Genetic organization of alpha-bungarotoxins from Bungarus multicinctus (Taiwan banded krait): evidence showing that the production of alpha-bungarotoxin isotoxins is not derived from edited mRNAs.

abstract：:DNA adenine methyltransferase (Dam) is widespread and conserved among the γ-proteobacteria. Methylation of the Ade in GATC sequences regulates diverse bacterial cell functions, including gene expression, mismatch repair and chromosome replication. Dam also controls virulence in many pathogenic Gram-negative bacteria. ...

journal_title：Nucleic acids research

authors： Horton JR,Zhang X,Blumenthal RM,Cheng X

更新日期：2015-04-30 00:00:00

abstract：:We report the isolation of a novel human POU domain encoding gene named RDC-1. The POU domain of the RDC-1 encoded protein is highly related to the POU domain potentially encoded by the rat brain-3 sequence and to that of the Drosophila I-POU protein; outside of the POU region, RDC-1 is unrelated to any previously cha...

journal_title：Nucleic acids research

authors： Collum RG,Fisher PE,Datta M,Mellis S,Thiele C,Huebner K,Croce CM,Israel MA,Theil T,Moroy T

更新日期：1992-09-25 00:00:00

abstract：:Infrared spectroscopy was used to identify hydration-sensitive structural differences between single- stranded phosphorothioate (PS) and phosphodiester (PO) oligodeoxyribonucleotides. Spectra were recorded in the mid-infrared region, 500-1800 cm-1, at relative humidities between 0 and 98%; the PS and PO spectra are su...

journal_title：Nucleic acids research

authors： White AP,Reeves KK,Snyder E,Farrell J,Powell JW,Mohan V,Griffey RH

更新日期：1996-08-15 00:00:00

abstract：:Accurate T-cell epitope prediction is a principal objective of computational vaccinology. As a service to the immunology and vaccinology communities at large, we have implemented, as a server on the World Wide Web, a partial least squares-based multivariate statistical approach to the quantitative prediction of peptid...

journal_title：Nucleic acids research

authors： Guan P,Doytchinova IA,Zygouri C,Flower DR

更新日期：2003-07-01 00:00:00

abstract：:Topoisomerases (topos) maintain DNA topology and influence DNA transaction processes by catalysing relaxation, supercoiling and decatenation reactions. In the cellular milieu, division of labour between different topos ensures topological homeostasis and control of central processes. In Escherichia coli, DNA gyrase is...

journal_title：Nucleic acids research

authors： Kumar R,Riley JE,Parry D,Bates AD,Nagaraja V

更新日期：2012-11-01 00:00:00

abstract：:'Linker scanning' has been used as an efficient method for systematically surveying a segment of DNA for functional elements by mutagenesis. A three-step PCR method was developed to simplify this process. In this method, a set of 'mutation primers' was made with 6 to 8 base substitutions in the center of the primers. ...

journal_title：Nucleic acids research

authors： Li XM,Shapiro LJ

更新日期：1993-08-11 00:00:00

abstract：:In Xenopus oocytes in vitro transcribed mouse U7 RNA is assembled into small nuclear ribonucleoproteins (snRNPs) that are functional in histone RNA 3' processing. If the special Sm binding site of U7 (AAUUUGUCUAG, U7 Sm WT) is converted into the canonical Sm sequence derived from the major snRNAs (AAUUUUUGGAG, U7 Sm O...

journal_title：Nucleic acids research

authors： Stefanovic B,Hackl W,Lührmann R,Schümperli D

更新日期：1995-08-25 00:00:00

abstract：:Ribonucleic acids are highly conserved essential parts of cellular life. RNA function is determined to a large extent by its hydrodynamic behaviour. The presented study proposes a strategy to predict the hydrodynamic behaviour of RNA single strands on the basis of the polymer size. By atom-level shell-modelling of hig...

journal_title：Nucleic acids research

authors： Werner A

更新日期：2011-02-01 00:00:00

abstract：:EcoGene (http://ecogene.org) is a database and website devoted to continuously improving the structural and functional annotation of Escherichia coli K-12, one of the most well understood model organisms, represented by the MG1655(Seq) genome sequence and annotations. Major improvements to EcoGene in the past decade i...

journal_title：Nucleic acids research

authors： Zhou J,Rudd KE

更新日期：2013-01-01 00:00:00

abstract：:Signal recognition particle (SRP) is a stable cytoplasmic ribonucleoprotein complex that serves to translocate secretory proteins across membranes during translation. The SRP Database (SRPDB) provides compilations of SRP components, ordered alphabetically and phylogenetically. Alignments emphasize phylogenetically-sup...

journal_title：Nucleic acids research

authors： Gorodkin J,Knudsen B,Zwieb C,Samuelsson T

更新日期：2001-01-01 00:00:00

abstract：:We characterised the human hSuv3p protein belonging to the family of NTPases/helicases. In yeast mitochondria the hSUV3 orthologue is a component of the degradosome complex and participates in mtRNA turnover and processing, while in Caenorhabditis elegans the hSUV3 orthologue is necessary for viability of early embryo...

journal_title：Nucleic acids research

authors： Minczuk M,Piwowarski J,Papworth MA,Awiszus K,Schalinski S,Dziembowski A,Dmochowska A,Bartnik E,Tokatlidis K,Stepien PP,Borowski P

更新日期：2002-12-01 00:00:00

abstract：:Dinucleoside diphosphates of the general type pGpN have been prepared enzymatically using ribonuclease N1. Alkylated uridines or cytidines, which are products of carcinogens acting on nucleic acids, were tested in dinucleoside diphosphates for their ability to stimulate the binding of Ala- or Val-tRNA to ribosomes. O2...

journal_title：Nucleic acids research

authors： Singer B,Pergolizzi RG,Grunberger D

更新日期：1979-04-01 00:00:00

abstract：:Fourteen P-element insertion mutants at the yellow (y) gene of D. melanogaster have been analysed by restriction mapping and DNA sequencing. The distribution of the insertion sites is nonrandom, with a preferred target site in the 5'-transcribed but non-translated leader of the gene. Transcription analysis indicates t...

journal_title：Nucleic acids research

authors： Howes G,O'Connor M,Chia W

更新日期：1988-04-11 00:00:00

abstract：:Bisulfite genomic sequencing is the most widely used technique to analyze the 5-methylation of cytosines, the prevalent covalent DNA modification in mammals. The process is based on the selective transformation of unmethylated cytosines to uridines. Then, the investigated genomic regions are PCR amplified, subcloned a...

journal_title：Nucleic acids research

authors： Tusnády GE,Simon I,Váradi A,Arányi T

更新日期：2005-01-13 00:00:00

abstract：:The occurrence of DNA tracts of the three binary base combinations: R.Y, K.M and W;S has been mapped in the complete genomes of Haemophilus influenzae and Escherichia coli. A highly significant over-representation of W tracts is observed in both bacteria. The excess of W tracts is particularly striking in the 10% inte...

journal_title：Nucleic acids research

authors： Shomer B,Yagil G

更新日期：1999-11-15 00:00:00

abstract：:The double helix is known to form as a result of hybridization of complementary nucleic acid strands in aqueous solution. In the helix the negatively charged phosphate groups of each nucleic acid strand are distributed helically on the outside of the duplex and are available for interaction with cationic groups. Catio...

journal_title：Nucleic acids research

authors： Lemeshko SV,Powdrill T,Belosludtsev YY,Hogan M

更新日期：2001-07-15 00:00:00

abstract：:DNase I and MPE.Fe (II) footprinting both employ partial cleavage of ligand-protected DNA restriction fragments and Maxam-Gilbert sequencing gel methods of analysis. One method utilizes the enzyme, DNase I, as the DNA cleaving agent while the other employs the synthetic molecule, methidium-propyl-EDTA (MPE). For actin...

journal_title：Nucleic acids research

authors： Van Dyke MW,Dervan PB

更新日期：1983-08-25 00:00:00

abstract：:DIAL (dihedral alignment) is a web server that provides public access to a new dynamic programming algorithm for pairwise 3D structural alignment of RNA. DIAL achieves quadratic time by performing an alignment that accounts for (i) pseudo-dihedral and/or dihedral angle similarity, (ii) nucleotide sequence similarity a...

journal_title：Nucleic acids research

authors： Ferrè F,Ponty Y,Lorenz WA,Clote P

更新日期：2007-07-01 00:00:00

abstract：:Alfalfa mosaic virus RNA 4, the subgenomic messenger for viral coat protein, was partially digested with RNase T1 or RNase A and the sequence of a number of fragments was deduced by in vitro labeling with polynucleotide kinase and application of RNA sequencing techniques. From overlapping fragments, the complete prima...

journal_title：Nucleic acids research

authors： Brederode FT,Koper-Zwarthoff EC,Bol JF

更新日期：1980-05-24 00:00:00

abstract：:MicroRNA 26a (miR-26a) reduces cell viability in several cancers, indicating that miR-26a could be used as a therapeutic option in patients. We demonstrate that miR-26a not only inhibits G1-S cell cycle transition and promotes apoptosis, as previously described, but also regulates multiple cell cycle checkpoints. We s...

journal_title：Nucleic acids research

authors： Castellano L,Dabrowska A,Pellegrino L,Ottaviani S,Cathcart P,Frampton AE,Krell J,Stebbing J

更新日期：2017-05-05 00:00:00

abstract：:Poly(ADP-ribosyl)ation is involved in numerous bio-logical processes including DNA repair, transcription and cell death. Cellular levels of poly(ADP-ribose) (PAR) are regulated by PAR polymerases (PARPs) and the degrading enzyme PAR glycohydrolase (PARG), controlling the cell fate decision between life and death in re...

journal_title：Nucleic acids research

authors： Illuzzi G,Fouquerel E,Amé JC,Noll A,Rehmet K,Nasheuer HP,Dantzer F,Schreiber V

更新日期：2014-07-01 00:00:00

abstract：:Most RNA molecules collapse rapidly and reach the native state through a pathway that contains numerous traps and unproductive intermediates. The D135 group II intron ribozyme is unusual in that it can fold slowly and directly to the native state, despite its large size and structural complexity. Here we use hydroxyl ...

journal_title：Nucleic acids research

authors： Su LJ,Waldsich C,Pyle AM

更新日期：2005-11-27 00:00:00

abstract：:Cytosines in genomic DNA are sometimes methylated. This affects many biological processes and diseases. The standard way of measuring methylation is to use bisulfite, which converts unmethylated cytosines to thymines, then sequence the DNA and compare it to a reference genome sequence. We describe a method for the cri...

journal_title：Nucleic acids research

authors： Frith MC,Mori R,Asai K

更新日期：2012-07-01 00:00:00

abstract：:To elucidate the general constraints imposed on the structure of the D- and T-loops in functional tRNAs, active suppressor tRNAs were selected in vivo from a combinatorial tRNA gene library in which several nucleotide positions of these loops were randomized. Analysis of the nucleotide sequences of the selected clones...

journal_title：Nucleic acids research

authors： Zagryadskaya EI,Doyon FR,Steinberg SV

更新日期：2003-07-15 00:00:00

abstract：:A set of new sulfurizing agents representing disulfides of arylsulfonic acids has been developed for the automated synthesis of phosphorothioate oligonucleotide analogues via the phosphoramidite method. These reagents, such as bis(benzenesulfonyl)disulfide, bis(p-toluenesulfonyl)disulfide, bis(p-methoxybenzensulfonyl)...

journal_title：Nucleic acids research

authors： Efimov VA,Kalinkina AL,Chakhmakhcheva OG,Hill TS,Jayaraman K

更新日期：1995-10-25 00:00:00

abstract：:The replication control circuit of the FII plasmids R1, R100 and R6-5 involves a small untranslated RNA molecule of about 93 nucleotides. This RNA, designated CopA RNA in plasmid R1, acts in vivo as a post-transcriptional inhibitor of the expression of the RepA protein whose synthesis is required for each round of pla...

journal_title：Nucleic acids research

authors： Gerhart E,Wagner H,Nordström K

更新日期：1986-03-25 00:00:00

abstract：:The Chinese hamster cell line, DC-3F, is heterozygous at the DHFR locus, and each allele can be distinguished on the basis of a unique DNA restriction pattern, protein isoelectric profile and in the abundancy of the DHFR mRNAs it expresses. Although each allele produces four transcripts, 1000, 1650 and 2150 nucleotide...

journal_title：Nucleic acids research

authors： Scotto KW,Yang H,Davide JP,Melera PW

更新日期：1992-12-25 00:00:00

abstract：:Chlorella virus DNA ligase is the smallest eukaryotic ATP-dependent DNA ligase known; it suffices for yeast cell growth in lieu of the essential yeast DNA ligase Cdc9. The Chlorella virus ligase-adenylate intermediate has an intrinsic nick sensing function and its DNA footprint extends 8-9 nt on the 3'-hydroxyl (3'-OH...

journal_title：Nucleic acids research

authors： Odell M,Malinina L,Sriskanda V,Teplova M,Shuman S

更新日期：2003-09-01 00:00:00

abstract：:A mathematical model and a computer simulation were used to study PCR specificity. The model describes the occurrences of non-targeted PCR products formed through random primer-template interactions. The PCR simulation scans DNA sequence databases with primers pairs. According to the model prediction, PCR with complex...

journal_title：Nucleic acids research

authors： Rubin E,Levy AA

更新日期：1996-09-15 00:00:00

abstract：:Currently transgenic frog embryos are generated using restriction-enzyme-mediated integration (REMI) on decondensed sperm nuclei followed by nuclear transplantation into unfertilized eggs. We have developed a simplified version of this protocol that has the potential to increase the numbers of normally developing tran...

journal_title：Nucleic acids research

authors： Sparrow DB,Latinkic B,Mohun TJ

更新日期：2000-02-15 00:00:00