Abstract:
:The yeast protein Rrf1p encoded by the FIL1 nuclear gene bears significant sequence similarity to Escherichia coli ribosome recycling factor (RRF). Here, we call FIL1 Ribosome Recycling Factor of yeast, RRF1. Its gene product, Rrf1p, was localized in mitochondria. Deletion of RRF1 leads to a respiratory incompetent phenotype and to instability of the mitochondrial genome (conversion to rho(-)/rho(0) cytoplasmic petites). Yeast with intact mitochondria and with deleted genomic RRF1 that harbors a plasmid carrying RRF1 was prepared from spores of heterozygous diploid yeast. Such yeast with a mutated allele of RRF1, rrf1-L209P, grew on a non-fermentable carbon source at 30 but not at 36 degrees C, where mitochondrial but not total protein synthesis was 90% inhibited. We propose that Rrf1p is essential for mitochondrial protein synthesis and acts as a RRF in mitochondria.
journal_name
Nucleic Acids Resjournal_title
Nucleic acids researchauthors
Teyssier E,Hirokawa G,Tretiakova A,Jameson B,Kaji A,Kaji Hdoi
10.1093/nar/gkg449keywords:
subject
Has Abstractpub_date
2003-07-15 00:00:00pages
4218-26issue
14eissn
0305-1048issn
1362-4962journal_volume
31pub_type
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