Authentication of M14 melanoma cell line proves misidentification of MDA-MB-435 breast cancer cell line.

Abstract:

:A variety of analytical approaches have indicated that melanoma cell line UCLA-SO-M14 (M14) and breast carcinoma cell line MDA-MB-435 originate from a common donor. This indicates that at some point in the past, one of these cell lines became misidentified, meaning that it ceased to correspond to the reported donor and instead became falsely identified (through cross-contamination or other means) as a cell line from a different donor. Initial studies concluded that MDA-MB-435 was the misidentified cell line and M14 was the authentic cell line, although contradictory evidence has been published, resulting in further confusion. To address this question, we obtained early samples of the melanoma cell line (M14), a lymphoblastoid cell line from the same donor (ML14), and donor serum preserved at the originator's institution. M14 samples were cryopreserved in December 1975, before MDA-MB-435 cells were established in culture. Through a series of molecular characterizations, including short tandem repeat (STR) profiling and cytogenetic analysis, we demonstrated that later samples of M14 and MDA-MB-435 correspond to samples of M14 frozen in 1975, to the lymphoblastoid cell line ML14, and to the melanoma donor's STR profile, sex and blood type. This work demonstrates conclusively that M14 is the authentic cell line and MDA-MB-435 is misidentified. With clear provenance information and authentication testing of early samples, it is possible to resolve debates regarding the origins of problematic cell lines that are widely used in cancer research.

journal_name

Int J Cancer

authors

Korch C,Hall EM,Dirks WG,Ewing M,Faries M,Varella-Garcia M,Robinson S,Storts D,Turner JA,Wang Y,Burnett EC,Healy L,Kniss D,Neve RM,Nims RW,Reid YA,Robinson WA,Capes-Davis A

doi

10.1002/ijc.31067

subject

Has Abstract

pub_date

2018-02-01 00:00:00

pages

561-572

issue

3

eissn

0020-7136

issn

1097-0215

journal_volume

142

pub_type

杂志文章
  • Monoclonal antibodies to human colorectal epithelium: markers for differentiation and tumour characterization.

    abstract::A panel of monoclonal antibodies (MAbs) has been obtained, directed against determinants of normal human colorectal epithelium. BALB/c mice were immunized and boosted with mucosal scrapings and cell membrane preparations from normal large intestine. In one case boosting was also performed with HT29 colon carcinoma cel...

    journal_title:International journal of cancer

    pub_type: 杂志文章

    doi:10.1002/ijc.2910390309

    authors: Richman PI,Bodmer WF

    更新日期:1987-03-15 00:00:00

  • Expression of the macrophage antigen CD163 in rectal cancer cells is associated with early local recurrence and reduced survival time.

    abstract::Expression of the macrophage antigen CD163 in breast cancer cells is recently shown to be related to early distant recurrence and shortened survival. In this study, 163 patients with rectal cancer, included in the Swedish rectal cancer trial and followed up for a median of 71 months, were examined for the expression o...

    journal_title:International journal of cancer

    pub_type: 杂志文章,随机对照试验

    doi:10.1002/ijc.24506

    authors: Shabo I,Olsson H,Sun XF,Svanvik J

    更新日期:2009-10-15 00:00:00

  • Alcohol consumption, type of alcoholic beverage and risk of colorectal cancer at specific subsites.

    abstract::Within the Netherlands Cohort Study on diet and cancer, we investigated associations between total alcohol consumption, specific alcoholic beverage consumption and risk of colorectal cancer (CRC) according to anatomical subsite. Hazard Ratios (HR) and 95% confidence intervals (CI) were estimated using Cox proportional...

    journal_title:International journal of cancer

    pub_type: 杂志文章

    doi:10.1002/ijc.23774

    authors: Bongaerts BW,van den Brandt PA,Goldbohm RA,de Goeij AF,Weijenberg MP

    更新日期:2008-11-15 00:00:00

  • Laboratory determination of chemotherapeutic drug resistance in tumor cells from patients with leukemia, using a fluorometric microculture cytotoxicity assay (FMCA).

    abstract::An automated fluorometric microculture cytotoxicity assay (FMCA) based on the measurement of fluorescence generated from cellular hydrolysis of fluorescein diacetate (FDA) to fluorescein was employed for chemotherapeutic-drug-sensitivity testing of tumor-cell suspensions from patients with leukemia. Fluorescence was l...

    journal_title:International journal of cancer

    pub_type: 杂志文章

    doi:10.1002/ijc.2910500204

    authors: Larsson R,Kristensen J,Sandberg C,Nygren P

    更新日期:1992-01-21 00:00:00

  • Kinetics of the immune response of tumor-bearing hamsters to two simian virus 40 coded non-structural polypeptides present in simian virus 40 tumor cells.

    abstract::The kinetics of the immune response of hamsters transplanted with virus-free SV40 tumor cells (TSV5) to two SV40-coded non-structural proteins of 94,000 daltons (T antigen) and 17,000 daltons (t antigen) was studied in order to determine if the variation in the immune reactivity of serum from tumor-bearing animals tow...

    journal_title:International journal of cancer

    pub_type: 杂志文章

    doi:10.1002/ijc.2910290218

    authors: Ransom JH,Thompson DL,Tevethia SS

    更新日期:1982-02-15 00:00:00

  • Possible role of Cdx2 in the serrated pathway of colorectal cancer characterized by BRAF mutation, high-level CpG Island methylator phenotype and mismatch repair-deficiency.

    abstract::Colorectal cancer is a heterogeneous disease at the histomorphological, clinical and molecular level. Approximately 20% of cases may progress through the "serrated" pathway characterized by BRAF mutation and high-level CpG Island Methylator Phenotype (CIMP). A large subgroup are additionally microsatellite instable (M...

    journal_title:International journal of cancer

    pub_type: 杂志文章

    doi:10.1002/ijc.28564

    authors: Dawson H,Galván JA,Helbling M,Muller DE,Karamitopoulou E,Koelzer VH,Economou M,Hammer C,Lugli A,Zlobec I

    更新日期:2014-05-15 00:00:00

  • Urine estrogens and breast cancer risk factors among post-menopausal women.

    abstract::Creatinine-adjusted levels of estrone, estradiol and estriol were determined in overnight urine specimens from 220 post-menopausal women, aged 54 to 66 years, from Boston (121 women) and Athens (99 women). The associations of individual and total estrogens with various characteristics of the women (age at menopause, y...

    journal_title:International journal of cancer

    pub_type: 杂志文章

    doi:10.1002/ijc.2910400602

    authors: Trichopoulos D,Brown J,MacMahon B

    更新日期:1987-12-15 00:00:00

  • An in vitro model of intra-epithelial expansion of transformed urothelial cells.

    abstract::Replacement of normal urothelium by pre-cancerous epithelium may explain the high recurrence rate of human bladder cancer. An in vitro model was designed in order to study the mechanisms of expansion of transformed urothelial cells at the expense of normal urothelium. For this purpose, mouse bladder explants were allo...

    journal_title:International journal of cancer

    pub_type: 杂志文章

    doi:10.1002/ijc.2910540520

    authors: Rebel JM,de Boer WI,Thijssen CD,Vermey M,Zwarthoff EC,van der Kwast TH

    更新日期:1993-07-09 00:00:00

  • Induction of tubulin by docetaxel is associated with p53 status in human non small cell lung cancer cell lines.

    abstract::Docetaxel (DOC), a member of the taxane family of anticancer drugs, binds to tubulin and produces unnaturally stable microtubules that induce cell death. DOC is used clinically alone or in combination with other compounds to treat advanced stages of cancer. We have treated the human lung cancer cell lines A549 and H12...

    journal_title:International journal of cancer

    pub_type: 杂志文章

    doi:10.1002/ijc.21372

    authors: Chang JT,Chang GC,Ko JL,Liao HY,Liu HJ,Chen CC,Su JM,Lee H,Sheu GT

    更新日期:2006-01-15 00:00:00

  • Distribution of individual components of basement membrane in human colon polyps and adenocarcinomas as revealed by monoclonal antibodies.

    abstract::Double-label immunofluorescence was used to monitor basement-membrane composition and integrity in 22 human colon polyps, 36 adenocarcinomas and 2 metastases. Cryostat sections were stained with polyclonal anti-laminin anti-serum combined with monoclonal antibodies (MAbs) to all major basement-membrane components (lam...

    journal_title:International journal of cancer

    pub_type: 杂志文章

    doi:10.1002/ijc.2910500412

    authors: Ljubimov AV,Bartek J,Couchman JR,Kapuller LL,Veselov VV,Kovarik J,Perevoshchikov AG,Krutovskikh VA

    更新日期:1992-02-20 00:00:00

  • Long-term C-cell-mediated immunity to Epstein-Barr virus in man. II. Components necessary for regression in virus-infected leukocyte cultures.

    abstract::Regression of EB-virus-induced transformation occurs exclusively in cultures of leukocytes from seropositive donors. Studies have shown that the strength of regression could be assayed in terms of the proportion of T cells which must be added to the autologous EB virus-infected T-cell-depleted population in order to e...

    journal_title:International journal of cancer

    pub_type: 杂志文章

    doi:10.1002/ijc.2910230505

    authors: Rickinson AB,Moss DJ,Pope JH

    更新日期:1979-05-15 00:00:00

  • Natural killer cell activity associated with reticulum cell neoplasms.

    abstract::A marked increase in natural killer (NK) activity is observed with lymphoid cells prepared from SJL/J mouse spleen and lymph nodes, in which a transplantable reticulum-cell neoplasm (RCN) is growing. The killer cells are non-adherent, non-phagocytic, relatively resistant to X-ray, and scarcely or only partially inacti...

    journal_title:International journal of cancer

    pub_type: 杂志文章

    doi:10.1002/ijc.2910250316

    authors: Chang KS,Log T

    更新日期:1980-03-15 00:00:00

  • Chronic inflammation and risk of colorectal and other obesity-related cancers: The health, aging and body composition study.

    abstract::Evidence of the association between chronic inflammation and the risk of colorectal cancer (CRC) and other obesity-related cancers (OBRC) remains inconsistent, possibly due to a paucity of studies examining repeated measures of inflammation. In the Health ABC prospective study of 2,490 adults aged 70-79 years at basel...

    journal_title:International journal of cancer

    pub_type: 杂志文章

    doi:10.1002/ijc.29868

    authors: Izano M,Wei EK,Tai C,Swede H,Gregorich S,Harris TB,Klepin H,Satterfield S,Murphy R,Newman AB,Rubin SM,Braithwaite D,Health ABC study.

    更新日期:2016-03-01 00:00:00

  • Marine omega-3 fatty acid intake and survival of stage III colon cancer according to tumor molecular markers in NCCTG Phase III trial N0147 (Alliance).

    abstract::Marine omega-3 polyunsaturated fatty acids (MO3PUFAs) have anticancer properties and may improve colon cancer survival. However, it remains unknown whether the benefit differs by tumor molecular subtype. We examined data from a phase III randomized trial of FOLFOX or FOLFOX + cetuximab among 1,735 stage III colon canc...

    journal_title:International journal of cancer

    pub_type: 杂志文章,多中心研究,随机对照试验

    doi:10.1002/ijc.32113

    authors: Song M,Ou FS,Zemla TJ,Hull MA,Shi Q,Limburg PJ,Alberts SR,Sinicrope FA,Giovannucci EL,Van Blarigan EL,Meyerhardt JA,Chan AT

    更新日期:2019-07-15 00:00:00

  • siRNA gelsolin knockdown induces epithelial-mesenchymal transition with a cadherin switch in human mammary epithelial cells.

    abstract::Epithelial-mesenchymal transition (EMT) describes a process occurring during development and oncogenesis by which epithelial cells obtain fibroblast-like properties and show reduced cell adhesion and increased motility. In this report, we demonstrated typical EMT in human mammary epithelial MCF10A small interfering (s...

    journal_title:International journal of cancer

    pub_type: 杂志文章

    doi:10.1002/ijc.21559

    authors: Tanaka H,Shirkoohi R,Nakagawa K,Qiao H,Fujita H,Okada F,Hamada J,Kuzumaki S,Takimoto M,Kuzumaki N

    更新日期:2006-04-01 00:00:00

  • MicroRNA alterations in head and neck squamous cell carcinoma.

    abstract::MicroRNAs (mirs) are small noncoding RNA molecules (~22 nucleotides) that regulate posttranscriptional gene expression. Currently, there has not been a comprehensive study of their role in primary head and neck squamous cell carcinoma (HNSCC). To determine the role of mirs in HNSCC, we screened for altered microRNA ex...

    journal_title:International journal of cancer

    pub_type: 杂志文章

    doi:10.1002/ijc.23831

    authors: Chang SS,Jiang WW,Smith I,Poeta LM,Begum S,Glazer C,Shan S,Westra W,Sidransky D,Califano JA

    更新日期:2008-12-15 00:00:00

  • Whole exome sequencing identifies PLEC, EXO5 and DNAH7 as novel susceptibility genes in testicular cancer.

    abstract::Testicular germ cell tumors (TGCTs) are a clinically and pathologically heterogeneous disease, and little is known of its genetic basis. Only low susceptibility risk loci have been identified for both sporadic and familial cases. Therefore, we tried to identify new susceptibility genes responsible for familial testicu...

    journal_title:International journal of cancer

    pub_type: 杂志文章

    doi:10.1002/ijc.31604

    authors: Paumard-Hernández B,Calvete O,Inglada Pérez L,Tejero H,Al-Shahrour F,Pita G,Barroso A,Carlos Triviño J,Urioste M,Valverde C,González Billalabeitia E,Quiroga V,Francisco Rodríguez Moreno J,Fernández Aramburo A,López C,Maroto

    更新日期:2018-10-15 00:00:00

  • Losses of the tumor suppressor BIN1 in breast carcinoma are frequent and reflect deficits in programmed cell death capacity.

    abstract::Oncogenic activation of MYC occurs often in breast carcinoma and is associated with poor prognosis. Loss or inactivation of mechanisms that restrain MYC may therefore be involved in tumor progression. In this study, we show that the MYC-interacting adaptor protein BIN1 is frequently missing in malignant breast cells a...

    journal_title:International journal of cancer

    pub_type: 杂志文章

    doi:

    authors: Ge K,Duhadaway J,Sakamuro D,Wechsler-Reya R,Reynolds C,Prendergast GC

    更新日期:2000-02-01 00:00:00

  • Features of gastric cancer in hereditary non-polyposis colorectal cancer syndrome.

    abstract::To identify characteristics of gastric cancer associated with hereditary non-polyposis colorectal cancer (HNPCC), we gathered clinical data and tumor samples relating to patients recorded in the Finnish HNPCC registry. Our series included 51 families with a characterized mutation and/or that met the Amsterdam criteria...

    journal_title:International journal of cancer

    pub_type: 杂志文章

    doi:10.1002/(sici)1097-0215(19971021)74:5<551::aid-ijc

    authors: Aarnio M,Salovaara R,Aaltonen LA,Mecklin JP,Järvinen HJ

    更新日期:1997-10-21 00:00:00

  • Differential expression of two lck transcripts directed from the distinct promoters in HTLV-I+ and HTLV-I- T-cells.

    abstract::The lck gene encodes a lymphocyte-specific tyrosine protein kinase, p56lck, the expression of which is almost exclusive in T-cells. The expression of lck in human T-cell leukemia virus type I (HTLV-I)-transformed T-cell lines is closely associated with interleukin-2 (IL-2) dependence for their growth. That is, IL-2-de...

    journal_title:International journal of cancer

    pub_type: 杂志文章

    doi:10.1002/ijc.2910480526

    authors: Nakamura K,Koga Y,Yoshida H,Kimura G,Nomoto K

    更新日期:1991-07-09 00:00:00

  • Profiling the humoral immune response in colon cancer patients: diagnostic antigens from Streptococcus bovis.

    abstract::The human bowel contains a large and dynamic bacterial population that is not only essential for intestinal health, but also critical for the development of diseases such as cancer. In this respect, the Gram-positive bacterium Streptococcus bovis has been associated with colon cancer for many years. To investigate the...

    journal_title:International journal of cancer

    pub_type: 杂志文章

    doi:10.1002/ijc.22116

    authors: Tjalsma H,Schöller-Guinard M,Lasonder E,Ruers TJ,Willems HL,Swinkels DW

    更新日期:2006-11-01 00:00:00

  • Downregulation of SERPINB13 expression in head and neck squamous cell carcinomas associates with poor clinical outcome.

    abstract::Tumorigenesis of head and neck squamous cell carcinomas (HNSCC) is associated with various genetic changes such as loss of heterozygosity (LOH) on human chromosome 18q21. This chromosomal region maps a gene cluster coding for a family of intracellular serine protease inhibitors (serpins), including SERPINB13. As SERPI...

    journal_title:International journal of cancer

    pub_type: 杂志文章

    doi:10.1002/ijc.24507

    authors: de Koning PJ,Bovenschen N,Leusink FK,Broekhuizen R,Quadir R,van Gemert JT,Hordijk GJ,Chang WS,van der Tweel I,Tilanus MG,Kummer JA

    更新日期:2009-10-01 00:00:00

  • Inhibition of stromal matrix metalloproteases: effects on breast-tumor promotion by fibroblasts.

    abstract::Co-injection of fibroblasts with human epithelial breast-tumor MCF7 cells in the presence of Matrigel enhances tumor growth in nude mice. While most of the matrix metalloproteinases (MMPs) have been shown to be produced by stromal cells, tumor cells such as MCF7 cells are unable to produce MMPs. We therefore, hypothes...

    journal_title:International journal of cancer

    pub_type: 杂志文章

    doi:10.1002/(sici)1097-0215(19980413)76:2<267::aid-ijc

    authors: Noël A,Hajitou A,L'Hoir C,Maquoi E,Baramova E,Lewalle JM,Remacle A,Kebers F,Brown P,Calberg-Bacq CM,Foidart JM

    更新日期:1998-04-13 00:00:00

  • Production of fibronectin by human tumor cells and interaction with exogenous fibronectin: comparison of cell lines obtained from colon adenocarcinomas and squamous carcinomas of the upper aerodigestive tract.

    abstract::Cell lines derived from 13 different human colon adenocarcinomas were examined for production of fibronectin by ELISA and for cell-surface expression of fibronectin by indirect immunofluorescence. Two squamous epithelial cell lines obtained from tumors of the upper aerodigestive tract were used as controls. None of th...

    journal_title:International journal of cancer

    pub_type: 杂志文章

    doi:10.1002/ijc.2910470319

    authors: Varani J,Schuger L,Fligiel SE,Inman DR,Chakrabarty S

    更新日期:1991-02-01 00:00:00

  • Occupation and risk of Hodgkin's disease in north-east Italy.

    abstract::The relationship between occupation and exposure to several occupational agents and Hodgkin's disease (HD) was investigated in a case-control study of 152 cases and 613 controls with acute diseases admitted to all hospitals in Pordenone province, north-east Italy. Among cases, there was a significant excess of individ...

    journal_title:International journal of cancer

    pub_type: 杂志文章

    doi:10.1002/ijc.2910480608

    authors: Franceschi S,Serraino D,La Vecchia C,Bidoli E,Tirelli U

    更新日期:1991-07-30 00:00:00

  • Dietary sources of N-nitroso compounds and bladder cancer risk: findings from the Los Angeles bladder cancer study.

    abstract::N-Nitroso compounds (NOCs) have been proposed as possible bladder carcinogens. The main sources of exogenous exposure to NOCs are cigarette smoke and diet, particularly processed (i.e., nitrite-treated) meats. Perhaps more importantly, NOCs can be formed endogenously from dietary precursors such as nitrate, nitrite an...

    journal_title:International journal of cancer

    pub_type: 杂志文章

    doi:10.1002/ijc.28331

    authors: Catsburg CE,Gago-Dominguez M,Yuan JM,Castelao JE,Cortessis VK,Pike MC,Stern MC

    更新日期:2014-01-01 00:00:00

  • Statin use and mortality among ovarian cancer patients: A population-based cohort study.

    abstract::Statin use has been suggested to improve prognosis in cancer patients, however, for ovarian cancer, the evidence is sparse. From the Danish Cancer Registry, we identified patients aged 30-84 years with a histologically verified first diagnosis of epithelial ovarian cancer between 2000 and 2013. Data on filled prescrip...

    journal_title:International journal of cancer

    pub_type: 杂志文章

    doi:10.1002/ijc.30738

    authors: Verdoodt F,Kjaer Hansen M,Kjaer SK,Pottegård A,Friis S,Dehlendorff C

    更新日期:2017-07-15 00:00:00

  • Tumor eradication by immunotherapy with biodegradable PLGA microspheres--an alternative to incomplete Freund's adjuvant.

    abstract::In experimental tumor immunotherapy, incomplete Freund's adjuvant (IFA) has been considered as the "gold standard" for T-cell vaccination in mice and humans in spite of its considerable adverse effects. Recently, we succeeded in eliciting strong CTL responses in mice after vaccination with biodegradable poly(D,L-lacti...

    journal_title:International journal of cancer

    pub_type: 杂志文章

    doi:10.1002/ijc.25914

    authors: Mueller M,Schlosser E,Gander B,Groettrup M

    更新日期:2011-07-15 00:00:00

  • Human anti-idiotypic antibodies can be good immunogens as they target FC receptors on antigen-presenting cells allowing efficient stimulation of both helper and cytotoxic T-cell responses.

    abstract::Anti-idiotypic antibodies that mimic tumour-associated antigens can stimulate anti-tumour T-cell responses. In this article, we have studied the role of Fc in the presentation of T-cell epitopes by 2 anti-idiotypic antibodies, 105AD7 and 708. The human monoclonal antibody 105AD7, which mimics CD55, stimulated strong i...

    journal_title:International journal of cancer

    pub_type: 杂志文章

    doi:10.1002/ijc.1194

    authors: Durrant LG,Parsons T,Moss R,Spendlove I,Carter G,Carr F

    更新日期:2001-05-01 00:00:00

  • Role of reproductive factors on the risk of endometrial cancer.

    abstract::We analyzed the role of reproductive factors on the risk of endometrial cancer using data from a case-control study conducted in Italy. Cases were 752 women with histologically confirmed endometrial cancer < 75 years of age. Controls were 2,606 patients < 75 years of age admitted for acute, non-gynecological non-hormo...

    journal_title:International journal of cancer

    pub_type: 杂志文章

    doi:10.1002/(sici)1097-0215(19980610)76:6<784::aid-ijc

    authors: Parazzini F,Negri E,La Vecchia C,Benzi G,Chiaffarino F,Polatti A,Francheschi S

    更新日期:1998-06-10 00:00:00