Mutation detection using immobilized mismatch binding protein (MutS).

abstract：:Generation of double-stranded cDNA during reverse transcription of a variety of mRNA molecules is well known to involve the formation of covalently linked antisense and sense strands in a hairpin configuration. In the present study we have examined the sequence of molecular events which occurs during cDNA synthesis fr...

journal_title：Nucleic acids research

authors： Volloch VZ,Schweitzer B,Rits S

更新日期：1994-12-11 00:00:00

abstract：:By in vivo DMS footprint and reporter gene analyses we identified two transcription factor binding sites in the human c-sis/PDGF B gene promoter. The low basal activity of the PDGF B promoter in HeLa and undifferentiated K562 cells, which express low PDGF B mRNA levels, and in PC3 cells, which express a high PDGF B mR...

journal_title：Nucleic acids research

authors： Dirks RP,Jansen HJ,van Gerven B,Onnekink C,Bloemers HP

更新日期：1995-04-11 00:00:00

abstract：:OligoArrayDb is a comprehensive database containing pangenomic oligonucleotide microarray probe sets designed for most of the sequenced genomes that are not covered by commercial catalog arrays. The availability of probe sequences, associated with custom microarray fabrication services offered by many companies and co...

journal_title：Nucleic acids research

authors： Rouillard JM,Gulari E

更新日期：2009-01-01 00:00:00

abstract：:Genotoxic stress induces alternative splicing of the oncogene MDM2 generating MDM2-ALT1, an isoform attributed with tumorigenic properties. However, the mechanisms underlying this event remain unclear. Here we explore MDM2 splicing regulation by utilizing a novel minigene that mimics endogenous MDM2 splicing in respon...

journal_title：Nucleic acids research

authors： Comiskey DF Jr,Jacob AG,Singh RK,Tapia-Santos AS,Chandler DS

更新日期：2015-04-30 00:00:00

abstract：:The process of strand exchange is considered to be the hallmark of DNA recombination. Proteins known to carry out such exchange are believed to operate via one or the other of two mechanisms. RecA-like proteins promote the formation of a three-stranded or triplex synaptic intermediate in which strand exchange occurs, ...

journal_title：Nucleic acids research

authors： Jean D,Gendron D,Delbecchi L,Bourgaux P

更新日期：1997-10-15 00:00:00

abstract：:Saccharomyces cerevisiae Pif1 (ScPif1) is known as an ATP-dependent DNA helicase that plays critical roles in a number of important biological processes such as DNA replication, telomere maintenance and genome stability maintenance. Besides its DNA helicase activity, ScPif1 is also known as a single-stranded DNA (ssDN...

journal_title：Nucleic acids research

authors： Lu C,Le S,Chen J,Byrd AK,Rhodes D,Raney KD,Yan J

更新日期：2019-08-22 00:00:00

abstract：:SSCP and heteroduplex analysis (HA) continue to be the most popular methods of mutation detection due to their simplicity, high sensitivity and low cost. The advantages of these methods are most clearly visible when large genes, such as BRCA1 and BRCA2, are scanned for scattered unknown mutations and/or when a large n...

journal_title：Nucleic acids research

authors： Kozlowski P,Krzyzosiak WJ

更新日期：2001-07-15 00:00:00

abstract：:We have determined the complete nucleotide sequence of the regions of mouse ribosomal RNA transcription unit which separate mature rRNA genes. These internal transcribed spacers (ITS) are excised from rRNA precursor during ribosome biosynthesis. ITS 1, between 18S and 5.8S rRNA genes, is 999 nucleotides long. ITS 2, b...

journal_title：Nucleic acids research

authors： Michot B,Bachellerie JP,Raynal F

更新日期：1983-05-25 00:00:00

abstract：:Riboswitches are important gene regulatory elements frequently encountered in bacterial mRNAs. The recently discovered nadA riboswitch contains two similar, tandemly arrayed aptamer domains, with the first domain possessing high affinity for nicotinamide adenine dinucleotide (NAD+). The second domain which comprises t...

journal_title：Nucleic acids research

authors： Chen H,Egger M,Xu X,Flemmich L,Krasheninina O,Sun A,Micura R,Ren A

更新日期：2020-12-02 00:00:00

abstract：:Sets of genes expressed in the same tissue are believed to be under the regulation of a similar set of transcription factors, and can thus be assumed to contain similar structural patterns in their regulatory regions. Here we present a study of the structural patterns in promoters of genes expressed specifically in 26...

journal_title：Nucleic acids research

authors： Vandenbon A,Nakai K

更新日期：2010-01-01 00:00:00

abstract：:R spider is a web-based tool for the analysis of a gene list using the systematic knowledge of core pathways and reactions in human biology accumulated in the Reactome and KEGG databases. R spider implements a network-based statistical framework, which provides a global understanding of gene relations in the supplied ...

journal_title：Nucleic acids research

authors： Antonov AV,Schmidt EE,Dietmann S,Krestyaninova M,Hermjakob H

更新日期：2010-07-01 00:00:00

abstract：:Recruitment of RAD18 to stalled replication forks facilitates monoubiquitination of PCNA during S-phase, promoting translesion synthesis at sites of UV irradiation-induced DNA damage. In this study, we show that RAD18 is also recruited to ionizing radiation (IR)-induced sites of DNA double-strand breaks (DSBs) forming...

journal_title：Nucleic acids research

authors： Watanabe K,Iwabuchi K,Sun J,Tsuji Y,Tani T,Tokunaga K,Date T,Hashimoto M,Yamaizumi M,Tateishi S

更新日期：2009-04-01 00:00:00

abstract：:The U1 snRNP-specific protein C contains an N-terminal zinc finger-like CH motif which is required for the binding of the U1C protein to the U1 snRNP particle. Recently a similar motif was reported to be essential for in vivo homodimerization of the yeast splicing factor PRP9. In the present study we demonstrate that ...

journal_title：Nucleic acids research

authors： Gunnewiek JM,van Aarssen Y,Wassenaar R,Legrain P,van Venrooij WJ,Nelissen RL

更新日期：1995-12-11 00:00:00

abstract：:We report the complete DNA sequence of the short repeat region in the genome of herpes simplex virus type 1, as 6633 base pairs of composition 79.5% G+C. This contains immediate early gene 3, encoding the IE175 protein, an important transcriptional activator of later virus genes. The IE175 coding region was identified...

journal_title：Nucleic acids research

authors： McGeoch DJ,Dolan A,Donald S,Brauer DH

更新日期：1986-02-25 00:00:00

abstract：:ATRX and MeCP2 belong to an expanding group of chromatin-associated proteins implicated in human neurodevelopmental disorders, although their gene-regulatory activities are not fully resolved. Loss of ATRX prevents full repression of an imprinted gene network in the postnatal brain and in this study we address the mec...

journal_title：Nucleic acids research

authors： Kernohan KD,Vernimmen D,Gloor GB,Bérubé NG

更新日期：2014-07-01 00:00:00

abstract：:Polyadenylation is a co-transcriptional process that modifies mRNA 3'-ends in eukaryotes. In yeast, CF IA and CPF constitute the core 3'-end maturation complex. CF IA comprises Rna14p, Rna15p, Pcf11p and Clp1p. CF IA interacts with the C-terminal domain of RNA Pol II largest subunit via Pcf11p which links pre-mRNA 3'-...

journal_title：Nucleic acids research

authors： Haddad R,Maurice F,Viphakone N,Voisinet-Hakil F,Fribourg S,Minvielle-Sébastia L

更新日期：2012-02-01 00:00:00

abstract：:Primer Design Assistant (PDA) is a web interface primer design service combined with thermodynamic theory to evaluate the fitness of primers. It runs in a Linux-Apache-MySQL-PHP structure on a PC equipped with dual CPU (Intel Pentium III 1.4 GHz) and 512 Mb of RAM. A succinct user interface of PDA is accomplished by b...

journal_title：Nucleic acids research

authors： Chen SH,Lin CY,Cho CS,Lo CZ,Hsiung CA

更新日期：2003-07-01 00:00:00

abstract：:Next-generation DNA sequencing platforms provide exciting new possibilities for in vitro genetic analysis of functional nucleic acids. However, the size of the resulting data sets presents computational and analytical challenges. We present an open-source software package that employs a locality-sensitive hashing algo...

journal_title：Nucleic acids research

authors： Pitt JN,Rajapakse I,Ferré-D'Amaré AR

更新日期：2010-12-01 00:00:00

abstract：:The wobble uridine of certain bacterial and mitochondrial tRNAs is modified, at position 5, through an unknown reaction pathway that utilizes the evolutionarily conserved MnmE and GidA proteins. The resulting modification (a methyluridine derivative) plays a critical role in decoding NNG/A codons and reading frame mai...

journal_title：Nucleic acids research

authors： Moukadiri I,Prado S,Piera J,Velázquez-Campoy A,Björk GR,Armengod ME

更新日期：2009-11-01 00:00:00

abstract：:Sequence-specific DNA-binding proteins play a key role in many fundamental biological processes, such as transcription, DNA replication and recombination. Very often, these DNA-binding proteins introduce structural changes to the target DNA-binding sites including DNA bending, twisting or untwisting and wrapping, whic...

journal_title：Nucleic acids research

authors： Chen B,Xiao Y,Liu C,Li C,Leng F

更新日期：2010-06-01 00:00:00

abstract：:We previously showed that mRNAs synthesized from three genes that naturally lack introns contain a portion of their coding sequence, known as a cytoplasmic accumulation region (CAR), which is essential for stable accumulation of the intronless mRNAs in the cytoplasm. The CAR in each mRNA is unexpectedly large, ranging...

journal_title：Nucleic acids research

authors： Lei H,Zhai B,Yin S,Gygi S,Reed R

更新日期：2013-02-01 00:00:00

abstract：:The Yeast Protein Localization database (YPL.db) contains information about the localization patterns of yeast proteins resulting from microscopic analyses. The data and parameters of the experiments to obtain the localization information, together with images from confocal or video microscopy, are stored in a relatio...

journal_title：Nucleic acids research

authors： Habeler G,Natter K,Thallinger GG,Crawford ME,Kohlwein SD,Trajanoski Z

更新日期：2002-01-01 00:00:00

abstract：:Hfq, a bacterial RNA-binding protein, was recently shown to contain the Sm1 motif, a characteristic of Sm and LSm proteins that function in RNA processing events in archaea and eukaryotes. In this report, comparative structural modeling was used to predict a three-dimensional structure of the Hfq core sequence. The pr...

journal_title：Nucleic acids research

authors： Sun X,Zhulin I,Wartell RM

更新日期：2002-09-01 00:00:00

abstract：:Melibiose uptake and hydrolysis in E.coli is performed by the MelB and MelA proteins, respectively. We report the cloning and sequencing of the melA gene. The nucleotide sequence data showed that melA codes for a 450 amino acid long protein with a molecular weight of 50.6 kd. The sequence data also supported the assum...

journal_title：Nucleic acids research

authors： Liljeström PL,Liljeström P

更新日期：1987-03-11 00:00:00

abstract：:Effective transcript profiling in animal systems requires isolation of homogenous tissue or cells followed by faithful mRNA amplification. Linear amplification based on cDNA synthesis and in vitro transcription is reported to maintain representation of mRNA levels, however, quantitative data demonstrating this as well...

journal_title：Nucleic acids research

authors： Baugh LR,Hill AA,Brown EL,Hunter CP

更新日期：2001-03-01 00:00:00

abstract：:Phospho3D is a database of three-dimensional (3D) structures of phosphorylation sites (P-sites) derived from the Phospho.ELM database, which also collects information on the residues surrounding the P-site in space (3D zones). The database also provides the results of a large-scale structural comparison of the 3D zone...

journal_title：Nucleic acids research

authors： Zanzoni A,Carbajo D,Diella F,Gherardini PF,Tramontano A,Helmer-Citterich M,Via A

更新日期：2011-01-01 00:00:00

abstract：:DNA repair is required to maintain genome stability in stem cells and early embryos. At critical junctures, oxidative damage to DNA requires the base excision repair (BER) pathway. Since early zebrafish embryos lack the major polymerase in BER, DNA polymerase ß, repair proceeds via replicative polymerases, even though...

journal_title：Nucleic acids research

authors： Pei DS,Yang XJ,Liu W,Guikema JE,Schrader CE,Strauss PR

更新日期：2011-04-01 00:00:00

abstract：:Splicing is a biological phenomenon that removes the non-coding sequence from the transcripts to produce a mature transcript suitable for translation. To study this phenomenon, information on the intron-exon arrangement of a gene is essential, usually obtained by aligning mRNA/EST sequences to their cognate genomic se...

journal_title：Nucleic acids research

authors： Lee BT,Tan TW,Ranganathan S

更新日期：2003-07-01 00:00:00

abstract：:On the tandemly linked ribosomal genes of Xenopus laevis, the RNA polymerase transcribes past the 3' end of the 40S coding region and terminates at T3 just upstream of the gene promoter. The close proximity of T3 to the gene promoter, and the functional interdependence of these two elements, has led to the suggestion ...

journal_title：Nucleic acids research

authors： Lucchini R,Reeder RH

更新日期：1989-01-11 00:00:00

abstract：:Post-translational modifications (PTMs) play a key role in numerous cellular processes by directly affecting structure, dynamics and interaction networks of target proteins. Despite their importance, our understanding of protein PTMs at the atomistic level is still largely incomplete. Molecular dynamics (MD) simulatio...

journal_title：Nucleic acids research

authors： Margreitter C,Petrov D,Zagrovic B

更新日期：2013-07-01 00:00:00