A novel family of retrotransposon-like elements in Xenopus laevis with a transcript inducible by two growth factors.

abstract：:The synthesis of 1-(beta-D-2'-deoxyribosyl)-2-pyrimidinone (dK) and its 5-methyl derivative (d5) from 2'-deoxycytidine or 2'-deoxythymidine, respectively, via silver oxide oxidation of 4-hydrazinopyrimidines is described. The necessary hydrazine substituted pyrimidine nucleosides have been prepared by transamination o...

journal_title：Nucleic acids research

authors： Gildea B,McLaughlin LW

更新日期：1989-03-25 00:00:00

abstract：:No analogous nucleoside triphosphate was found which acts as well as ATP in binding to and supporting catalysis of leucyl-tRNA synthetase from Escherichia coli MRE 600. However, there are numerous nucleotides which are able to replace ATP, but with lower efficiency. The 6-amino group of the adenine ring and the 2'-hyd...

journal_title：Nucleic acids research

authors： Marutzky R,Flossdorf J,Kula MR

更新日期：1976-08-01 00:00:00

abstract：:IntAct is an open-source, open data molecular interaction database populated by data either curated from the literature or from direct data depositions. Two levels of curation are now available within the database, with both IMEx-level annotation and less detailed MIMIx-compatible entries currently supported. As from ...

journal_title：Nucleic acids research

authors： Kerrien S,Aranda B,Breuza L,Bridge A,Broackes-Carter F,Chen C,Duesbury M,Dumousseau M,Feuermann M,Hinz U,Jandrasits C,Jimenez RC,Khadake J,Mahadevan U,Masson P,Pedruzzi I,Pfeiffenberger E,Porras P,Raghunath A,Roeche

更新日期：2012-01-01 00:00:00

abstract：:Molecules of single-stranded ribosomal RNA and double-stranded replicative form of phage f2 RNA (dsRNA) adopt a compact form in solutions, containing sufficiently high concentrations of salt (NaCl) and polymer (PEG). However, only in the cases of native dsRNA molecules the compact particles are characterized by a regu...

journal_title：Nucleic acids research

authors： Evdokimov YM,Pyatigorskaya TL,Kadikov VA,Polyvtsev OF,Doskocil J,Koudelka J,Varshavsky YM

更新日期：1976-06-01 00:00:00

abstract：:Synthetic deoxyoligonucleotides have been 5'-aminoalkylated at the end of step-wise synthesis on the polymer support. This was achieved through the activation of the 5'-hydroxyl group as its 5'-imidazolyl derivative using carbonyldiimidazole, which was subsequently displaced with hexamethylene diamine to yield the tit...

journal_title：Nucleic acids research

authors： Wachter L,Jablonski JA,Ramachandran KL

更新日期：1986-10-24 00:00:00

abstract：:Lysinomicin, a naturally-occurring pseudodisaccharide, inhibits translation in prokaryotes. We report that lysinomicin (and three related compounds) are able to inhibit the self-splicing of group I introns, thus identifying pseudodisaccharides as a novel class of group I intron splicing inhibitors. Lysinomicin inhibit...

journal_title：Nucleic acids research

authors： Rogers J,Davies J

更新日期：1994-11-25 00:00:00

abstract：:H3K4me3 is a histone modification that accumulates at the transcription-start site (TSS) of active genes and is known to be important for transcription activation. The way in which H3K4me3 is regulated at TSS and the actual molecular basis of its contribution to transcription remain largely unanswered. To address thes...

journal_title：Nucleic acids research

authors： Lloret-Llinares M,Pérez-Lluch S,Rossell D,Morán T,Ponsa-Cobas J,Auer H,Corominas M,Azorín F

更新日期：2012-10-01 00:00:00

abstract：:HeLa cell OTF-1 has been purified on the basis of its DNA binding activity and used to raise a polyclonal rabbit antiserum. This antiserum is shown to recognize both native and denatured OTF-1 from both human and a similar protein from Xenopus culture cells, but to react either more weakly or not at all with the lymph...

journal_title：Nucleic acids research

authors： Pruijn JM,van der Vliet PC,Dathan NA,Mattaj IW

更新日期：1989-03-11 00:00:00

abstract：:A new procedure has been developed for the efficient cloning of complex PCR mixtures, resulting in libraries exclusively consisting of recombinant clones. Recombinants are generated between PCR products and a PCR-amplified plasmid vector. The procedure does not require the use of restriction enzymes, T4 DNA ligase or ...

journal_title：Nucleic acids research

authors： Aslanidis C,de Jong PJ

更新日期：1990-10-25 00:00:00

abstract：:A synthetic gene encoding the Schizophyllum commune xylanase XynA was constructed by a novel PCR-based procedure. Three long oligonucleotides were synthesized and used in combination with flanking PCR primers to generate a 607 base pair gene which contained 31 unique locations for restriction enzyme cleavage. The amin...

journal_title：Nucleic acids research

authors： Graham RW,Atkinson T,Kilburn DG,Miller RC Jr,Warren RA

更新日期：1993-10-25 00:00:00

abstract：:Uracil-Specific Exision Reagent (USER) fusion is a recently developed technique that allows for assembly of multiple DNA fragments in a few simple steps. However, designing primers for USER fusion is both tedious and time consuming. Here, we present the Primer Help for USER (PHUSER) software, a novel tool for designin...

journal_title：Nucleic acids research

authors： Olsen LR,Hansen NB,Bonde MT,Genee HJ,Holm DK,Carlsen S,Hansen BG,Patil KR,Mortensen UH,Wernersson R

更新日期：2011-07-01 00:00:00

abstract：:Multifunctional proteins challenge the conventional 'one protein-one function' paradigm. Here we note apparent multifunctional proteins with nucleic acid partners, tabulating eight examples. We then focus on eight additional cases of transcription factors that bind double-stranded DNA with sequence specificity, but th...

journal_title：Nucleic acids research

authors： Cassiday LA,Maher LJ 3rd

更新日期：2002-10-01 00:00:00

abstract：:In this paper we focus on the detection of differentially expressed genes according to changes in hybridization signals using statistical tests. These tests were applied to 14 208 zebrafish cDNA clones that were immobilized on a nylon support and hybridized with radioactively labeled target mRNA from wild-type and lit...

journal_title：Nucleic acids research

authors： Herwig R,Aanstad P,Clark M,Lehrach H

更新日期：2001-12-01 00:00:00

abstract：:Alignment-based programs are valuable tools for finding potential homologs in genome sequences. Previously, it has been shown that partition function posterior probabilities attuned to local alignment achieve a high accuracy in identifying distantly similar non-coding RNA sequences that are hidden in a large genome. H...

journal_title：Nucleic acids research

authors： Chikkagoudar S,Livesay DR,Roshan U

更新日期：2010-07-01 00:00:00

abstract：:The Transcription Regulatory Regions Database (TRRD) is a curated database designed for accumulation of experimental data on extended regulatory regions of eukaryotic genes, the regulatory elements they contain, i.e., transcription factor binding sites, promoters, enhancers, silencers, etc., and expression patterns of...

journal_title：Nucleic acids research

authors： Kolchanov NA,Ananko EA,Podkolodnaya OA,Ignatieva EV,Stepanenko IL,Kel-Margoulis OV,Kel AE,Merkulova TI,Goryachkovskaya TN,Busygina TV,Kolpakov FA,Podkolodny NL,Naumochkin AN,Romashchenko AG

更新日期：1999-01-01 00:00:00

abstract：:Three clones of non-repetitive sequences and six clones containing repetitive sequences were obtained from micronuclear DNA of Tetrahymena thermophila. All the non-repetitive and three repetitive sequences had the same organization in micro- and macronuclear DNAs as revealed by blot hybridization. On the other hand, t...

journal_title：Nucleic acids research

authors： Iwamura Y,Sakai M,Muramatsu M

更新日期：1982-07-24 00:00:00

abstract：:Interleukin 2 (IL2) mRNA has a short half-life in the cytoplasm of T lymphocytes, relative to most mRNA. We have discovered a candidate ribonuclease to account for the rapid turnover of IL2 mRNA in the cytosol of the human T lymphocyte cell line Jurkat. In partially purified form, this RNase is about 7 times as active...

journal_title：Nucleic acids research

authors： Hua J,Garner R,Paetkau V

更新日期：1993-01-11 00:00:00

abstract：:From the general views of the eukaryotic transcription systems, L1 (or L1-like) retrotransposons that encode some proteins are unusual. L1, unlike other protein-coding elements, is transcribed through an internal promoter. And the L1 internal promoter, unlike other internal promoters, is thought to be RNA polymerase I...

journal_title：Nucleic acids research

authors： Kurose K,Hata K,Hattori M,Sakaki Y

更新日期：1995-09-25 00:00:00

abstract：:RNA thermometers control the translation of several heat shock and virulence genes by their temperature-sensitive structural transitions. Changes in the structure and dynamics of MiniROSE RNA, which regulates translation in the temperature range of 20-45°C, were studied by site specifically replacing seven adenine res...

journal_title：Nucleic acids research

authors： Narayan S,Kombrabail MH,Das S,Singh H,Chary KV,Rao BJ,Krishnamoorthy G

更新日期：2015-01-01 00:00:00

abstract：:Synthetic RNA formulations and viral vectors are the two main approaches for delivering small therapeutic RNA to human cells. Here we report findings supporting an alternative strategy in which an endogenous human RNA polymerase (RNAP) is harnessed to make RNA hairpin-containing small RNA from synthetic single-strande...

journal_title：Nucleic acids research

authors： Seidl CI,Lama L,Ryan K

更新日期：2013-02-01 00:00:00

abstract：:An important problem in genomics is automatically clustering homologous proteins when only sequence information is available. Most methods for clustering proteins are local, and are based on simply thresholding a measure related to sequence distance. We first show how locality limits the performance of such methods by...

journal_title：Nucleic acids research

authors： Paccanaro A,Casbon JA,Saqi MA

更新日期：2006-03-17 00:00:00

abstract：:An updated list of DNA sequences cloned from the human genome over the past year (1991) is presented. Intended as a guide to clone availability, this list includes published reports of cDNA, genomic and synthetic probes comprising both gene and pseudogene sequences. ...

journal_title：Nucleic acids research

authors： Schmidtke J,Cooper DN

更新日期：1992-05-11 00:00:00

abstract：:We have isolated a second gene (MLS1), which in addition to DAL7, encodes malate synthase from S. cerevisiae. Expression of the two genes is specific for their physiological roles in carbon and nitrogen metabolism. Expression of MLS1, which participates in the utilization of non-fermentable carbon sources, is sensitiv...

journal_title：Nucleic acids research

authors： Hartig A,Simon MM,Schuster T,Daugherty JR,Yoo HS,Cooper TG

更新日期：1992-11-11 00:00:00

abstract：:Oriented DNA films prepared by the wet-spinning technique have been complexed with several ligands: the anthracycline antibiotic violamycin BI, the dipeptide L-carnosine, and the oligopeptide antibiotic netropsin. The formation of the DNA-ligand complexes is accompanied by dramatic changes of the conformational flexib...

journal_title：Nucleic acids research

authors： Fritzsche H,Rupprecht A,Richter M

更新日期：1984-12-11 00:00:00

abstract：:Despite systematic approaches to mapping networks of genetic interactions in Saccharomyces cerevisiae, exploration of genetic interactions on a genome-wide scale has been limited. The S. cerevisiae haploid genome has 110 regions that are longer than 10 kb but harbor only non-essential genes. Here, we attempted to dele...

journal_title：Nucleic acids research

authors： Kaboli S,Yamakawa T,Sunada K,Takagaki T,Sasano Y,Sugiyama M,Kaneko Y,Harashima S

更新日期：2014-09-01 00:00:00

abstract：:Translational repression (TR) plays an important role in post-transcriptional regulation of gene expression and embryonic development in metazoans. TR also regulates the expression of a subset of the cytoplasmic mRNA population during development of fertilized female gametes of the unicellular malaria parasite, Plasmo...

journal_title：Nucleic acids research

authors： Braks JA,Mair GR,Franke-Fayard B,Janse CJ,Waters AP

更新日期：2008-03-01 00:00:00

abstract：:Many infectious diseases are caused by viral infections, and in particular by RNA viruses such as MERS, Ebola and Zika. To understand viral disease, detection and identification of these viruses are essential. Although PCR is widely used for rapid virus identification due to its low cost and high sensitivity and speci...

journal_title：Nucleic acids research

authors： Kim H,Kang N,An K,Kim D,Koo J,Kim MS

更新日期：2017-01-04 00:00:00

abstract：:The replication control circuit of the FII plasmids R1, R100 and R6-5 involves a small untranslated RNA molecule of about 93 nucleotides. This RNA, designated CopA RNA in plasmid R1, acts in vivo as a post-transcriptional inhibitor of the expression of the RepA protein whose synthesis is required for each round of pla...

journal_title：Nucleic acids research

authors： Gerhart E,Wagner H,Nordström K

更新日期：1986-03-25 00:00:00

abstract：:Small nucleolar RNAs (snoRNAs) are among the first discovered and most extensively studied group of small non-coding RNA. However, most studies focused on a small subset of snoRNAs that guide the modification of ribosomal RNA. In this study, we annotated the expression pattern of all box C/D snoRNAs in normal and canc...

journal_title：Nucleic acids research

authors： Deschamps-Francoeur G,Garneau D,Dupuis-Sandoval F,Roy A,Frappier M,Catala M,Couture S,Barbe-Marcoux M,Abou-Elela S,Scott MS

更新日期：2014-09-01 00:00:00

abstract：:Nucleotide excision DNA repair is mechanistically conserved across all kingdoms of life. In prokaryotes, this multi-enzyme process requires six proteins: UvrA-D, DNA polymerase I and DNA ligase. To examine how UvrC locates the UvrB-DNA pre-incision complex at a site of damage, we have labeled UvrB and UvrC with differ...

journal_title：Nucleic acids research

authors： Hughes CD,Wang H,Ghodke H,Simons M,Towheed A,Peng Y,Van Houten B,Kad NM

更新日期：2013-05-01 00:00:00