Abstract:
:Treatment of responsive cells by interferons (IFNs) induces within a few hours a rise in the concentration of several proteins and mRNAs. In order to characterize these IFN-induced mRNA species, we have cloned in E. coli the cDNA made from a 17-18S poly(A)+ RNA of human fibroblastoid cells (SV80) treated with IFN-beta. We describe here a pBR322 recombinant plasmid (C56) which contains a 400 bp cDNA insert corresponding to a 18S mRNA species newly induced by IFN. The C56 mRNA codes for a 56,000 dalton protein easily detectable by hybridization-translation experiments. The sequence of 66 of the carboxy-terminal amino-acids of the protein can be deduced from the cDNA sequence. IFNs-alpha, beta or gamma are able to activate the expression of this gene in human fibroblasts as well as lymphoblastoid cells. The mRNA is not detectable without IFN; it reaches maximum levels (0.1% of the total poly(A)+ RNA) within 4-8 hrs and decreases after 16 hrs.
journal_name
Nucleic Acids Resjournal_title
Nucleic acids researchauthors
Chebath J,Merlin G,Metz R,Benech P,Revel Mdoi
10.1093/nar/11.5.1213subject
Has Abstractpub_date
1983-03-11 00:00:00pages
1213-26issue
5eissn
0305-1048issn
1362-4962journal_volume
11pub_type
杂志文章abstract::The strand displacement activity of DNA polymerase δ is strongly stimulated by its interaction with proliferating cell nuclear antigen (PCNA). However, inactivation of the 3'-5' exonuclease activity is sufficient to allow the polymerase to carry out strand displacement even in the absence of PCNA. We have examined in ...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkv260
更新日期:2015-04-30 00:00:00
abstract::Within a single infected individual, a virus population can have a high genomic variability. In the case of HIV, several mutations can be present even in a small genomic window of 20-30 nucleotides. For diagnostics purposes, it is often needed to resequence genomic subsets where crucial mutations are known to occur. I...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkt682
更新日期:2013-10-01 00:00:00
abstract::We described here an approach for mapping proteins on any sequence of genomic DNA. UV-induced DNA-protein crosslinking within whole cells and the 'protein image' hybridization technique (1) were applied to test the proteins bound to different regions of the D. melanogaster hsp-70 gene. The histone H1-DNA association w...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/21.4.1031
更新日期:1993-02-25 00:00:00
abstract::The gene encoding the DNA methyltransferase M.CviRI from Chlorella virus XZ-6E was cloned and expressed in Escherichia coli. M.CviRI methylates adenine in TGCA sequences. DNA containing the M.CviRI gene was sequenced and a single open reading frame of 1137 bp was identified which could code for a polypeptide of 379 am...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/19.2.307
更新日期:1991-01-25 00:00:00
abstract::Chicken erythrocyte inner histones (H2A, H2B, H3 and H4) were associated with the two complementary homopolymeric polydeoxyribonucleotides and the two alternating copolymeric polydeoxyribonucleotides. No evidence for formation of chromatin-like structures was obtained for the complexes with poly(dG) . poly(dC) or poly...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/6.4.1387
更新日期:1979-04-01 00:00:00
abstract::Hammerhead ribozymes were transcribed from a dsDNA template containing four random nucleotides between stems II and III, which replace the naturally occurring GAA nucleotides. In vitro selection was used to select hammerhead ribozymes capable of in cis cleavage using denaturing polyacrylamide gels for the isolation of...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/24.22.4401
更新日期:1996-11-15 00:00:00
abstract::We have previously reported the isolation of mammalian cell lines expressing the 3-methyladenine DNA glycosylase I (tag) gene from E. coli. These cells are 2-5 fold more resistant to the toxic effects of methylating agents than normal cells (15). Kinetic measurements of 3-methyladenine removal from the genome in situ ...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/22.9.1670
更新日期:1994-05-11 00:00:00
abstract::The molecular mechanisms that underlie the neurological manifestations of patients with inherited diseases of vitamin B12 (cobalamin) metabolism remain to date obscure. We observed transcriptomic changes of genes involved in RNA metabolism and endoplasmic reticulum stress in a neuronal cell model with impaired cobalam...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gky634
更新日期:2018-09-06 00:00:00
abstract::The Protein Data Bank in Europe (PDBe, pdbe.org) is actively engaged in the deposition, annotation, remediation, enrichment and dissemination of macromolecular structure data. This paper describes new developments and improvements at PDBe addressing three challenging areas: data enrichment, data dissemination and func...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkx1070
更新日期:2018-01-04 00:00:00
abstract::SSCP and heteroduplex analysis (HA) continue to be the most popular methods of mutation detection due to their simplicity, high sensitivity and low cost. The advantages of these methods are most clearly visible when large genes, such as BRCA1 and BRCA2, are scanned for scattered unknown mutations and/or when a large n...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/29.14.e71
更新日期:2001-07-15 00:00:00
abstract::Heterochromatin protein 1 (HP1) is an evolutionarily conserved chromosomal protein that binds to lysine 9-methylated histone H3 (H3K9me), a hallmark of heterochromatin. Although HP1 phosphorylation has been described in several organisms, the biological implications of this modification remain largely elusive. Here we...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gku995
更新日期:2014-11-10 00:00:00
abstract::Cortical development is controlled by transcriptional programs, which are orchestrated by transcription factors. Yet, stable inheritance of spatio-temporal activity of factors influencing cell fate and localization in different layers is only partly understood. Here we find that deletion of Dot1l in the murine telence...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gky953
更新日期:2019-01-10 00:00:00
abstract::(19)F NMR spectroscopy has proved to be a valuable tool to monitor functionally important conformational transitions of nucleic acids. Here, we present a systematic investigation on the application of 5-fluoro pyrimidines to probe DNA and RNA secondary structures. Oligonucleotides with the propensity to adapt secondar...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkp862
更新日期:2009-12-01 00:00:00
abstract::We report the isolation of a novel human POU domain encoding gene named RDC-1. The POU domain of the RDC-1 encoded protein is highly related to the POU domain potentially encoded by the rat brain-3 sequence and to that of the Drosophila I-POU protein; outside of the POU region, RDC-1 is unrelated to any previously cha...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/20.18.4919
更新日期:1992-09-25 00:00:00
abstract::Assembly factors provide speed and directionality to the maturation process of the 30S subunit in bacteria. To gain a more precise understanding of how these proteins mediate 30S maturation, it is important to expand on studies of 30S assembly intermediates purified from bacterial strains lacking particular maturation...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkz571
更新日期:2019-09-05 00:00:00
abstract::The STE2 gene of the yeast Saccharomyces cerevisiae encodes a component of the receptor for the oligopeptide pheromone alpha-factor. We have cloned and determined the nucleotide sequence of the STE2 gene. A sequence involved in the control of cell-type expression of the STE2 gene was found 5' of an open reading frame ...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/13.23.8463
更新日期:1985-12-09 00:00:00
abstract::Mutations in human mitochondrial DNA are often associated with incurable human neuromuscular diseases. Among these mutations, an important number have been identified in tRNA genes, including 29 in the gene MT-TL1 coding for the tRNA(Leu(UUR)). The m.3243A>G mutation was described as the major cause of the MELAS syndr...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkr546
更新日期:2011-10-01 00:00:00
abstract::Leishmania species are protozoan parasites whose remarkably plastic genome limits the establishment of effective genetic manipulation and leishmaniasis treatment. The strategies used by Leishmania to maintain its genome while allowing variability are not fully understood. Here, we used DiCre-mediated conditional gene ...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gky1017
更新日期:2018-12-14 00:00:00
abstract::The generalized mismatch repair system of Streptococcus pneumoniae (the Hex system) can eliminate base pair mismatches arising in heteroduplex DNA during transformation or by DNA polymerase errors during replication. Mismatch repair is most likely initiated at nicks or gaps. The present work was started to examine the...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/19.20.5525
更新日期:1991-10-25 00:00:00
abstract::A formaldehyde denaturation map of the replicative form of phiX174 DNA is obtained. The RFI DNA was converted into a linear state by restriction endonuclease pst I which introduces into this DNA a single double-stranded break. The map has four clear-cut peaks. Their positions excellently correlate with the peak positi...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/5.7.2493
更新日期:1978-07-01 00:00:00
abstract::The C (pifC) protein of miniF represses transcription of its own gene by binding to the pif operator (pifO); it is also needed for replication initiated from the miniF primary origin (ori-1). We have determined the nucleotide sequence of the C gene. The gene has been inserted into an expression vector under Ptrp contr...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/14.24.9699
更新日期:1986-12-22 00:00:00
abstract::RNA co-transcriptional folding has long been suspected to play an active role in helping proper native folding of ribozymes and structured regulatory motifs in mRNA untranslated regions (UTRs). Yet, the underlying mechanisms and coding requirements for efficient co-transcriptional folding remain unclear. Traditional a...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkl1036
更新日期:2007-01-01 00:00:00
abstract::The complete nucleotide sequences of the various precursor 5S RNA species occurring in Bacillus licheniformis have been elucidated. The B. licheniformis precursors contain a 5'-precursor-specific segment of 95 nucleotides which is four times as long as the corresponding segment of the p5S RNAs from the closely related...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/8.19.4535
更新日期:1980-10-10 00:00:00
abstract::Because of their high nucleotide sequence homology, the specific detection of mRNA transcripts of individual members of a gene family presents certain problems. Here we apply and defend the single nucleotide primer extension (SNuPE) as a simple, specific, and highly quantitative assay for this purpose. The method dist...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/24.23.4812
更新日期:1996-12-01 00:00:00
abstract::We describe here the cloning, characterization and expression in E. coli of the gene coding for a DNA methylase from Spiroplasma sp. strain MQ1 (M.SssI). This enzyme methylates completely and exclusively CpG sequences. The Spiroplasma gene was transcribed in E. coli using its own promoter. Translation of the entire me...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/18.5.1145
更新日期:1990-03-11 00:00:00
abstract::A downstream G-rich sequence (GRS), GGGGGAGGUGUGGG, has been previously shown to influence the efficiency of 3' end processing of the SV40 late polyadenylation signal. We have now defined several important parameters for GRS-mediated polyadenylation. The ability of the GRS to influence 3' end processing efficiency was...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/23.9.1625
更新日期:1995-05-11 00:00:00
abstract::The discoidin proteins of Dictyostelium discoideum are highly expressed during development. The Disc I gamma promoter allows the regulation of heterologous protein expression by experimental conditions. We report conditions under which the promoter activity is efficiently repressed during growth in the wildtype strain...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/21.6.1397
更新日期:1993-03-25 00:00:00
abstract::SNiPlay is a web-based tool for detection, management and analysis of genetic variants including both single nucleotide polymorphisms (SNPs) and InDels. Version 3 now extends functionalities in order to easily manage and exploit SNPs derived from next generation sequencing technologies, such as GBS (genotyping by sequ...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkv351
更新日期:2015-07-01 00:00:00
abstract::Peptidome similarity analysis enables researchers to gain insights into differential peptide profiles, providing a robust tool to discriminate strain-specific peptides, true intra-species differences among biological replicates or even microorganism-phenotype variations. However, no in silico peptide fingerprinting so...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkx389
更新日期:2017-07-03 00:00:00
abstract::The RSC complex of Saccharomyces cerevisiae is closely related to the SWI/SNF complex. Both complexes are involved in remodeling chromatin structure and they share conserved components. The RSC proteins Sth1, Rsc8/Swh3, Sfh1 and Rsc6 are homologs of the SWI/SNF proteins Swi2/Snf2, Swi3, Snf5 and Swp73 respectively. To...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/26.16.3739
更新日期:1998-08-15 00:00:00