Biotransformation of p-xylene into terephthalic acid by engineered Escherichia coli.

Abstract:

:Terephthalic acid (TPA) is an important industrial chemical currently produced by energy intensive and potentially hazardous p-xylene (pX) oxidation process. Here we report the development of metabolically engineered Escherichia coli system for biological transformation of pX into TPA. The engineered E. coli strain harbours a synthetic TPA pathway optimized through manipulation of expression levels of upstream and downstream modules. The upstream pathway converts pX to p-toluic acid (pTA) and the downstream pathway transforms pTA to TPA. In a two-phase partitioning fermentation, the engineered strain converts 8.8 g pX into 13.3 g TPA, which corresponds to a conversion yield of 96.7 mol%. These results suggest that the E. coli system presented here might be a promising alternative for the large-scale biotechnological production of TPA and lays the foundations for the future development of sustainable approaches for TPA production.

journal_name

Nat Commun

journal_title

Nature communications

authors

Luo ZW,Lee SY

doi

10.1038/ncomms15689

subject

Has Abstract

pub_date

2017-05-31 00:00:00

pages

15689

issn

2041-1723

pii

ncomms15689

journal_volume

8

pub_type

杂志文章
  • Author Correction: Large intrinsic anomalous Hall effect in half-metallic ferromagnet Co3Sn2S2 with magnetic Weyl fermions.

    abstract::The original version of this Article incorrectly omitted an affiliation of Hongming Weng: "Beijing National Laboratory for Condensed Matter Physics and Institute of Physics, Chinese Academy of Sciences, 100190, Beijing, China"This has been corrected in both the PDF and HTML versions of the Article. ...

    journal_title:Nature communications

    pub_type: 杂志文章,已发布勘误

    doi:10.1038/s41467-018-06643-x

    authors: Wang Q,Xu Y,Lou R,Liu Z,Li M,Huang Y,Shen D,Weng H,Wang S,Lei H

    更新日期:2018-10-08 00:00:00

  • Electrochemically tunable thermal conductivity of lithium cobalt oxide.

    abstract::Using time-domain thermoreflectance, the thermal conductivity and elastic properties of a sputter deposited LiCoO2 film, a common lithium-ion cathode material, are measured as a function of the degree of lithiation. Here we report that via in situ measurements during cycling, the thermal conductivity of a LiCoO2 catho...

    journal_title:Nature communications

    pub_type: 杂志文章

    doi:10.1038/ncomms5035

    authors: Cho J,Losego MD,Zhang HG,Kim H,Zuo J,Petrov I,Cahill DG,Braun PV

    更新日期:2014-06-03 00:00:00

  • GFI1 facilitates efficient DNA repair by regulating PRMT1 dependent methylation of MRE11 and 53BP1.

    abstract::GFI1 is a transcriptional regulator expressed in lymphoid cells, and an "oncorequisite" factor required for development and maintenance of T-lymphoid leukemia. GFI1 deletion causes hypersensitivity to ionizing radiation, for which the molecular mechanism remains unknown. Here, we demonstrate that GFI1 is required in T...

    journal_title:Nature communications

    pub_type: 杂志文章

    doi:10.1038/s41467-018-03817-5

    authors: Vadnais C,Chen R,Fraszczak J,Yu Z,Boulais J,Pinder J,Frank D,Khandanpour C,Hébert J,Dellaire G,Côté JF,Richard S,Orthwein A,Drobetsky E,Möröy T

    更新日期:2018-04-12 00:00:00

  • A unified neurocognitive model of semantics language social behaviour and face recognition in semantic dementia.

    abstract::The anterior temporal lobes (ATL) have become a key brain region of interest in cognitive neuroscience founded upon neuropsychological investigations of semantic dementia (SD). The purposes of this investigation are to generate a single unified model that captures the known cognitive-behavioural variations in SD and m...

    journal_title:Nature communications

    pub_type: 杂志文章

    doi:10.1038/s41467-020-16089-9

    authors: Ding J,Chen K,Liu H,Huang L,Chen Y,Lv Y,Yang Q,Guo Q,Han Z,Lambon Ralph MA

    更新日期:2020-05-22 00:00:00

  • Distinct submembrane localisation compartmentalises cardiac NPR1 and NPR2 signalling to cGMP.

    abstract::Natriuretic peptides (NPs) are important hormones that regulate multiple cellular functions including cardiovascular physiology. In the heart, two natriuretic peptide receptors NPR1 and NPR2 act as membrane guanylyl cyclases to produce 3',5'-cyclic guanosine monophosphate (cGMP). Although both receptors protect from c...

    journal_title:Nature communications

    pub_type: 杂志文章

    doi:10.1038/s41467-018-04891-5

    authors: Subramanian H,Froese A,Jönsson P,Schmidt H,Gorelik J,Nikolaev VO

    更新日期:2018-06-22 00:00:00

  • Compositional boundary layers trigger liquid unmixing in a basaltic crystal mush.

    abstract::The separation of immiscible liquids has significant implications for magma evolution and the formation of magmatic ore deposits. We combine high-resolution imaging and electron probe microanalysis with the first use of atom probe tomography on tholeiitic basaltic glass from Hawaii, the Snake River Plain, and Iceland,...

    journal_title:Nature communications

    pub_type: 杂志文章

    doi:10.1038/s41467-019-12694-5

    authors: Honour VC,Holness MB,Charlier B,Piazolo SC,Namur O,Prosa TJ,Martin I,Helz RT,Maclennan J,Jean MM

    更新日期:2019-10-23 00:00:00

  • Nanofabrication for all-soft and high-density electronic devices based on liquid metal.

    abstract::Innovations in soft material synthesis and fabrication technologies have led to the development of integrated soft electronic devices. Such soft devices offer opportunities to interact with biological cells, mimicking their soft environment. However, existing fabrication technologies cannot create the submicron-scale,...

    journal_title:Nature communications

    pub_type: 杂志文章

    doi:10.1038/s41467-020-14814-y

    authors: Kim MG,Brown DK,Brand O

    更新日期:2020-02-21 00:00:00

  • Corrigendum: Muscle-specific CRISPR/Cas9 dystrophin gene editing ameliorates pathophysiology in a mouse model for Duchenne muscular dystrophy.

    abstract::This corrects the article DOI: 10.1038/ncomms14454. ...

    journal_title:Nature communications

    pub_type: 杂志文章,已发布勘误

    doi:10.1038/ncomms16007

    authors: Bengtsson NE,Hall JK,Odom GL,Phelps MP,Andrus CR,Hawkins RD,Hauschka SD,Chamberlain JR,Chamberlain JS

    更新日期:2017-06-23 00:00:00

  • Publisher Correction: Orbital bistatic radar observations of asteroid Vesta by the Dawn mission.

    abstract::An incorrect version of the Supplementary Information was inadvertently published with this Article. The HTML has now been updated to include the correct version of the Supplementary Information. ...

    journal_title:Nature communications

    pub_type: 杂志文章,已发布勘误

    doi:10.1038/s41467-017-01617-x

    authors: Palmer EM,Heggy E,Kofman W

    更新日期:2017-11-23 00:00:00

  • Reliable detection of subclonal single-nucleotide variants in tumour cell populations.

    abstract::According to the clonal evolution model, tumour growth is driven by competing subclones in somatically evolving cancer cell populations, which gives rise to genetically heterogeneous tumours. Here we present a comparative targeted deep-sequencing approach combined with a customised statistical algorithm, called deepSN...

    journal_title:Nature communications

    pub_type: 杂志文章

    doi:10.1038/ncomms1814

    authors: Gerstung M,Beisel C,Rechsteiner M,Wild P,Schraml P,Moch H,Beerenwinkel N

    更新日期:2012-05-01 00:00:00

  • Structure of Schlafen13 reveals a new class of tRNA/rRNA- targeting RNase engaged in translational control.

    abstract::Cleavage of transfer (t)RNA and ribosomal (r)RNA are critical and conserved steps of translational control for cells to overcome varied environmental stresses. However, enzymes that are responsible for this event have not been fully identified in high eukaryotes. Here, we report a mammalian tRNA/rRNA-targeting endorib...

    journal_title:Nature communications

    pub_type: 杂志文章

    doi:10.1038/s41467-018-03544-x

    authors: Yang JY,Deng XY,Li YS,Ma XC,Feng JX,Yu B,Chen Y,Luo YL,Wang X,Chen ML,Fang ZX,Zheng FX,Li YP,Zhong Q,Kang TB,Song LB,Xu RH,Zeng MS,Chen W,Zhang H,Xie W,Gao S

    更新日期:2018-03-21 00:00:00

  • Electron hydrodynamics in anisotropic materials.

    abstract::Rotational invariance strongly constrains the viscosity tensor of classical fluids. When this symmetry is broken in anisotropic materials a wide array of novel phenomena become possible. We explore electron fluid behaviors arising from the most general viscosity tensors in two and three dimensions, constrained only th...

    journal_title:Nature communications

    pub_type: 杂志文章

    doi:10.1038/s41467-020-18553-y

    authors: Varnavides G,Jermyn AS,Anikeeva P,Felser C,Narang P

    更新日期:2020-09-18 00:00:00

  • Nitric oxide-dependent anaerobic ammonium oxidation.

    abstract::Nitric oxide (NO) has important functions in biology and atmospheric chemistry as a toxin, signaling molecule, ozone depleting agent and the precursor of the greenhouse gas nitrous oxide (N2O). Although NO is a potent oxidant, and was available on Earth earlier than oxygen, it is unclear whether NO can be used by micr...

    journal_title:Nature communications

    pub_type: 杂志文章

    doi:10.1038/s41467-019-09268-w

    authors: Hu Z,Wessels HJCT,van Alen T,Jetten MSM,Kartal B

    更新日期:2019-03-20 00:00:00

  • Confidence controls perceptual evidence accumulation.

    abstract::Perceptual decisions are accompanied by feelings of confidence that reflect the likelihood that the decision was correct. Here we aim to clarify the relationship between perception and confidence by studying the same perceptual task across three different confidence contexts. Human observers were asked to categorize t...

    journal_title:Nature communications

    pub_type: 杂志文章

    doi:10.1038/s41467-020-15561-w

    authors: Balsdon T,Wyart V,Mamassian P

    更新日期:2020-04-09 00:00:00

  • Telomerase reverse transcriptase promoter mutations in primary cutaneous melanoma.

    abstract::We previously reported a disease segregating causal germline mutation in a melanoma family and recurrent somatic mutations in metastasized tumours from unrelated patients in the core promoter region of the telomerase reverse transcriptase (TERT) gene. Here we show that the TERT promoter mutations, besides causing an i...

    journal_title:Nature communications

    pub_type: 杂志文章

    doi:10.1038/ncomms4401

    authors: Heidenreich B,Nagore E,Rachakonda PS,Garcia-Casado Z,Requena C,Traves V,Becker J,Soufir N,Hemminki K,Kumar R

    更新日期:2014-02-26 00:00:00

  • A clustering-independent method for finding differentially expressed genes in single-cell transcriptome data.

    abstract::A common analysis of single-cell sequencing data includes clustering of cells and identifying differentially expressed genes (DEGs). How cell clusters are defined has important consequences for downstream analyses and the interpretation of results, but is often not straightforward. To address this difficulty, we prese...

    journal_title:Nature communications

    pub_type: 杂志文章

    doi:10.1038/s41467-020-17900-3

    authors: Vandenbon A,Diez D

    更新日期:2020-08-28 00:00:00

  • Weaker plant-enemy interactions decrease tree seedling diversity with edge-effects in a fragmented tropical forest.

    abstract::In fragmented forests, tree diversity declines near edges but the ecological processes underlying this loss of diversity remain poorly understood. Theory predicts that top-down regulation of seedling recruitment by insect herbivores and fungal pathogens contributes to maintaining tree diversity in forests, but it is u...

    journal_title:Nature communications

    pub_type: 杂志文章

    doi:10.1038/s41467-018-06997-2

    authors: Krishnadas M,Bagchi R,Sridhara S,Comita LS

    更新日期:2018-10-30 00:00:00

  • Corrigendum: Superfast assembly and synthesis of gold nanostructures using nanosecond low-temperature compression via magnetic pulsed power.

    abstract::This corrects the article DOI: 10.1038/ncomms14778. ...

    journal_title:Nature communications

    pub_type: 杂志文章,已发布勘误

    doi:10.1038/ncomms15574

    authors: Li B,Bian K,Lane JMD,Salerno KM,Grest GS,Ao T,Hickman R,Wise J,Wang Z,Fan H

    更新日期:2017-05-24 00:00:00

  • Vulnerability of progeroid smooth muscle cells to biomechanical forces is mediated by MMP13.

    abstract::Hutchinson-Gilford Progeria Syndrome (HGPS) is a premature aging disease in children that leads to early death. Smooth muscle cells (SMCs) are the most affected cells in HGPS individuals, although the reason for such vulnerability remains poorly understood. In this work, we develop a microfluidic chip formed by HGPS-S...

    journal_title:Nature communications

    pub_type: 杂志文章

    doi:10.1038/s41467-020-17901-2

    authors: Pitrez PR,Estronca L,Monteiro LM,Colell G,Vazão H,Santinha D,Harhouri K,Thornton D,Navarro C,Egesipe AL,Carvalho T,Dos Santos RL,Lévy N,Smith JC,de Magalhães JP,Ori A,Bernardo A,De Sandre-Giovannoli A,Nissan X,Rosel

    更新日期:2020-08-17 00:00:00

  • Intron retention is regulated by altered MeCP2-mediated splicing factor recruitment.

    abstract::While intron retention (IR) is considered a widely conserved and distinct mechanism of gene expression control, its regulation is poorly understood. Here we show that DNA methylation directly regulates IR. We also find reduced occupancy of MeCP2 near the splice junctions of retained introns, mirroring the reduced DNA ...

    journal_title:Nature communications

    pub_type: 杂志文章

    doi:10.1038/ncomms15134

    authors: Wong JJ,Gao D,Nguyen TV,Kwok CT,van Geldermalsen M,Middleton R,Pinello N,Thoeng A,Nagarajah R,Holst J,Ritchie W,Rasko JEJ

    更新日期:2017-05-08 00:00:00

  • Inflammation-induced proteolytic processing of the SIRPα cytoplasmic ITIM in neutrophils propagates a proinflammatory state.

    abstract::Signal regulatory protein α (SIRPα), an immunoreceptor tyrosine-based inhibitory motif (ITIM)-containing receptor, is an essential negative regulator of leukocyte inflammatory responses. Here we report that SIRPα cytoplasmic signalling ITIMs in neutrophils are cleaved during active inflammation and that the loss of SI...

    journal_title:Nature communications

    pub_type: 杂志文章

    doi:10.1038/ncomms3436

    authors: Zen K,Guo Y,Bian Z,Lv Z,Zhu D,Ohnishi H,Matozaki T,Liu Y

    更新日期:2013-01-01 00:00:00

  • Metallo-polyelectrolytes as a class of ionic macromolecules for functional materials.

    abstract::The fields of soft polymers and macromolecular sciences have enjoyed a unique combination of metals and organic frameworks in the name of metallopolymers or organometallic polymers. When metallopolymers carry charged groups, they form a class of metal-containing polyelectrolytes or metallo-polyelectrolytes. This revie...

    journal_title:Nature communications

    pub_type: 杂志文章,评审

    doi:10.1038/s41467-018-06475-9

    authors: Zhu T,Sha Y,Yan J,Pageni P,Rahman MA,Yan Y,Tang C

    更新日期:2018-10-18 00:00:00

  • A molecular gradient along the longitudinal axis of the human hippocampus informs large-scale behavioral systems.

    abstract::The functional organization of the hippocampus is distributed as a gradient along its longitudinal axis that explains its differential interaction with diverse brain systems. We show that the location of human tissue samples extracted along the longitudinal axis of the adult human hippocampus can be predicted within 2...

    journal_title:Nature communications

    pub_type: 杂志文章

    doi:10.1038/s41467-020-14518-3

    authors: Vogel JW,La Joie R,Grothe MJ,Diaz-Papkovich A,Doyle A,Vachon-Presseau E,Lepage C,Vos de Wael R,Thomas RA,Iturria-Medina Y,Bernhardt B,Rabinovici GD,Evans AC

    更新日期:2020-02-19 00:00:00

  • Layer- and cell type-selective co-transmission by a basal forebrain cholinergic projection to the olfactory bulb.

    abstract::Cholinergic neurons in the basal forebrain project heavily to the main olfactory bulb, the first processing station in the olfactory pathway. The projections innervate multiple layers of the main olfactory bulb and strongly influence odor discrimination, detection, and learning. The precise underlying circuitry of thi...

    journal_title:Nature communications

    pub_type: 杂志文章

    doi:10.1038/s41467-017-00765-4

    authors: Case DT,Burton SD,Gedeon JY,Williams SG,Urban NN,Seal RP

    更新日期:2017-09-21 00:00:00

  • Lattice engineering enables definition of molecular features allowing for potent small-molecule inhibition of HIV-1 entry.

    abstract::Diverse entry inhibitors targeting the gp120 subunit of the HIV-1 envelope (Env) trimer have been developed including BMS-626529, also called temsavir, a prodrug version of which is currently in phase III clinical trials. Here we report the characterization of a panel of small-molecule inhibitors including BMS-818251,...

    journal_title:Nature communications

    pub_type: 杂志文章

    doi:10.1038/s41467-018-07851-1

    authors: Lai YT,Wang T,O'Dell S,Louder MK,Schön A,Cheung CSF,Chuang GY,Druz A,Lin B,McKee K,Peng D,Yang Y,Zhang B,Herschhorn A,Sodroski J,Bailer RT,Doria-Rose NA,Mascola JR,Langley DR,Kwong PD

    更新日期:2019-01-03 00:00:00

  • Autonomous model protocell division driven by molecular replication.

    abstract::The coupling of compartmentalisation with molecular replication is thought to be crucial for the emergence of the first evolvable chemical systems. Minimal artificial replicators have been designed based on molecular recognition, inspired by the template copying of DNA, but none yet have been coupled to compartmentali...

    journal_title:Nature communications

    pub_type: 杂志文章

    doi:10.1038/s41467-017-00177-4

    authors: Taylor JW,Eghtesadi SA,Points LJ,Liu T,Cronin L

    更新日期:2017-08-10 00:00:00

  • Genome of The Netherlands population-specific imputations identify an ABCA6 variant associated with cholesterol levels.

    abstract::Variants associated with blood lipid levels may be population-specific. To identify low-frequency variants associated with this phenotype, population-specific reference panels may be used. Here we impute nine large Dutch biobanks (~35,000 samples) with the population-specific reference panel created by the Genome of T...

    journal_title:Nature communications

    pub_type: 杂志文章,meta分析

    doi:10.1038/ncomms7065

    authors: van Leeuwen EM,Karssen LC,Deelen J,Isaacs A,Medina-Gomez C,Mbarek H,Kanterakis A,Trompet S,Postmus I,Verweij N,van Enckevort DJ,Huffman JE,White CC,Feitosa MF,Bartz TM,Manichaikul A,Joshi PK,Peloso GM,Deelen P,van D

    更新日期:2015-03-09 00:00:00

  • Phototaxis of synthetic microswimmers in optical landscapes.

    abstract::Many microorganisms, with phytoplankton and zooplankton as prominent examples, display phototactic behaviour, that is, the ability to perform directed motion within a light gradient. Here we experimentally demonstrate that sensing of light gradients can also be achieved in a system of synthetic photo-activated micropa...

    journal_title:Nature communications

    pub_type: 杂志文章

    doi:10.1038/ncomms12828

    authors: Lozano C,Ten Hagen B,Löwen H,Bechinger C

    更新日期:2016-09-30 00:00:00

  • Charge stripe order near the surface of 12-percent doped La₂-xSrxCuO₄.

    abstract::A collective order of spin and charge degrees of freedom into stripes has been predicted to be a possible ground state of hole-doped CuO(2) planes, which are the building blocks of high-temperature superconductors. In fact, stripe-like spin and charge order has been observed in various layered cuprate systems. For the...

    journal_title:Nature communications

    pub_type: 杂志文章

    doi:10.1038/ncomms2019

    authors: Wu HH,Buchholz M,Trabant C,Chang CF,Komarek AC,Heigl F,Zimmermann MV,Cwik M,Nakamura F,Braden M,Schüßler-Langeheine C

    更新日期:2012-01-01 00:00:00

  • Auditory experience-dependent cortical circuit shaping for memory formation in bird song learning.

    abstract::As in human speech acquisition, songbird vocal learning depends on early auditory experience. During development, juvenile songbirds listen to and form auditory memories of adult tutor songs, which they use to shape their own vocalizations in later sensorimotor learning. The higher-level auditory cortex, called the ca...

    journal_title:Nature communications

    pub_type: 杂志文章

    doi:10.1038/ncomms11946

    authors: Yanagihara S,Yazaki-Sugiyama Y

    更新日期:2016-06-21 00:00:00