping resource for viral sequences.

abstract：:Analysis of phylogenetically conserved secondary structure has been important in the development of models for the secondary structure of structural RNAs. In this paper, we apply this type of analysis to several families of informational RNAs to evaluate its usefulness in developing secondary structure models for mRNA...

journal_title：Nucleic acids research

authors： Browner MF,Lawrence CB

更新日期：1985-12-09 00:00:00

abstract：:The molecular basis for the DNA binding specificity of the thyroid transcription factor 1 homeodomain (TTF-1HD) has been investigated. Methylation and ethylation interference experiments show that the TTF-1HD alone recapitulates the DNA binding properties of the entire protein. Studies carried out with mutant derivati...

journal_title：Nucleic acids research

authors： Damante G,Fabbro D,Pellizzari L,Civitareale D,Guazzi S,Polycarpou-Schwartz M,Cauci S,Quadrifoglio F,Formisano S,Di Lauro R

更新日期：1994-08-11 00:00:00

abstract：:We analysed the genetic stability of two subtelomeric genes of the human malaria parasite Plasmodium falciparum. A PCR based assay, using a telomere and a target-gene specific primer was used to detect potential chromosome rearrangements. We show that chromosome breakage and the formation of new telomeres occur freque...

journal_title：Nucleic acids research

authors： Scherf A,Mattei D

更新日期：1992-04-11 00:00:00

abstract：:Acetyl-CoA Carboxylase catalyzes the first committed step in fatty acid synthesis. Escherichia coli acetyl-CoA carboxylase is composed of biotin carboxylase, carboxyltransferase and biotin carboxyl carrier protein functions. The accA and accD genes that code for the alpha- and beta-subunits, respectively, are not in a...

journal_title：Nucleic acids research

authors： Meades G Jr,Benson BK,Grove A,Waldrop GL

更新日期：2010-03-01 00:00:00

abstract：:Bgee is a database to retrieve and compare gene expression patterns in multiple animal species, produced by integrating multiple data types (RNA-Seq, Affymetrix, in situ hybridization, and EST data). It is based exclusively on curated healthy wild-type expression data (e.g., no gene knock-out, no treatment, no disease...

journal_title：Nucleic acids research

authors： Bastian FB,Roux J,Niknejad A,Comte A,Fonseca Costa SS,de Farias TM,Moretti S,Parmentier G,de Laval VR,Rosikiewicz M,Wollbrett J,Echchiki A,Escoriza A,Gharib WH,Gonzales-Porta M,Jarosz Y,Laurenczy B,Moret P,Person E,

更新日期：2021-01-08 00:00:00

abstract：:Histone H3K4 methylation is an epigenetic mark associated with actively transcribed genes. This modification is catalyzed by the mixed lineage leukaemia (MLL) family of histone methyltransferases including MLL1, MLL2, MLL3, MLL4, SET1A and SET1B. The catalytic activity of this family is dependent on interactions with ...

journal_title：Nucleic acids research

authors： Kaustov L,Lemak A,Wu H,Faini M,Fan L,Fang X,Zeng H,Duan S,Allali-Hassani A,Li F,Wei Y,Vedadi M,Aebersold R,Wang Y,Houliston S,Arrowsmith CH

更新日期：2019-09-26 00:00:00

abstract：:The hepatocyte nuclear factor (HNF)4alpha, a member of the nuclear receptor superfamily, regulates genes that play a critical role in embryogenesis and metabolism. Recent studies have shown that mutations in the human HNF4alpha gene cause a rare form of type 2 diabetes, maturity onset diabetes of the young (MODY1). To...

journal_title：Nucleic acids research

authors： Lausen J,Thomas H,Lemm I,Bulman M,Borgschulze M,Lingott A,Hattersley AT,Ryffel GU

更新日期：2000-01-15 00:00:00

abstract：:We describe the characterisation of four thermo-stable NAD(+)-dependent DNA ligases, from Thermus thermophilus (Tth), Thermus scotoductus (Ts), Rhodothermus marinus (Rm) and Thermus aquaticus (Taq), by an assay which measures ligation rate and mismatch discrimination. Complete libraries of octa-, nona- and decanucleot...

journal_title：Nucleic acids research

authors： Housby JN,Thorbjarnardóttir SH,Jónsson ZO,Southern EM

更新日期：2000-02-01 00:00:00

abstract：:A DNA fragment d(GCGAAAGCT), known to adopt a stable mini-hairpin structure in solution, has been crystallized in the space group I4(1)22 with the unit-cell dimensions a = b = 53.4 A and c = 54.0 A, and the crystal structure has been determined at 2.5 A resolution. The four nucleotide residues CGAA of the first half o...

journal_title：Nucleic acids research

authors： Sunami T,Kondo J,Kobuna T,Hirao I,Watanabe K,Miura K,Takénaka A

更新日期：2002-12-01 00:00:00

abstract：:Exon arrays are regularly used to analyze differential splicing events. GeneChip Gene 1.0 ST Arrays (gene arrays) manufactured by Affymetrix, Inc. are primarily used to determine expression levels of transcripts, although their basic design is rather similar to GeneChip Exon 1.0 ST Arrays (exon arrays). Here, we show ...

journal_title：Nucleic acids research

authors： Gellert P,Teranishi M,Jenniches K,De Gaspari P,John D,Kreymborg Kg,Braun T,Uchida S

更新日期：2012-03-01 00:00:00

abstract：:The Retinol-Binding Protein (RBP) is expressed primarily in the liver. The regulatory elements involved in its tissue-specific expression have been identified and mapped to the 5' flanking region of the RBP gene. In this paper heterokaryons and somatic cell-hybrids have been produced and analysed in order to demonstra...

journal_title：Nucleic acids research

authors： Faraonio R,Musy M,Colantuoni V

更新日期：1990-12-25 00:00:00

abstract：:Here we describe a method of labelling short oligonucleotide probes with enzyme without purification or chemical modifications. Biotinylated oligonucleotides as short as 10 nt are coupled with streptavidin-conjugated enzyme, hybridised and detected with enzyme-triggered chemiluminescence. The detection of hybridisatio...

journal_title：Nucleic acids research

authors： Guerasimova A,Ivanov I,Lehrach H

更新日期：1999-01-15 00:00:00

abstract：:5' Terminal cap structures of hnRNA have been characterized and the extent of capping determined as a function of embryonic development. Sea urchin embryo hnRNA contains only the type-1 cap, m7GpppNmpNp, with the type-2 cap, which has a 2'-0-methylated subpenultimate nucleotide, being associated only with stable small...

journal_title：Nucleic acids research

authors： Nemer M,Surrey S,Ginzburg I,Echols MM

更新日期：1979-01-01 00:00:00

abstract：:The gene encoding DNA polymerase alpha from the human malaria parasite Plasmodium falciparum has been sequenced and characterised. The deduced amino acid sequence possesses the seven sequence motifs which characterise eukaryotic replicative DNA polymerases (I-VII) and four of five motifs (A-E) identified in alpha DNA ...

journal_title：Nucleic acids research

authors： White JH,Kilbey BJ,de Vries E,Goman M,Alano P,Cheesman S,McAleese S,Ridley RG

更新日期：1993-08-11 00:00:00

abstract：:Developmental stage-specific expression of the β-type globin genes is regulated by many cis- and trans-acting components. The adult β-globin gene contains an E-box located 60 bp downstream of the transcription start site that has been shown to bind transcription factor upstream stimulatory factor (USF) and to contribu...

journal_title：Nucleic acids research

authors： Barrow JJ,Li Y,Hossain M,Huang S,Bungert J

更新日期：2014-04-01 00:00:00

abstract：:Riboswitches are RNA-based regulatory devices that mediate ligand-dependent control of gene expression. However, there has been limited success in rationally designing riboswitches. Moreover, most previous riboswitches are confined to a particular gene and only perform one-way regulation. Here, we used a library scree...

journal_title：Nucleic acids research

authors： Jin Y,Huang JD

更新日期：2011-10-01 00:00:00

abstract：:In small RNA (smRNA) sequencing studies, highly abundant molecules such as adapter dimer products and tissue-specific microRNAs (miRNAs) inhibit accurate quantification of lowly expressed species. We previously developed a method to selectively deplete highly abundant miRNAs. However, this method does not deplete adap...

journal_title：Nucleic acids research

authors： Hardigan AA,Roberts BS,Moore DE,Ramaker RC,Jones AL,Myers RM

更新日期：2019-08-22 00:00:00

abstract：:A 5',7-methylguanosine cap is a quintessential feature of RNA polymerase II-transcribed RNAs, and a textbook aspect of co-transcriptional RNA processing. The cap is bound by the cap-binding complex (CBC), canonically consisting of nuclear cap-binding proteins 1 and 2 (NCBP1/2). Interest in the CBC has recently renewed...

journal_title：Nucleic acids research

authors： Dou Y,Kalmykova S,Pashkova M,Oghbaie M,Jiang H,Molloy KR,Chait BT,Rout MP,Fenyö D,Jensen TH,Altukhov I,LaCava J

更新日期：2020-10-09 00:00:00

abstract：:The opening of DNA double strands is extremely relevant to several biological functions, such as replication and transcription or binding of specific proteins. Such opening phenomenon is particularly sensitive to the aqueous solvent conditions in which the DNA molecule is dispersed, as it can be observed by considerin...

journal_title：Nucleic acids research

authors： Tempestini A,Cassina V,Brogioli D,Ziano R,Erba S,Giovannoni R,Cerrito MG,Salerno D,Mantegazza F

更新日期：2013-02-01 00:00:00

abstract：:The gene, rpb2, encoding the second largest subunit, subunit 2, of RNA polymerase II has been cloned from Schizosaccharomyces pombe using the corresponding gene, RPB2, of Saccharomyces cerevisiae as a probe for cross-hybridization. We have determined the complete nucleotide sequence of rpb2, and parts of the PCR-ampli...

journal_title：Nucleic acids research

authors： Kawagishi M,Yamagishi M,Ishihama A

更新日期：1993-02-11 00:00:00

abstract：:A novel expression system was established that allows expression and propagation of the human hepatitis B virus (HBV) genome in cultured animal cells. An overlength HBV genome encoding the viral pregenomic RNA was put under transcriptional control of the human metallothionein IIA promoter thereby replacing the endogen...

journal_title：Nucleic acids research

authors： Junker M,Galle P,Schaller H

更新日期：1987-12-23 00:00:00

abstract：:Aspartic acid residues comprising the -D-(aa) n -Y-L-D-D- DNA polymerase active site triad of reverse transcriptase from the Saccharomyces cerevisiae long terminal repeat-retrotransposon Ty3 (Asp151, Asp213 and Asp214) were evaluated via site-directed mutagenesis. An Asp151-->Glu substitution showed a dramatic decreas...

journal_title：Nucleic acids research

authors： Bibillo A,Lener D,Klarmann GJ,Le Grice SF

更新日期：2005-01-12 00:00:00

abstract：:Cellular liquid-liquid phase separation (LLPS) results in the formation of dynamic granules that play an important role in many biological processes. On a molecular level, the clustering of proteins into a confined space results from an indefinite network of intermolecular interactions. Here, we introduce and exploit ...

journal_title：Nucleic acids research

authors： Schütz S,Nöldeke ER,Sprangers R

更新日期：2017-06-20 00:00:00

abstract：:Rad54 protein is a key member of the RAD52 epistasis group required for homologous recombination in eukaryotes. Rad54 is a duplex DNA translocase that remodels both DNA and protein-DNA complexes, and functions at multiple steps in the recombination process. Here we use biochemical criteria to demonstrate the existence...

journal_title：Nucleic acids research

authors： Haseltine CA,Kowalczykowski SC

更新日期：2009-05-01 00:00:00

abstract：:The nucleotide sequence of 5.8S rRNA from the Chinese silkworm Philosamia cynthia ricini has been determined by gel sequencing and mobility shift methods. The complete primary structure is (sequence in text). This is one of the largest known 5.8S rRNAs. As compared to Bombyx 5.8S rRNA, it is two nucleotides longer; t...

journal_title：Nucleic acids research

authors： Feng YX,Krupp G,Gross HJ

更新日期：1982-10-25 00:00:00

abstract：:TFIIIC is a RNA polymerase (pol) III-specific DNA-binding factor that is required for transcription of tRNA and 5S rRNA genes. Active human TFIIIC consists of five subunits. However, an inactive form has also been isolated that lacks one of the five subunits, called TFIIIC110. A model was proposed in which pol III tra...

journal_title：Nucleic acids research

authors： Innes F,Ramsbottom B,White RJ

更新日期：2006-07-05 00:00:00

abstract：:A cDNA clone covering the whole coding region for a glycinin subunit precursor containing the A1a acidic subunit, one of the A2 family, has been identified from a library of soybean cotyledonary cDNA clones using a mixed oligonucleotide probe. Analysis of the cDNA insert revealed that it contained 1746 nucleotides of ...

journal_title：Nucleic acids research

authors： Negoro T,Momma T,Fukazawa C

更新日期：1985-09-25 00:00:00

abstract：:Mild ultraviolet irradiation of E. coli ribosomal subunits leads to the formation of a number of intra-RNA cross-links, in addition to the RNA-protein cross-links already reported (see refs. 9, 10). After partial ribonuclease digestion of the RNA from irradiated subunits, complexes containing these intra-RNA cross-lin...

journal_title：Nucleic acids research

authors： Zwieb C,Brimacombe R

更新日期：1980-06-11 00:00:00

abstract：:The complete nucleotide sequence of the mitochondrial DNA of the amphioxus Branchiostoma lanceolatum has been determined. This mitochondrial genome is small (15 076 bp) because of the short size of the two rRNA genes and the tRNA genes. In addition, this genome contains a very short non-coding region (57 bp) with no s...

journal_title：Nucleic acids research

authors： Spruyt N,Delarbre C,Gachelin G,Laudet V

更新日期：1998-07-01 00:00:00

abstract：:FANTOM DB, the database of Functional Annotation of RIKEN Mouse cDNA Clones, is designed to store sequence information of RIKEN full-length enriched mouse cDNA clones, graphical views of sequence analysis results, curated functional annotation information and additional descriptions, including Gene Ontology terms. RIK...

journal_title：Nucleic acids research

authors： Bono H,Kasukawa T,Furuno M,Hayashizaki Y,Okazaki Y

更新日期：2002-01-01 00:00:00