Abstract：
:Odontoblasts, with their strategic arrangement along the outermost compartment of the dentin-pulp complex, have been suggested to have sensory function. In addition to their primary role in dentin formation, growing evidence shows that odontoblasts are capable of sensing mechanical stimulation. Previously, we found that most odontoblasts express TRPM7, the nonselective mechanosensitive ion channel reported to be critical in Mg2+ homeostasis and dentin mineralization. In line with this finding, we sought to elucidate the functional expression of TRPM7 in odontoblasts by pharmacological approaches and mechanical stimulation. Naltriben, a TRPM7-specific agonist, induced calcium transient in the majority of odontoblasts, which was blocked by TRPM7 blockers such as extracellular Mg2+ and FTY720 in a dose-dependent manner. Mechanical stretch of the odontoblastic membrane with hypotonic solution also induced calcium transient, which was blocked by Gd3+, a nonselective mechanosensitive channel blocker. Calcium transient induced by hypotonic solution was also blocked by high extracellular Mg2+ or FTY720. When TRPM7-mediated calcium transients in odontoblasts were analyzed on the subcellular level, remarkably larger transients were detected in the distal odontoblastic process compared with the soma, which was further verified with comparable immunocytochemical analysis. Our results demonstrate that TRPM7 in odontoblasts can serve as a mechanical sensor, with its distribution to facilitate intracellular Ca2+ signaling in the odontoblastic process. These findings suggest TRPM7 as a mechanical transducer in odontoblasts to mediate intracellular calcium dynamics under diverse pathophysiological conditions of the dentin.
journal_name
J Dent Resjournal_title
Journal of dental researchauthors
Won J,Vang H,Kim JH,Lee PR,Kang Y,Oh SBdoi
10.1177/0022034518759947subject
Has Abstractpub_date
2018-08-01 00:00:00pages
1039-1046issue
9eissn
0022-0345issn
1544-0591journal_volume
97pub_type
杂志文章abstract：:The extent of dental tissue destruction during the treatment of white spot lesions (WSLs) increases with the severity of the lesion. If the depth and shape of WSLs can be predicted with a noninvasive diagnostic method before dental caries treatment, more conservative interventions can be planned. Given the superiority...
journal_title：Journal of dental research
pub_type： 杂志文章
doi：10.1177/0022034518811642
更新日期：2019-03-01 00:00:00
abstract：:Degradation of dentin matrix components within caries dentin has been correlated with the activity of host-derived proteases, such as matrix metalloproteases (MMPs) and cysteine cathepsins (CTs). Since this relationship has not been fully established, we hypothesized that the abundance of MMPs and CTs in caries-affect...
journal_title：Journal of dental research
pub_type： 杂志文章
doi：10.1177/0022034513516979
更新日期：2014-03-01 00:00:00
abstract：:Fluoride content of drinking water is an important criterion in prescribing fluoride supplements. The majority of practicing dentists and physicians prescribe fluoride supplements, yet many apparently are unaware of the fluoride levels in their patients' drinking water. Characteristics of North Carolina (NC) dentists,...
journal_title：Journal of dental research
pub_type： 杂志文章
doi：10.1177/00220345860650011301
更新日期：1986-01-01 00:00:00
abstract：:Samples of human dental plaque were pooled from several "white spot" smooth tooth surface areas as well as from several clinically-sound tooth surface areas in each of 12 caries-positive college students. Each of the two samples from each subject was used for the determination of: (1) pH-lowering potential in vitro in...
journal_title：Journal of dental research
pub_type： 杂志文章
doi：10.1177/00220345910700120501
更新日期：1991-12-01 00:00:00
abstract：:Studies on mechanisms underlying the differentiation of dental pulp stem cells are critical for the understanding of the biology of odontogenesis and for dental tissue engineering. Here, we tested the hypothesis that stem cells from exfoliated deciduous teeth (SHED) differentiate into functional odontoblasts and endot...
journal_title：Journal of dental research
pub_type： 杂志文章
doi：10.1177/0022034510368647
更新日期：2010-08-01 00:00:00
abstract：:Strength and durability of the dentin-acrylic resin joint cemented with 2-cyanoacrylate esters were studied. Maximum adhesion is obtained with isobutyl 2-cyanoacrylate after 1% acid pretreatment of the dentin. Hydrolytic stability is somewhat improved by addition of polymer to the adhesive or coating around the joint....
journal_title：Journal of dental research
pub_type： 杂志文章
doi：10.1177/00220345790580090901
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abstract：:An adhesive opaque resin for veneering on cast metal was developed with 4-methacryloxyethyl trimellitate anhydride and poly (methyl methacrylate)-coated titanium dioxide prepared by aqueous phase polymerization. The opaque resin was a modified 4-META/MMA-TBB resin. The powder consisted of 20% of the encapsulated mater...
journal_title：Journal of dental research
pub_type： 杂志文章
doi：10.1177/00220345880670010501
更新日期：1988-01-01 00:00:00
abstract：:Juvenile rheumatoid arthritis (JRA) is a chronic systemic disease of childhood that affects synovial joints including the temporomandibular joint (TMJ). Individuals with JRA of the TMJ frequently show aberrations in mandibulofacial development. Since the basis for these developmental perturbations is poorly understood...
journal_title：Journal of dental research
pub_type： 杂志文章
doi：10.1177/00220345990780080801
更新日期：1999-08-01 00:00:00
abstract：:This study was conducted to test the hypothesis that physiological changes which occur during aging increase the biological impact of fluoride and reduce the threshold of safe fluoride exposure. Four groups of rats were fed a low-fluoride diet (< 1.2 ppm) ad libitum and received 0, 5, 15, or 50 ppm fluoride in their d...
journal_title：Journal of dental research
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doi：10.1177/00220345950740011201
更新日期：1995-01-01 00:00:00
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journal_title：Journal of dental research
pub_type： 杂志文章,评审
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更新日期：1992-07-01 00:00:00
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journal_title：Journal of dental research
pub_type： 杂志文章
doi：10.1177/0022034509334499
更新日期：2009-04-01 00:00:00
abstract：:Current knowledge regarding the sensitivity of the teeth to forces is based on psychophysical experiments that measured touch detection thresholds under static jaw conditions. It is not known whether jaw movements alter the perception of forces applied to the teeth, but, based on limb movement studies, it is hypothesi...
journal_title：Journal of dental research
pub_type： 杂志文章
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更新日期：2010-04-01 00:00:00
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journal_title：Journal of dental research
pub_type： 杂志文章
doi：10.1177/0022034516653085
更新日期：2016-10-01 00:00:00
abstract：:Non-steroidal anti-inflammatory drugs (NSAIDs) are used to manage pain and inflammatory disorders. We hypothesized that gingival fibroblasts actively accumulate NSAIDs and enhance their levels in gingival connective tissue. Using fluorescence to monitor NSAID transport, we demonstrated that cultured gingival fibroblas...
journal_title：Journal of dental research
pub_type： 杂志文章
doi：10.1177/154405910608500511
更新日期：2006-05-01 00:00:00
abstract：:The human patched gene (PTCH) functions in both embryologic development and tumor suppression. PTCH mutations have been found in odontogenic keratocysts. However, the expression and localization of the protein product of the gene have not been determined in odontogenic tumors and cysts. We investigated 68 odontogenic ...
journal_title：Journal of dental research
pub_type： 杂志文章
doi：10.1177/0810757
更新日期：2002-11-01 00:00:00
abstract：:Submandibular glands from young adult and aged male and female rats have been examined and various descriptive parameters obtained. Several sex differences were observed with tissue from young and old animals. Sex differences were found in the gland contents of DNA, protein, and sialic acid and in some protein compone...
journal_title：Journal of dental research
pub_type： 杂志文章
doi：10.1177/00220345810600051501
更新日期：1981-05-01 00:00:00
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journal_title：Journal of dental research
pub_type： 杂志文章
doi：10.1177/0022034517707512
更新日期：2017-07-01 00:00:00
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journal_title：Journal of dental research
pub_type： 杂志文章,评审
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更新日期：2019-02-01 00:00:00
abstract：:Patients suffering from temporomandibular dysfunction exhibit clinical findings such as fatigue, painful muscles, and muscles that are tender to palpation, not only in the temporomandibular area, but also in the neck/shoulder region. The mechanisms behind this are not known, although previous studies of similar spread...
journal_title：Journal of dental research
pub_type： 杂志文章
doi：10.1177/00220345000790101401
更新日期：2000-10-01 00:00:00
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journal_title：Journal of dental research
pub_type： 杂志文章,评审
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更新日期：2013-07-01 00:00:00
abstract：:The oral cavity contains a rich consortium of exopolysaccharide-producing microbes. These extracellular polysaccharides comprise a major component of the oral biofilm. Together with extracellular proteins, DNA, and lipids, they form the biofilm matrix, which contributes to bacterial colonization, biofilm formation and...
journal_title：Journal of dental research
pub_type： 杂志文章,评审
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更新日期：2019-07-01 00:00:00
abstract：:The human dentition is a complex adaptive system that is influenced by genetic, epigenetic, and environmental factors. Within this system, is sexual dimorphism related to the growth promotion of the Y chromosome, or to hormonal influences, or both? This study is the first to investigate both primary and permanent toot...
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journal_title：Journal of dental research
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更新日期：2016-04-01 00:00:00
abstract：:Porcine dentin sialophosphoprotein (DSPP) is the most abundant non-collagenous protein in dentin. It is processed by proteases into 3 independent proteins: dentin sialoprotein (DSP), dentin glycoprotein (DGP), and dentin phosphoprotein (DPP). We fractionated DPP and DSP along with TGF-β activity by ion exchange (IE) c...
journal_title：Journal of dental research
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abstract：:Fluid transudation through simplified dentin adhesives can occur in bonded vital crown dentin, since these adhesives behave as permeable membranes after polymerization. The effect of adhesive permeability in endodontically treated teeth is unknown. This study examined the hypothesis that in vivo fluid movement through...
journal_title：Journal of dental research
pub_type： 临床试验,杂志文章,随机对照试验
doi：10.1177/154405910508400303
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abstract：:Growing interest in the treatment and prevention of Molar/Incisor Hypomineralization (MIH) warrants investigation into the protein composition of hypomineralized enamel. Hypothesizing abnormality akin to amelogenesis imperfecta, we profiled proteins in hypomineralized enamel from human permanent first molars using a b...
journal_title：Journal of dental research
pub_type： 杂志文章
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更新日期：2010-10-01 00:00:00
abstract：:Streptococcus mutans produces 3 types of glucosyltransferase (GTF), whose cooperative action is considered to be essential for its cellular adherence to the tooth surface. However, the precise mechanisms for synthesizing adhesive glucans and the specific roles of each GTF in cellular adherence to smooth surfaces have ...
journal_title：Journal of dental research
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doi：10.1177/00220345010800071401
更新日期：2001-07-01 00:00:00
abstract：:At the present time, peri-implantitis has become a global burden that occurs with a frequency from 1% to 47% at implant level. Therefore, we aimed herein at assessing the impact of peri-implant maintenance therapy (PIMT) on the prevention of peri-implant diseases. Electronic and manual literature searches were conduct...
journal_title：Journal of dental research
pub_type： 杂志文章,meta分析,评审
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abstract：:The purpose of the study was to determine the exposure times needed to harden a light-activated composite cement through various thicknesses of porcelain. A negative exponential model was developed with which the relative hardness of a composite could be predicted from the product of the exposure time and the square r...
journal_title：Journal of dental research
pub_type： 杂志文章
doi：10.1177/00220345890680030801
更新日期：1989-03-01 00:00:00
abstract：:The objective of this study was to calculate the stress distribution induced in anterior metal-ceramic crowns fabricated with either gold-alloy or nickel-alloy copings of reduced thickness using plane stress analyses. Two-dimensional finite element models of three crown designs were subjected to a simulated biting for...
journal_title：Journal of dental research
pub_type： 杂志文章
doi：10.1177/00220345860650091201
更新日期：1986-09-01 00:00:00
