当前位置： SCI文献检索 > NUCLEIC ACIDS RESEARCH期刊下所有文献 > Ubiquitous expression of the 43- and 44-kDa forms of transcription factor USF in mammalian cells.

Ubiquitous expression of the 43- and 44-kDa forms of transcription factor USF in mammalian cells.

Abstract：

:USF is a helix-loop-helix transcription factor that, like Myc, recognizes the DNA binding motif CACGTG. Two different forms of USF, characterized by apparent molecular weights of 43,000 and 44,000, were originally identified in HeLa cells by biochemical analysis. Clones for the 43-kDa USF were first characterized, but only partial clones for the human 44-kDa USF (USF2, or FIP) have been reported. Here we describe a complete cDNA for the 44-kDa USF from murine cells. Analysis of this clone has revealed that the various USF family members are quite divergent in their N-terminal amino acid sequences, while a high degree of conservation characterizes their dimerization and DNA-binding domains. Interestingly, the 3' noncoding region of the 44-kDa USF cDNAs displayed an unusual degree of conservation between human and mouse. In vitro transcription/translation experiments indicated a possible role for this region in translation regulation. Alternative splicing forms of the 44-kDa USF messages exist in both mouse and human. Examination of the tissue and cell-type distribution of USF by Northern blot and gel retardation assays revealed that while expression of both the 43- and 44-kDa USF species is ubiquitous, different ratios of USF homo- and heterodimers are found in different cells.

journal_name

Nucleic Acids Res

journal_title

Nucleic acids research

authors

Sirito M,Lin Q,Maity T,Sawadogo M

doi

10.1093/nar/22.3.427

subject

Has Abstract

pub_date

1994-02-11 00:00:00

pages

427-33

issue

3

eissn

0305-1048

issn

1362-4962

journal_volume

22

pub_type

杂志文章

相关文献

NUCLEIC ACIDS RESEARCH文献大全
  • Biochemical and electron microscope analyses of the DNA reverse transcripts present in the virus-like particles of the yeast transposon Ty1. Identification of a second origin of Ty1DNA plus strand synthesis.

    abstract：:Transposition of Saccharomyces cerevisiae Ty1 retroelements has been shown to involve reverse transcription in intracytoplasmic virus-like particles (Ty-VLPs). Ty DNA present in the particles specified by Ty1-H3 element was found to consist of the full-length genomic DNA as well as incomplete cDNAs mainly of plus pola...

    journal_title：Nucleic acids research

    pub_type： 杂志文章

    doi：10.1093/nar/21.15.3513

    authors： Pochart P,Agoutin B,Rousset S,Chanet R,Doroszkiewicz V,Heyman T

    更新日期：1993-07-25 00:00:00

  • High level activity of the mouse CCAAT/enhancer binding protein (C/EBP alpha) gene promoter involves autoregulation and several ubiquitous transcription factors.

    abstract：:The promoter region of the mouse CCAAT-Enhancer Binding Protein (C/EBP alpha) gene is capable of directing high levels of expression of reporter constructs in various cell lines, albeit even in cells that do not express their endogenous C/EBP alpha gene. To understand the molecular mechanisms underlying this ubiquitou...

    journal_title：Nucleic acids research

    pub_type： 杂志文章

    doi：10.1093/nar/21.8.1735

    authors： Legraverend C,Antonson P,Flodby P,Xanthopoulos KG

    更新日期：1993-04-25 00:00:00

  • TIGRFAMs and Genome Properties: tools for the assignment of molecular function and biological process in prokaryotic genomes.

    abstract：:TIGRFAMs is a collection of protein family definitions built to aid in high-throughput annotation of specific protein functions. Each family is based on a hidden Markov model (HMM), where both cutoff scores and membership in the seed alignment are chosen so that the HMMs can classify numerous proteins according to the...

    journal_title：Nucleic acids research

    pub_type： 杂志文章

    doi：10.1093/nar/gkl1043

    authors： Selengut JD,Haft DH,Davidsen T,Ganapathy A,Gwinn-Giglio M,Nelson WC,Richter AR,White O

    更新日期：2007-01-01 00:00:00

  • A coronary artery disease-associated tRNAThr mutation altered mitochondrial function, apoptosis and angiogenesis.

    abstract：:The tissue specificity of mitochondrial tRNA mutations remains largely elusive. In this study, we demonstrated the deleterious effects of tRNAThr 15927G>A mutation that contributed to pathogenesis of coronary artery disease. The m.15927G>A mutation abolished the highly conserved base-pairing (28C-42G) of anticodon ste...

    journal_title：Nucleic acids research

    pub_type： 杂志文章

    doi：10.1093/nar/gky1241

    authors： Jia Z,Zhang Y,Li Q,Ye Z,Liu Y,Fu C,Cang X,Wang M,Guan MX

    更新日期：2019-02-28 00:00:00

  • The repeating nucleotide sequence in the repetitive mitochondrial DNA from a "low-density" petite mutant of yeast.

    abstract：:The repeating nucleotide sequence of 68 base pairs in the mtDNA from an ethidium-induced cytoplasmic petite mutant of yeast has been determined. For sequence analysis specifically primed and terminated RNA copies, obtained by in vitro transcription of the separated strands, were use. The sequence consists of 66 consec...

    journal_title：Nucleic acids research

    pub_type： 杂志文章

    doi：10.1093/nar/4.7.2369

    authors： Van Kreijl CF,Bos JL

    更新日期：1977-07-01 00:00:00

  • In vitro site-directed mutagenesis with synthetic DNA oligonucleotides yields unexpected deletions and insertions at high frequency.

    abstract：:We have used in vitro site-directed mutagenesis with synthetic DNA oligonucleotides to introduce single nucleotide mutations in yeast mtDNA. In addition to the expected DNA alterations we also recovered with high frequency mutants with large deletions and insertions which arose through interaction with the synthetic D...

    journal_title：Nucleic acids research

    pub_type： 杂志文章

    doi：10.1093/nar/11.24.8595

    authors： Osinga KA,Van der Bliek AM,Van der Horst G,Groot Koerkamp MJ,Tabak HF,Veeneman GH,Van Boom JH

    更新日期：1983-12-20 00:00:00

  • PLAST-ncRNA: Partition function Local Alignment Search Tool for non-coding RNA sequences.

    abstract：:Alignment-based programs are valuable tools for finding potential homologs in genome sequences. Previously, it has been shown that partition function posterior probabilities attuned to local alignment achieve a high accuracy in identifying distantly similar non-coding RNA sequences that are hidden in a large genome. H...

    journal_title：Nucleic acids research

    pub_type： 杂志文章

    doi：10.1093/nar/gkq487

    authors： Chikkagoudar S,Livesay DR,Roshan U

    更新日期：2010-07-01 00:00:00

  • MetaNetX/MNXref--reconciliation of metabolites and biochemical reactions to bring together genome-scale metabolic networks.

    abstract：:MetaNetX is a repository of genome-scale metabolic networks (GSMNs) and biochemical pathways from a number of major resources imported into a common namespace of chemical compounds, reactions, cellular compartments--namely MNXref--and proteins. The MetaNetX.org website (http://www.metanetx.org/) provides access to the...

    journal_title：Nucleic acids research

    pub_type： 杂志文章

    doi：10.1093/nar/gkv1117

    authors： Moretti S,Martin O,Van Du Tran T,Bridge A,Morgat A,Pagni M

    更新日期：2016-01-04 00:00:00

  • Temperature-sensitive mutation in yeast mitochondrial ribosome recycling factor (RRF).

    abstract：:The yeast protein Rrf1p encoded by the FIL1 nuclear gene bears significant sequence similarity to Escherichia coli ribosome recycling factor (RRF). Here, we call FIL1 Ribosome Recycling Factor of yeast, RRF1. Its gene product, Rrf1p, was localized in mitochondria. Deletion of RRF1 leads to a respiratory incompetent ph...

    journal_title：Nucleic acids research

    pub_type： 杂志文章

    doi：10.1093/nar/gkg449

    authors： Teyssier E,Hirokawa G,Tretiakova A,Jameson B,Kaji A,Kaji H

    更新日期：2003-07-15 00:00:00

  • Partial purification and characterization of two cytoplasmic DNA polymerases from ungerminated wheat.

    abstract：:Two DNA polymerases have been purified from the 105,000 x g supernatant of ungerminated wheat. The purification stages included: high speed centrifugation, salt fractionation, DEAE-cellulose chromatography, Sephadex G-150 filtration and phosphocellulose chromatography. Several properties of the two enzyme (called A an...

    journal_title：Nucleic acids research

    pub_type： 杂志文章

    doi：10.1093/nar/2.11.2077

    authors： Castroviejo M,Tarragó-Litvak L,Litvak S

    更新日期：1975-11-01 00:00:00

  • The UniTrap resource: tools for the biologist enabling optimized use of gene trap clones.

    abstract：:We have developed a comprehensive resource devoted to biologists wanting to optimize the use of gene trap clones in their experiments. We have processed 300 602 such clones from both public and private projects to generate 28,199 'UniTraps', i.e. distinct collections of unambiguous insertions at the same subgenic regi...

    journal_title：Nucleic acids research

    pub_type： 杂志文章

    doi：10.1093/nar/gkm825

    authors： Roma G,Sardiello M,Cobellis G,Cruz P,Lago G,Sanges R,Stupka E

    更新日期：2008-01-01 00:00:00

  • PASMet: a web-based platform for prediction, modelling and analyses of metabolic systems.

    abstract：:PASMet (Prediction, Analysis and Simulation of Metabolic networks) is a web-based platform for proposing and verifying mathematical models to understand the dynamics of metabolism. The advantages of PASMet include user-friendliness and accessibility, which enable biologists and biochemists to easily perform mathematic...

    journal_title：Nucleic acids research

    pub_type： 杂志文章

    doi：10.1093/nar/gkw415

    authors： Sriyudthsak K,Mejia RF,Arita M,Hirai MY

    更新日期：2016-07-08 00:00:00

  • Germline ablation of SMUG1 DNA glycosylase causes loss of 5-hydroxymethyluracil- and UNG-backup uracil-excision activities and increases cancer predisposition of Ung-/-Msh2-/- mice.

    abstract：:Deamination of cytosine (C), 5-methylcytosine (mC) and 5-hydroxymethylcytosine (hmC) occurs spontaneously in mammalian DNA with several hundred deaminations occurring in each cell every day. The resulting potentially mutagenic mispairs of uracil (U), thymine (T) or 5-hydroxymethyluracil (hmU) with guanine (G) are subs...

    journal_title：Nucleic acids research

    pub_type： 杂志文章

    doi：10.1093/nar/gks259

    authors： Kemmerich K,Dingler FA,Rada C,Neuberger MS

    更新日期：2012-07-01 00:00:00

  • Multi-omic analysis of gametogenesis reveals a novel signature at the promoters and distal enhancers of active genes.

    abstract：:Epigenetic regulation of gene expression is tightly controlled by the dynamic modification of histones by chemical groups, the diversity of which has largely expanded over the past decade with the discovery of lysine acylations, catalyzed from acyl-coenzymes A. We investigated the dynamics of lysine acetylation and cr...

    journal_title：Nucleic acids research

    pub_type： 杂志文章

    doi：10.1093/nar/gkaa163

    authors： Crespo M,Damont A,Blanco M,Lastrucci E,Kennani SE,Ialy-Radio C,Khattabi LE,Terrier S,Louwagie M,Kieffer-Jaquinod S,Hesse AM,Bruley C,Chantalat S,Govin J,Fenaille F,Battail C,Cocquet J,Pflieger D

    更新日期：2020-05-07 00:00:00

  • Purification by DNA affinity precipitation of the cellular factors HEB1-p67 and HEB1-p94 which bind specifically to the human T-cell leukemia virus type-I 21 bp enhancer.

    abstract：:Transcription driven by the proviral promoter of the Human T-cell Leukemia Virus type I (HTLV-I) is tightly regulated by the Tax1 transactivator. This viral protein potently induces the enhancer activity of a 21 bp motif repeated three times in the promoter. We have previously shown that this induction results from th...

    journal_title：Nucleic acids research

    pub_type： 杂志文章

    doi：10.1093/nar/21.17.3935

    authors： Lombard-Platet G,Jalinot P

    更新日期：1993-08-25 00:00:00

  • Models of triple-stranded polynucleotides with optimised stereochemistry.

    abstract：:Detailed models are presented for the triple-stranded polynucleotide helices of poly (U)-poly (A)-poly (U) (two forms), poly (U)-poly d (A) -poly (U), poly d(C)-poly d(I)-poly d(C), poly d(T)-polyd(A)-poly d(T) and poly (I)-poly (A)-poly (I). The models were genrated using a computerized, linked-atom procedure which p...

    journal_title：Nucleic acids research

    pub_type： 杂志文章

    doi：10.1093/nar/3.10.2459

    authors： Arnott S,Bond PJ,Selsing E,Smith PJ

    更新日期：1976-10-01 00:00:00

  • High affinity nucleic acid aptamers for streptavidin incorporated into bi-specific capture ligands.

    abstract：:We have isolated 2'-Fluoro-substituted RNA aptamers that bind to streptavidin (SA) with an affinity around 7 +/- 1.8 nM, comparable with that of recently described peptide aptamers. Binding to SA was not prevented by prior saturation with biotin, enabling nucleic acid aptamers to form useful ternary complexes. Mutagen...

    journal_title：Nucleic acids research

    pub_type： 杂志文章

    doi：10.1093/nar/30.10.e45

    authors： Tahiri-Alaoui A,Frigotto L,Manville N,Ibrahim J,Romby P,James W

    更新日期：2002-05-15 00:00:00

  • The evolutionary scrambling and developmental unscrambling of germline genes in hypotrichous ciliates.

    abstract：:Genes in the germline (micronuclear) genome of hypotrichous ciliates are interrupted by multiple, short, non-coding, AT-rich sequences called internal eliminated segments, or IESs. During conversion of a micronucleus to a somatic nucleus (macronucleus) after cell mating, all IESs are excised from the germline genes an...

    journal_title：Nucleic acids research

    pub_type： 杂志文章,评审

    doi：10.1093/nar/27.5.1243

    authors： Prescott DM

    更新日期：1999-03-01 00:00:00

  • OpenTein: a database of digital whole-slide images of stem cell-derived teratomas.

    abstract：:Human stem cells are promising sources for regenerative therapy. To ensure safety of future therapeutic applications, the differentiation potency of stem cells has to be tested and be widely opened to the public. The potency is generally assessed by teratoma formation comprising differentiated cells from all three ger...

    journal_title：Nucleic acids research

    pub_type： 杂志文章

    doi：10.1093/nar/gkv1096

    authors： Park SJ,Komiyama Y,Suemori H,Umezawa A,Nakai K

    更新日期：2016-01-04 00:00:00

  • Structural organization of mRNA complexes with major core mRNP protein YB-1.

    abstract：:YB-1 is a universal major protein of cytoplasmic mRNPs, a member of the family of multifunctional cold shock domain proteins (CSD proteins). Depending on its amount on mRNA, YB-1 stimulates or inhibits mRNA translation. In this study, we have analyzed complexes formed in vitro at various YB-1 to mRNA ratios, including...

    journal_title：Nucleic acids research

    pub_type： 杂志文章

    doi：10.1093/nar/gkh889

    authors： Skabkin MA,Kiselyova OI,Chernov KG,Sorokin AV,Dubrovin EV,Yaminsky IV,Vasiliev VD,Ovchinnikov LP

    更新日期：2004-10-19 00:00:00

  • Smarcal1 promotes double-strand-break repair by nonhomologous end-joining.

    abstract：:Smarcal1 is a SWI/SNF-family protein with an ATPase domain involved in DNA-annealing activities and a binding site for the RPA single-strand-DNA-binding protein. Although the role played by Smarcal1 in the maintenance of replication forks has been established, it remains unknown whether Smarcal1 contributes to genomic...

    journal_title：Nucleic acids research

    pub_type： 杂志文章

    doi：10.1093/nar/gkv621

    authors： Keka IS,Mohiuddin,Maede Y,Rahman MM,Sakuma T,Honma M,Yamamoto T,Takeda S,Sasanuma H

    更新日期：2015-07-27 00:00:00

  • BioModels Database: a free, centralized database of curated, published, quantitative kinetic models of biochemical and cellular systems.

    abstract：:BioModels Database (http://www.ebi.ac.uk/biomodels/), part of the international initiative BioModels.net, provides access to published, peer-reviewed, quantitative models of biochemical and cellular systems. Each model is carefully curated to verify that it corresponds to the reference publication and gives the proper...

    journal_title：Nucleic acids research

    pub_type： 杂志文章

    doi：10.1093/nar/gkj092

    authors： Le Novère N,Bornstein B,Broicher A,Courtot M,Donizelli M,Dharuri H,Li L,Sauro H,Schilstra M,Shapiro B,Snoep JL,Hucka M

    更新日期：2006-01-01 00:00:00

  • The Reactome pathway knowledgebase.

    abstract：:Reactome (http://www.reactome.org) is a manually curated open-source open-data resource of human pathways and reactions. The current version 46 describes 7088 human proteins (34% of the predicted human proteome), participating in 6744 reactions based on data extracted from 15 107 research publications with PubMed link...

    journal_title：Nucleic acids research

    pub_type： 杂志文章

    doi：10.1093/nar/gkt1102

    authors： Croft D,Mundo AF,Haw R,Milacic M,Weiser J,Wu G,Caudy M,Garapati P,Gillespie M,Kamdar MR,Jassal B,Jupe S,Matthews L,May B,Palatnik S,Rothfels K,Shamovsky V,Song H,Williams M,Birney E,Hermjakob H,Stein L,D'Eusta

    更新日期：2014-01-01 00:00:00

  • Human telomere, oncogenic promoter and 5'-UTR G-quadruplexes: diverse higher order DNA and RNA targets for cancer therapeutics.

    abstract：:Guanine-rich DNA sequences can form G-quadruplexes stabilized by stacked G-G-G-G tetrads in monovalent cation-containing solution. The length and number of individual G-tracts and the length and sequence context of linker residues define the diverse topologies adopted by G-quadruplexes. The review highlights recent so...

    journal_title：Nucleic acids research

    pub_type： 杂志文章,评审

    doi：10.1093/nar/gkm711

    authors： Patel DJ,Phan AT,Kuryavyi V

    更新日期：2007-01-01 00:00:00

  • Uncoupling of SV40 tsA replicon activation from DNA chain elongation by temperature shifts and aphidicolin arrest.

    abstract：:To synchronize SV40 replicons, simian cells infected with a tsA mutant were restricted at 40 degrees, to complete ongoing replication and returned to 32 degrees, to activate new replicons in the presence of the DNA chain elongation inhibitor aphidicolin. Upon further incubation at 40 degrees without the drug, 3H-dT wa...

    journal_title：Nucleic acids research

    pub_type： 杂志文章

    doi：10.1093/nar/10.2.763

    authors： Dinter-Gottlieb G,Kaufmann G

    更新日期：1982-01-22 00:00:00

  • A basal level of DNA damage and telomere deprotection increases the sensitivity of cancer cells to G-quadruplex interactive compounds.

    abstract：:Here, with the aim of obtaining insight into the intriguing selectivity of G-quadruplex (G4) ligands toward cancer compared to normal cells, a genetically controlled system of progressive transformation in human BJ fibroblasts was analyzed. Among the different comparative evaluations, we found a progressive increase o...

    journal_title：Nucleic acids research

    pub_type： 杂志文章

    doi：10.1093/nar/gkv006

    authors： Salvati E,Rizzo A,Iachettini S,Zizza P,Cingolani C,D'Angelo C,Porru M,Mondello C,Aiello A,Farsetti A,Gilson E,Leonetti C,Biroccio A

    更新日期：2015-02-18 00:00:00

  • A novel nuclear inhibitor I-92 regulates DNA binding activity of octamer binding protein p92 during the cell cycle.

    abstract：:Nuclear DNA binding protein p92 is a sequence specific octamer binding protein with identical molecular weight as the ubiquitous octamer binding protein Oct-1. It binds to octamer related sequences from the enhancer of human papillomavirus type 18. The activity and intracellular distribution of p92 is regulated by ext...

    journal_title：Nucleic acids research

    pub_type： 杂志文章

    doi：10.1093/nar/19.20.5725

    authors： Weitz J,Kopun M,Stoehr M,Napierski I,Royer HD

    更新日期：1991-10-25 00:00:00

  • RegAnalyst: a web interface for the analysis of regulatory motifs, networks and pathways.

    abstract：:RegAnalyst is a user-friendly web interface that integrates MoPP (Motif Prediction Program), MyPatternFinder (pattern detection tool) and MycoRegDB (mycobacterial promoter and regulatory elements database). Since motif discovery is a challenging task, numerous tools have been developed over the past few years. Strikin...

    journal_title：Nucleic acids research

    pub_type： 杂志文章

    doi：10.1093/nar/gkp388

    authors： Sharma D,Mohanty D,Surolia A

    更新日期：2009-07-01 00:00:00

  • Effects of pH on the stability of chromatin core particles.

    abstract：:Chromatin core particles near physiological ionic strength undergo a reversible transition induced by changes in pH near neutrality. While sedimentation studies indicate no significant effect on size or shape, changes in tyrosine fluorescence anisotropy and in circular dichroism suggest a somewhat looser structure at ...

    journal_title：Nucleic acids research

    pub_type： 杂志文章

    doi：10.1093/nar/12.10.4351

    authors： Libertini LJ,Small EW

    更新日期：1984-05-25 00:00:00

  • Proteotoxic stress promotes entrapment of ribosomes and misfolded proteins in a shared cytosolic compartment.

    abstract：:Cells continuously monitor protein synthesis to prevent accumulation of aberrant polypeptides. Insufficient capacity of cellular degradative systems, chaperone shortage or high levels of mistranslation by ribosomes can result in proteotoxic stress and endanger proteostasis. One of the least explored reasons for mistra...

    journal_title：Nucleic acids research

    pub_type： 杂志文章

    doi：10.1093/nar/gkaa068

    authors： Ghosh A,Williams LD,Pestov DG,Shcherbik N

    更新日期：2020-04-17 00:00:00