A validated HPTLC method for determination of trans-caryophyllene from polyherbal formulations.

Abstract:

:Formulations of traditional medicines are usually made up of a complex mixture of herbs. However, effective quality control methods in order to select materials of the right quality are lacking. 'Amukkara choornam' is a polyherbal Siddha formulation used for gastritis, spleen enlargement, leucorrhoea, hiccups, anaemia, tuberculosis and kappa diseases. Trans-caryophyllene is an important constituent present in the ingredients of this formulation. In a literature survey, it was found that there is no such method for the quantification of trans-caryophyllene except gas chromatography or gas chromatography-mass spectroscopy (GC-MS). So, a high performance thin layer chromatography (HPTLC) method was developed and validated for the quantification of trans-caryophyllene in amukkara choornam. Pre-coated silica gel 60F-254 plates (10 × 10 cm²) were used for the analysis. The solvent system consisted of toluene-ethyl acetatate (9 : 3, v/v), and trans-caryophyllene was detected at 260 nm. The developed method was validated for linearity (R² = 0.9996 ± 0.0034), limit of detection (LOD) (0.101 ng), limit of quantification (LOQ) (0.639 ng), accuracy (% recovery = 97.19 ± 1.204), and precision (CV < 5%, for both intra-day and inter-day precisions). The levels of trans-caryophyllene were found to be 3.5-4.10 µg per gram of herbal products.

journal_name

Nat Prod Res

journal_title

Natural product research

authors

Patra KC,Singh B,Pareta S,Kumar KJ

doi

10.1080/14786419.2010.497147

subject

Has Abstract

pub_date

2010-12-01 00:00:00

pages

1933-8

issue

20

eissn

1478-6419

issn

1478-6427

pii

930025126

journal_volume

24

pub_type

杂志文章
  • Plants of the Russian Federation pharmacopeia: an unexhausted natural products research opportunity?

    abstract::32 medicinal plants that are unique to the Russian Pharmacopeia have 693 isolated natural products associated with them (an average of 21.66 compounds per plant). This is in contrast to the US Pharmacopeia plants that have delivered 2366 known natural products (an average of 42.25 compounds per plant). ...

    journal_title:Natural product research

    pub_type: 信件

    doi:10.1080/14786419.2020.1727474

    authors: Chupakhin E,Babich O,Prosekov A,Asyakina L,Gureev M,Krasavin M

    更新日期:2020-02-21 00:00:00

  • New alkaloids from the leaves of Evodia rutaecarpa.

    abstract::One new indole alkaloid (1) and one new indole alkaloidal glycoside (2), together with nine known alkaloids (3-11), were isolated from the leaves of Evodia rutaecarpa. Their structures were determined on the basis of spectroscopic and chemical methods. Compound 4 exhibited potent activity against Pseudomonas aeruginos...

    journal_title:Natural product research

    pub_type: 杂志文章

    doi:10.1080/14786419.2016.1146888

    authors: Xia X,Luo JG,Liu RH,Yang MH,Kong LY

    更新日期:2016-10-01 00:00:00

  • Paraherquamide J, a new prenylated indole alkaloid from the marine-derived fungus Penicillium janthinellum HK1-6.

    abstract::A new prenylated indole alkaloid, named paraherquamide J (1), together with four known compounds (2-5), were isolated from the mangrove rhizosphere soil-derived fungus Penicillium janthinellum HK1-6. The planar structure and relative configuration of 1 were determined by detailed analysis of the spectroscopic data esp...

    journal_title:Natural product research

    pub_type: 杂志文章

    doi:10.1080/14786419.2018.1534105

    authors: Zheng YY,Shen NX,Liang ZY,Shen L,Chen M,Wang CY

    更新日期:2020-02-01 00:00:00

  • Identification of a cDNA clone specific for the taxol synthesis phase of Taxus chinensis cells by mRNA differential display.

    abstract::In order to identify genes related to Taxol biosynthesis, the mRNA differential display method was employed to compare mRNA populations from suspension cultured Taxus chinensis cells before and after beginning to produce Taxol. From a total display of about 4000 PCR products, 104 were derived from cells during the Tax...

    journal_title:Natural product research

    pub_type: 杂志文章

    doi:10.1080/14786410310001630573

    authors: Hu G,Fan T,Mei X

    更新日期:2004-08-01 00:00:00

  • Topical anti-inflammatory constituents of lipophilic leaf fractions of Alchornea floribunda and Alchornea cordifolia.

    abstract::The leaves of Alchornea floribunda and Alchornea cordifolia are used traditionally as topical anti-inflammatory agents. In this study, two highly lipophilic fractions AFLF and ACLF isolated from A. floribunda and A. cordifolia leaves respectively were investigated for topical anti-inflammatory effects using xylene-ind...

    journal_title:Natural product research

    pub_type: 杂志文章

    doi:10.1080/14786419.2010.512272

    authors: Okoye FB,Osadebe PO,Nworu CS,Okoye NN,Omeje EO,Esimone CO

    更新日期:2011-12-01 00:00:00

  • Xanthones from Garcinia mangostana (Guttiferae).

    abstract::Studies on the stem of Garcinia mangostana have led to the isolation of one new xanthone mangosharin (1) (2,6-dihydroxy-8-methoxy-5-(3-methylbut-2-enyl)-xanthone) and six other prenylated xanthones, alpha-mangostin (2), beta-mangostin (3), garcinone D (4), 1,6-dihydroxy-3,7-dimethoxy-2-(3-methylbut-2-enyl)-xanthone (5...

    journal_title:Natural product research

    pub_type: 杂志文章

    doi:10.1080/14786410500463114

    authors: Ee GC,Daud S,Taufiq-Yap YH,Ismail NH,Rahmani M

    更新日期:2006-10-01 00:00:00

  • Medicinal plants with anti-inflammatory activities.

    abstract::Medicinal plants have been the main remedy to treat various ailments for a long time and nowadays, many drugs have been developed from traditional medicine. This paper reviews some medicinal plants and their main constituents which possess anti-inflammatory activities useful for curing joint inflammation, inflammatory...

    journal_title:Natural product research

    pub_type: 杂志文章,评审

    doi:10.1080/14786419.2015.1062761

    authors: Maione F,Russo R,Khan H,Mascolo N

    更新日期:2016-06-01 00:00:00

  • Antioxidant properties and principal phenolic phytochemicals of Indian medicinal plants from Asclepiadoideae and Periplocoideae.

    abstract::The subfamily Asclepiadoideae (Apocynaceae) and the closely-related Periplocoideae are sources of many indigenous Indian medicinal plants. We surveyed antioxidant properties and total phenolic and flavonoid contents of 15 samples, representing 12 Indian medicinal plant species from these subfamilies. Total antioxidant...

    journal_title:Natural product research

    pub_type: 杂志文章

    doi:10.1080/14786410802228827

    authors: Surveswaran S,Cai YZ,Xing J,Corke H,Sun M

    更新日期:2010-02-01 00:00:00

  • Polyphenol content in white table grape (Vitis Vinifera) berries of cultivar Italia: interactive effect of irrigation, delayed harvest and storage.

    abstract::Polyphenol concentrations were quantified by rapid resolution liquid chromatography/mass spectrometry in white table grape. The experimental vineyard was subjected to different kinds of water supply and supply rates. Samples from the same vineyards were also analysed after 6 weeks of storage in a refrigerator and afte...

    journal_title:Natural product research

    pub_type: 杂志文章

    doi:10.1080/14786419.2011.606623

    authors: Capriotti AL,Caruso G,Cavaliere C,Foglia P,Laganà A,Samperi R

    更新日期:2012-01-01 00:00:00

  • A new cinnamoylglycoflavonoid, antimycobacterial and antioxidant constituents from Heritiera littoralis leaf extracts.

    abstract::A new cinnamolyglycoflavonoid 3-cinnamoyltribuloside (1), its precursor tribuloside and two known flavonoid glycosides afzelin and astilbin were isolated from Heritiera littoralis Dryand (Sterculiaceae) ethanolic leaf extract. The dichloromethane leaf extract afforded two known pentacyclic triterpenoids, 3β-taraxerol ...

    journal_title:Natural product research

    pub_type: 杂志文章

    doi:10.1080/14786419.2013.863202

    authors: Christopher R,Nyandoro SS,Chacha M,de Koning CB

    更新日期:2014-01-01 00:00:00

  • Biological activities of ophiobolin K and 6-epi-ophiobolin K produced by the endophytic fungus Aspergillus calidoustus.

    abstract::Endophytic fungi represent ubiquitous microbial organisms able to live in the tissues of different plants around the world and represent a prolific source of bioactive metabolites. In the present study, the endophytic fungus Aspergillus calidoustus was isolated from the medicinal plant Acanthospermum australe (Asterac...

    journal_title:Natural product research

    pub_type: 杂志文章

    doi:10.1080/14786419.2015.1022777

    authors: de Carvalho CR,Vieira Mde L,Cantrell CL,Wedge DE,Alves TM,Zani CL,Pimenta RS,Sales Junior PA,Murta SM,Romanha AJ,Rosa CA,Rosa LH

    更新日期:2016-01-01 00:00:00

  • Studies on the chemical constituents of Phyllanthus emblica.

    abstract::Phytochemical investigations on Phyllanthus emblica have resulted in the isolation of the two new flavonoids, kaempferol-3-O-alpha-L-(6''-methyl)-rhamnopyranoside (1) and kaempferol-3-O-alpha-L-(6''-ethyl)-rhamnopyranoside (2). Their structures were determined on the basis of extensive spectroscopic studies including ...

    journal_title:Natural product research

    pub_type: 杂志文章

    doi:10.1080/14786410601124664

    authors: Habib-ur-Rehman,Yasin KA,Choudhary MA,Khaliq N,Atta-ur-Rahman,Choudhary MI,Malik S

    更新日期:2007-07-20 00:00:00

  • Bamboo leave extract ameliorated 12-O-tetradecanoylphorbol-13-acetate (TPA) induced ear inflammation by reducing MAP kinase levels and NF-κB activation in mice model.

    abstract::Sasa coreana Nakai (SCN) is a medicinal plant commonly used against inflammation. However, the underlined mechanisms against skin inflammation is poorly understood. The present study investigated the effects of SCN leave extract on ear inflammation. To this aim, six-week-old male ICR mice was subjected to 12-O-tetrade...

    journal_title:Natural product research

    pub_type: 杂志文章

    doi:10.1080/14786419.2020.1753053

    authors: Khan I,Dey DK,Lee JH,Kang SC

    更新日期:2020-04-14 00:00:00

  • Antioxidant xanthones from Cratoxylum cochinchinense.

    abstract::A new xanthone named cratoxylumxanthone A (1), together with five known compounds: dulcisxanthone B (2), alpha-mangostin (3), beta-mangostin (4), 2-geranyl-1,3,7-trihydroxy-4-(3-methylbut-2-enyl)xanthone (5) and tectochrystin (6), was isolated from Cratoxylum cochinchinense stems. The structure of new compound was cha...

    journal_title:Natural product research

    pub_type: 杂志文章

    doi:10.1080/14786410601102033

    authors: Phuwapraisirisan P,Udomchotphruet S,Surapinit S,Tip-Pyang S

    更新日期:2006-12-01 00:00:00

  • A new carotenoid glycoside from Rehmannia glutinosa.

    abstract::A new carotenoid glycoside, namely neo-rehmannioside (1), together with five known compounds, 6-O-seco-hydroxyaeginetoyl ajugol (2), oxyrehmaionoside B (3), ajugol (4), geniposidic acid (5) and geniposide (6) was isolated from the 95% ethanol extract of dry roots of Rehmannia glutinosa. The structure of the new compou...

    journal_title:Natural product research

    pub_type: 杂志文章

    doi:10.1080/14786419.2010.514268

    authors: Fu GM,Shi SP,Ip FC,Pang HH,Ip NY

    更新日期:2011-08-01 00:00:00

  • Unexpected formation of hydroxybiphenylmethane derivatives and some new observations on Labat test.

    abstract::An unexpected synthesis of symmetrical hydroxybiphenylmethanes involving the reaction of 2-hydroxyphenyl benzyl ketones with ethoxymethyl chloride has been observed. Some new interesting observations of Labat test on colorimetric detection of bichalconyloxy, bichalconyl and biflavonylmethanes having oxygenated ortho p...

    journal_title:Natural product research

    pub_type: 杂志文章

    doi:10.1080/14786410600917902

    authors: Kukreja G,Campiani G,Khurana JM,Joshi BP,Grover SK

    更新日期:2006-10-01 00:00:00

  • Trichosanthes dioica root possesses stimulant laxative activity in mice.

    abstract::Trichosanthes dioica Roxb. (Cucurbitaceae), named 'pointed gourd' in English, is a dioecious climber grown in India. This study was aimed at evaluating the laxative activity of the aqueous extract of T. dioica root (TDA) in Swiss albino mice. The laxative activity of TDA (100 and 200 mg kg(-1) body weight per os) was ...

    journal_title:Natural product research

    pub_type: 杂志文章

    doi:10.1080/14786419.2010.535161

    authors: Bhattacharya S,Haldar PK

    更新日期:2012-01-01 00:00:00

  • A new phthalide from the endophytic fungus Xylaria sp. GDG-102.

    abstract::A new phthalide derivative, xylarphthalide A (1), along with two known compounds (-)-5-carboxylmellein (2) and (-)-5-methylmellein (3), were isolated from the endophytic fungus Xylaria sp. GDG-102 cultured from the Chinese medicinal plant Sophora tonkinensis. Their structures were identified by MS and NMR experiments,...

    journal_title:Natural product research

    pub_type: 杂志文章

    doi:10.1080/14786419.2017.1311892

    authors: Zheng N,Yao F,Liang X,Liu Q,Xu W,Liang Y,Liu X,Li J,Yang R

    更新日期:2018-04-01 00:00:00

  • Activity-guided isolation of antioxidants from the leaves of Terminalia arjuna.

    abstract::An activity-guided isolation and purification process was used to identify the l,l-diphenyl-2-picrylhydrazyl (DPPH) free radical-scavenging components of the food plant (Terminalia arjuna) of Antheraea mylitta. Dry leaves of T. arjuna were extracted with different solvents and tested for their antioxidant activity aga...

    journal_title:Natural product research

    pub_type: 杂志文章

    doi:10.1080/14786419.2013.879304

    authors: Singh PP,Chauhan SM

    更新日期:2014-01-01 00:00:00

  • Chemical composition and antimicrobial activity of the essential oils of some species of Anthemis sect. Anthemis (Asteraceae) from Sicily.

    abstract::The chemical composition of the essential oils isolated from the aerial parts of Anthemis arvensis L. subsp. arvensis, Anthemis cretica subsp. messanensis (Brullo) Giardina & Raimondo and from flowers and leaves of Anthemis cretica subsp. columnae (Ten.) Frezén were determinated by GC-FID and GC-MS analyses. Torreyol ...

    journal_title:Natural product research

    pub_type: 杂志文章

    doi:10.1080/14786419.2017.1297444

    authors: Riccobono L,Maggio A,Bruno M,Spadaro V,Raimondo FM

    更新日期:2017-12-01 00:00:00

  • A new furofuran lignan from Piper terminaliflorum Tseng.

    abstract::The chemical investigation of whole plants Piper terminaliflorum Tseng led to the isolation of one new furofuran lignan, 7-methoxyasarinin (1), along with three known amide alkaloids (2-4) as N-3,5-dimethoxy-4-hydroxycinnamoylpyrrole (2), dihydropipercide (3) and 1-[(2E,4E,9E)-10-(3,4-Methylenedioxyphenyl)-2,4,9-undec...

    journal_title:Natural product research

    pub_type: 杂志文章

    doi:10.1080/14786419.2017.1350671

    authors: Liu T,Liang Q,Zhang XM,Huang SY,Xu WH

    更新日期:2018-02-01 00:00:00

  • Production of camptothecin by hairy roots and regenerated transformed shoots of Ophiorrhiza rugosa var. decumbens.

    abstract::Camptothecin (CPT), the derivatives of which are used clinically for the treatment of metastatic colon cancer, is isolated from intact plants that can be subjected to environmental fluctuations. In vitro cultures may be an alternate and continuous source for year-round production of CPT. Since CPT production by undiff...

    journal_title:Natural product research

    pub_type: 杂志文章

    doi:10.1080/14786419.2011.559469

    authors: Kamble S,Gopalakrishnan R,Eapen S

    更新日期:2011-10-01 00:00:00

  • Comparative neuroprotective effects of native curcumin and its galactomannoside formulation in carbofuran-induced neurotoxicity model.

    abstract::Toxicity of the pesticide carbofuran (CF) can be alleviated by curcumin, if not for its poor bioavailability. Hence, we investigated the effect of a bioavailable curcumin-galactomannan complex (CGM) on CF-induced neurotoxicity in rats in comparison to that of unformulated standard curcumin (CS). The CF (5 mg/kg b.wt/d...

    journal_title:Natural product research

    pub_type: 杂志文章

    doi:10.1080/14786419.2018.1514401

    authors: E R S,P P B,Nair SS,Maliakel B,Kuttan R,I M K

    更新日期:2020-05-01 00:00:00

  • Chemical constituents and antitumor activity from Paris polyphylla Smith var. yunnanensis.

    abstract::Eleven compounds were isolated from the rhizomes of Paris polyphylla Smith var. yunnanensis. Their structures were elucidated on the basis of rigorous 1D and 2D NMR experiments as well as comparison with the literature data. To the best of our knowledge, compound 1 was only predicted by UPLC/Q-TOF MSE and the NMR spec...

    journal_title:Natural product research

    pub_type: 杂志文章

    doi:10.1080/14786419.2016.1219861

    authors: Jing S,Wang Y,Li X,Man S,Gao W

    更新日期:2017-03-01 00:00:00

  • Evidence of cyanamide production in hairy vetch Vicia villosa.

    abstract::Cyanamide (NH(2)CN) has recently been isolated as a plant growth inhibitor from Vicia villosa, which is the first discovery of cyanamide from natural sources. To reveal the presence of the biosynthesized cyanamide in plants, 3.4 mM potassium ((15)N)nitrate was administered to 15- to 35-day-old plants of V. villosa, fr...

    journal_title:Natural product research

    pub_type: 杂志文章

    doi:10.1080/14786410500143583

    authors: Kamo T,Kato K,Hiradate S,Nakajima E,Fujii Y,Hirota M

    更新日期:2006-05-10 00:00:00

  • LC determination and pharmacokinetic study of the main phenolic components of Portulaca oleracea L. extract in rat plasma after oral administration.

    abstract::This study investigated the pharmacokinetics of hesperidin (HP), ferulic acid (FA) and p-coumaric acid (CA) in rat plasma after oral administration of Portulaca oleracea L. extract (POE). The plasma concentrations were determined by HPLC with vitexin-2″-O-rhamnoside (VR) as internal standard. The calibration curves we...

    journal_title:Natural product research

    pub_type: 杂志文章

    doi:10.1080/14786419.2011.648193

    authors: Cheng Z,Wang D,Zhang W,Du Y,Wang Y,Zhai Y,Ying X,Kang T

    更新日期:2012-01-01 00:00:00

  • Evaluation of chemical profile and antioxidant activity of Tripleurospermum insularum, a new species from Turkey.

    abstract::This article presents the very first phytochemical investigation on new species Tripleurospermum insularum Inceer & Hayırlıoglu-Ayaz. The volatile profile of odorous parts of the plant was analysed by GC/MS, and compounds were identified in headspace and essential oil obtained from aerial parts, representing 70.81% an...

    journal_title:Natural product research

    pub_type: 杂志文章

    doi:10.1080/14786419.2014.968156

    authors: Ćavar Zeljković S,Ayaz FA,Inceer H,Hayirlioglu-Ayaz S,Colak N

    更新日期:2015-02-01 00:00:00

  • A new phenol glycoside from Physalis angulata.

    abstract::A new phenol glycoside, physanguloside A (1), was isolated from Physalis angulata together with four known compounds. We report herein, for the first time, the presence of compounds 2-5 in the genus Physalis. The structures of all the compounds were established by NMR, IR, UV and HRESIMS spectroscopic analyses, and co...

    journal_title:Natural product research

    pub_type: 杂志文章

    doi:10.1080/14786419.2016.1269102

    authors: Sun CP,Nie XF,Kang N,Zhao F,Chen LX,Qiu F

    更新日期:2017-05-01 00:00:00

  • Suppression of MeIQ-induced SOS response by allylbenzenes from Asiasarum heterotropoides in the Salmonella typhimurium OY1001/1A2 umu test.

    abstract::Three allylbenzenes from Asiasarum heterotropoides, methyleugenol (1), elemicin (2) and gamma-asaron (3) showed suppressive effects on umu gene expression of the SOS response in the Salmonella typhimurium OY1001/1A2 umu test against the mutagen 2-amino-3,4-dimethylimidazo[4,5-f ]quinoline (MeIQ). Gene expression was s...

    journal_title:Natural product research

    pub_type: 杂志文章

    doi:10.1080/14786410500462629

    authors: Miyazawa M,Kohno G,Okuno Y,Oda Y

    更新日期:2006-06-01 00:00:00

  • Lycopus europaeus: phenolic fingerprint, antioxidant activity and antimicrobial effect on clinical Staphylococcus aureus strains.

    abstract::Lycopus europaeus L. leaves water extract (LEL) was subjected to phytochemical analysis, and evaluated for its antibacterial and antioxidant effects. Antibacterial activity testing was performed on Staphylococcus aureus clinical strains from catheter-related and skin infections by broth microdilution test. LEL showed ...

    journal_title:Natural product research

    pub_type: 杂志文章

    doi:10.1080/14786419.2015.1010086

    authors: Fialová S,Slobodníková L,Veizerová L,Grančai D

    更新日期:2015-01-01 00:00:00