Abstract:
:A Fortran computer algorithm has been used to analyze the nucleotide sequence of several structural genes. The analysis performed on both coding and complementary DNA strands shows that whereas open reading frames shorter than 100 codons are randomly distributed on both DNA strands, open reading frames longer than 100 codons ("virtual genes") are significantly more frequent on the complementary DNA strand than on the coding one. These "virtual genes" were further investigated by looking at intron sequences, splicing points, signal sequences and by analyzing gene mutations. On the basis of this analysis coding and complementary DNA strands of several eukaryotic structural genes cannot be distinguished. In particular we suggest that the complementary DNA strand of the human epsilon-globin gene might indeed code for a protein.
journal_name
Nucleic Acids Resjournal_title
Nucleic acids researchauthors
Casino A,Cipollaro M,Guerrini AM,Mastrocinque G,Spena A,Scarlato Vdoi
10.1093/nar/9.6.1499subject
Has Abstractpub_date
1981-03-25 00:00:00pages
1499-518issue
6eissn
0305-1048issn
1362-4962journal_volume
9pub_type
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