Abstract:
:The mitochondrial DNA of Neurospora crassa has a heterogeneous intramolecular base distribution. A contiguous piece, representing at least 30% of the total genome, has a G+C content that is 6% lower than the overall G+C content of the DNA. The genes for both ribosomal RNAs are contained in the remaining, relatively G+C rich, part of the genome.
journal_name
Nucleic Acids Resjournal_title
Nucleic acids researchauthors
Terpstra P,Holtrop M,Kroon Adoi
10.1093/nar/4.1.129keywords:
subject
Has Abstractpub_date
1977-01-01 00:00:00pages
129-39issue
1eissn
0305-1048issn
1362-4962journal_volume
4pub_type
杂志文章abstract::For the analysis of protein-DNA interactions by coupled gel-shift/footprinting, DNA fragments need to be extracted from polyacrylamide gels and subsequently separated on high resolution gels. Due to impurities in the extracted DNA, single nucleotide resolution is frequently not achieved. We now describe an improved ex...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/25.2.453
更新日期:1997-01-15 00:00:00
abstract::Decay of (125)I produces a shower of low energy electrons (Auger electrons) that cause strand breaks in DNA in a distance-dependent manner with 90% of the breaks located within 10 bp from the decay site. We studied strand breaks in RNA molecules produced by decay of (125)I incorporated into complementary DNA oligonucl...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkf622
更新日期:2002-11-15 00:00:00
abstract::Wild type p53 assembles into a latent multiprotein complex which can be activated for sequence-specific DNA binding in vitro by proteins targeting the carboxy-terminal domain. Using an optimized system coupling the post-translational modification of wild type p53 to activation of sequence specific DNA binding, we exam...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/21.14.3167
更新日期:1993-07-11 00:00:00
abstract::The human thrombopoietin (TPO) gene, which codes for the principal cytokine involved in platelet maturation, shows a peculiar alternative splicing of its last exon, where an intra-exonic 116 nt alternative intron is spliced out in a fraction of its mRNA. To characterize the molecular mechanism underlying this alternat...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/29.4.886
更新日期:2001-02-15 00:00:00
abstract::In this paper, we report the cloning and sequencing of the C. elegans histidyl-tRNA synthetase gene. The complete genomic sequence, and most of the cDNA sequence, of this gene is now determined. The gene size including flanking and coding regions is 2230 nucleotides long. Three small introns (45-50 bp long) are found ...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/21.18.4344
更新日期:1993-09-11 00:00:00
abstract::Recently, we described a method for multiplex genome editing by natural transformation (MuGENT). Mutant constructs for MuGENT require large arms of homology (>2000 bp) surrounding each genome edit, which necessitates laborious in vitro DNA splicing. In Vibrio cholerae, we uncover that this requirement is due to cytopl...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkx496
更新日期:2017-07-07 00:00:00
abstract::Site-specific DNA double-strand breaks have been used to generate knock-in through the homology-dependent or -independent pathway. However, low efficiency and accompanying negative impacts such as undesirable indels or tumorigenic potential remain problematic. In this study, we present an enhanced reduced-risk genome ...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkaa195
更新日期:2020-06-04 00:00:00
abstract::Increased cytochrome P450 2E1 (CYP2E1) expression is the main cause of oxidative stress, which exacerbates alcoholic liver diseases (ALDs). Estrogen-related receptor gamma (ERRγ) induces CYP2E1 expression and contributes to enhancing alcohol-induced liver injury. Retinoic acid-related orphan receptor alpha (RORα) has ...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkv1034
更新日期:2016-02-18 00:00:00
abstract::A soluble enzyme system that specifically initiates lambda dv plasmid DNA replication at a bacteriophage lambda replication origin [Wold et al. (1982) Proc. Natl. Acad. Sci. USA 79, 6176-6180] is also capable of replicating the single-stranded circular chromosomes of phages M13 and phi X174 to a duplex form. This chai...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/12.7.3069
更新日期:1984-04-11 00:00:00
abstract::Differential scanning microcalorimetry (DSC), UV absorption and circular dichroism (CD) have been used to study structure and stability of linear (lin), open circular (oc), supercoiled (cd) and relaxed circular duplex (rd) DNA and calf thymus (CT) DNA. Investigations were made in low salt buffer and in the presence of...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/16.24.11737
更新日期:1988-12-23 00:00:00
abstract::Three clones of non-repetitive sequences and six clones containing repetitive sequences were obtained from micronuclear DNA of Tetrahymena thermophila. All the non-repetitive and three repetitive sequences had the same organization in micro- and macronuclear DNAs as revealed by blot hybridization. On the other hand, t...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/10.14.4279
更新日期:1982-07-24 00:00:00
abstract::DNA polymerase lambda (pol lambda) is a member of the X family DNA polymerases and is endowed with multiple enzymatic activities. In this work we investigated the in vitro miscoding properties of full-length, human pol lambda either in the absence or in the presence of the human auxiliary proteins proliferating cell n...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkl032
更新日期:2006-03-06 00:00:00
abstract::RNA thermometers control the translation of several heat shock and virulence genes by their temperature-sensitive structural transitions. Changes in the structure and dynamics of MiniROSE RNA, which regulates translation in the temperature range of 20-45°C, were studied by site specifically replacing seven adenine res...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gku1264
更新日期:2015-01-01 00:00:00
abstract::Scalable multiplexed amplification technologies are needed for cost-effective large-scale genotyping of genetic markers such as single nucleotide polymorphisms (SNPs). We present SNPWave, a novel SNP genotyping technology to detect various subsets of sequences in a flexible fashion in a fixed detection format. SNPWave...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gnh045
更新日期:2004-03-05 00:00:00
abstract::Analysis of processing, assembly, and function of higher eukaryotic ribosomal RNA (rRNA) has been hindered by the lack of an expression system that enables rRNA to be modified and then examined functionally. Given the potential usefulness of such a system, we have developed one for mammalian 18S rRNA. We inserted a se...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gks530
更新日期:2012-09-01 00:00:00
abstract::Studying the intricate folding of rRNA within the ribosome remains a complex problem. Phenanthroline-Cu(II) complexes cleave phosphodiester bonds in rRNA in specific regions, apparently especially where the rRNA structure is constrained in some fashion. We have introduced phenanthroline-copper complexes into 50S Esche...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/27.8.1906
更新日期:1999-04-15 00:00:00
abstract::The J-binding protein 1 (JBP1) is essential for biosynthesis and maintenance of DNA base-J (β-d-glucosyl-hydroxymethyluracil). Base-J and JBP1 are confined to some pathogenic protozoa and are absent from higher eukaryotes, prokaryotes and viruses. We show that JBP1 recognizes J-containing DNA (J-DNA) through a 160-res...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkr125
更新日期:2011-07-01 00:00:00
abstract::A dot hybridization method is presented for rapidly determining the relative concentrations of nucleic acids in a mixture, as well as the extent of sequence homology between related RNA or DNA species. ...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/7.6.1541
更新日期:1979-11-24 00:00:00
abstract::The hexamer (m5 dC-dG)3 has been synthesized and its three-dimensional structure determined by a single crystal X-ray diffraction analysis. The structure has been refined to a final R value of 15.6% at 1.3 A resolution. The molecule forms a left-handed Z-DNA helix which is similar to the unmethylated Z-DNA structure. ...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/10.23.7879
更新日期:1982-12-11 00:00:00
abstract::A new method is described for the direct construction of randomly mutagenized genes by applying the polymerase chain reaction (PCR) to an oligonucleotide synthesized using doped nucleotide reservoirs. We have demonstrated the utility of this method by generating a library of mutant HIV-1 tat genes. Several arbitrarily...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/20.20.5311
更新日期:1992-10-25 00:00:00
abstract::This review presents detailed information about the structure of triplet repeat RNA and addresses the simple sequence repeats of normal and expanded lengths in the context of the physiological and pathogenic roles played in human cells. First, we discuss the occurrence and frequency of various trinucleotide repeats in...
journal_title:Nucleic acids research
pub_type: 杂志文章,评审
doi:10.1093/nar/gkr729
更新日期:2012-01-01 00:00:00
abstract::Biological networks play increasingly important roles in omics data integration and systems biology. Over the past decade, many excellent tools have been developed to support creation, analysis and visualization of biological networks. However, important limitations remain: most tools are standalone programs, the majo...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gky510
更新日期:2018-07-02 00:00:00
abstract::Replication Protein A (RPA) is a critical complex that acts in replication and promotes homologous recombination by allowing recombinase recruitment to processed DSB ends. Most organisms possess three RPA subunits (RPA1, RPA2, RPA3) that form a trimeric complex critical for viability. The Caenorhabditis elegans genome...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkaa1293
更新日期:2021-01-21 00:00:00
abstract::Numerous RNA-binding proteins have modular structures, comprising one or several copies of a selective RNA-binding domain generally coupled to an auxiliary domain that binds RNA non-specifically. We have built and compared homology-based models of the cold-shock domain (CSD) of the Xenopus protein, FRGY2, and of the t...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/29.11.2223
更新日期:2001-06-01 00:00:00
abstract::CS23D (chemical shift to 3D structure) is a web server for rapidly generating accurate 3D protein structures using only assigned nuclear magnetic resonance (NMR) chemical shifts and sequence data as input. Unlike conventional NMR methods, CS23D requires no NOE and/or J-coupling data to perform its calculations. CS23D ...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkn305
更新日期:2008-07-01 00:00:00
abstract::Chromatin core particles near physiological ionic strength undergo a reversible transition induced by changes in pH near neutrality. While sedimentation studies indicate no significant effect on size or shape, changes in tyrosine fluorescence anisotropy and in circular dichroism suggest a somewhat looser structure at ...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/12.10.4351
更新日期:1984-05-25 00:00:00
abstract::Rapid and transient activation of both c-fos transcription and mRNA accumulation occurs when resting CCL39 hamster fibroblasts are serum-stimulated to grow. By using several combinations of serum and cycloheximide, a protein synthesis inhibitor, we showed that: i) addition of cycloheximide to resting cell elicits an i...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/15.14.5657
更新日期:1987-07-24 00:00:00
abstract::We have investigated the substrate specificity of the Ogg1 protein of Saccharomyces cerevisiae (yOgg1 protein) for excision of modified DNA bases from oxidatively damaged DNA substrates using gas chromatography/isotope dilution mass spectrometry. Four DNA substrates prepared by treatment with H2O2/Fe(III)-EDTA/ascorbi...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/26.5.1228
更新日期:1998-03-01 00:00:00
abstract::Clustered damages are formed in DNA by ionising radiation and radiomimetic anticancer agents and are thought to be biologically severe. 7,8-dihydro-8-oxoguanine (8-oxoG), a major DNA damage resulting from oxidative attack, is highly mutagenic leading to a high level of G.C-->T.A transversions if not previously excised...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/29.5.1107
更新日期:2001-03-01 00:00:00
abstract::Major efforts are underway to elucidate the spatial distribution of ribosomal RNAs (rRNAs) in the ribosome. An especially informative approach is the identification of likely base-base tertiary interactions within the RNA by phylogenetic comparison. Herein evidence is presented for three heretofore unrecognized candid...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/17.6.2215
更新日期:1989-03-25 00:00:00