Abstract:
:While the crucial role of intrinsically disordered proteins (IDPs) in the cell cycle is now recognized, deciphering their molecular mode of action at the structural level still remains highly challenging and requires a combination of many biophysical approaches. Among them, small angle X-ray scattering (SAXS) has been extremely successful in the last decade and has become an indispensable technique for addressing many of the fundamental questions regarding the activities of IDPs. After introducing some experimental issues specific to IDPs and in relation to the latest technical developments, this article presents the interest of the theory of polymer physics to evaluate the flexibility of fully disordered proteins. The different strategies to obtain 3-dimensional models of IDPs, free in solution and associated in a complex, are then reviewed. Indeed, recent computational advances have made it possible to readily extract maximum information from the scattering curve with a special emphasis on highly flexible systems, such as multidomain proteins and IDPs. Furthermore, integrated computational approaches now enable the generation of ensembles of conformers to translate the unique flexible characteristics of IDPs by taking into consideration the constraints of more and more various complementary experiment. In particular, a combination of SAXS with high-resolution techniques, such as x-ray crystallography and NMR, allows us to provide reliable models and to gain unique structural insights about the protein over multiple structural scales. The latest neutron scattering experiments also promise new advances in the study of the conformational changes of macromolecules involving more complex systems.
journal_name
Curr Protein Pept Scijournal_title
Current protein & peptide scienceauthors
Receveur-Brechot V,Durand Ddoi
10.2174/138920312799277901subject
Has Abstractpub_date
2012-02-01 00:00:00pages
55-75issue
1eissn
1389-2037issn
1875-5550pii
BSP/CPPS/E-Pub/166journal_volume
13pub_type
杂志文章,评审abstract::The absence of uracil from DNA genomes is a consequence of enzyme functions that eliminate intracellular dUTP pools and that purposefully recognize and remove uracil moieties from DNA. These enzymatic functions are dUTP nucleotidohydrolase (dUTPase) and uracil-DNA glycosylase (UDG), respectively. There are distinct nu...
journal_title:Current protein & peptide science
pub_type: 杂志文章,评审
doi:10.2174/1389203013381044
更新日期:2001-12-01 00:00:00
abstract::The bioactive peptides are protein fragments which have a positive impact on the intestinal homeostasis. Intestinal homeostasis depends on the diverse functions of intestinal barrier including the microbiological, physical, chemical and immunological barriers. Defects in intestinal barrier function are associated with...
journal_title:Current protein & peptide science
pub_type: 杂志文章,评审
doi:10.2174/1389203716666150630134804
更新日期:2015-01-01 00:00:00
abstract::Viscosity of protein solution is one of the most troublesome issues for the high-concentration formulation of protein drugs. In this review, we summarize the practical methods that suppress the viscosity of protein solution using small molecular additives. The small amount of salts decreases the viscosity that results...
journal_title:Current protein & peptide science
pub_type: 杂志文章,评审
doi:10.2174/1389203719666171213114919
更新日期:2018-01-01 00:00:00
abstract::Many naturally occurring bioactive peptides bind to biological membranes. Studying and elucidating the mode of interaction is often an essential step to understand their molecular and biological functions. To obtain the complete orientation and immersion depth of such compounds in the membrane or a membrane-mimetic sy...
journal_title:Current protein & peptide science
pub_type: 杂志文章,评审
doi:10.2174/138920312800785049
更新日期:2012-05-01 00:00:00
abstract::Alpha-fetoprotein (AFP), known largely as a growth-promoting agent, possesses a growth-inhibitory motif recently identified as an occult epitopic segment in the third domain. The present study reviews the multiple biological activities of this AFP-derived peptide segment termed the Growth Inhibitory Peptide (GIP), whi...
journal_title:Current protein & peptide science
pub_type: 杂志文章,评审
doi:10.2174/138920306775474130
更新日期:2006-02-01 00:00:00
abstract::Secondary structure elements often mediate protein-protein interactions. Despite their low abundance in folded proteins, polyproline II (PPII) and its variant, the triple helix, are frequently involved in protein-protein interactions, likely due to their peculiar propensity to be solvent-exposed. We here review the ro...
journal_title:Current protein & peptide science
pub_type: 杂志文章,评审
doi:10.2174/138920312804871157
更新日期:2012-12-01 00:00:00
abstract::The aggregation of proteins or their digested fragments through β-sheet structures has a great significance because it leads to neurodegenerative diseases, which are a problem of the aging societies of the developed countries. Short peptides are typically used as models to study the formation of specific structures. H...
journal_title:Current protein & peptide science
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更新日期:2017-01-01 00:00:00
abstract::The complement system is a major effector arm of the immune defense contributing to the destruction of invading pathogens. There are three possible routes of complement cascade activation: the classical, the alternative and the lectin pathways. The activation of the classical and lectin pathways is initiated by supram...
journal_title:Current protein & peptide science
pub_type: 杂志文章,评审
doi:10.2174/1389203013381242
更新日期:2001-03-01 00:00:00
abstract::The majority of tRNA studies has focused on tRNA molecules as pivotal player in the fundamental process of protein synthesis. Mounting studies have unveiled further functions for tRNA beyond protein synthesis, including non-ribosomal amino acid transfer, and regulation of targeted proteolysis. Post-translational N-ter...
journal_title:Current protein & peptide science
pub_type: 杂志文章,评审
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更新日期:2018-01-01 00:00:00
abstract::The High mobility group box 1 (HMGB1) protein is an extremely versatile, highly conserved nuclear protein, with its unique intracellular and extracellular functions mediated by its relatively simple domain structure. Within the nucleus, HMGB1 binds to DNA minor groove in a nonspecific manner and causes bends in the do...
journal_title:Current protein & peptide science
pub_type: 杂志文章,评审
doi:10.2174/1389203717666160226145217
更新日期:2016-01-01 00:00:00
abstract::Pathogenic aggregation is closely associated with various protein misfolding diseases such as type 2 diabetes mellitus and Alzheimer's disease. Amyloidogenic proteins that have a propensity to assemble into amyloid oligomers and fibrils form the aggregates. The tumor suppressor p53, a transcription factor that regulat...
journal_title:Current protein & peptide science
pub_type: 杂志文章,评审
doi:
更新日期:2015-01-01 00:00:00
abstract::Evidence is accumulating that small open reading frames (sORF, <100 codons) play key roles in many important biological processes. Yet, they are generally ignored in gene annotation despite they are far more abundant than the genes with more than 100 codons. Here, we demonstrate that popular homolog search and codon-i...
journal_title:Current protein & peptide science
pub_type: 杂志文章,评审
doi:10.2174/138920311796957667
更新日期:2011-09-01 00:00:00
abstract::The cytolysin is a novel, two-peptide lytic toxin produced by some strains of Enterococcus faecalis. It is toxic in animal models of enterococcal infection, and associated with acutely terminal outcome in human infection. The cytolysin exerts activity against a broad spectrum of cell types including a wide range of gr...
journal_title:Current protein & peptide science
pub_type: 杂志文章,评审
doi:10.2174/1389203053027557
更新日期:2005-02-01 00:00:00
abstract::Proteasomes are the major nonlysosomal protein degradation machinery in eukaryotic cells and they are largely responsible for the processing of antigens for presentation by the MHC class I pathway. This review concentrates on recent developments in the area of antigen processing. Specialized proteasomes called immunop...
journal_title:Current protein & peptide science
pub_type: 杂志文章,评审
doi:10.2174/1389203043379774
更新日期:2004-06-01 00:00:00
abstract::ATPases are important molecular machines that convert the chemical energies stored in ATP to mechanical actions within the cell. ATPases are among the most abundant proteins with diverse functions involved in almost every cellular pathway. The well characterised ATPases include the various motor proteins responsible f...
journal_title:Current protein & peptide science
pub_type: 杂志文章
doi:10.2174/1389203043486874
更新日期:2004-04-01 00:00:00
abstract::Calmodulin (CaM) is a highly conserved eukaryotic Ca2+ sensor protein that is able to bind a large variety of target sequences without a defined consensus sequence. The recognition of this diverse target set allows CaM to take part in the regulation of several vital cell functions. To fully understand the structural b...
journal_title:Current protein & peptide science
pub_type: 杂志文章,评审
doi:10.2174/1389203720666190925101937
更新日期:2019-01-01 00:00:00
abstract::Elevated blood pressure or hypertension is one of the fastest growing health problems worldwide. Although the etiology of essential hypertension has a genetic component, dietary factors play an important role. With the high costs and adverse side-effects associated with synthetic antihypertensive drugs and the awarene...
journal_title:Current protein & peptide science
pub_type: 杂志文章,评审
doi:10.2174/13892037113149990036
更新日期:2013-05-01 00:00:00
abstract::Knowledge of the existence and structure of intermediates on the reaction pathway is necessary before specific details of the mechanism may be successfully resolved. However, enzymatic catalysis is an extremely fast process. This rapidity of enzyme-catalyzed reactions and the short life times of intermediates represen...
journal_title:Current protein & peptide science
pub_type: 杂志文章,评审
doi:10.2174/138920309789351958
更新日期:2009-10-01 00:00:00
abstract::Fungal diseases are severe and have very high morbidity as well as up to 60% mortality for patients diagnosed with invasive fungal infection. In this review, in vitro and in vivo studies provided us with the insight into the role of Candida virulence factors that mediate their success as pathogens, such as: membrane a...
journal_title:Current protein & peptide science
pub_type: 杂志文章,评审
doi:10.2174/1389203720666190722152415
更新日期:2020-01-01 00:00:00
abstract::MEP (mercapto-ethyl-pyridine) HyperCel is one of the hydrophobic charge induction chromatography (HCIC) resins. Under normal operation, proteins are bound to the MEP resin at neutral pH, at which MEP is not charged, mostly via hydrophobic interaction. MEP has a pyridine group, whose pK is 4.8, and hence is positively ...
journal_title:Current protein & peptide science
pub_type: 杂志文章,评审
doi:10.2174/1389203718666171117105132
更新日期:2019-01-01 00:00:00
abstract::Protein-protein interaction (PPI) is necessary for most of the biological processes and requisite for host-pathogen communication. Most of the threatening human diseases are caused by different types of interactions of proteins with their prior infected proteins or with pathogen's proteins. Understanding of involved m...
journal_title:Current protein & peptide science
pub_type: 杂志文章,评审
doi:10.2174/1389203718666170828122927
更新日期:2018-01-01 00:00:00
abstract::Peptides act as promising anticancer agents due to their ease of synthesis and modifications, enhanced tumor penetration, and less systemic toxicity. However, only limited success has been achieved so far, as experimental design and synthesis of anticancer peptides (ACPs) are prohibitively costly and time-consuming. F...
journal_title:Current protein & peptide science
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更新日期:2020-01-01 00:00:00
abstract::Lactation is a critical phase for brain function development. New dietary experiences of mouse caused by weaning can regulate brain development and function, increase their response to food and environment, and eventually give rise to corresponding behavioral changes. Changes in weaning time induce the alteration of b...
journal_title:Current protein & peptide science
pub_type: 杂志文章,评审
doi:10.2174/1389203720666190125095819
更新日期:2019-01-01 00:00:00
abstract::Covalent conjugation of the ubiquitin tag to cellular proteins plays a central role in a number of processes, the most notable among them being degradation by the 26S proteasome. A fundamental property of this process is that ubiquitination, in contrast to subsequent degradation, is reversible due to a number of deubi...
journal_title:Current protein & peptide science
pub_type: 杂志文章,评审
doi:10.2174/1389203043379756
更新日期:2004-06-01 00:00:00
abstract::Cardiovascular diseases (CVDs) are the leading cause of mortality worldwide. Rehmanniae Radix Praeparata (RRP) is a popular medicinal herb widely used in traditional Chinese medicine (TCM) to treat CVDs. However, the development of this novel therapeutic product has been stagnant, and its molecular mechanism of action...
journal_title:Current protein & peptide science
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更新日期:2019-01-01 00:00:00
abstract::There has been a tremendous increase in the number of approved drugs derived from recombinant proteins; however, their development as potential drugs has been hampered by their instability that causes difficulty to formulate them as therapeutic agents. It has been shown that the reactivity of thiol and disulfide funct...
journal_title:Current protein & peptide science
pub_type: 杂志文章,评审
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更新日期:2009-12-01 00:00:00
abstract::Incidence of fungal infections has increased alarmingly in past few decades. Of the fungal pathogens, the Aspergillus fumigatus has been a major cause of allergic bronchopulmonary aspergillosis (ABPA) which has five main stages--the acute, remission, exacerbation, glucocorticoid dependent and fibrotic stage. The diagn...
journal_title:Current protein & peptide science
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更新日期:2014-01-01 00:00:00
abstract::Intestines are not only major organs for nutrient digestion and absorption, but are also the largest immune organ in pigs. They are essential for maintaining the health and growth of piglets. Fatty acids, including short-chain fatty acids, medium-chain fatty acids, and long-chain polyunsaturated fatty acids, are impor...
journal_title:Current protein & peptide science
pub_type: 杂志文章,评审
doi:10.2174/1389203720666190514120023
更新日期:2019-01-01 00:00:00
abstract::Fbxo7/PARK15 has well-defined roles, acting as part of a Skp1-Cul1-F box protein (SCF)- type E3 ubiquitin ligase and also having SCF-independent activities. Mutations within FBXO7 have been found to cause an early-onset Parkinson's disease, and these are found within or near to its functional domains, including its F-...
journal_title:Current protein & peptide science
pub_type: 杂志文章,评审
doi:10.2174/1389203717666160311121433
更新日期:2017-01-01 00:00:00
abstract::The Mastermind-like (MAML) proteins are transcriptional coactivators for Notch signaling, an evolutionarily conserved pathway that plays several key roles in development and in human disease. The MAML family contains MAML1, MAML2, and MAML3. More recently, MAML1 has been shown to function as a coactivator for the tumo...
journal_title:Current protein & peptide science
pub_type: 杂志文章,评审
doi:10.2174/138920309789630543
更新日期:2009-12-01 00:00:00