Abstract:
:We have isolated the gene coding for the U2 analogue in trypanosomes. The 148 nucleotide long U2 RNA is capped and transcribed from a single copy gene. The 5' half of the molecule is highly homologous to mammalian U2 RNA, while the 3' half does not show any significant sequence homology with the mammalian counterpart. Nevertheless, the trypanosome U2 RNA can be folded into a secondary structure resembling the one proposed for U2 RNA. The presence of a U2 analogue and most likely other U RNAs in trypanosomes suggests that splicing is involved at some point in the maturation of mRNA. Possible interactions of the U2 RNA with the spliced leader RNA are considered.
journal_name
Nucleic Acids Resjournal_title
Nucleic acids researchauthors
Tschudi C,Richards FF,Ullu Edoi
10.1093/nar/14.22.8893subject
Has Abstractpub_date
1986-11-25 00:00:00pages
8893-903issue
22eissn
0305-1048issn
1362-4962journal_volume
14pub_type
杂志文章abstract::With discovery of diverse roles for RNA, its centrality in cellular functions has become increasingly apparent. A number of algorithms have been developed to predict RNA secondary structure. Their performance has been benchmarked by comparing structure predictions to reference secondary structures. Generally, algorith...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkr1081
更新日期:2012-02-01 00:00:00
abstract::Flap endonuclease-1 (FEN1) is a member of the Rad2 structure-specific nuclease family. FEN1 possesses FEN, 5'-exonuclease and gap-endonuclease activities. The multiple nuclease activities of FEN1 allow it to participate in numerous DNA metabolic pathways, including Okazaki fragment maturation, stalled replication fork...
journal_title:Nucleic acids research
pub_type: 杂志文章,评审
doi:10.1093/nar/gkq884
更新日期:2011-02-01 00:00:00
abstract::We have implemented a genome annotation system for prokaryotes called AGMIAL. Our approach embodies a number of key principles. First, expert manual annotators are seen as a critical component of the overall system; user interfaces were cyclically refined to satisfy their needs. Second, the overall process should be o...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkl471
更新日期:2006-07-19 00:00:00
abstract::Transcriptional co-activator LEDGF/p75 is the major cellular interactor of HIV-1 integrase (IN), critical to efficient viral replication. In this work, a series of INs from the Betaretrovirus, Gammaretrovirus, Deltaretrovirus, Spumavirus and Lentivirus retroviral genera were tested for interaction with the host factor...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkl885
更新日期:2007-01-01 00:00:00
abstract::The sequence of 436 nucleotides around the region coding for 5.8S RNA in the Sciara coprophila rDNA transcription unit (1) has been determined. Regions coding for 5.8S and 2S RNAs have been identified; they are 80 - 90% homologous to the corresponding Drosophila sequences and are separated by a 22 nucleotide long spac...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/8.16.3565
更新日期:1980-08-25 00:00:00
abstract::Foldback DNA, prepared from mouse and Scilla sibirica main band DNA, and from rye (Secale cereale) total DNA, was characterised by denaturation, renaturation, and electron microscopy. 3H-cRNA of this DNA was hybridised in situ to nuclei and chromosomes of the respective species. There is no universal labelling pattern...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/5.10.3589
更新日期:1978-10-01 00:00:00
abstract::The somatic V(D)J recombination for the assembly of the Ig and TCR genes is mediated by the recombination signal sequences (Rss) and the V(D)J recombinase. A cDNA clone was isolated from a lambda gt11 expression library made from mouse thymocyte poly(A)+ RNA, using the Rss as a ligand. The deduced amino acid sequence ...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/21.22.5067
更新日期:1993-11-11 00:00:00
abstract::The detailed principles of the hierarchical folding of eukaryotic chromosomes have been revealed during the last two decades. Along with structures composing three-dimensional (3D) genome organization (chromatin compartments, topologically associating domains, chromatin loops, etc.), the molecular mechanisms that are ...
journal_title:Nucleic acids research
pub_type: 杂志文章,评审
doi:10.1093/nar/gkaa261
更新日期:2020-05-21 00:00:00
abstract::The Phospho.ELM resource (http://phospho.elm.eu.org) is a relational database designed to store in vivo and in vitro phosphorylation data extracted from the scientific literature and phosphoproteomic analyses. The resource has been actively developed for more than 7 years and currently comprises 42,574 serine, threoni...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkq1104
更新日期:2011-01-01 00:00:00
abstract::R.E.D. Server is a unique, open web service, designed to derive non-polarizable RESP and ESP charges and to build force field libraries for new molecules/molecular fragments. It provides to computational biologists the means to derive rigorously molecular electrostatic potential-based charges embedded in force field l...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkr288
更新日期:2011-07-01 00:00:00
abstract::Eukaryotic cells use numerous pathways to regulate RNA production, localization and stability. Several of these pathways are controlled by ribonucleases. The essential ribonuclease, Dis3, plays important roles in distinct RNA metabolic pathways. Despite much progress in understanding general characteristics of the Dis...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkq295
更新日期:2010-09-01 00:00:00
abstract::We identify a new subgroup of Type I Restriction-Modification enzymes that modify cytosine in one DNA strand and adenine in the opposite strand for host protection. Recognition specificity has been determined for ten systems using SMRT sequencing and each recognizes a novel DNA sequence motif. Previously characterized...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkw743
更新日期:2016-11-02 00:00:00
abstract::Evidence has emerged that repair of clustered DNA lesions may be compromised, possibly leading to the formation of double-strand breaks (DSB) and, thus, to deleterious events. The first repair event occurring at a multiply damaged site (MDS) is of major importance and will largely contribute to the hazardousness of MD...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkm190
更新日期:2007-01-01 00:00:00
abstract::The conformations of supercoiled (sc) DNA and linear DNA bound to polylysine (PL)-coated mica were investigated by scanning force microscopy (SFM) in solution. From the polymer statistical analysis of linear DNA, we could distinguish between re-arrangements or trapping of the DNA on the surface. Conditions of re-arran...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gng137
更新日期:2003-11-15 00:00:00
abstract::MicroRNAs (miRNA) are short single-stranded RNA molecules derived from hairpin-forming precursors that play a crucial role as post-transcriptional regulators in eukaryotes and viruses. In the past years, many microRNA target genes (MTGs) have been identified experimentally. However, because of the high costs of experi...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkw1085
更新日期:2017-01-25 00:00:00
abstract::The enterovirus 71 (EV71) 3Dpol is an RNA-dependent RNA polymerase (RdRP) that plays the central role in the viral genome replication, and is an important target in antiviral studies. Here, we report a crystal structure of EV71 3Dpol elongation complex (EC) at 1.8 Å resolution. The structure reveals that the 5'-end gu...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkz1170
更新日期:2020-02-20 00:00:00
abstract::The chromatographic profiles of isoaccepting tRNAs were analyzed at five time points during the 96 hr, dimethylsulfoxide induced, erythroid-like differentiation of Friend leukemia cells. Sixty-four isoaccepting species of tRNA for 16 amino acids were resolved by RPC-5 chromatography. The relative amounts of tRNAphe, t...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/8.15.3467
更新日期:1980-08-11 00:00:00
abstract::Deciphering gene regulatory network architecture amounts to the identification of the regulators, conditions in which they act, genes they regulate, cis-acting motifs they bind, expression profiles they dictate and more complex relationships between alternative regulatory partnerships and alternative regulatory motifs...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gki166
更新日期:2005-01-31 00:00:00
abstract::The nucleotide sequence of the promoter region and the first five genes of the atp (or unc) operon of Escherichia coli has been determined. The first proposed gene in the operon contains four AUA codons and may be poorly expressed; it encodes a basic but yet hydrophobic protein which could function as a pilot protein ...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/9.16.3919
更新日期:1981-08-25 00:00:00
abstract::Transactivation by the ETS family of transcription factors, whose members share structurally conserved DNA-binding domains, is variably sensitive to methylation of their target genes. The mechanism by which DNA methylation controls ETS proteins remains poorly understood. Uncertainly also pervades the effects of hemi-m...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkw528
更新日期:2016-10-14 00:00:00
abstract::To study the role of the poly(A) tail length during the replication of poly(A)-containing plus-strand RNA virus, we have developed a simple reverse transcription polymerase chain reaction (RT-PCR)-based method that substantially improves the previously reported PAT [poly(A) test] assay. In contrast to the PAT assay, t...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/29.12.e57
更新日期:2001-06-15 00:00:00
abstract::We have isolated and sequenced the complete murine I-E alpha immune response gene of the H-2db haplotype. The I-E alpha d gene consists of 5300 basepairs and is organized into five or possibly six exons that correspond to different domains of the alpha chain. The amino acid sequence deduced from the I-E alpha gene sho...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/11.15.5055
更新日期:1983-08-11 00:00:00
abstract::The recent outbreak of COVID-19 has generated an enormous amount of Big Data. To date, the COVID-19 Open Research Dataset (CORD-19), lists ∼130,000 articles from the WHO COVID-19 database, PubMed Central, medRxiv, and bioRxiv, as collected by Semantic Scholar. According to LitCovid (11 August 2020), ∼40,300 COVID19-re...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkaa969
更新日期:2021-01-08 00:00:00
abstract::The NusA protein is a universally conserved bacterial transcription elongation factor that binds RNA polymerase (RNAP). When functioning independently, NusA enhances intrinsic termination. Paradoxically, NusA stimulates the function of the N and Q antiterminator proteins of bacteriophage λ. The mechanistic basis for N...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkw198
更新日期:2016-06-20 00:00:00
abstract::Toxicogenomics focuses on assessing the safety of compounds using gene expression profiles. Gene expression signatures from large toxicogenomics databases are expected to perform better than small databases in identifying biomarkers for the prediction and evaluation of drug safety based on a compound's toxicological m...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gku955
更新日期:2015-01-01 00:00:00
abstract::In testis mRNA stability and translation initiation are extensively under the control of poly(A)-binding proteins (PABP). Here we have cloned a new human testis-specific PABP (PABP3) of 631 amino acids (70.1 kDa) with 92.5% identical residues to the ubiquitous PABP1. A northern blot of multiple human tissues hybridise...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/29.9.1872
更新日期:2001-05-01 00:00:00
abstract::Gene losses provide an insightful route for studying the morphological and physiological adaptations of species, but their discovery is challenging. Existing genome annotation tools focus on annotating intact genes and do not attempt to distinguish nonfunctional genes from genes missing annotation due to sequencing an...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkaa550
更新日期:2020-09-18 00:00:00
abstract::The targeted adduction of aflatoxin B1- exo -8,9-epoxide (AFB1- exo -8,9-epoxide) to a specific guanine within an oligodeoxyribonucleotide containing multiple guanines was achieved using a DNA triplex to control sequence selectivity. The oligodeoxyribonucleotide d(AGAGAAGATTTTCTTCTCTTTTTTTTCTCTT), designated '3G', spo...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/26.4.1070
更新日期:1998-02-15 00:00:00
abstract::7SK snRNA, an abundant RNA discovered in human nucleus, regulates transcription by RNA polymerase II (RNAPII). It sequesters and inhibits the transcription elongation factor P-TEFb which, by phosphorylation of RNAPII, switches transcription from initiation to processive elongation and relieves pauses of transcription....
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkq660
更新日期:2010-11-01 00:00:00
abstract::Human immunodeficiency virus-1 (HIV-1) leader RNA, which contains double-stranded regions due to inverted repeats, was shown to activate the dsRNA-dependent enzymes associated with the interferon system. HIV-1 leader RNA produced in vitro using SP6 RNA polymerase was characterized using probes for antisense and sense-...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/17.3.969
更新日期:1989-02-11 00:00:00