Abstract：
:A longstanding goal of synthetic biology has been the programmable control of cellular functions. Central to this is the creation of versatile regulatory toolsets that allow for programmable control of gene expression. Of the many regulatory molecules available, RNA regulators offer the intriguing possibility of de novo design-allowing for the bottom-up molecular-level design of genetic control systems. Here we present a computational design approach for the creation of a bacterial regulator called Small Transcription Activating RNAs (STARs) and create a library of high-performing and orthogonal STARs that achieve up to ~ 9000-fold gene activation. We demonstrate the versatility of these STARs-from acting synergistically with existing constitutive and inducible regulators, to reprogramming cellular phenotypes and controlling multigene metabolic pathway expression. Finally, we combine these new STARs with themselves and CRISPRi transcriptional repressors to deliver new types of RNA-based genetic circuitry that allow for sophisticated and temporal control of gene expression.
journal_name
Nat Communjournal_title
Nature communicationsauthors
Chappell J,Westbrook A,Verosloff M,Lucks JBdoi
10.1038/s41467-017-01082-6subject
Has Abstractpub_date
2017-10-19 00:00:00pages
1051issue
1issn
2041-1723pii
10.1038/s41467-017-01082-6journal_volume
8pub_type
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