Abstract：
:We describe improve enzymatic methods for sequencing method for sequencing DNA. They are based on partial digestion of duplex DNA with exonuclease III to produce DNA molecules with 3' ends shortened to varying lengths, followed by repair synthesis to extend and label the 3' ends. After asymmetrical cleavage of the DNA with a restriction enzyme, the labeled products are separated by gel electrophoresis and the sequence read from the autoradiogram. The entire procedures, beginning with unrestricted DNA and followed through gel electrophoresis, takes only one day for sequencing both strands of the DNA molecule. These methods are especially suitable for sequencing DNA cloned in plasmid vectors, and they greatly extend the usefulness of the dideoxynucleotide chain termination method of Sanger et al. (Proc. Natl. Acad. Sci. USA 74, 5463, 1977). Using these methods we have determined the sequence of a 410 base pair fragment which includes the yeast SUP3 tyrosine tRNA gene.
journal_name
Nucleic Acids Resjournal_title
Nucleic acids researchauthors
Guo LH,Wu Rdoi
10.1093/nar/10.6.2065subject
Has Abstractpub_date
1982-03-25 00:00:00pages
2065-84issue
6eissn
0305-1048issn
1362-4962journal_volume
10pub_type
杂志文章abstract：:Within a single infected individual, a virus population can have a high genomic variability. In the case of HIV, several mutations can be present even in a small genomic window of 20-30 nucleotides. For diagnostics purposes, it is often needed to resequence genomic subsets where crucial mutations are known to occur. I...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkt682
更新日期：2013-10-01 00:00:00
abstract：:The gene encoding the DNA methyltransferase M.CviRI from Chlorella virus XZ-6E was cloned and expressed in Escherichia coli. M.CviRI methylates adenine in TGCA sequences. DNA containing the M.CviRI gene was sequenced and a single open reading frame of 1137 bp was identified which could code for a polypeptide of 379 am...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/19.2.307
更新日期：1991-01-25 00:00:00
abstract：:Bacillus subtilis LrpC is a sequence-independent DNA-binding and DNA-bending protein, which binds both single-stranded (ss) and double-stranded (ds) DNA and facilitates the formation of higher order protein-DNA complexes in vitro. LrpC binds at different sites within the same DNA molecule promoting intramolecular liga...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkj418
更新日期：2006-01-10 00:00:00
abstract：:The centromere complex is a multicomponent structure essential for faithful chromosome transmission. Here we show that the S. cerevisiae centromere protein Cpf1 bends centromere DNA element I (CDEI) with the bend angle ranging from 66 degrees to 71 degrees. CDEI DNA sequences that carry point mutations which lead to r...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/21.20.4726
更新日期：1993-10-11 00:00:00
abstract：:The particular behaviour of eukaryotic RNA polymerases along different gene regions and amongst distinct gene functional groups is not totally understood. To cast light onto the alternative active or backtracking states of RNA polymerase II, we have quantitatively mapped active RNA polymerases at a high resolution fol...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gku1349
更新日期：2015-01-01 00:00:00
abstract：:Synthetic oligonucleotides were used to introduce mutations into the lacPUV5 promoter. Four mutations were obtained at positions -13, -14, and -15, with respect to the transcriptional start site. The effects of these mutations were measured in vivo and the results are discussed with respect to the consensus sequence a...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/12.9.4011
更新日期：1984-05-11 00:00:00
abstract：:RNA sequencing has become an important method to perform hypothesis-free characterization of global gene expression. One of the limitations of RNA sequencing is that most sequence reads represent highly expressed transcripts, whereas low level transcripts are challenging to detect. To combine the benefits of tradition...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gks816
更新日期：2013-01-07 00:00:00
abstract：:Toxin-antitoxin systems are widespread in bacteria and archaea. They perform diverse functional roles, including the generation of persistence, maintenance of genetic loci and resistance to bacteriophages through abortive infection. Toxin-antitoxin systems have been divided into three types, depending on the nature of...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gks231
更新日期：2012-07-01 00:00:00
abstract：:Saccharomyces cerevisiae Pif1 (ScPif1) is known as an ATP-dependent DNA helicase that plays critical roles in a number of important biological processes such as DNA replication, telomere maintenance and genome stability maintenance. Besides its DNA helicase activity, ScPif1 is also known as a single-stranded DNA (ssDN...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkz541
更新日期：2019-08-22 00:00:00
abstract：:The base pairing properties of oligonucleotide duplexes containing 8-aza-7-deaza-2'-deoxyisoguanosine, its 7-bromo or its 7-iodo derivative are described. The nucleosides were synthesized on a convergent route, protected and converted into phosphoramidites. Oligonucleotides were prepared on a solid-phase and were hybr...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkg926
更新日期：2003-12-15 00:00:00
abstract：:The DECIPHER database (https://decipher.sanger.ac.uk/) is an accessible online repository of genetic variation with associated phenotypes that facilitates the identification and interpretation of pathogenic genetic variation in patients with rare disorders. Contributing to DECIPHER is an international consortium of >2...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkt937
更新日期：2014-01-01 00:00:00
abstract：:Most small nucleolar RNAs (snoRNAs) guide rRNA nucleotide modifications, some participate in pre-rRNA cleavages, and a few have both functions. These activities involve direct base-pairing of the snoRNA with pre-rRNA using different domains. It is not known if the modification and processing functions occur independen...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkq043
更新日期：2010-06-01 00:00:00
abstract：:Helicobacter pylori is a Gram-negative bacterium that colonizes human stomach and causes gastric inflammation. The species is naturally competent and displays remarkable diversity. The presence of a large number of restriction-modification (R-M) systems in this bacterium creates a barrier against natural transformatio...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkt024
更新日期：2013-03-01 00:00:00
abstract：:Detailed models are presented for the triple-stranded polynucleotide helices of poly (U)-poly (A)-poly (U) (two forms), poly (U)-poly d (A) -poly (U), poly d(C)-poly d(I)-poly d(C), poly d(T)-polyd(A)-poly d(T) and poly (I)-poly (A)-poly (I). The models were genrated using a computerized, linked-atom procedure which p...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/3.10.2459
更新日期：1976-10-01 00:00:00
abstract：:Aminoglycosides antibiotics negate dissociation and recycling of the bacterial ribosome's subunits by binding to Helix 69 (H69) of 23S rRNA. The differential binding of various aminoglycosides to the chemically synthesized terminal domains of the Escherichia coli and human H69 has been characterized using spectroscopy...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkp1253
更新日期：2010-05-01 00:00:00
abstract：:Dlx2, Lymphoid Enhancer Factor (Lef-1) and Msx2 transcription factors are required for several developmental processes. To understand the control of gene expression by these factors, chromatin immunoprecipitation (ChIP) assays identified Msx2 as a downstream target of Dlx2 and Lef-1. Dlx2 activates the Msx2 promoter i...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkl689
更新日期：2006-01-01 00:00:00
abstract：:It has been shown that the monomethylated cap structure plays important roles in pre-mRNA splicing and nuclear export of RNA. As a candidate for the factor involved in these nuclear events we have previously purified an 80 kDa nuclear cap binding protein (NCBP) from a HeLa cell nuclear extract and isolated its full-le...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/23.18.3638
更新日期：1995-09-25 00:00:00
abstract：:No analogous nucleoside triphosphate was found which acts as well as ATP in binding to and supporting catalysis of leucyl-tRNA synthetase from Escherichia coli MRE 600. However, there are numerous nucleotides which are able to replace ATP, but with lower efficiency. The 6-amino group of the adenine ring and the 2'-hyd...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/3.8.2067
更新日期：1976-08-01 00:00:00
abstract：:Recent research hints at an underappreciated complexity in pre-miRNA processing and regulation. Global profiling of pre-miRNA and its potential to increase understanding of the pre-miRNA landscape is impeded by overlap with highly expressed classes of other non coding (nc) RNA. Here, we present a data set excluding th...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkr903
更新日期：2012-02-01 00:00:00
abstract：:Sox proteins belong to the HMG box superfamily of DNA-binding proteins and are found throughout the animal kingdom. They are involved in the regulation of such diverse developmental processes as germ layer formation, organ development and cell type specifi-cation. Hence, deletion or mutation of Sox proteins often resu...
journal_title：Nucleic acids research
pub_type： 杂志文章,评审
doi：10.1093/nar/27.6.1409
更新日期：1999-03-15 00:00:00
abstract：:Although a vast amount of life sciences data is generated in the form of images, most scientists still store images on extremely diverse and often incompatible storage media, without any type of metadata structure, and thus with no standard facility with which to conduct searches or analyses. Here we present a solutio...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/29.1.336
更新日期：2001-01-01 00:00:00
abstract：:We have used fluorescein-11-dUTP in a nick-translation format to produce fluoresceinated human nucleic acid probes. After in situ hybridization of fluoresceinated DNAs to human metaphase chromosomes, the detection sensitivity was found to be 50-100 kb. The feasibility and the increase in detection sensitivity of micro...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/19.12.3237
更新日期：1991-06-25 00:00:00
abstract：:Saccharomyces cerevisiae chl1 mutants have a significant increase in the rate of chromosome missegregation. CHL1 encodes a 99 kDa predicted protein with an ATP binding site consensus, a putative helix-turn-helix DNA binding motif, and homology to helicases. Using site-directed mutagenesis, I show that mutations that a...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/28.16.3056
更新日期：2000-08-15 00:00:00
abstract：:mRNA deadenylation is under the control of cis-acting regulatory elements, which include AU-rich elements (AREs) and microRNA (miRNA) targeting sites, within the 3' untranslated region (3' UTRs) of eukaryotic mRNAs. Deadenylases promote miRNA-induced mRNA decay through their interaction with miRNA-induced silencing co...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkv959
更新日期：2015-12-15 00:00:00
abstract：:We describe a rapid fluorescence indicator displacement assay (R-FID) to evaluate the affinity and the selectivity of compounds binding to different DNA structures. We validated the assay using a library of 30 well-known nucleic acid binders containing a variety chemical scaffolds. We used a combination of principal c...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gky019
更新日期：2018-04-20 00:00:00
abstract：:The design and synthesis of metal complexes that can specifically target DNA secondary structure has attracted considerable attention. Chiral metallosupramolecular complexes (e.g. helicates) in particular display unique DNA-binding behavior, however until recently few examples which are both water-compatible and enant...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkx244
更新日期：2017-05-19 00:00:00
abstract：:The RESID Database is a comprehensive collection of annotations and structures for protein post-translational modifications including N-terminal, C-terminal and peptide chain cross-link modifications. The RESID Database includes systematic and frequently observed alternate names, Chemical Abstracts Service registry nu...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/29.1.199
更新日期：2001-01-01 00:00:00
abstract：:The alternation/deficiency in activation-3 (ADA3) is an essential component of the human p300/CBP-associated factor (PCAF) and yeast Spt-Ada-Gcn5-acetyltransferase (SAGA) histone acetyltransferase complexes. These complexes facilitate transactivation of target genes by association with transcription factors and modifi...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkq269
更新日期：2010-09-01 00:00:00
abstract：:The transcripts of a 6Kbp region of the mitochondrial genome of S. cerevisiae, localized in the 21S rRNA-OXI1 span and including 12 tRNA genes (from tRNA(thr) to tRNA(ala)) and several G+C clusters, have been studied by analysis of in vitro capped primary transcripts and by fine mapping of the 5' ends of transcripts. ...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/15.16.6387
更新日期：1987-08-25 00:00:00
abstract：:To organize data resulting from the phenotypic characterization of a library of 30,000 T-DNA enhancer trap (ET) insertion lines of rice (Oryza sativa L cv. Nipponbare), we developed the Oryza Tag Line (OTL) database (http://urgi.versailles.inra.fr/OryzaTagLine/). OTL structure facilitates forward genetic search for sp...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkm762
更新日期：2008-01-01 00:00:00
