Abstract:
:Transcriptome profiling is widely used to infer functional states of specific cell types, as well as their responses to stimuli, to define contributions to physiology and pathophysiology. Focusing on microglia, the brain's macrophages, we report here a side-by-side comparison of classical cell-sorting-based transcriptome sequencing and the 'RiboTag' method, which avoids cell retrieval from tissue context and yields translatome sequencing information. Conventional whole-cell microglial transcriptomes were found to be significantly tainted by artifacts introduced by tissue dissociation, cargo contamination and transcripts sequestered from ribosomes. Conversely, our data highlight the added value of RiboTag profiling for assessing the lineage accuracy of Cre recombinase expression in transgenic mice. Collectively, this study indicates method-based biases, reveals observer effects and establishes RiboTag-based translatome profiling as a valuable complement to standard sorting-based profiling strategies.
journal_name
Nat Immunoljournal_title
Nature immunologyauthors
Haimon Z,Volaski A,Orthgiess J,Boura-Halfon S,Varol D,Shemer A,Yona S,Zuckerman B,David E,Chappell-Maor L,Bechmann I,Gericke M,Ulitsky I,Jung Sdoi
10.1038/s41590-018-0110-6subject
Has Abstractpub_date
2018-06-01 00:00:00pages
636-644issue
6eissn
1529-2908issn
1529-2916pii
10.1038/s41590-018-0110-6journal_volume
19pub_type
杂志文章abstract::The maintenance of immunological tolerance requires the deletion of self-reactive T cells in the thymus. The expression of genes encoding tissue-specific antigens (TSAs) by thymic epithelial cells is critical for this process and depends on activity of the transcriptional regulator Aire; however, the molecular mechani...
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abstract:: ...
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