当前位置： SCI文献检索 > NUCLEIC ACIDS RESEARCH期刊下所有文献 > Peptidyl-tRNA hydrolase from Sulfolobus solfataricus.

Peptidyl-tRNA hydrolase from Sulfolobus solfataricus.

Abstract：

:An enzyme capable of liberating functional tRNA(Lys) from Escherichia coli diacetyl-lysyl-tRNA(Lys) was purified from the archae Sulfolobus solfataricus. Contrasting with the specificity of peptidyl- tRNA hydrolase (PTH) from E.coli, the S.solfataricus enzyme readily accepts E.coli formyl-methionyl-tRNA(fMet) as a substrate. N-terminal sequencing of this enzyme identifies a gene that has homologs in the whole archaeal kingdom. Involvement of this gene (SS00175) in the recycling of peptidyl-tRNA is supported by its capacity to complement an E.coli strain lacking PTH activity. The archaeal gene, the product of which appears markedly different from bacterial PTHs, also has homologs in all the available eukaryal genomes. Since most of the eukaryotes already display a bacterial-like PTH gene, this observation suggests the occurrence in many eukaryotes of two distinct PTH activities, either of a bacterial or of an archaeal type. Indeed, the bacterial- and archaeal-like genes encoding the two full-length PTHs of Saccharomyces cerevisiae, YHR189w and YBL057c, respectively, can each rescue the growth of an E.coli strain lacking endogeneous PTH. In vitro assays confirm that the two enzymes ensure the recycling of tRNA(Lys) from diacetyl-lysyl-tRNA(Lys). Finally, the growth of yeast cells in which either YHR189w or YBL057c has been disrupted was compared under various culture conditions. Evidence is presented that YHR189w, the gene encoding a bacterial-like PTH, should be involved in mitochondrial function.

journal_name

Nucleic Acids Res

journal_title

Nucleic acids research

authors

Fromant M,Ferri-Fioni ML,Plateau P,Blanquet S

doi

10.1093/nar/gkg428

keywords:

subject

Has Abstract

pub_date

2003-06-15 00:00:00

pages

3227-35

issue

12

eissn

0305-1048

issn

1362-4962

journal_volume

31

pub_type

杂志文章

相关文献

NUCLEIC ACIDS RESEARCH文献大全
  • PHD finger protein 1 (PHF1) is a novel reader for histone H4R3 symmetric dimethylation and coordinates with PRMT5-WDR77/CRL4B complex to promote tumorigenesis.

    abstract：:Histone post-translational modifications regulate chromatin structure and function largely through interactions with effector proteins that often contain multiple histone-binding domains. PHF1 [plant homeodomain (PHD) finger protein 1], which contains two kinds of histone reader modules, a Tudor domain and two PHD fin...

    journal_title：Nucleic acids research

    pub_type： 杂志文章

    doi：10.1093/nar/gky461

    authors： Liu R,Gao J,Yang Y,Qiu R,Zheng Y,Huang W,Zeng Y,Hou Y,Wang S,Leng S,Feng D,Yu W,Sun G,Shi H,Teng X,Wang Y

    更新日期：2018-07-27 00:00:00

  • Structural organization and differential expression of rice alpha-amylase genes.

    abstract：:Rice alpha-amylases are encoded by a multigene family that has previously been classified into 5 hybridization groups. DNA sequence and Southern blot analysis identified three genes (RAmy1A, RAmy1B and RAmy1C) in Group 1 with DNA sequence identity of at least 90%. Hybridization Group 2 is represented by only one gene,...

    journal_title：Nucleic acids research

    pub_type： 杂志文章

    doi：10.1093/nar/18.23.7007

    authors： Huang N,Koizumi N,Reinl S,Rodriguez RL

    更新日期：1990-12-11 00:00:00

  • Impairing the production of ribosomal RNA activates mammalian target of rapamycin complex 1 signalling and downstream translation factors.

    abstract：:Ribosome biogenesis is a key process for maintaining protein synthetic capacity in dividing or growing cells, and requires coordinated production of ribosomal proteins and ribosomal RNA (rRNA), including the processing of the latter. Signalling through mammalian target of rapamycin complex 1 (mTORC1) activates all the...

    journal_title：Nucleic acids research

    pub_type： 杂志文章

    doi：10.1093/nar/gku130

    authors： Liu R,Iadevaia V,Averous J,Taylor PM,Zhang Z,Proud CG

    更新日期：2014-04-01 00:00:00

  • Genome-wide identification of genes directly regulated by the pleiotropic transcription factor Spx in Bacillus subtilis.

    abstract：:The transcriptional regulator Spx plays a key role in maintaining the redox homeostasis of Bacillus subtilis cells exposed to disulfide stress. Defects in Spx were previously shown to lead to differential expression of numerous genes but direct and indirect regulatory effects could not be distinguished. Here we identi...

    journal_title：Nucleic acids research

    pub_type： 杂志文章

    doi：10.1093/nar/gks755

    authors： Rochat T,Nicolas P,Delumeau O,Rabatinová A,Korelusová J,Leduc A,Bessières P,Dervyn E,Krásny L,Noirot P

    更新日期：2012-10-01 00:00:00

  • TEG-1 CD2BP2 controls miRNA levels by regulating miRISC stability in C. elegans and human cells.

    abstract：:MiRNAs post-transcriptionally regulate gene expression by recruiting the miRNA-induced silencing complex (miRISC) to target mRNAs. However, the mechanisms by which miRISC components are maintained at appropriate levels for proper function are largely unknown. Here, we demonstrate that Caenorhabditis elegans TEG-1 regu...

    journal_title：Nucleic acids research

    pub_type： 杂志文章

    doi：10.1093/nar/gkw836

    authors： Wang C,Gupta P,Fressigne L,Bossé GD,Wang X,Simard MJ,Hansen D

    更新日期：2017-02-17 00:00:00

  • Functional analysis of the tobacco mosaic virus tRNA-like structure in cytoplasmic gene regulation.

    abstract：:The 3'-untranslated region (UTR) of tobacco mosaic virus (TMV), which terminates in a tRNA-like structure, functionally substitutes for a poly(A) tail in both plant and animal cells. The addition of the TMV 3'-UTR to chimeric mRNA constructs increases their expression up to 100-fold, increasing both translational effi...

    journal_title：Nucleic acids research

    pub_type： 杂志文章

    doi：10.1093/nar/19.18.5031

    authors： Gallie DR,Feder JN,Schimke RT,Walbot V

    更新日期：1991-09-25 00:00:00

  • RNA editing in the acceptor stem of squid mitochondrial tRNA(Tyr).

    abstract：:In squid (Loligo bleekeri) mitochondria, the two 3'-terminal nucleotides (G72-G73) of the tRNA(Tyr) gene overlap with the two 5'-terminal nucleotides (G1-G2) of the downstream tRNA(Cys) gene. To elucidate the processing mechanism(s) of the tRNA molecules derived from this region, tRNAs were analyzed by sequencing cDNA...

    journal_title：Nucleic acids research

    pub_type： 杂志文章

    doi：10.1093/nar/24.24.4987

    authors： Tomita K,Ueda T,Watanabe K

    更新日期：1996-12-15 00:00:00

  • Quantitation of supercoiled circular content in plasmid DNA solutions using a fluorescence-based method.

    abstract：:A method for quantifying the proportion of supercoiled circular (SC) forms in DNA solutions is described. The method (SCFluo) takes advantage of the reversible denaturation property of SC forms and the high specificity of the PicoGreen fluorochrome for double-stranded (ds)DNA. Fluorescence values of forms capable of r...

    journal_title：Nucleic acids research

    pub_type： 杂志文章

    doi：10.1093/nar/28.12.e57

    authors： Levy MS,Lotfian P,O'Kennedy R,Lo-Yim MY,Shamlou PA

    更新日期：2000-06-15 00:00:00

  • A periodic pattern of mRNA secondary structure created by the genetic code.

    abstract：:Single-stranded mRNA molecules form secondary structures through complementary self-interactions. Several hypotheses have been proposed on the relationship between the nucleotide sequence, encoded amino acid sequence and mRNA secondary structure. We performed the first transcriptome-wide in silico analysis of the huma...

    journal_title：Nucleic acids research

    pub_type： 杂志文章

    doi：10.1093/nar/gkl287

    authors： Shabalina SA,Ogurtsov AY,Spiridonov NA

    更新日期：2006-05-08 00:00:00

  • Role of interaction energy in the specificity of transcription. II-The Watson Crick A-U base pair template.

    abstract：:In continuation with work regarding the evaluation of the energy of association of various RNA bases with various base pairs, the results of the computations of the electrostatic interaction energy of A-U base pair with four RNA bases viz. Adenine, Uracil, Guanine and Cytosine have been reported. Non bonded induced po...

    journal_title：Nucleic acids research

    pub_type： 杂志文章

    doi：10.1093/nar/8.17.3983

    authors： Sanyal NK,Kumar U,Roychoudhury M

    更新日期：1980-09-11 00:00:00

  • Investigating the sequence-dependent mechanical properties of DNA nicks for applications in twisted DNA nanostructure design.

    abstract：:DNA nick can be used as a design motif in programming the shape and reconfigurable deformation of synthetic DNA nanostructures, but its mechanical properties have rarely been systematically characterized at the level of base sequences. Here, we investigated sequence-dependent mechanical properties of DNA nicks through...

    journal_title：Nucleic acids research

    pub_type： 杂志文章

    doi：10.1093/nar/gky1189

    authors： Lee JY,Kim YJ,Lee C,Lee JG,Yagyu H,Tabata O,Kim DN

    更新日期：2019-01-10 00:00:00

  • Overexpression of the base excision repair NTHL1 glycosylase causes genomic instability and early cellular hallmarks of cancer.

    abstract：:Base excision repair (BER), which is initiated by DNA N-glycosylase proteins, is the frontline for repairing potentially mutagenic DNA base damage. The NTHL1 glycosylase, which excises DNA base damage caused by reactive oxygen species, is thought to be a tumor suppressor. However, in addition to NTHL1 loss-of-function...

    journal_title：Nucleic acids research

    pub_type： 杂志文章

    doi：10.1093/nar/gky162

    authors： Limpose KL,Trego KS,Li Z,Leung SW,Sarker AH,Shah JA,Ramalingam SS,Werner EM,Dynan WS,Cooper PK,Corbett AH,Doetsch PW

    更新日期：2018-05-18 00:00:00

  • Nucleotide sequence of cucumber pale fruit viroid: homology to hop stunt viroid.

    abstract：:Double stranded cDNA of cucumber pale fruit viroid ( CPFV ) has been cloned by the method of Okayama and Berg (Mol.Cell.Biol.2,161-170 (1982] and the complete nucleotide sequence was established. The covalently closed circular molecules of single-stranded CPFV RNA consists of 303 nucleotides. The nucleotide sequence o...

    journal_title：Nucleic acids research

    pub_type： 杂志文章

    doi：10.1093/nar/12.8.3427

    authors： Sano T,Uyeda I,Shikata E,Ohno T,Okada Y

    更新日期：1984-04-25 00:00:00

  • Human Rad51 protein displays enhanced homologous pairing of DNA sequences resembling those at genetically unstable loci.

    abstract：:DNA strand exchange, the central step of homologous recombination, is considered to occur approximately independently of DNA sequence content. However, certain prokaryotic and eukaryotic genomic loci display either an enhanced or reduced frequency of genetic exchange. Here we show that the Homo sapiens DNA strand exch...

    journal_title：Nucleic acids research

    pub_type： 杂志文章

    doi：10.1093/nar/gkl355

    authors： Seitz EM,Kowalczykowski SC

    更新日期：2006-05-24 00:00:00

  • Mechanistic studies on the DNA linking activity of Epstein-Barr nuclear antigen 1.

    abstract：:The DNA replication, plasmid segregation and transactivation functions of Epstein-Barr nuclear antigen 1 (EBNA1) require the binding of EBNA1 to specific DNA recognition sites in the two non-contiguous functional elements of the Epstein-Barr virus latent origin of replication, oriP . EBNA1 molecules bound to these ele...

    journal_title：Nucleic acids research

    pub_type： 杂志文章

    doi：10.1093/nar/26.19.4462

    authors： Avolio-Hunter TM,Frappier L

    更新日期：1998-10-01 00:00:00

  • Structural basis of sequestration of the anti-Shine-Dalgarno sequence in the Bacteroidetes ribosome.

    abstract：:Genomic studies have indicated that certain bacterial lineages such as the Bacteroidetes lack Shine-Dalgarno (SD) sequences, and yet with few exceptions ribosomes of these organisms carry the canonical anti-SD (ASD) sequence. Here, we show that ribosomes purified from Flavobacterium johnsoniae, a representative of the...

    journal_title：Nucleic acids research

    pub_type： 杂志文章

    doi：10.1093/nar/gkaa1195

    authors： Jha V,Roy B,Jahagirdar D,McNutt ZA,Shatoff EA,Boleratz BL,Watkins DE,Bundschuh R,Basu K,Ortega J,Fredrick K

    更新日期：2021-01-11 00:00:00

  • A recombination based method to rapidly assess specificity of two-hybrid clones in yeast.

    abstract：:The yeast two-hybrid system is frequently used to identify protein-protein interactions. Confirming the specificity of candidate clones requires separation and isolation of yeast plasmids, propagation in bacteria and testing combinations of DNA-binding and activation domain hybrids in yeast. In order to simplify this ...

    journal_title：Nucleic acids research

    pub_type： 杂志文章

    doi：10.1093/nar/26.9.2252

    authors： Petermann R,Mossier BM,Aryee DN,Kovar H

    更新日期：1998-05-01 00:00:00

  • Génolevures: protein families and synteny among complete hemiascomycetous yeast proteomes and genomes.

    abstract：:The Génolevures online database (http://cbi.labri.fr/Genolevures/ and http://genolevures.org/) provides exploratory tools and curated data sets relative to nine complete and seven partial genome sequences determined and manually annotated by the Génolevures Consortium, to facilitate comparative genomic studies of Hemi...

    journal_title：Nucleic acids research

    pub_type： 杂志文章

    doi：10.1093/nar/gkn859

    authors： Sherman DJ,Martin T,Nikolski M,Cayla C,Souciet JL,Durrens P,Génolevures Consortium.

    更新日期：2009-01-01 00:00:00

  • Methylation and restriction endonuclease cleavage of linear Z-DNA in the presence of hexamminecobalt (III) ions.

    abstract：:These studies employed the synthetic linear DNA, poly dGdC, in the B and cobalt hexammine chloride (Co)-induced Z form to determine the effect of conformation on protein-DNA interactions. The rate of the reaction of the restriction endonucleases, Hha I and Cfo I, are reduced with Z DNA as compared to B DNA. The abilit...

    journal_title：Nucleic acids research

    pub_type： 杂志文章

    doi：10.1093/nar/14.18.7237

    authors： Soslau G,Parker J,Nelson JW

    更新日期：1986-09-25 00:00:00

  • In vitro site-directed mutagenesis with synthetic DNA oligonucleotides yields unexpected deletions and insertions at high frequency.

    abstract：:We have used in vitro site-directed mutagenesis with synthetic DNA oligonucleotides to introduce single nucleotide mutations in yeast mtDNA. In addition to the expected DNA alterations we also recovered with high frequency mutants with large deletions and insertions which arose through interaction with the synthetic D...

    journal_title：Nucleic acids research

    pub_type： 杂志文章

    doi：10.1093/nar/11.24.8595

    authors： Osinga KA,Van der Bliek AM,Van der Horst G,Groot Koerkamp MJ,Tabak HF,Veeneman GH,Van Boom JH

    更新日期：1983-12-20 00:00:00

  • Mutational analysis of an essential binding site for the U3 snoRNA in the 5' external transcribed spacer of yeast pre-rRNA.

    abstract：:The small nucleolar RNA U3 is essential for viability in yeast. We have previously shown that U3 can be cross-linked in vivo to the pre-rRNA in the 5' external transcribed spacer (ETS), at +470. This ETS region contains 10 nucleotides of perfect complementarity to U3. In a genetic background where the mutated rDNA is ...

    journal_title：Nucleic acids research

    pub_type： 杂志文章

    doi：10.1093/nar/22.20.4057

    authors： Beltrame M,Henry Y,Tollervey D

    更新日期：1994-10-11 00:00:00

  • Inter-individual variation of DNA methylation and its implications for large-scale epigenome mapping.

    abstract：:Genomic DNA methylation profiles exhibit substantial variation within the human population, with important functional implications for gene regulation. So far little is known about the characteristics and determinants of DNA methylation variation among healthy individuals. We performed bioinformatic analysis of high-r...

    journal_title：Nucleic acids research

    pub_type： 杂志文章

    doi：10.1093/nar/gkn122

    authors： Bock C,Walter J,Paulsen M,Lengauer T

    更新日期：2008-06-01 00:00:00

  • Pharos: Collating protein information to shed light on the druggable genome.

    abstract：:The 'druggable genome' encompasses several protein families, but only a subset of targets within them have attracted significant research attention and thus have information about them publicly available. The Illuminating the Druggable Genome (IDG) program was initiated in 2014, has the goal of developing experimental...

    journal_title：Nucleic acids research

    pub_type： 杂志文章

    doi：10.1093/nar/gkw1072

    authors： Nguyen DT,Mathias S,Bologa C,Brunak S,Fernandez N,Gaulton A,Hersey A,Holmes J,Jensen LJ,Karlsson A,Liu G,Ma'ayan A,Mandava G,Mani S,Mehta S,Overington J,Patel J,Rouillard AD,Schürer S,Sheils T,Simeonov A,Sklar L

    更新日期：2017-01-04 00:00:00

  • Quantitation of DNA double-strand break resection intermediates in human cells.

    abstract：:5' strand resection at DNA double strand breaks (DSBs) is critical for homologous recombination (HR) and genomic stability. Here we develop a novel method to quantitatively measure single-stranded DNA intermediates in human cells and find that the 5' strand at endonuclease-generated break sites is resected up to 3.5 k...

    journal_title：Nucleic acids research

    pub_type： 杂志文章

    doi：10.1093/nar/gkt1309

    authors： Zhou Y,Caron P,Legube G,Paull TT

    更新日期：2014-02-01 00:00:00

  • Promoter-enhancer looping at the PPARγ2 locus during adipogenic differentiation requires the Prmt5 methyltransferase.

    abstract：:PPARγ2 is a critical lineage-determining transcription factor that is essential for adipogenic differentiation. Here we report characterization of the three-dimensional structure of the PPARγ2 locus after the onset of adipogenic differentiation and the mechanisms by which it forms. We identified a differentiation-depe...

    journal_title：Nucleic acids research

    pub_type： 杂志文章

    doi：10.1093/nar/gkw129

    authors： LeBlanc SE,Wu Q,Lamba P,Sif S,Imbalzano AN

    更新日期：2016-06-20 00:00:00

  • Quantitative evaluation of Escherichia coli host strains for tolerance to cytosine methylation in plasmid and phage recombinants.

    abstract：:Many strains of E. coli K12 restrict DNA containing cytosine methylation such as that present in plant and animal genomes. Such restriction can severely inhibit the efficiency of cloning genomic DNAs. We have quantitatively evaluated a total of 39 E. coli strains for their tolerance to cytosine methylation in phage an...

    journal_title：Nucleic acids research

    pub_type： 杂志文章

    doi：10.1093/nar/17.9.3469

    authors： Woodcock DM,Crowther PJ,Doherty J,Jefferson S,DeCruz E,Noyer-Weidner M,Smith SS,Michael MZ,Graham MW

    更新日期：1989-05-11 00:00:00

  • Control of cell division in Escherichia coli. DNA sequence of dicA and of a second gene complementing mutation dicA1, dicC.

    abstract：:A mutation in a gene dicA of Escherichia coli leads to temperature-sensitive cell division, by allowing expression of a nearby division inhibition gene dicB (1). We have now established the sequence of the DicA region and identified DicA as a 15.5 KD protein. A second gene dicC transcribed divergently from dicA and co...

    journal_title：Nucleic acids research

    pub_type： 杂志文章

    doi：10.1093/nar/14.17.6821

    authors： Béjar S,Cam K,Bouché JP

    更新日期：1986-09-11 00:00:00

  • Partial inhibition of histone deacetylase in active chromatin by HMG 14 and HMG 17.

    abstract：:Digestion of isolated Friend erythroleukemic cell nuclei with DNase I under conditions which selectively destroy the DNA of transcriptionally "active" genes releases into the supernatant fraction proteins of the non-histone "High Mobility Group" (HMGs). Two of these, HMG-14 and HMG-17(identified by solubility in trich...

    journal_title：Nucleic acids research

    pub_type： 杂志文章

    doi：10.1093/nar/8.9.1947

    authors： Reeves R,Candido EP

    更新日期：1980-05-10 00:00:00

  • MEIOK21: a new component of meiotic recombination bridges required for spermatogenesis.

    abstract：:Repair of DNA double-strand breaks (DSBs) with homologous chromosomes is a hallmark of meiosis that is mediated by recombination 'bridges' between homolog axes. This process requires cooperation of DMC1 and RAD51 to promote homology search and strand exchange. The mechanism(s) regulating DMC1/RAD51-ssDNA nucleoprotein...

    journal_title：Nucleic acids research

    pub_type： 杂志文章

    doi：10.1093/nar/gkaa406

    authors： Shang Y,Huang T,Liu H,Liu Y,Liang H,Yu X,Li M,Zhai B,Yang X,Wei Y,Wang G,Chen Z,Wang S,Zhang L

    更新日期：2020-07-09 00:00:00

  • The PathoYeastract database: an information system for the analysis of gene and genomic transcription regulation in pathogenic yeasts.

    abstract：:We present the PATHOgenic YEAst Search for Transcriptional Regulators And Consensus Tracking (PathoYeastract - http://pathoyeastract.org) database, a tool for the analysis and prediction of transcription regulatory associations at the gene and genomic levels in the pathogenic yeasts Candida albicans and C. glabrata Up...

    journal_title：Nucleic acids research

    pub_type： 杂志文章

    doi：10.1093/nar/gkw817

    authors： Monteiro PT,Pais P,Costa C,Manna S,Sá-Correia I,Teixeira MC

    更新日期：2017-01-04 00:00:00