Abstract:
:We developed a sensitive 32P-postlabeling method for the detection of bifunctional intrastrand crosslinks d(Pt-GpG) and d(Pt-ApG) in DNA in vitro and in vivo. After enzymatic digestion of DNA the positively charged platinum adducts were purified from unplatinated products, using strong cation exchange chromatography. Subsequently the samples were deplatinated with cyanide, because platinated dinucleotides are very poor substrates for polynucleotide kinase. The excess of cyanide was removed using Sep-pak C18 cartridges, and the resulting dinucleoside monophosphates d(GpG) and d(ApG) were subsequently postlabelled. Analysis of the postlabelling mixture was performed by a combined TLC and HPLC-procedure. Good correlations with existing methods (AAS, immunocytochemistry and ELISA) were found in DNA samples treated in vitro and in vivo with cis- or carboplatin. The detection limit of the assay was 1 adduct/10(7) nucleotides in a 10 micrograms DNA sample.
journal_name
Nucleic Acids Resjournal_title
Nucleic acids researchauthors
Blommaert FA,Saris CPdoi
10.1093/nar/23.8.1300subject
Has Abstractpub_date
1995-04-25 00:00:00pages
1300-6issue
8eissn
0305-1048issn
1362-4962pii
5b0016journal_volume
23pub_type
杂志文章abstract::The RecQ family of helicases is highly conserved both structurally and functionally from bacteria to humans. Defects in human RecQ helicases are associated with genetic diseases that are characterized by cancer predisposition and/or premature aging. RecQ proteins exhibit 3'-5' helicase activity and play critical roles...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkz708
更新日期:2019-10-10 00:00:00
abstract::Isogenic pluripotent stem cells are critical tools for studying human neurological diseases by allowing one to study the effects of a mutation in a fixed genetic background. Of particular interest are the spectrum of autism disorders, some of which are monogenic such as Timothy syndrome (TS); others are multigenic suc...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkv164
更新日期:2015-05-26 00:00:00
abstract::We describe here an energy based computer software suite for narrowing down the search space of tertiary structures of small globular proteins. The protocol comprises eight different computational modules that form an automated pipeline. It combines physics based potentials with biophysical filters to arrive at 10 pla...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkl789
更新日期:2006-01-01 00:00:00
abstract::In order to facilitate the direct computation of the sizes of DNA fragments separated by gel electrophoresis, we have written and evaluated programmes for the Hewlett-Packard 41C programmable calculator. The sizes estimated for DNA fragments of known length using some of these programmes were found to be more accurate...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/12.1part2.845
更新日期:1984-01-11 00:00:00
abstract::GenBank is a comprehensive database that contains publicly available DNA sequences for more than 140 000 named organisms, obtained primarily through submissions from individual laboratories and batch submissions from large-scale sequencing projects. Most submissions are made using the BankIt (web) or Sequin program an...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkh045
更新日期:2004-01-01 00:00:00
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journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/18.5.1145
更新日期:1990-03-11 00:00:00
abstract::Right-handed RNA duplexes of (CG)n sequence undergo salt-induced helicity reversal, forming left-handed RNA double helices (Z-RNA). In contrast to the thoroughly studied Z-DNA, no Z-RNA structure of natural origin is known. Here we report the NMR structure of a half-turn, left-handed RNA helix (CGCGCG)2 determined in ...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkh736
更新日期:2004-08-03 00:00:00
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journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gky386
更新日期:2018-06-20 00:00:00
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journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkaa220
更新日期:2020-07-02 00:00:00
abstract::FANTOM DB, the database of Functional Annotation of RIKEN Mouse cDNA Clones, is designed to store sequence information of RIKEN full-length enriched mouse cDNA clones, graphical views of sequence analysis results, curated functional annotation information and additional descriptions, including Gene Ontology terms. RIK...
journal_title:Nucleic acids research
pub_type: 杂志文章
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更新日期:2002-01-01 00:00:00
abstract::Stable protein complexes, including those formed with RNA, are major building blocks of every living cell. Escherichia coli has been the leading bacterial organism with respect to global protein-protein networks. Yet, there has been no global census of RNA/protein complexes in this model species of microbiology. Here,...
journal_title:Nucleic acids research
pub_type: 杂志文章
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更新日期:2020-09-18 00:00:00
abstract::High-throughput sequencing technologies enable direct approaches to catalog and analyze snapshots of the total small RNA content of living cells. Characterization of high-throughput sequencing data requires bioinformatic tools offering a wide perspective of the small RNA transcriptome. Here we present SeqBuster, a hig...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkp1127
更新日期:2010-03-01 00:00:00
abstract::The double helix is known to form as a result of hybridization of complementary nucleic acid strands in aqueous solution. In the helix the negatively charged phosphate groups of each nucleic acid strand are distributed helically on the outside of the duplex and are available for interaction with cationic groups. Catio...
journal_title:Nucleic acids research
pub_type: 杂志文章
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更新日期:2001-07-15 00:00:00
abstract::Treatment of responsive cells by interferons (IFNs) induces within a few hours a rise in the concentration of several proteins and mRNAs. In order to characterize these IFN-induced mRNA species, we have cloned in E. coli the cDNA made from a 17-18S poly(A)+ RNA of human fibroblastoid cells (SV80) treated with IFN-beta...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/11.5.1213
更新日期:1983-03-11 00:00:00
abstract::Rfam is a comprehensive collection of non-coding RNA (ncRNA) families, represented by multiple sequence alignments and profile stochastic context-free grammars. Rfam aims to facilitate the identification and classification of new members of known sequence families, and distributes annotation of ncRNAs in over 200 comp...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gki081
更新日期:2005-01-01 00:00:00
abstract::Eukaryotes produce multiple products from a single gene locus by alternative splicing, translation or promoter usage as mechanisms expanding the complexity of their proteome. Trithorax proteins, including the Arabidopsis Trithorax-like protein ATX1, are histone modifiers regulating gene activity. Here, we report that ...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkq1300
更新日期:2011-06-01 00:00:00
abstract::Ribonucleic acids are highly conserved essential parts of cellular life. RNA function is determined to a large extent by its hydrodynamic behaviour. The presented study proposes a strategy to predict the hydrodynamic behaviour of RNA single strands on the basis of the polymer size. By atom-level shell-modelling of hig...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkq808
更新日期:2011-02-01 00:00:00
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journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkz1081
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journal_title:Nucleic acids research
pub_type: 杂志文章
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更新日期:2005-07-01 00:00:00
abstract::The role of TATA elements in the expression of a mammalian promoter was investigated in the fission yeast Schizosaccharomyces pombe, by studying the human adenovirus E2-early promoter. This is a unique dual promoter with two nonconsensus TATA elements directing transcription from two cap sites, +1 and -26. A sequence ...
journal_title:Nucleic acids research
pub_type: 杂志文章
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更新日期:1993-06-11 00:00:00
abstract::Two DNA polymerases have been purified from the 105,000 x g supernatant of ungerminated wheat. The purification stages included: high speed centrifugation, salt fractionation, DEAE-cellulose chromatography, Sephadex G-150 filtration and phosphocellulose chromatography. Several properties of the two enzyme (called A an...
journal_title:Nucleic acids research
pub_type: 杂志文章
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更新日期:1975-11-01 00:00:00
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journal_title:Nucleic acids research
pub_type: 杂志文章
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更新日期:2002-02-15 00:00:00
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journal_title:Nucleic acids research
pub_type: 杂志文章
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更新日期:1991-02-11 00:00:00
abstract::We have isolated and sequenced a cDNA clone coding for human Retinol Binding Protein. The sequence indicates that Retinol Binding Protein is synthesized as a single polypeptide chain precursor which is then matured to the secreted protein by removal of a leader peptide. Southern and Northern blot analysis suggest that...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/11.22.7769
更新日期:1983-11-25 00:00:00
abstract::The binding of anti poly(I). poly (C) Fab fragments to double or triple stranded polynucletides has been studied by fluorescence. Association constants were deduced from competition experiments. The comparison of the association constants leads to the conclusion that several atoms of the base residues do not interact ...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/3.12.3337
更新日期:1976-12-01 00:00:00
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journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/12.17.6647
更新日期:1984-09-11 00:00:00
abstract::The high-throughput sequencing of nuclease-protected mRNA fragments bound to ribosomes, a technique known as ribosome profiling, quantifies the relative frequencies with which different regions of transcripts are translated. This technique has revealed novel translation initiation sites with unprecedented scope and ha...
journal_title:Nucleic acids research
pub_type: 杂志文章
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更新日期:2015-04-20 00:00:00
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journal_title:Nucleic acids research
pub_type: 杂志文章
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更新日期:2009-06-01 00:00:00
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journal_title:Nucleic acids research
pub_type: 杂志文章
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更新日期:2020-07-02 00:00:00
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journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/23.7.1113
更新日期:1995-04-11 00:00:00