Elements within the beta-lactoglobulin gene inhibit expression of human serum albumin cDNA and minigenes in transfected cells but rescue their expression in the mammary gland of transgenic mice.


:Two new beta-lactoglobulin (BLG)/human serum albumin (HSA) hybrid gene vectors were constructed and tested for expression in COS-7 cells and in transgenic mice. The HSA sequences were inserted between the second and sixth BLG exons. Transient transfection experiments with these vectors as well as a series of additional vectors with either the BLG 5'- or 3'- intragenic sequences revealed that sequences within BLG exon 1/intron 1/exon 2 abrogated BLG- directed HSA expression in vitro, regardless of the presence of HSA introns or the origin of the 3' polyadenylation signal. In contrast, the same BLG expression cassette enabled the efficient expression of HSA cDNA or minigene in the mammary gland of transgenic mice with subsequent secretion of the corresponding protein into the milk of 56 and 82%, respectively of the mouse strains at levels up to 0.3 mg/ml. Previous attempts to express HSA cDNA inserted into exon 1 of the BLG gene had failed [Shani,M., Barash,I., Nathan,M., Ricca,G., Searfoss,G.H., Dekel,I., Faerman,A., Givol,D. and Hurwitz,D.R. (1992) Transgenic Res. 1, 195- 208]. The new BLG expression cassette conferred more stringent tissue specific expression than previously described BLG/HSA constructs [Barash,I, Faerman,A., Ratovitsky,T, Puzis,R., Nathan,M., Hurwitz,D.R. and Shani, M. (1994) Transgenic Res. 3, 141-151]. However, it was not able to insulate the transgenes from the surrounding host DNA sequences and did not result in copy number dependent expression in transgenics. Together, the in vitro and in vivo results suggest both positive and negative regulatory elements within the BLG intragenic sequences evaluated. The new BLG construct represents an extremely valuable vector for the efficient expression of cDNAs in the mammary gland of transgenic animals.


Nucleic Acids Res


Nucleic acids research


Barash I,Nathan M,Kari R,Ilan N,Shani M,Hurwitz DR




Has Abstract


1996-02-15 00:00:00














  • Cryptic transcripts from a ubiquitous plasmid origin of replication confound tests for cis-regulatory function.

    abstract::A vast amount of research on the regulation of gene expression has relied on plasmid reporter assays. In this study, we show that plasmids widely used for this purpose constitutively produce substantial amounts of RNA from a TATA-containing cryptic promoter within the origin of replication. Readthrough of these RNAs i...

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    pub_type: 杂志文章


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  • WormNet v3: a network-assisted hypothesis-generating server for Caenorhabditis elegans.

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    journal_title:Nucleic acids research

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    authors: Cho A,Shin J,Hwang S,Kim C,Shim H,Kim H,Kim H,Lee I

    更新日期:2014-07-01 00:00:00

  • Two orthogonal cleavages separate subunit RNAs in mouse ribosome biogenesis.

    abstract::Ribosome biogenesis is a dynamic multistep process, many features of which are still incompletely documented. Here, we show that changes in this pathway can be captured and annotated by means of a graphic set of pre-rRNA ratios, a technique we call Ratio Analysis of Multiple Precursors (RAMP). We find that knocking do...

    journal_title:Nucleic acids research

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    authors: Wang M,Anikin L,Pestov DG

    更新日期:2014-01-01 00:00:00

  • Ultraviolet resonance Raman study of DNA and of its interaction with actinomycin D.

    abstract::The DNA-Actinomycin D interaction has been studied by resonance Raman effect using DNA as chromophore. First, the resonance Raman spectra of DNA obtained with a U.V. excitation at wavelengths of 300 nm and 280 nm are presented. The main Raman hands are assigned to the convenient nucleic bases by comparison with the sp...

    journal_title:Nucleic acids research

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    authors: Chinsky L,Turpin PY

    更新日期:1978-08-01 00:00:00

  • Microamplification of specific chromosome sequences; an improved method for genome analysis.

    abstract::An improved method was developed for microdissection and cloning of metaphase as well as pachytene chromosomes. The protocol incorporates efficient ligation of chromosomal DNA with linker adaptors, abolishment of microcloning steps and the reduction of micromanipulation. The threshold for amplifying genomic DNA templa...

    journal_title:Nucleic acids research

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    authors: Ponelies N,Stein N,Weber G

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  • The folding of the hepatitis C virus internal ribosome entry site depends on the 3'-end of the viral genome.

    abstract::Hepatitis C virus (HCV) translation initiation is directed by an internal ribosome entry site (IRES) and regulated by distant regions at the 3'-end of the viral genome. Through a combination of improved RNA chemical probing methods, SHAPE structural analysis and screening of RNA accessibility using antisense oligonucl...

    journal_title:Nucleic acids research

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    authors: Romero-López C,Barroso-Deljesus A,García-Sacristán A,Briones C,Berzal-Herranz A

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  • Nuclear transcriptional activity of the tobacco plastid psbA promoter.

    abstract::The plastid psbA promoter of tobacco was used with the aim to construct plastid specific marker genes. Upon transfer to the tobacco nuclear genome the plastid promoter fragment appeared to specify a messenger RNA. Placed 5' to the bar or nptII coding sequences the level of expression is sufficient to obtain a selectab...

    journal_title:Nucleic acids research

    pub_type: 杂志文章


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  • BLAST2SRS, a web server for flexible retrieval of related protein sequences in the SWISS-PROT and SPTrEMBL databases.

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    pub_type: 杂志文章


    authors: Bimpikis K,Budd A,Linding R,Gibson TJ

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  • chipD: a web tool to design oligonucleotide probes for high-density tiling arrays.

    abstract::chipD is a web server that facilitates design of DNA oligonucleotide probes for high-density tiling arrays, which can be used in a number of genomic applications such as ChIP-chip or gene-expression profiling. The server implements a probe selection algorithm that takes as an input, in addition to the target sequences...

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    authors: Dufour YS,Wesenberg GE,Tritt AJ,Glasner JD,Perna NT,Mitchell JC,Donohue TJ

    更新日期:2010-07-01 00:00:00

  • The human apolipoprotein A-II gene: complete nucleic acid sequence and genomic organization.

    abstract::The gene for human apolipoprotein (apo) A-II has been isolated from a human genomic DNA library. The cloned fragment was approximately 14 kilobase-pair (kb) long, and extended about 9.0 kb upstream as well as 3.5 kb downstream from the apoA-II gene, which was contained within a 3.1 kb HindIII fragment of human DNA. Th...

    journal_title:Nucleic acids research

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    authors: Lackner KJ,Law SW,Brewer HB Jr

    更新日期:1985-06-25 00:00:00

  • Stimuli of differentiation regulate RNA elongation in the transcription units for the major stage-specific antigens of Trypanosoma brucei.

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    journal_title:Nucleic acids research

    pub_type: 杂志文章


    authors: Vanhamme L,Berberof M,Le Ray D,Pays E

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  • FBXL5-mediated degradation of single-stranded DNA-binding protein hSSB1 controls DNA damage response.

    abstract::Human single-strand (ss) DNA binding proteins 1 (hSSB1) has been shown to participate in DNA damage response and maintenance of genome stability by regulating the initiation of ATM-dependent signaling. ATM phosphorylates hSSB1 and prevents hSSB1 from ubiquitin-proteasome-mediated degradation. However, the E3 ligase th...

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    authors: Chen ZW,Liu B,Tang NW,Xu YH,Ye XY,Li ZM,Niu XM,Shen SP,Lu S,Xu L

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    authors: Fennoy SL,Bailey-Serres J

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  • Protonatable hairpins are conserved in the 5'-untranslated region of tymovirus RNAs.

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    journal_title:Nucleic acids research

    pub_type: 杂志文章


    authors: Hellendoorn K,Michiels PJ,Buitenhuis R,Pleij CW

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  • Alternative 5' exons either provide or deny an initiator methionine codon to the same alpha-tubulin coding region.

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    authors: Bastian FB,Roux J,Niknejad A,Comte A,Fonseca Costa SS,de Farias TM,Moretti S,Parmentier G,de Laval VR,Rosikiewicz M,Wollbrett J,Echchiki A,Escoriza A,Gharib WH,Gonzales-Porta M,Jarosz Y,Laurenczy B,Moret P,Person E,

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    journal_title:Nucleic acids research

    pub_type: 杂志文章


    authors: Argos P

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  • Conditional genome engineering reveals canonical and divergent roles for the Hus1 component of the 9-1-1 complex in the maintenance of the plastic genome of Leishmania.

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  • Studies on the mechanism of DNA cleavage by ethidium.

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    authors: Deniss IS,Morgan AR

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    authors: Brzeski J,Podstolski W,Olczak K,Jerzmanowski A

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  • Flexibility and stabilization of HgII-mediated C:T and T:T base pairs in DNA duplex.

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    journal_title:Nucleic acids research

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    authors: Liu H,Cai C,Haruehanroengra P,Yao Q,Chen Y,Yang C,Luo Q,Wu B,Li J,Ma J,Sheng J,Gan J

    更新日期:2017-03-17 00:00:00

  • RosettaDesign server for protein design.

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    journal_title:Nucleic acids research

    pub_type: 杂志文章


    authors: Liu Y,Kuhlman B

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  • The Mouse Genome Database: integration of and access to knowledge about the laboratory mouse.

    abstract::The Mouse Genome Database (MGD) (http://www.informatics.jax.org) is the community model organism database resource for the laboratory mouse, a premier animal model for the study of genetic and genomic systems relevant to human biology and disease. MGD maintains a comprehensive catalog of genes, functional RNAs and oth...

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    authors: Blake JA,Bult CJ,Eppig JT,Kadin JA,Richardson JE,Mouse Genome Database Group.

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    journal_title:Nucleic acids research

    pub_type: 杂志文章


    authors: Turner PC,Bagenal EB,Vlad MT,Woodland HR

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  • Actin and myosin contribute to mammalian mitochondrial DNA maintenance.

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    journal_title:Nucleic acids research

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    authors: Reyes A,He J,Mao CC,Bailey LJ,Di Re M,Sembongi H,Kazak L,Dzionek K,Holmes JB,Cluett TJ,Harbour ME,Fearnley IM,Crouch RJ,Conti MA,Adelstein RS,Walker JE,Holt IJ

    更新日期:2011-07-01 00:00:00

  • RNA surveillance by uridylation-dependent RNA decay in Schizosaccharomyces pombe.

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    journal_title:Nucleic acids research

    pub_type: 杂志文章


    authors: Chung CZ,Jaramillo JE,Ellis MJ,Bour DYN,Seidl LE,Jo DHS,Turk MA,Mann MR,Bi Y,Haniford DB,Duennwald ML,Heinemann IU

    更新日期:2019-04-08 00:00:00

  • On the specificity and effects on transcription of P-element insertions at the yellow locus of Drosophila melanogaster.

    abstract::Fourteen P-element insertion mutants at the yellow (y) gene of D. melanogaster have been analysed by restriction mapping and DNA sequencing. The distribution of the insertion sites is nonrandom, with a preferred target site in the 5'-transcribed but non-translated leader of the gene. Transcription analysis indicates t...

    journal_title:Nucleic acids research

    pub_type: 杂志文章


    authors: Howes G,O'Connor M,Chia W

    更新日期:1988-04-11 00:00:00

  • A pentatricopeptide repeat protein acts as a site-specificity factor at multiple RNA editing sites with unrelated cis-acting elements in plastids.

    abstract::In plant organelles, RNA editing alters specific cytidine residues to uridine in transcripts. All of the site-specificity factors of RNA editing identified so far are pentatricopeptide repeat (PPR) proteins. A defect in a specific PPR protein often impairs RNA editing at multiple sites, at which the cis-acting element...

    journal_title:Nucleic acids research

    pub_type: 杂志文章


    authors: Okuda K,Shikanai T

    更新日期:2012-06-01 00:00:00

  • Motif3D: Relating protein sequence motifs to 3D structure.

    abstract::Motif3D is a web-based protein structure viewer designed to allow sequence motifs, and in particular those contained in the fingerprints of the PRINTS database, to be visualised on three-dimensional (3D) structures. Additional functionality is provided for the rhodopsin-like G protein-coupled receptors, enabling finge...

    journal_title:Nucleic acids research

    pub_type: 杂志文章


    authors: Gaulton A,Attwood TK

    更新日期:2003-07-01 00:00:00