Abstract:
:We studied the interaction of ethidium bromide with rye and calf thymus chromatin. Both types of chromatin have the same dye accessibility, which is about 50% of that of DNA. From this result we conclude that the molecular structure of these two chromatins is similar. For rye, the extraction of H1 produces no change in the binding of ethidium bromide. The subsequent extraction of H2A and H2B produces a 14% increase in the binding, and the removal of H3 and H4, another 54% increase. At this stage, the number of binding sites is still less than that of DNA. This is presumably due to the presence of some tightly bound non-histones. Thus, the arginine-rich histones and the tightly bound non-histones are most responsible for limiting the binding of ethidium bromide to rye chromatin.
journal_name
Nucleic Acids Resjournal_title
Nucleic acids researchauthors
LaRue H,Pallotta Ddoi
10.1093/nar/3.9.2193subject
Has Abstractpub_date
1976-09-01 00:00:00pages
2193-206issue
9eissn
0305-1048issn
1362-4962journal_volume
3pub_type
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