Production of camptothecin by hairy roots and regenerated transformed shoots of Ophiorrhiza rugosa var. decumbens.

Abstract:

:Camptothecin (CPT), the derivatives of which are used clinically for the treatment of metastatic colon cancer, is isolated from intact plants that can be subjected to environmental fluctuations. In vitro cultures may be an alternate and continuous source for year-round production of CPT. Since CPT production by undifferentiated cell cultures is low, differentiated tissues such as root cultures may be a viable alternate source for CPT production. Hairy roots were induced in Ophiorriza rugosa, a source of CPT, using Agrobacterium rhizogenes strain LBA9402. The hairy roots, when cultured in light, showed spontaneous regeneration of shoots. Analysis of CPT levels in the hairy roots and in vitro-grown transformed shoots revealed 0.009% d.w. and 0.012% d.w., respectively.

journal_name

Nat Prod Res

journal_title

Natural product research

authors

Kamble S,Gopalakrishnan R,Eapen S

doi

10.1080/14786419.2011.559469

subject

Has Abstract

pub_date

2011-10-01 00:00:00

pages

1762-5

issue

18

eissn

1478-6419

issn

1478-6427

journal_volume

25

pub_type

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