Abstract:
:Replication of the promiscuous plasmid pMV158 requires expression of the initiator repB gene, which is controlled by the repressor CopG. Genes repB and copG are co-transcribed from promoter P(cr). We have studied the interactions between RNA polymerase, CopG and the promoter to elucidate the mechanism of repression by CopG. Complexes formed at 0 degrees C and at 37 degrees C between RNA polymerase and P(cr) differed from each other in stability and in the extent of the DNA contacted. The 37 degrees C complex was very stable (half-life of about 3 h), and shared features with typical open complexes generated at a variety of promoters. CopG protein repressed transcription from P(cr) at two different stages in the process leading to the initiation complex. First, CopG hindered binding of RNA polymerase to the promoter. Second, CopG was able to displace RNA polymerase once the enzyme has formed a stable complex with P(cr). A model for the CopG-mediated disassembly of the stable RNA polymerase-P(cr) promoter complex is presented.
journal_name
Nucleic Acids Resjournal_title
Nucleic acids researchauthors
Hernández-Arriaga AM,Rubio-Lepe TS,Espinosa M,del Solar Gdoi
10.1093/nar/gkp503subject
Has Abstractpub_date
2009-08-01 00:00:00pages
4799-811issue
14eissn
0305-1048issn
1362-4962pii
gkp503journal_volume
37pub_type
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journal_title:Nucleic acids research
pub_type: 杂志文章
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更新日期:2012-05-01 00:00:00
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journal_title:Nucleic acids research
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journal_title:Nucleic acids research
pub_type: 杂志文章
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更新日期:2003-07-01 00:00:00
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更新日期:1979-10-25 00:00:00
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更新日期:2014-11-10 00:00:00
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journal_title:Nucleic acids research
pub_type: 杂志文章
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更新日期:2005-05-24 00:00:00
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journal_title:Nucleic acids research
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更新日期:2005-10-21 00:00:00
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更新日期:1996-12-01 00:00:00
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journal_title:Nucleic acids research
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pub_type: 杂志文章
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更新日期:2000-11-01 00:00:00