Abstract:
:The Department of Energy (DOE) Joint Genome Institute (JGI) is a national user facility with massive-scale DNA sequencing and analysis capabilities dedicated to advancing genomics for bioenergy and environmental applications. Beyond generating tens of trillions of DNA bases annually, the Institute develops and maintains data management systems and specialized analytical capabilities to manage and interpret complex genomic data sets, and to enable an expanding community of users around the world to analyze these data in different contexts over the web. The JGI Genome Portal (http://genome.jgi.doe.gov) provides a unified access point to all JGI genomic databases and analytical tools. A user can find all DOE JGI sequencing projects and their status, search for and download assemblies and annotations of sequenced genomes, and interactively explore those genomes and compare them with other sequenced microbes, fungi, plants or metagenomes using specialized systems tailored to each particular class of organisms. We describe here the general organization of the Genome Portal and the most recent addition, MycoCosm (http://jgi.doe.gov/fungi), a new integrated fungal genomics resource.
journal_name
Nucleic Acids Resjournal_title
Nucleic acids researchauthors
Grigoriev IV,Nordberg H,Shabalov I,Aerts A,Cantor M,Goodstein D,Kuo A,Minovitsky S,Nikitin R,Ohm RA,Otillar R,Poliakov A,Ratnere I,Riley R,Smirnova T,Rokhsar D,Dubchak Idoi
10.1093/nar/gkr947subject
Has Abstractpub_date
2012-01-01 00:00:00pages
D26-32issue
Database issueeissn
0305-1048issn
1362-4962pii
gkr947journal_volume
40pub_type
杂志文章abstract::We have studied the structure of recombinant rat UBF (rrUBF), an RNA polymerase I transcription factor, by electron microscopy and image analysis of single particles contrasted with methylamine tungstate. Recombinant rat UBF appeared to be a flat, U-shaped protein with a central region of low density. In the dominant ...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/24.8.1472
更新日期:1996-04-15 00:00:00
abstract::The genome stability is maintained by coordinated action of DNA repairs and checkpoints, which delay progression through the cell cycle in response to DNA damage. Rad9 is conserved from yeast to human and functions in cell cycle checkpoint controls. Here, a regulatory mechanism for Rad9 function is reported. In this s...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkq1264
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journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkh991
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journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gky418
更新日期:2018-07-02 00:00:00
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journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/21.11.2737
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abstract::Recent studies employing genome-wide approaches have provided an unprecedented view of the scope of L1 activities on structural variations in the human genome, and further reinforced the role of L1s as one of the major driving forces behind human genome evolution. The rapid identification of novel L1 elements by these...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkq1076
更新日期:2011-02-01 00:00:00
abstract::Vaccinia virus DNA topoisomerase catalyzes resolution of synthetic Holliday junctions in vitro. The mechanism entails concerted transesterifications at two recognition sites, 5'-CCCTT/, that are opposed within a partially mobile four-way junction. Efficient resolution occurs on a junction with a 10 bp segment of branc...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/28.14.2658
更新日期:2000-07-15 00:00:00
abstract::ModBase (http://salilab.org/modbase) is a database of annotated comparative protein structure models. The models are calculated by ModPipe, an automated modeling pipeline that relies primarily on Modeller for fold assignment, sequence-structure alignment, model building and model assessment (http://salilab.org/modelle...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkt1144
更新日期:2014-01-01 00:00:00
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journal_title:Nucleic acids research
pub_type:
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journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/17.2.499
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abstract::The deoxyribose hexanucleoside pentaphosphate (m5dC-dG)3 has been studied by 500 MHz 1H NMR in D2O (0.1 M NaCl) and in D2O/deuterated methanol mixtures. Two conformations, in slow equilibrium on the NMR time scale, were detected in methanolic solution. Two-dimensional nuclear Overhauser effect (NOE) experiments were u...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/12.2.1243
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journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gks1055
更新日期:2013-01-01 00:00:00
abstract::50 to 55% of tobacco and barley nuclear DNA is accessible to micrococcal endonuclease digestion. The DNA fragments resulting from a mild endonuclease treatment are multiples of a basic unit of 194 +/- 6 base pairs in tobacco and 195 +/- 6 base pairs in barley. After extensive digestion, a DNA fragment of approximately...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/4.10.3617
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abstract::SV40 DNA form I is expressed efficiently after its injection into the nuclei of Xenopus laevis oocytes, resulting in the synthesis of RNA and protein products of both viral late and early transcription units. However it was observed that injection of SV40 genes cloned into pBR322 or related plasmids yielded vastly red...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/15.4.1579
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abstract::PPARγ2 is a critical lineage-determining transcription factor that is essential for adipogenic differentiation. Here we report characterization of the three-dimensional structure of the PPARγ2 locus after the onset of adipogenic differentiation and the mechanisms by which it forms. We identified a differentiation-depe...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkw129
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journal_title:Nucleic acids research
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doi:10.1093/nar/gkp1236
更新日期:2010-05-01 00:00:00
abstract::Non-homologous end-joining (NHEJ) of DNA double-strand breaks (DSBs) is mediated by two protein complexes comprising Ku80/Ku70/DNA-PKcs/Artemis and XRCC4/LigaseIV/XLF. Loss of Ku or XRCC4/LigaseIV function compromises the rejoining of radiation-induced DSBs and leads to defective V(D)J recombination. In this study, we...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkn094
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journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkg914
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abstract::A new restriction endonuclease Bse SI has been isolated from Bacillus stearothermophilus Jo10-553. Bse SI recognizes a degenerate hexanucleotide sequence 5'-G(G/T)GC(A/C)C-3' and cleaves DNA to produce 3[prime]-protruding tetranucleotide ends. ...
journal_title:Nucleic acids research
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abstract::Bacteria belonging to the class Mollicutes were among the first ones to be selected for complete genome sequencing because of the minimal size of their genomes and their pathogenicity for humans and a broad range of animals and plants. At this time six genome sequences have been publicly released (Mycoplasma genitaliu...
journal_title:Nucleic acids research
pub_type: 杂志文章
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journal_title:Nucleic acids research
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journal_title:Nucleic acids research
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journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/20.18.4919
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journal_title:Nucleic acids research
pub_type: 杂志文章
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journal_title:Nucleic acids research
pub_type: 杂志文章
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更新日期:2018-01-04 00:00:00
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journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/11.23.8485
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journal_title:Nucleic acids research
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journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/25.19.3795
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journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkm311
更新日期:2007-07-01 00:00:00
abstract::The paper describes an improved method for the synthesis of oligodeoxyribonucleotides using phosphoramidite chemistry. Our procedure relies on novel phosphoramidite intermediates, the deoxyribonucleoside-3'-morpholine-methoxyphosphins. These compounds are extremely stable and can be purified readily. Condensation reac...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/11.9.2575
更新日期:1983-05-11 00:00:00