Abstract:
:MEP (mercapto-ethyl-pyridine) HyperCel is one of the hydrophobic charge induction chromatography (HCIC) resins. Under normal operation, proteins are bound to the MEP resin at neutral pH, at which MEP is not charged, mostly via hydrophobic interaction. MEP has a pyridine group, whose pK is 4.8, and hence is positively charged at acidic pH range. Based on the binding mechanism (i.e., hydrophobic interaction) and the induced positive charge at acidic pH, there may be two ways to elute the bound proteins. One way is to bring the pH down to protonate both MEP resin and the bound protein, leading to charge repulsion and thereby elution. Another way is to use hydrophobic interaction modifiers, which are often used in hydrophobic interaction chromatography, to reduce hydrophobic interaction. Here, we summarize such two possible elution approaches.
journal_name
Curr Protein Pept Scijournal_title
Current protein & peptide scienceauthors
Arakawa T,Tokunaga M,Maruyama T,Shiraki Kdoi
10.2174/1389203718666171117105132subject
Has Abstractpub_date
2019-01-01 00:00:00pages
28-33issue
1eissn
1389-2037issn
1875-5550pii
CPPS-EPUB-86935journal_volume
20pub_type
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