Abstract:
:DNA polymerase lambda (pol lambda) is a member of the X family DNA polymerases and is endowed with multiple enzymatic activities. In this work we investigated the in vitro miscoding properties of full-length, human pol lambda either in the absence or in the presence of the human auxiliary proteins proliferating cell nuclear antigen (PCNA) and replication protein A (RP-A). Our data suggested that (i) pol lambda had an intrinsic ability to create mismatches and to incorporate ribonucleotides at nearly physiological Mn++ and Mg++ concentrations; (ii) the sequence of the template-primer could influence the misincorporation frequency of pol lambda; (iii) pol lambda preferentially generated G:T and G:G mismatches; (iv) RP-A, but not PCNA, selectively prevented misincorporation of an incorrect nucleotide by pol lambda, without affecting correct incorporation and (v) this inhibitory effect required a precise ratio between the concentrations of pol lambda and RP-A. Possible physiological implications of these findings for the in vivo fidelity of pol lambda are discussed.
journal_name
Nucleic Acids Resjournal_title
Nucleic acids researchauthors
Maga G,Shevelev I,Villani G,Spadari S,Hübscher Udoi
10.1093/nar/gkl032keywords:
subject
Has Abstractpub_date
2006-03-06 00:00:00pages
1405-15issue
5eissn
0305-1048issn
1362-4962pii
34/5/1405journal_volume
34pub_type
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