Abstract:
:TOYOPEARL particles are cross-linked hydroxylated methacrylic polymers available in different pore and particle sizes. They are conjugated with different ligands to generate ion-exchange, hydrophobic interaction and affinity resins. They have excellent physical and chemical properties. A mixed-mode resin, TOYOPEARL MX-Trp-650M, is made of this particle with tryptophan conjugated via N-terminal amino group and hence has both hydrophobic/aromatic side chain and carboxyl group. In this review, I will summarize the properties of the TOYOPEARL particles and MX-Trp-650M resin and application of this resin for purification of proteins and in some detail the separation of disulfide (SS)- scrambled oligomers of insulin-like growth factor-1 (IGF-1). For this particular application, the intact IGF-1 was used to examine binding and elution conditions of TOYOSCREEN MX-Trp-650M column. Strong binding was obtained at pH 4.0, at which arginine, but not NaCl, resulted in elution. Both NaCl and arginine resulted in elution at pH 6.5. In addition, a pH gradient from 4.0 to 8.5 was effective. When applied to SS-scrambled IGF-1 oligomers, both pH and arginine gradient exhibited an efficient separation of the oligomers.
journal_name
Curr Protein Pept Scijournal_title
Current protein & peptide scienceauthors
Arakawa Tdoi
10.2174/1389203718666171009111355subject
Has Abstractpub_date
2019-01-01 00:00:00pages
61-64issue
1eissn
1389-2037issn
1875-5550pii
CPPS-EPUB-86262journal_volume
20pub_type
杂志文章,评审abstract::Natural products serve as a main resource for drug discovery. The ubiquitin-proteasome system (UPS) is one of the primary intracellular protein degradation systems, which is responsible for the degradation of most short-lived, mis-folded and aged proteins. The proteasome is a validated target for cancer treatment, sin...
journal_title:Current protein & peptide science
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journal_title:Current protein & peptide science
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journal_title:Current protein & peptide science
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journal_title:Current protein & peptide science
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journal_title:Current protein & peptide science
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journal_title:Current protein & peptide science
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journal_title:Current protein & peptide science
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journal_title:Current protein & peptide science
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journal_title:Current protein & peptide science
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journal_title:Current protein & peptide science
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