Genetic manipulation of an exogenous non-immunoglobulin protein by gene conversion machinery in a chicken B cell line.

abstract：:The GenBank((R))sequence database incorporates publicly available DNA sequences of >55 000 different organisms, primarily through direct submission of sequence data from individual laboratories and large-scale sequencing projects. Most submissions are made using the BankIt (Web) or Sequin programs and accession number...

journal_title：Nucleic acids research

authors： Benson DA,Karsch-Mizrachi I,Lipman DJ,Ostell J,Rapp BA,Wheeler DL

更新日期：2000-01-01 00:00:00

abstract：:Insertion of DNA containing PR, the early rightward promoter of bacteriophage lambda, is lethal to M13-derived vectors when the promoter directs transcription (using the '+' strand as template) toward the M13 origin of replication (ori). Lethality can be relieved by mutation of PR, repression of the promoter by the la...

journal_title：Nucleic acids research

authors： Brown S,Ferm J,Woody S,Gussin G

更新日期：1990-10-25 00:00:00

abstract：:E2F transcription factors (E2F1-8) are known to coordinately regulate the expression of a plethora of target genes, including those coding for microRNAs (miRNAs), to control cell cycle progression. Recent work has described the atypical E2F factor E2F7 as a transcriptional repressor of cell cycle-related protein-codin...

journal_title：Nucleic acids research

authors： Mitxelena J,Apraiz A,Vallejo-Rodríguez J,Malumbres M,Zubiaga AM

更新日期：2016-07-08 00:00:00

abstract：:The Sleeping Beauty (SB) transposon is a promising gene transfer vector that integrates nonspecifically into host cell genomes. Herein, we attempt to direct transposon integration into predetermined DNA sites by coupling a site-specific DNA-binding domain (DBD) to the SB transposase. We engineered fusion proteins comp...

journal_title：Nucleic acids research

authors： Yant SR,Huang Y,Akache B,Kay MA

更新日期：2007-01-01 00:00:00

abstract：:Hippo pathway is involved in tumorigenesis, and its regulation in cytosol has been extensively studied, but its regulatory mechanisms in the nuclear are not clear. In the current study, using a FBS-inducing model following serum starvation, we identified KDM3A, a demethylase of histone H3K9me1/2, as a positive regulat...

journal_title：Nucleic acids research

authors： Wang HY,Long QY,Tang SB,Xiao Q,Gao C,Zhao QY,Li QL,Ye M,Zhang L,Li LY,Wu M

更新日期：2019-03-18 00:00:00

abstract：:Knowledge of the various interactions between molecules in the cell is crucial for understanding cellular processes in health and disease. Currently available interaction databases, being largely complementary to each other, must be integrated to obtain a comprehensive global map of the different types of interactions...

journal_title：Nucleic acids research

authors： Kamburov A,Stelzl U,Lehrach H,Herwig R

更新日期：2013-01-01 00:00:00

abstract：:Transcription initiation is a major step in gene regulation for all organisms. In bacteria, the promoter DNA is first recognized by RNA polymerase (RNAP) to yield an initial closed complex. This complex subsequently undergoes conformational changes resulting in DNA strand separation to form a transcription bubble and ...

journal_title：Nucleic acids research

authors： Duchi D,Gryte K,Robb NC,Morichaud Z,Sheppard C,Brodolin K,Wigneshweraraj S,Kapanidis AN

更新日期：2018-01-25 00:00:00

abstract：:Given that greater than 90% of the human genome is expressed, it is logical to assume that post-transcriptional regulatory mechanisms must be the primary means of controlling the flow of information from mRNA to protein. This report describes a robust approach that includes in silico, in vitro and in cellulo experimen...

journal_title：Nucleic acids research

authors： Beaudoin JD,Perreault JP

更新日期：2010-11-01 00:00:00

abstract：:The Kluyveromyces lactis gene encoding the general transcription factor IIB (TFIIB) was isolated from a genomic library by complementation of the cold-sensitive phenotype conferred by a mutation in the SUA7 gene, which encodes TFIIB in Saccharomyces cerevisiae. DNA sequence analysis of the KI-SUA7 gene revealed a 357 ...

journal_title：Nucleic acids research

authors： Na JG,Hampsey M

更新日期：1993-07-25 00:00:00

abstract：:The heterogeneous nuclear ribonucleoprotein (hnRNP) A2 is a multi-tasking protein that acts in the cytoplasm and nucleus. We have explored the possibility that this protein is associated with telomeres and participates in their maintenance. Rat brain hnRNP A2 was shown to have two nucleic acid binding sites. In the pr...

journal_title：Nucleic acids research

authors： Moran-Jones K,Wayman L,Kennedy DD,Reddel RR,Sara S,Snee MJ,Smith R

更新日期：2005-01-19 00:00:00

abstract：:Interferons (IFN) play a pivotal role in innate immunity, orchestrating a cell-intrinsic anti-pathogenic state and stimulating adaptive immune responses. The complex interplay between the primary response to IFNs and its modulation by positive and negative feedback loops is incompletely understood. Here, we implement ...

journal_title：Nucleic acids research

authors： Trilling M,Bellora N,Rutkowski AJ,de Graaf M,Dickinson P,Robertson K,Prazeres da Costa O,Ghazal P,Friedel CC,Albà MM,Dölken L

更新日期：2013-09-01 00:00:00

abstract：:An oligodeoxyribonucleotide, d(GCTCACAAT-X-ATTGTGAGC), where X represents a hexaethylene glycol chain, was studied using circular dichroism spectroscopy. Its conformation and conformational stability were compared to those of compounds where X was replaced by four thymines and to the duplex of same sequence without lo...

journal_title：Nucleic acids research

authors： Durand M,Chevrie K,Chassignol M,Thuong NT,Maurizot JC

更新日期：1990-11-11 00:00:00

abstract：:Three Rat1/Xrn2 homologues exist in Arabidopsis thaliana: nuclear AtXRN2 and AtXRN3, and cytoplasmic AtXRN4. The latter has a role in degrading 3' products of miRNA-mediated mRNA cleavage, whereas all three proteins act as endogenous post-transcriptional gene silencing suppressors. Here we show that, similar to yeast ...

journal_title：Nucleic acids research

authors： Zakrzewska-Placzek M,Souret FF,Sobczyk GJ,Green PJ,Kufel J

更新日期：2010-07-01 00:00:00

abstract：:We have sequenced the entire human S mu and S gamma 4 immunoglobulin heavy chain class switch regions, and have also completed the sequence of human S epsilon. S mu is composed predominantly of GAGCT and GGGCT pentameric repeats, with these units also being found in S epsilon at a much lower density. S mu-S gamma 4 ma...

journal_title：Nucleic acids research

authors： Mills FC,Brooker JS,Camerini-Otero RD

更新日期：1990-12-25 00:00:00

abstract：:Generation of double-stranded cDNA during reverse transcription of a variety of mRNA molecules is well known to involve the formation of covalently linked antisense and sense strands in a hairpin configuration. In the present study we have examined the sequence of molecular events which occurs during cDNA synthesis fr...

journal_title：Nucleic acids research

authors： Volloch VZ,Schweitzer B,Rits S

更新日期：1994-12-11 00:00:00

abstract：:In non-malignant RWPE-1 prostate epithelial cells signaling by the nuclear receptor Vitamin D Receptor (VDR, NR1I1) induces cell cycle arrest through targets including CDKN1A (encodes p21((waf1/cip1))). VDR dynamically induced individual histone modification patterns at three VDR binding sites (R1, 2, 3) on the CDKN1A...

journal_title：Nucleic acids research

authors： Thorne JL,Maguire O,Doig CL,Battaglia S,Fehr L,Sucheston LE,Heinaniemi M,O'Neill LP,McCabe CJ,Turner BM,Carlberg C,Campbell MJ

更新日期：2011-03-01 00:00:00

abstract：:Ethidium causes the cleavage of DNA via a light and oxygen dependent process. Using covalently closed circular DNA as a substrate, the saturation kinetics and the dependence on superhelical density of the cleavage indicate that intercalated ethidium is mainly responsible for nicking DNA. Superoxide dismutase has littl...

journal_title：Nucleic acids research

authors： Deniss IS,Morgan AR

更新日期：1976-02-01 00:00:00

abstract：:We describe a dedicated RNA motif search program and web server to identify RNA riboswitches. The Riboswitch finder analyses a given sequence using the web interface, checks specific sequence elements and secondary structure, calculates and displays the energy folding of the RNA structure and runs a number of tests in...

journal_title：Nucleic acids research

authors： Bengert P,Dandekar T

更新日期：2004-07-01 00:00:00

abstract：:In 4M NaCl solutions (dC-dG)n (n = 3,4; approximately 9 mM) exist as a mixture o +/- B and Z forms. The low and high field components of two 31P NMR resonances originating from internal phosphodiester groups are assigned to the GpC and CpG linkages, respectively. Low temperatures stabilize the Z-forms, which completel...

journal_title：Nucleic acids research

authors： Holak TA,Borer PN,Levy GC,van Boom JH,Wang AH

更新日期：1984-06-11 00:00:00

abstract：:Mono- and polyclonal antibodies directed against different domains of the potato leafroll luteovirus (PLRV) P1 (ORF1) protein were applied to the analysis of P1 expression during PLRV replication in planta. Western analyses detected P1 and a protein of approximately 25 kDa (P1-C25) that accumulated to readily detectab...

journal_title：Nucleic acids research

authors： Prüfer D,Kawchuk L,Monecke2 M,Nowok S,Fischer R,Rohde W

更新日期：1999-01-15 00:00:00

abstract：:Human minisatellite probes cross-hybridize to mouse DNA and detect multiple variable loci. The resulting DNA "fingerprints" vary substantially between inbred strains but relatively little within an inbred strain. By studying the segregation of variable DNA fragments in BXD recombinant inbred strains of mice, at least ...

journal_title：Nucleic acids research

authors： Jeffreys AJ,Wilson V,Kelly R,Taylor BA,Bulfield G

更新日期：1987-04-10 00:00:00

abstract：:Genomes mutate and evolve in ways simple (substitution or deletion of bases) and complex (e.g. chromosome shattering). We do not fully understand what types of complex mutation occur, and we cannot routinely characterize arbitrarily-complex mutations in a high-throughput, genome-wide manner. Long-read DNA sequencing m...

journal_title：Nucleic acids research

authors： Frith MC,Khan S

更新日期：2018-02-28 00:00:00

abstract：:Nucleosomes released from oviduct nuclei during brief micrococcal nuclease digestions are enriched in transcribed sequences (bloom K.S. and Anderson, J.N. (1978) Cell, 15, 141-150). Such nucleosomes released into this 1Sf supernatant fraction are enriched in proteins HMG14, 17 and a third lower molecular weight protei...

journal_title：Nucleic acids research

authors： Goodwin GH,Wright CA,Johns EW

更新日期：1981-06-25 00:00:00

abstract：:Cloned ribosomal DNA (rDNA) of Locusta migratoria was analyzed by restriction site mapping and SI nuclease experiments. The repeat unit is 18 kb long. The nontranscribed spacer region (NTS) is very large (11 kb) and homogeneous in length, but many of the restriction sites are heterogeneous among the repeat units. Two ...

journal_title：Nucleic acids research

authors： Schäfer M,Kunz W

更新日期：1985-02-25 00:00:00

abstract：:Yaf9 is an integral part of the NuA4 acetyltransferase and the SWR1 chromatin remodeling complexes. Here, we show that Yaf9 associates with acetylated histone H3 with high preference for H3K27ac. The crystal structure of the Yaf9 YEATS domain bound to the H3K27ac peptide reveals that the sequence C-terminal to K27ac s...

journal_title：Nucleic acids research

authors： Klein BJ,Ahmad S,Vann KR,Andrews FH,Mayo ZA,Bourriquen G,Bridgers JB,Zhang J,Strahl BD,Côté J,Kutateladze TG

更新日期：2018-01-09 00:00:00

abstract：:A mass culture of human diploid fibroblasts, and eight clones isolated from that mass culture, were examined for methylation patterns in several regions of DNA. Plasmid-inserted cDNA sequences were used as probes for alpha-hCG, beta-globin, A gamma- and G gamma-globin, and beta- and gamma-actin gene regions. Each prob...

journal_title：Nucleic acids research

authors： Shmookler Reis RJ,Goldstein S

更新日期：1982-07-24 00:00:00

abstract：:MDg3 is a family of mobile dispersed genetic elements represented by 15 copies in the haploid genome of D. melanogaster and flanked, like other similar elements, by the regions of homology. In the present work, these regions of mdg3 have been sequenced. The existence of perfect direct repeats 268 base pairs long has b...

journal_title：Nucleic acids research

authors： Bayev AA Jr,Krayev AS,Lyubomirskaya NV,Ilyin YV,Skryabin KG,Georgiev GP

更新日期：1980-08-11 00:00:00

abstract：:As large-scale re-sequencing of genomes reveals many protein mutations, especially in human cancer tissues, prediction of their likely functional impact becomes important practical goal. Here, we introduce a new functional impact score (FIS) for amino acid residue changes using evolutionary conservation patterns. The ...

journal_title：Nucleic acids research

authors： Reva B,Antipin Y,Sander C

更新日期：2011-09-01 00:00:00

abstract：:We have developed an effective, easy-to-use two-step system for the site-directed insertion of large genetic constructs into arbitrary positions in the Escherichia coli chromosome. The system uses lambda-Red mediated recombineering accompanied by the introduction of double-strand DNA breaks in the chromosome and a don...

journal_title：Nucleic acids research

authors： Kuhlman TE,Cox EC

更新日期：2010-04-01 00:00:00

abstract：:Many interacting proteins regulate and/or assist the activities of RAD51, a recombinase which plays a critical role in both DNA repair and meiotic recombination. Yeast two-hybrid screening of a human testis cDNA library revealed a new protein, RAD51AP2 (RAD51 Associated Protein 2), that interacts strongly with RAD51. ...

journal_title：Nucleic acids research

authors： Kovalenko OV,Wiese C,Schild D

更新日期：2006-01-01 00:00:00