Abstract:
:Evidence is accumulating that small open reading frames (sORF, <100 codons) play key roles in many important biological processes. Yet, they are generally ignored in gene annotation despite they are far more abundant than the genes with more than 100 codons. Here, we demonstrate that popular homolog search and codon-index techniques perform poorly for small genes relative to that for larger genes, while a method dedicated to sORF discovery has a similar level of accuracy as homology search. The result is largely due to the small dataset of experimentally verified sORF available for homology search and for training ab initio techniques. It highlights the urgent need for both experimental and computational studies in order to further advance the accuracy of sORF prediction.
journal_name
Curr Protein Pept Scijournal_title
Current protein & peptide scienceauthors
Cheng H,Chan WS,Li Z,Wang D,Liu S,Zhou Ydoi
10.2174/138920311796957667subject
Has Abstractpub_date
2011-09-01 00:00:00pages
503-7issue
6eissn
1389-2037issn
1875-5550pii
CPPS-143journal_volume
12pub_type
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