Abstract:
:Cell lines derived from 13 different human colon adenocarcinomas were examined for production of fibronectin by ELISA and for cell-surface expression of fibronectin by indirect immunofluorescence. Two squamous epithelial cell lines obtained from tumors of the upper aerodigestive tract were used as controls. None of the 13 colon carcinoma lines produced detectable amounts of fibronectin or showed detectable cell-surface staining with anti-fibronectin. The 2 squamous epithelial cell lines, in contrast, produced large amounts of fibronectin which could be detected in the culture medium and bound to the substratum. The squamous carcinoma cells also stained brightly when examined in the viable state by immunofluorescence with anti-fibronectin. In addition to being studied for fibronectin production, each cell line was also examined for the ability to interact with exogenous fibronectin in an adhesion assay. None of the colon carcinoma cells were adherent to fibronectin-coated culture dishes while the 2 squamous carcinoma cells rapidly attached and spread on this substratum. These data suggest that cell lines derived from adenocarcinomas of the colon are deficient in production of fibronectin and in their ability to interact with exogenous fibronectin. In their degree of deficiency, the colon carcinoma cells are significantly different from several different types of human tumor cell. The failure of the colon carcinoma cells to synthesize detectable amounts of fibronectin endogenously or to interact with exogenous fibronectin may explain, in part, the low degree of adhesive interaction which these cells have for their substratum. This, in turn, may influence the in vitro and in vivo properties of colon carcinoma cells.
journal_name
Int J Cancerjournal_title
International journal of cancerauthors
Varani J,Schuger L,Fligiel SE,Inman DR,Chakrabarty Sdoi
10.1002/ijc.2910470319subject
Has Abstractpub_date
1991-02-01 00:00:00pages
421-5issue
3eissn
0020-7136issn
1097-0215journal_volume
47pub_type
杂志文章abstract::Non-steroidal anti-inflammatory drugs (NSAIDs) inhibit proliferation and angiogenesis in colorectal cancer. We examined a possible involvement of store-operated calcium (SOC) entry in human colon carcinoma cells (HRT-18), which require calcium for proliferation. Acetyl-salicylic-acid (ASA), mefenamic acid (MEF) and su...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.1280
更新日期:2001-06-15 00:00:00
abstract::The purpose of our study was to investigate a novel therapeutic approach for rhabdomyosarcoma (RMS) in an animal model. The pursuit of new therapeutic modalities for RMS is critically important since this type of tumor is the most common soft tissue sarcoma in children and because patients with metastatic disease may ...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/(SICI)1097-0215(19961127)68:5<596::AID-IJC
更新日期:1996-11-27 00:00:00
abstract::The 9p21-23 chromosome region harbors a number of known and putative tumor-suppressor genes (TSGs). The best characterized gene in this area is p16(INK4A) (CDKN2A). Alterations of its product have been observed in various malignancies, including non-small-cell lung carcinomas (NSCLCs). We earlier investigated the mech...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/(sici)1097-0215(20000320)89:2<133::aid-ijc
更新日期:2000-03-20 00:00:00
abstract::The heterogeneous nuclear ribonucleoprotein (hnRNP) K is an essential RNA and DNA binding protein involved in gene expression and signal transduction including DNA transcription, RNA splicing, RNA stability and translation. The role of hnRNP K in cancer is relatively understudied. However, several cellular functions s...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.24744
更新日期:2010-01-15 00:00:00
abstract::Cytokines play a crucial role in tumor initiation and progression. Here, we demonstrate that interleukin (IL)-6 is a key factor by driving tumor progression from benign to malignant, invasive tumors in the HaCaT-model of human skin carcinoma. IL-6 activates STAT3 and directly stimulates proliferation and migration of ...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.25621
更新日期:2011-06-15 00:00:00
abstract::Morphologic and metabolic studies have been carried out on Friend leukemia cell (FLC) tumors (grown in DBA/2 mice) or fibrosarcomas (grown in C3H/HeN or C3H/HeJ mice) shortly after peritumoral injection of recombinant mouse tumor necrosis factor (TNF) alpha. Marked vascular congestion and focal extravasation of erythr...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.2910420420
更新日期:1988-10-15 00:00:00
abstract::A population-based case-control study of incident primary brain tumors in adults was carried out in Adelaide, Australia in the period 1987 through 1990. It included 110 subjects with newly diagnosed primary glioma, 60 subjects with meningioma and 417 controls selected from the Australian Electoral Roll and frequency-m...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.2910510105
更新日期:1992-04-22 00:00:00
abstract::The tumor site is a strong clinical factor in head and neck squamous cell carcinoma (HNSCC). To clarify the biologic and clinical role of p53 alterations in HNSCC, we have examined the prevalence and the nature of p53 alterations in a large cohort of tumors from the different sites. For immunohistochemical analysis of...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.11698
更新日期:2004-09-10 00:00:00
abstract::Lifestyle factors and medical history are known to influence risk of non-Hodgkin lymphoma (NHL). Whether these factors affect the prognosis of NHL, especially its subtypes, is unclear. To investigate this, the association between these factors and all-cause and lymphoma-related mortality was assessed in a population-b...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.27944
更新日期:2013-06-01 00:00:00
abstract::CD16 surface antigens represent activatory molecules in CD3-16+ NK cells. In order to target NK cells against relatively NK-resistant ovarian carcinomas, we used an anti-CD16 monoclonal antibody (MAb) (VD4), together with an anti-ovarian carcinoma-associated antigen (MOV19), to construct biMAbs. To this end, hybrid hy...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.2910480213
更新日期:1991-05-10 00:00:00
abstract::We, and others, have recently shown that recombinant feline leukaemia viruses (FeLV) containing the myc gene (FeLV-myc) occur in up to 30% of naturally occurring cases of T-cell lymphosarcoma. Investigation of the disease spectrum of two FeLV-myc isolates showed that they induced clonal or oligoclonal T-cell tumours a...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.2910400108
更新日期:1987-07-15 00:00:00
abstract::Colon cancer tissues display an increased activity of beta-galactoside alpha2,6 sialyltransferase (ST6Gal.I) and an increased reactivity with the lectin from Sambucus nigra (SNA), specific for alpha2,6-sialyl-linkages. Experimental and clinical studies indicate a contribution of these alterations to tumor progression,...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/1097-0215(20001001)88:1<58::aid-ijc9>3.0.c
更新日期:2000-10-01 00:00:00
abstract::Adhesion molecules involved in attachment between human pancreatic carcinoma and activated endothelial cells in vitro were investigated. Basal adhesion occurred between 6 pancreatic carcinoma cell lines and unstimulated human umbilical vein endothelial cells (HUVEC), and augmented basal adhesion to activated HUVEC was...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.2910540618
更新日期:1993-07-30 00:00:00
abstract::Colorectal cancer (CRC) is the third most common cancer and the second leading cause of cancer mortality worldwide. Emerging evidence indicates that tumour cells release substantial amounts of RNA into the bloodstream, in which RNA strongly resists RNases and is present at sufficient levels for quantitative analyses. ...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.32747
更新日期:2020-05-15 00:00:00
abstract::V-ATPases are proton-translocating enzymes, which are found not only in numerous intracellular organelles but also in the plasma membranes of many eukaryotic cells. Using differential display, we have identified one of the proton pump subunit genes, ATP6C, as a cisplatin-inducible gene. Northern blot analysis demonstr...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.1418
更新日期:2001-09-01 00:00:00
abstract::A new human cell line, designated Ty-82, was established from the pleural effusion of a 22-year-old woman with undifferentiated thymic carcinoma. This cell line consisted of primitive cells that were positive for alpha-naphthyl butyrate esterase and acid phosphatase. The cells were shown to express epithelial membrane...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.2910500216
更新日期:1992-01-21 00:00:00
abstract::The potential role of p16(INK4a) methylation in breast cancer is controversial whereas there are no data on fibroadenoma. To assess if inactivation of p16(INK4a) by promoter hypermethylation occurs in this hyperproliferative benign breast lesion or, on the contrary, it is strictly related to the carcinogenic process, ...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.20771
更新日期:2005-04-10 00:00:00
abstract::TAAs of the MAGE family are mostly studied as targets of specific immune responses. Their potential relevance as tumor markers has also been underlined. We used a MAb, 57B, recognizing MAGE-A4 protein in paraffin-embedded sections, to evaluate its expression in bladder cancers by employing TMA including 2,317 samples ...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.10540
更新日期:2002-08-20 00:00:00
abstract::Targeting of T lymphocytes against epidermal growth-factor-receptor (EGF-R)+ tumor cells was achieved by constructing a hybrid hybridoma which secretes an anti-EGF-R/anti-CD3 bispecific monoclonal antibody (biMAb) of hybrid isotype (IgG1/IgG2a). Purification of biMAb molecules from parental anti-EGF-R and anti-CD3 MAb...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.2910550610
更新日期:1993-12-02 00:00:00
abstract::Invasion of malignant cells requires altered cellular interaction with extracellular matrix. Integrin-type cell adhesion receptors play an important role in this process. Integrin-related cell biological phenomena explain cancer cell migration, and recent developments in the field have made it possible to propose that...
journal_title:International journal of cancer
pub_type: 杂志文章,评审
doi:10.1002/(SICI)1097-0215(19960315)65:6<717::AID-IJC
更新日期:1996-03-15 00:00:00
abstract::Telomerase activity is involved in the maintenance of telomere length and is thought to be required for cellular immortality and oncogenesis. Three major subunits composing telomerase, human telomerase RNA (hTR), telomerase-associated protein (TPI) and human telomerase catalytic subunit (hTERT), have been identified. ...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/(sici)1097-0215(19990315)80:6<804::aid-ijc
更新日期:1999-03-15 00:00:00
abstract::An experimental rabbit model was used to determine host responses to infection by various human T-lymphotropic virus type-I (HTLV-I) strains. Seven groups of 4 to 5 rabbits each were inoculated with lethally-irradiated HTLV-I-infected cell lines derived from patients with adult T-cell leukemia/lymphoma or from patient...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.2910500125
更新日期:1992-01-02 00:00:00
abstract::The effects of DL-alpha-difluoromethylornithine (DFMO), a specific irreversible inhibitor of ornithine decarboxylase, on the growth of experimental mouse B16-F10 melanoma cells were investigated. DFMO (3%) in drinking water was administered to B16-F10 melanoma-bearing mice. At 24 days, B16-F10 melanomas in DFMO-fed mi...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.2910390220
更新日期:1987-02-15 00:00:00
abstract::alpha beta+ TCR T cells recognize peptide fragments displayed by MHC-class I or -class II molecules. Recently, additional mechanisms of antigen recognition by T cells have been identified, including CD1-mediated presentation of nonpeptide antigens. Only a limited number of CD1 antigens is retained in the mouse, i.e., ...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.10141
更新日期:2002-03-20 00:00:00
abstract::Activity of Janus kinase 2 (JAK2) in the JAK2/STAT5 signaling pathway is critically controlled by suppressor of cytokine signaling-1 (SOCS-1). We have previously shown that SOCS-1 is biallelically mutated in the primary mediastinal B-cell lymphoma (PMBL) cell line MedB-1, resulting in impaired JAK2 degradation and sus...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.21485
更新日期:2006-04-15 00:00:00
abstract::Eleven population-based cancer registries tabulated second cancers among 133,411 patients diagnosed with testicular cancer, ovarian cancer or Hodgkin's disease between 1945 and 1984. Overall, 3,157 second cancers were observed, as compared with 2,420 expected at least one year after the first cancer. Survivors of test...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.2910390506
更新日期:1987-05-15 00:00:00
abstract::HER2 is an erbB/HER type 1 tyrosine kinase receptor that is frequently over-expressed in malignant epithelial tumours. Herceptin, a humanised mouse monoclonal antibody to HER2, is proven therapeutically in the management of metastatic breast cancer, significantly prolonging survival when combined with cytotoxic chemot...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.1214
更新日期:2001-05-15 00:00:00
abstract::The association between breast cancer risk defined by the Tyrer-Cuzick score (TC) and disease prognosis is not well established. Here, we investigated the relationship between 5-year TC and disease aggressiveness and then characterized underlying molecular processes. In a case-only study (n = 2474), we studied the ass...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.33270
更新日期:2021-02-15 00:00:00
abstract::An antiserum to SF antigen was shown to be selectively cytotoxic to two human fibroblast lines, while two tumor cell lines (one melanoma and one Hela cell line) were resistant. The selective cytotoxicity was also demonstrable in mixtures of fibroblasts and tumor cells. The cytotoxic effect was abrogated by admixture o...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.2910180317
更新日期:1976-09-15 00:00:00
abstract::The p53 tumor suppressor pathway is impaired in more than 90% of cervical cancers and cancer-derived cell lines as a result of infection by human papillomavirus (HPV). The HPV E6 oncoprotein forms complexes with p53 and promotes its degradation via ubiquitin-dependent mechanism. In our study, we attempted to improve t...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.26013
更新日期:2012-01-15 00:00:00