Holistic protocol for callus culture optimization using statistical modelling.

Abstract:

:Plants endue a key role against illnesses caused by oxidative stress. These attributes are frequently associated with polyphenolic compounds. However, presence and concentration of secondary metabolites are affected by abiotic factors. The in vitro culture techniques can solve these drawbacks. Peppers can be a suitable alternative to obtain polyphenols. Aiming to optimise the callus culture stage from Capsicum baccatum to produce polyphenols, this work evaluated systemically the effects of the explant's origin (root, hypocotyl and cotyledon), growth hormone type (2,4-dichlorophenoxyacetic acid (2,4-D), benzylaminopurine (BAP) and a combination of 2,4-D/BAP at five-to-one ratio) and concentration (0.023-10.000 mg L-1) on callus culture efficiency parameters using a multilevel factorial design. The root explant in combination with BAP at 1.138 mg L-1 ensured the optimal values of the assessed responses; ​callus mass (225.03 mg), antioxidant activity (35.95%), total phenols (11.48 mg of GAE/g DE) and flavonoids (15.92 mg of RU/g DE) production.

journal_name

Nat Prod Res

journal_title

Natural product research

authors

Fidemann T,de Araujo Pereira GA,Bossard Nascimento L,Moraes MC,Bertão MR,da Silva RMG,Fernández Núñez EG

doi

10.1080/14786419.2017.1380026

subject

Has Abstract

pub_date

2018-05-01 00:00:00

pages

1109-1117

issue

9

eissn

1478-6419

issn

1478-6427

journal_volume

32

pub_type

杂志文章
  • A validated HPTLC method for determination of trans-caryophyllene from polyherbal formulations.

    abstract::Formulations of traditional medicines are usually made up of a complex mixture of herbs. However, effective quality control methods in order to select materials of the right quality are lacking. 'Amukkara choornam' is a polyherbal Siddha formulation used for gastritis, spleen enlargement, leucorrhoea, hiccups, anaemia...

    journal_title:Natural product research

    pub_type: 杂志文章

    doi:10.1080/14786419.2010.497147

    authors: Patra KC,Singh B,Pareta S,Kumar KJ

    更新日期:2010-12-01 00:00:00

  • A new coumarin from the fruits of Coutarea hexandra.

    abstract::A new 5-O-beta-D-glucopyranosyl-4-(4-hydroxyphenyl)-7-methoxy-2H-chromen-2-one (1), together with four known compounds, one coumarin, 5-O-beta-D-galactopyranosyl-4-(4-hydroxyphenyl)-7-methoxy-2H-chromen-2-one (2) and three cucurbitacins, 23,24-dihydrocucurbitacin F (3), 23,24-dihydro-25-acetylcucurbitacin F (4) and 2-...

    journal_title:Natural product research

    pub_type: 杂志文章

    doi:10.1080/14786410701371116

    authors: Olmedo D,Rodríguez N,Vásquez Y,Solís PN,López-Pérez JL,Feliciano AS,Gupta MP

    更新日期:2007-06-01 00:00:00

  • A new isocoumarin from the aerial parts of Aconitum gymnandrum.

    abstract::A new isocoumarin, along with 10 known compounds, was isolated from the aerial parts of Aconitum gymnandrum. Their structures were elucidated by spectroscopic methods including extensive 1D and 2D NMR techniques. Among the known compounds, compound 11 was obtained as a natural product for the first time, which was pre...

    journal_title:Natural product research

    pub_type: 杂志文章

    doi:10.1080/14786419.2015.1137574

    authors: Zhao JQ,Wang YM,Wang S,Dang J,Shi YP,Mei LJ,Tao YD

    更新日期:2016-08-01 00:00:00

  • Styrylpyrone, isolated from an Amazon plant, induces cell cycle arrest and autophagy in Leishmania amazonensis.

    abstract::The search for bioactive compounds against diseases is imperative and the richness of the Amazon provides a large source to be explored. Current therapies for the treatment of parasitic infections have severe side effects and low efficacy, which makes the development of an effective chemotherapy extremely important. I...

    journal_title:Natural product research

    pub_type: 杂志文章

    doi:10.1080/14786419.2020.1715395

    authors: Fernandes NS,Desoti VC,Dias A,da Silva YC,de Azevedo Dos Santos AP,Passarini GM,Nakamura CV,da Veiga Junior VF

    更新日期:2020-01-25 00:00:00

  • A new ent-kaurane diterpene from Euphorbia stracheyi Boiss.

    abstract::The chemical investigation of whole plants Euphorbia stracheyi Boiss from China led to the isolation of one new ent-kaurane diterpene, ent-Kaurane-16β,17,19-triol-3-one (1), along with three known ent-kaurane diterpenoids (2-4) as ent-Kaurane-3α,16β,17-triol (2),ent-16S,17-dihydroxy-kaurane-3-one (3) and ent-3S,16S,17...

    journal_title:Natural product research

    pub_type: 杂志文章

    doi:10.1080/14786419.2016.1222385

    authors: Liu T,Liang Q,Xiong NN,Dai LF,Wang JM,Ji XH,Xu WH

    更新日期:2017-01-01 00:00:00

  • Topical anti-inflammatory constituents of lipophilic leaf fractions of Alchornea floribunda and Alchornea cordifolia.

    abstract::The leaves of Alchornea floribunda and Alchornea cordifolia are used traditionally as topical anti-inflammatory agents. In this study, two highly lipophilic fractions AFLF and ACLF isolated from A. floribunda and A. cordifolia leaves respectively were investigated for topical anti-inflammatory effects using xylene-ind...

    journal_title:Natural product research

    pub_type: 杂志文章

    doi:10.1080/14786419.2010.512272

    authors: Okoye FB,Osadebe PO,Nworu CS,Okoye NN,Omeje EO,Esimone CO

    更新日期:2011-12-01 00:00:00

  • Development and validation of headspace Solid-Phase microextraction coupled with gas chromatography (HS-SPME-GC) method for the analysis of zingiber zerumbet L.

    abstract::Headspace solid-phase microextraction (HS-SPME) coupled with gas chromatography (GC-FID) was explored to determine the fingerprinting characteristic of Zingiber zerumbet L. volatiles to differentiate between different ginger species. The effect of different fibers [polydimethylsiloxane (PDMS, 7 μm), polyacrylate (PA, ...

    journal_title:Natural product research

    pub_type: 杂志文章

    doi:10.1080/14786419.2019.1643863

    authors: Bhavya ML,Ravi R,Madhava Naidu M

    更新日期:2019-07-22 00:00:00

  • New steroidal alkaloids from Sarcococca saligna.

    abstract::Two new steroidal alkaloids, salonine-A [(20S)-20-(N,N-dimethylamino)-3beta-(tigloylamino)-5alpha-pregn-14-en-2beta,4beta-diol] (1), and salonine-B [(20S)-20-(N,N-dimethylamino)-3beta-methoxy-pregn-5,16-diene] (2), were isolated from the MeOH extract of Sarcococca saligna, along with a known base, alkaloid-C (3). Thei...

    journal_title:Natural product research

    pub_type: 杂志文章

    doi:10.1080/1057563021000051086

    authors: Atta-ur-Rahman,Feroz F,Zaheer-ul-Haq,Nawaz SA,Khan MR,Choudhary MI

    更新日期:2003-08-01 00:00:00

  • Anti-infective potential of caffeic acid and epicatechin 3-gallate isolated from methanol extract of Euphorbia hirta (L.) against Pseudomonas aeruginosa.

    abstract::Euphorbia hirta (L.) plant is traditionally used in Malaysia for the treatment of gastrointestinal, bronchial and respiratory ailments caused by nosocomial infectious agents. Bioactivity-guided fractionation of the methanol extract of the aerial parts of E. hirta and analysis using high-performance liquid chromatograp...

    journal_title:Natural product research

    pub_type: 杂志文章

    doi:10.1080/14786419.2014.999242

    authors: Perumal S,Mahmud R,Ramanathan S

    更新日期:2015-01-01 00:00:00

  • Optimization of polysaccharides extraction from quince peels: partial characterization, antioxidant and antiproliferative properties.

    abstract::In this study, Box-Behnken Design was used to optimize the ultrasonic extraction of polysaccharides from quince peels (QPPs) by ascorbic acid and the effect of extraction temperature, extraction time and pH was evaluated. Under optimized conditions of temperature 90 °C, 60 min sonication time and pH = 3.26, the extrac...

    journal_title:Natural product research

    pub_type: 杂志文章

    doi:10.1080/14786419.2018.1514403

    authors: Dammak MI,Mzoughi Z,Chakroun I,Mansour HB,Le Cerf D,Majdoub H

    更新日期:2020-05-01 00:00:00

  • Comparative analysis of chemical constituents and bioactivities of the extracts from leaves, seed coats and embryoids of Ginkgo biloba L.

    abstract::A total of 25 compounds including terpenoids, flavonoids, biflavonoids and ginkgolic acids were identified and quantified with a reliable, simple, and simultaneous method from Ginkgo leaves, seed coats and embryoids with different tree ages (approximately identified as 25, 500, 1000 and 2000 years). Leaves had the hig...

    journal_title:Natural product research

    pub_type: 杂志文章

    doi:10.1080/14786419.2020.1788020

    authors: Chen X,Zhong W,Shu C,Yang H,Li E

    更新日期:2020-07-01 00:00:00

  • Chemotaxonomy and cytotoxicity of the liverwort Porella Viridissima.

    abstract::The first chemotaxonomic study based on volatile components of Porella viridissima (Mitt.) Grolle is reported. The GC-MS analysis of ether extract was performed; ten santalane and five pinguisane-type sesquiterpenes were identified together with perrottetianal A as major diterpene. Most of detected santalane-type sesq...

    journal_title:Natural product research

    pub_type: 杂志文章

    doi:10.1080/14786419.2019.1655022

    authors: Métoyer B,Lebouvier N,Hnawia E,Thouvenot L,Wang F,Harinantenaina Rakotondraibe L,Raharivelomanana P,Asakawa Y,Nour M

    更新日期:2019-08-23 00:00:00

  • Production of bioactive tryptamine derivatives by co-culture of marine Streptomyces with Bacillus mycoides.

    abstract::Tryptamine derivatives such as tryptamine and bacillamides were strong algicidal compounds promising in controlling harmful algae blooms, but their bioactivity and application researches were hindered by extremely low natural production rates. This study found an induced production of algicidal tryptamine derivatives ...

    journal_title:Natural product research

    pub_type: 杂志文章

    doi:10.1080/14786419.2015.1005619

    authors: Yu L,Hu Z,Ma Z

    更新日期:2015-01-01 00:00:00

  • Evaluation of the antiviral and antimicrobial activities of triterpenes isolated from Euphorbia segetalis.

    abstract::A phytochemical reinvestigation of the whole plant of Euphorbia segetalis yielded five tetracyclic triterpenes: 3beta-hydroxy-cycloart-25-en-24-one (1), cycloart-25-ene-3beta,24-diol (2), cycloart-23-ene-3beta,25-diol (3), lanosta-7,9(11),24-trien-3beta-ol (4) and lanosta-7,9(11),24(31)-trien-3beta-ol (5). beta-acetox...

    journal_title:Natural product research

    pub_type: 杂志文章

    doi:10.1080/14786410310001605841

    authors: Madureira AM,Ascenso JR,Valdeira L,Duarte A,Frade JP,Freitas G,Ferreira MJ

    更新日期:2003-10-01 00:00:00

  • A new lanostane-type triterpene and sesquiterpene lactones from Vernonia leopoldii and their in vitro cytotoxicity.

    abstract::Investigation of the aerial parts of Vernonia leopoldii (Sch. Bip.) Vatke afforded a new lanostane-type triterpene along with known hirsutinolide-type sesquiterpene lactones and flavonoid glycosides, all are identified for the first time in this species. The new compound was identified as lanost-3β, 23S-dihydroxy-22(3...

    journal_title:Natural product research

    pub_type: 杂志文章

    doi:10.1080/14786419.2015.1062004

    authors: Marzouk AM,Abd Elhalim OB

    更新日期:2016-01-01 00:00:00

  • Separation and purification of ginsenoside Re from ginseng bud by selective adsorption of active carbon and preparative high-performance liquid chromatography.

    abstract::Ginsenoside Re (G-Re) is a major ingredient of the ginseng bud. A novel and rapid method to isolate and purify G-Re from ginseng buds was established. The procedure involves solvent extraction of ginseng bud powder, then pre-purification with an active carbon column and purification by preparative HPLC (prep-HPLC). Ac...

    journal_title:Natural product research

    pub_type: 杂志文章

    doi:10.1080/14786419.2010.540577

    authors: Kuang P,Wang G,Yuan Q,Liang H

    更新日期:2012-01-01 00:00:00

  • Screening of medicinal plants traditionally used in Peruvian Amazon for in vitro antioxidant and anticancer potential.

    abstract::Plants mentioned in this study have numerous records in traditional Peruvian medicine being used in treatment of cancer and other diseases likely to be associated with oxidative stress. Amongst the eight plant species tested, only Dysphania ambrosioides exhibited combinatory antioxidant and anti-proliferative effect o...

    journal_title:Natural product research

    pub_type: 杂志文章

    doi:10.1080/14786419.2018.1462180

    authors: Tauchen J,Huml L,Bortl L,Doskocil I,Jarosova V,Marsik P,Frankova A,Clavo Peralta ZM,Chuspe Zans ME,Havlik J,Lapcik O,Kokoska L

    更新日期:2019-09-01 00:00:00

  • Evidence of cyanamide production in hairy vetch Vicia villosa.

    abstract::Cyanamide (NH(2)CN) has recently been isolated as a plant growth inhibitor from Vicia villosa, which is the first discovery of cyanamide from natural sources. To reveal the presence of the biosynthesized cyanamide in plants, 3.4 mM potassium ((15)N)nitrate was administered to 15- to 35-day-old plants of V. villosa, fr...

    journal_title:Natural product research

    pub_type: 杂志文章

    doi:10.1080/14786410500143583

    authors: Kamo T,Kato K,Hiradate S,Nakajima E,Fujii Y,Hirota M

    更新日期:2006-05-10 00:00:00

  • Evaluation of the volatile profile of Tuber liyuanum by HS-SPME with GC-MS.

    abstract::The volatile components of Tuber liyuanum were determined by HS-SPME with GC-MS for the first time. The effects of different fibre coating, extraction time, extraction temperature and sample amount were studied to get optimal extraction conditions. The optimal conditions were SPME fibre of Carboxen/PDMS, extraction ti...

    journal_title:Natural product research

    pub_type: 杂志文章

    doi:10.1080/14786419.2016.1253082

    authors: Liu C,Li Y

    更新日期:2017-04-01 00:00:00

  • Cytotoxicity of fagaramide derivative and canthin-6-one from Zanthoxylum (Rutaceae) species against multidrug resistant leukemia cells.

    abstract::In our continuous search for cytotoxic compounds from the genus Zanthoxylum, chromatographic separation of the MeOH/CH2Cl2 (1:1) extract of Z. chalybeum yielded one new alkamide; 4-(isoprenyloxy)-3-methoxy-3,4-deoxymethylenedioxyfagaramide (1) and a known one; fagaramide (2). Similarly, from the MeOH/CH2Cl2 (1:1) extr...

    journal_title:Natural product research

    pub_type: 杂志文章

    doi:10.1080/14786419.2019.1587424

    authors: Omosa LK,Mbogo GM,Korir E,Omole R,Seo EJ,Yenesew A,Heydenreich M,Midiwo JO,Efferth T

    更新日期:2019-03-21 00:00:00

  • Isolation and structure elucidation of a new flavonol glycoside from Sabia Parviflora.

    abstract::A new flavonol glycoside named Sabiapside A (1), along with four known compounds, quercetin-3-O-gentiobioside (2), camellianoside (3), isobariclisin-3-O- rutinoside (4), tsubakioside A (5), was isolated from Sabia parviflora. Their structures were elucidated by extensive spectroscopic analysis including MS, UV, IR and...

    journal_title:Natural product research

    pub_type: 杂志文章

    doi:10.1080/14786419.2019.1679130

    authors: Sun Q,Pan G,Xu W,Lu X,Bai C,Liu M,Chen Y

    更新日期:2019-10-29 00:00:00

  • In vitro and in vivo studies of Cucurbita pepo L. flowers: chemical profile and bioactivity.

    abstract::Edible flowers consumption has increased in recent years due to their rich content of healthy phytochemicals. The aim of this study was to analyse the chemical profile of Cucurbita pepo L. flowers, and to explore their antioxidant and hypoglycaemic properties. Moreover, in order to assess in vivo effects, biochemical ...

    journal_title:Natural product research

    pub_type: 杂志文章

    doi:10.1080/14786419.2019.1672067

    authors: Morittu VM,Musco N,Mastellone V,Bonesi M,Britti D,Infascelli F,Loizzo MR,Tundis R,Sicari V,Tudisco R,Lombardi P

    更新日期:2019-09-30 00:00:00

  • A new eremophilenolactone from Senecio nemorensis.

    abstract::A new eremophilane sesquiterpene was isolated from the roots of Senecio nemorensis. Its structure was established as 10alpha-hydroxy-6beta-propionyloxy-1-oxoeremophila-7(11),8-dieno-12,8-lactone on the basis of spectral analysis. ...

    journal_title:Natural product research

    pub_type: 杂志文章

    doi:10.1080/14786410801927940

    authors: Meng FJ,Xiao YN,Zhao RJ,Zhao H,Ma QL,Xie WD

    更新日期:2009-01-01 00:00:00

  • Amphibian skin extracts as source of bioactive multi-target agents against different pathways of Alzheimer's disease.

    abstract::Natural products represent a rich source of bioactive compounds that have been historically used to obtain substances with great medicinal potential. The skin of anuran amphibians is a rich source of compounds with a wide range of biological activity. Alzheimer's disease (AD) is a complex disease associated with all k...

    journal_title:Natural product research

    pub_type: 杂志文章

    doi:10.1080/14786419.2019.1591396

    authors: Spinelli R,Aimaretti FM,López JA,Siano AS

    更新日期:2019-03-31 00:00:00

  • Bioactive sesquiterpenoids and steroids from the resinous exudates of Commiphora myrrha.

    abstract::Chemical investigation of the resinous exudates of Commiphora myrrha has led to the isolation of four sesquiterpenes (1a/1b, 2, and 3), including one pair of new sesquiterpene enantiomers (1a/1b), one new racemic mixture 2, and two steroids (4 and 5). Their structures were elucidated by spectroscopic analysis, and the...

    journal_title:Natural product research

    pub_type: 杂志文章

    doi:10.1080/14786419.2018.1448811

    authors: Ge CY,Zhang JL

    更新日期:2019-02-01 00:00:00

  • A new monoterpenoid indole alkaloid from Ochrosia elliptica.

    abstract::A new monoterpenoid indole alkaloid, 10-methoxyakuammidine (1), together with four known alkaloids (2-5), were isolated from the stems and leaves of Ochrosia elliptica. The structure of 1 was elucidated by extensive spectroscopic methods and the known compounds were identified by comparisons with data reported in the ...

    journal_title:Natural product research

    pub_type: 杂志文章

    doi:10.1080/14786419.2016.1277349

    authors: Chen AH,Liu QL,Ma YL,Jiang ZH,Tang JY,Liu YP,Chen GY,Fu YH,Xu W

    更新日期:2017-07-01 00:00:00

  • Natural male contraceptive: phytochemical investigation and anti-spermatogenic activity of Pistia stratiotes Linn.

    abstract::This work is an attempt to explore the anti-spermatogenic activity of Pistia stratiotes and to investigate it as a male contraceptive. The prepared extracts were screened for the presence of alkaloids, glycosides, steroids, flavonoids, saponin and phenolic compounds. To assess the anti-spermatogenic activity, mice wer...

    journal_title:Natural product research

    pub_type: 杂志文章

    doi:10.1080/14786419.2014.900772

    authors: Singh K,Dubey BK,Tripathi AC,Singh AP,Saraf SK

    更新日期:2014-01-01 00:00:00

  • Antioxidant, nitric oxide scavenging and malondialdehyde scavenging activities of essential oils from different chemotypes of Zataria multiflora.

    abstract::The antioxidant, nitric oxide (NO) scavenging and malondialdehyde (MDA) scavenging activities of different Zataria multiflora (ZM) chemotype essential oils (EOs) were investigated. The main components are: ZM1 (carvacrol, p-cymene), ZM2 (carvacrol, p-cymene), ZM3 (carvacrol, p-cymene), ZM4 (linalool), ZM5 (carvacrol, ...

    journal_title:Natural product research

    pub_type: 杂志文章

    doi:10.1080/14786419.2011.631136

    authors: Karimian P,Kavoosi G,Saharkhiz MJ

    更新日期:2012-01-01 00:00:00

  • Enantiomeric synthesis of natural alkylglycerols and their antibacterial and antibiofilm activities.

    abstract::Alkylglycerols (AKGs) are bioactive natural compounds that vary by alkyl chain length and degree of unsaturation, and their absolute configuration is 2S. Three AKGs (5l-5n) were synthesised in enantiomerically pure form, and were characterised for the first time together with 12 other known and naturally occurring AKG...

    journal_title:Natural product research

    pub_type: 杂志文章

    doi:10.1080/14786419.2019.1686370

    authors: Fernández Montoya DJ,Contreras Jordan LA,Moreno-Murillo B,Silva-Gómez E,Mayorga-Wandurraga H

    更新日期:2019-11-04 00:00:00

  • Antibacterial effects of Brazilian and Bulgarian propolis and synergistic effects with antibiotics acting on the bacterial DNA and folic acid.

    abstract::Propolis is a honeybee product that has been used since ancient times because of its therapeutic effects. It can be used in the development of alternative therapies for the treatment of many diseases, and because propolis shows antibacterial action, this work was carried out in order to investigate a possible synergis...

    journal_title:Natural product research

    pub_type: 杂志文章

    doi:10.1080/14786411003754355

    authors: Orsi RO,Fernandes A Jr,Bankova V,Sforcin JM

    更新日期:2012-01-01 00:00:00