Abstract:
:We previously reported that O4-alkyl dTTPs could replace, for short times, dTTP in polymer synthesis [Singer et al., PNAS 83, 26-32, 1986]. The reasons for such early termination of synthesis could be either proofreading or the eventual formation of weakly paired primer termini. Utilizing the known 3'----5' exonucleolytic activity of polymerases, in the absence of dNTPs, enabled us to conclude that, in contrast to the digestibility of poly[d(A-T)] which yielded the expected 3'-mononucleotides, the polymerizing enzymes did not digest O4-methyl dT or its neighbors. The presence of the resistant alpha-phosphorothionate linkage did not prevent measurable digestion of poly[d(A-T)] by the Klenow fragment. This, together with evidence that polymerization of O4-methyl dTTP is favored at low temperatures, supports the model proposed by Ollis et al. [Nature 313, 762-766, 1985] showing independent domains for the two activities in the Klenow fragment.
journal_name
Nucleic Acids Resjournal_title
Nucleic acids researchauthors
Singer Bdoi
10.1093/nar/14.16.6735subject
Has Abstractpub_date
1986-08-26 00:00:00pages
6735-43issue
16eissn
0305-1048issn
1362-4962journal_volume
14pub_type
杂志文章abstract::A terminator of transcription with bidirectional activity has been located between the translation termination codons of the genes tetA and orfL on Tn10. These genes are transcribed towards each other. Each orientation of the intervening sequence is shown to reduce the expression of the lacZ and galK genes when cloned...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/13.12.4227
更新日期:1985-06-25 00:00:00
abstract::OrthoDB is a comprehensive catalog of orthologs, genes inherited by extant species from a single gene in their last common ancestor. In 2016 OrthoDB reached its 9th release, growing to over 22 million genes from over 5000 species, now adding plants, archaea and viruses. In this update we focused on usability of this f...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkw1119
更新日期:2017-01-04 00:00:00
abstract::Methods for increasing the transient level of expression of transfected DNA in cultured Drosophila cells have been examined. Here we show that cells exposed to the steroid hormone 20-hydroxyecdysone for 48h prior to transfection show an 4 to 5 fold increase in the levels of expression of transfected DNA. By analysis, ...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/15.22.9255
更新日期:1987-11-25 00:00:00
abstract::The Dnd (DNA degradation) phenotype, reflecting a novel DNA modification by sulfur in Streptomyces lividans 1326, was strongly aggravated when one (dndB) of the five genes (dndABCDE) controlling it was mutated. Electrophoretic banding patterns of a plasmid (pHZ209), reflecting DNA degradation, displayed a clear change...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkm176
更新日期:2007-01-01 00:00:00
abstract::Cooperative hybridization has been used to establish physical linkage between two loci on a DNA strand. Linkage was detected by hybridization to a new type of high-density oligonucleotide array. Each synthesis location on the array contains a mixture of two different probe sequences. Each of the two probes can hybridi...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/27.6.1485
更新日期:1999-03-15 00:00:00
abstract::Two new crescent-shaped unsymmetrical cyanine dyes have been synthesised and their interactions with DNA have been investigated by different spectroscopic methods. These dyes are analogues to a minor groove binding unsymmetrical cyanine dye, BEBO, recently reported by us. In this dye, the structure of the known interc...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkg821
更新日期:2003-11-01 00:00:00
abstract::Alternative splicing constitutes a major mechanism creating protein diversity in humans. This diversity can result from the alternative skipping of entire exons or by alternative selection of the 5' or 3' splice sites that define the exon boundaries. In this study, we analyze the sequence and evolutionary characterist...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkm603
更新日期:2007-01-01 00:00:00
abstract::The translational regulation of ferritin expression currently represents the only well characterized example for eukaryotic translational control by high affinity interactions between a specific cytoplasmic protein, iron regulatory factor [IRF], and an mRNA-binding site, the iron-responsive element [IRE], located in t...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/20.21.5555
更新日期:1992-11-11 00:00:00
abstract::We describe a rapid fluorescence indicator displacement assay (R-FID) to evaluate the affinity and the selectivity of compounds binding to different DNA structures. We validated the assay using a library of 30 well-known nucleic acid binders containing a variety chemical scaffolds. We used a combination of principal c...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gky019
更新日期:2018-04-20 00:00:00
abstract::The Human Gene and Protein Database (HGPD; http://www.HGPD.jp/) is a unique database that stores information on a set of human Gateway entry clones in addition to protein expression and protein synthesis data. The HGPD was launched in November 2008, and 33,275 human Gateway entry clones have been constructed from the ...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkr1188
更新日期:2012-01-01 00:00:00
abstract::Cultured mammalian cells transduced with the Escherichia coli gene, Ecogpt, synthesize the bacterial enzyme xanthine-guanine phosphoribosyl transferase (XGPT) (1). This paper describes a method for measuring XGPT activity in crude cell extracts by following the conversion of 14C-xanthine (X) to 14C-xanthine monophosph...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/13.8.2921
更新日期:1985-04-25 00:00:00
abstract::We present web servers for analysis of non-coding RNA sequences on the basis of their secondary structures. Software tools for structural multiple sequence alignments, structural pairwise sequence alignments and structural motif findings are available from the integrated web server and the individual stand-alone web s...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkn222
更新日期:2008-07-01 00:00:00
abstract::Non-homologous DNA end joining (NHEJ) is considered the major pathway of double-strand break (DSB) repair in mammalian cells and depends, among other things, on the DNA end-binding Ku70/80 hetero-dimer. To investigate the function of Ku in NHEJ we have compared the ability of cell-free extracts from wild-type CHO-K1 c...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/28.13.2585
更新日期:2000-07-01 00:00:00
abstract::We show that the cAMP receptor protein (Crp) binds to DNA as several different conformers. This situation has precluded discovering a high correlation between any sequence property and binding affinity for proteins that bend DNA. Experimentally quantified affinities of Synechocystis sp. PCC 6803 cAMP receptor protein ...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkr369
更新日期:2011-08-01 00:00:00
abstract::Calf thymus DNA polymerase alpha (pol alpha) and bacteriophage T4 DNA polymerase (pol T4) were exploited as model enzymes to investigate the molecular mechanism of inhibitory action of N2-(p-n-butylphenyl)dGTP (BuPdGTP) and 2-(p-n-butyl-anilino)dATP (BuAdATP) on the BuPdNTP-susceptible alpha polymerase family. Kinetic...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/19.7.1627
更新日期:1991-04-11 00:00:00
abstract::DNA swivel (nicking-rejoining) enzyme activity has been studied in various cell fractions of a human lymphoid cell line. Swivel activity is found only in chromatin and in a nuclear membrane fraction containing DNA and possessing endogenous DNA synthesizing activity. Twenty percent of the total swivel activity and less...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/4.1.223
更新日期:1977-01-01 00:00:00
abstract::I have used a novel single-sided specific polymerase chain reaction (PCR) strategy inspired by ligation-mediated PCR to clone fragments of divergent homeobox genes from a flatworm, the planarian Polycelis nigra. Eight homeobox-containing fragments were amplified, belonging to the Hox, msh, NK-1 and NK-2 classes. Toget...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/24.8.1547
更新日期:1996-04-15 00:00:00
abstract::The European Nucleotide Archive (ENA; http://www.ebi.ac.uk/ena) is Europe's primary nucleotide-sequence repository. The ENA consists of three main databases: the Sequence Read Archive (SRA), the Trace Archive and EMBL-Bank. The objective of ENA is to support and promote the use of nucleotide sequencing as an experimen...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkq967
更新日期:2011-01-01 00:00:00
abstract::It is well established that gene regulation can be achieved through activator and repressor proteins that bind to DNA and switch particular genes on or off, and that complex metabolic networks determine the levels of transcription of a given gene at a given time. Using three complementary computational techniques to s...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkw815
更新日期:2016-11-02 00:00:00
abstract::Fragments of SP6 DNA generated by cleavage with Hpa II or Taq I were cloned into the Cla I site of pBR322 and the recombinant plasmids were screened for the presence of SP6 promoter activity by transcription in vitro with purified SP6 RNA polymerase. Three plasmids having promoter activity and small inserts of SP6 DNA...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/14.8.3521
更新日期:1986-04-25 00:00:00
abstract::siRNAs confer sequence specific and robust silencing of mRNA. By virtue of these properties, siRNAs have become therapeutic candidates for disease intervention. However, their use as therapeutic agents can be hampered by unintended off-target effects by either or both strands of the siRNA duplex. We report here that u...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkq961
更新日期:2011-03-01 00:00:00
abstract::We recently reported that the antitumor triazoloacridone, compound C-1305, is a topoisomerase II poison with unusual properties. In this study we characterize the DNA interactions of C-1305 in vitro, in comparison with other topoisomerase II inhibitors. Our results show that C-1305 binds to DNA by intercalation and po...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gki904
更新日期:2005-10-27 00:00:00
abstract::We examined the influence of the tetratricopeptide repeat factor XAB2 on chromosomal break repair, and found that XAB2 promotes end resection that generates the 3' ssDNA intermediate for homologous recombination (HR). Namely, XAB2 is important for chromosomal double-strand break (DSB) repair via two pathways of HR tha...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkw275
更新日期:2016-07-08 00:00:00
abstract::Eukaryotic cellular mRNAs contain a cap at their 5'-ends, but some viral and cellular mRNAs bypass the cap-dependent mechanism of translation initiation in favor of internal entry of ribosomes at specific RNA sequences. Cap-dependent initiation requires intact initiation factor eIF4G (formerly eIF-4gamma, eIF-4Fgamma ...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/25.2.395
更新日期:1997-01-15 00:00:00
abstract::A soluble cell-free extract containing RNA polymerase III and factors essential for selective transcription of the yeast SUP4-o tRNATyr gene was prepared from Saccharomyces cerevisiae cells. An intragenic promoter for yeast RNA polymerase III was identified within the yeast tRNATyr coding sequence by testing several s...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/10.24.8127
更新日期:1982-12-20 00:00:00
abstract::Gene duplication is an important process in evolution. The availability of genome sequences of a number of organisms has made it possible to conduct comprehensive searches for duplicated genes enabling informative studies of their evolution. We have established the FGF (Fishing Gene Family) program to efficiently sear...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkm426
更新日期:2007-07-01 00:00:00
abstract::The interaction of RNase T1 with calf thymus DNA was studied using uv difference spectroscopy and the effect of the enzyme on DNA melting. There was no indication of RNase T1 binding with native DNA. A prominent difference spectrum for RNase T1 binding with denatured DNA (d-DNA) was observed at pH 5, 25 degrees and lo...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/2.1.11
更新日期:1975-01-01 00:00:00
abstract::The specific function of RNA molecules frequently resides in their seemingly unstructured loop regions. We performed a systematic analysis of RNA loops extracted from experimentally determined three-dimensional structures of RNA molecules. A comprehensive loop-structure data set was created and organized into distinct...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkp1010
更新日期:2010-01-01 00:00:00
abstract::TERC is an RNA component of telomerase. However, TERC is also ubiquitously expressed in most human terminally differentiated cells, which don't have telomerase activity. The function of TERC in these cells is largely unknown. Here, we report that TERC enhances the expression and secretion of inflammatory cytokines by ...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkz584
更新日期:2019-09-05 00:00:00
abstract::Tandem repeats in DNA have been under intensive study for many years, first, as a consequence of their usefulness as genomic markers and DNA fingerprints and more recently as their role in human disease and regulatory processes has become apparent. The Tandem Repeats Database (TRDB) is a public repository of informati...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkl1013
更新日期:2007-01-01 00:00:00