Abstract:
:Tumorigenesis of head and neck squamous cell carcinomas (HNSCC) is associated with various genetic changes such as loss of heterozygosity (LOH) on human chromosome 18q21. This chromosomal region maps a gene cluster coding for a family of intracellular serine protease inhibitors (serpins), including SERPINB13. As SERPINB13 expression in HNSCC has recently been shown to be downregulated both at the mRNA and protein levels, here we investigated if such a low SERPINB13 expression is associated with histopathological and clinical parameters of HNSCC tumors and patient survival. By generating specific antibodies followed by immunohistochemistry on a well-defined cohort of 99 HNSCC of the oral cavity and oropharynx, SERPINB13 expression was found to be partially or totally downregulated in 75% of the HNSCC as compared with endogenous expression in non-neoplastic epithelial cells. Downregulation of SERPINB13 protein expression in HNSCC was significantly associated with the presence of LOH at the SERPINB13 gene in the tumors (p = 0.006), a poor differentiation grade of the tumors (p = 0.001), the presence of a lymph node metastasis (p = 0.012), and a decreased disease-free (p = 0.033) as well as overall (p = 0.018) survival of the patients. This is the first report demonstrating that downregulation of SERPINB13 protein expression in HNSCC is positively associated with poor clinical outcome. Therefore, SERPINB13 seems to act as an important protease inhibitor involved in the progression of HNSCC.
journal_name
Int J Cancerjournal_title
International journal of cancerauthors
de Koning PJ,Bovenschen N,Leusink FK,Broekhuizen R,Quadir R,van Gemert JT,Hordijk GJ,Chang WS,van der Tweel I,Tilanus MG,Kummer JAdoi
10.1002/ijc.24507subject
Has Abstractpub_date
2009-10-01 00:00:00pages
1542-50issue
7eissn
0020-7136issn
1097-0215journal_volume
125pub_type
杂志文章abstract::Owing to their role in controlling the efflux of toxic compounds, transporters are central players in the process of detoxification and elimination of xenobiotics, which in turn is related to cancer risk. Among these transporters, ATP-binding cassette B1/multidrug resistance 1 (ABCB1/MDR1), ABCC2/multidrug resistance ...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.26436
更新日期:2012-08-15 00:00:00
abstract::IL-2 gene was introduced through retroviral vectors into the highly malignant and poorly immunogenic 3LL-D122 clone. Both high and low D122-IL-2 secretors showed elimination of tumorigenicity in syngeneic immune-competent mice; however, in nude mice only the high IL-2 secretor showed reduced tumorigenicity as compared...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.2910530320
更新日期:1993-02-01 00:00:00
abstract::Thrombin is now recognized as an important factor in many cancers. Here, we examined the expression and role of the recently discovered thrombin receptor PAR4, in human colon cancer cells. PAR4 mRNA was found in 10 out of 14 (71%) human colon cancer cell lines tested but not in epithelial cells isolated from normal hu...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.24070
更新日期:2009-04-01 00:00:00
abstract::The morphology of rat liver cells containing alpha-feto-protein (AFP) during early stages of carcinogenesis induced by 3'methyl-4-dimethylaminoazobenzene and 2-acetylaminofluorene was investigated. Indirect immunofluorescence was used to detect AFP. AFP was not found in the cells of hyperplastic nodules but was presen...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.2910200310
更新日期:1977-09-15 00:00:00
abstract::The ability to monitor breast cancer initiation and progression on the molecular level would provide an effective tool for early diagnosis and therapy. In the present study, we focused on the underglycosylated MUC-1 tumor antigen (uMUC-1), which is directly linked to tumor progression from pre-malignancy to advanced m...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.27872
更新日期:2013-04-15 00:00:00
abstract::Syrian-hamster-derived lymphotoxin inhibits benzo(a)pyrene and ultraviolet carcinogen-induced in vitro morphologic transformation of diploid secondary passage hamster embryo cells. When lymphotoxin is added up to 4 days post carcinogen, the reduction in the frequency of morphologic transformation 6 days after carcinog...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.2910270108
更新日期:1981-01-15 00:00:00
abstract::Insulin-like growth factor-binding protein 7 (IGFBP7) functions in several cellular processes including proliferation, senescence and apoptosis. This study analyzed IGFBP7 function in hepatocellular carcinoma (HCC) cells by gene manipulation and investigated the prognostic significance of IGFBP7 expression in clinical...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.25994
更新日期:2012-01-15 00:00:00
abstract::Primary cultures derived from pre-cancerous and cancerous human colon tissue are essential for understanding normal and abnormal growth function in the large intestine. Here presented are (i) the methodology for routine establishment of primary cultures of normal, adenoma- and carcinoma-derived cells, and (ii) data fo...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/(sici)1097-0215(19980130)75:3<467::aid-ijc
更新日期:1998-01-30 00:00:00
abstract::Barrett's esophagus (BE) is a metaplastic condition caused by chronic gastroesophageal reflux which represents an early step in the development of esophageal adenocarcinoma (EAC). Single-nucleotide polymorphism microarray (SNP-chip) analysis is a novel, precise, high-throughput approach to examine genomic alterations ...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.24620
更新日期:2009-11-15 00:00:00
abstract::Analysis of the length of circulating DNA in plasma has been reported as a marker for solid tumor detection. We assessed the sensitivity and specificity of increased plasma DNA length to identify patients with head and neck squamous cell carcinoma (HNSCC) and monitor posttreatment disease status. Fifty-eight HNSCC pat...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.22250
更新日期:2006-12-01 00:00:00
abstract::A series of 42 patients with malignant melanoma treated with BCG adjuvant immunotherapy were studied for sequential changes in cellular immune reactivity to non-specific mitogens. Lymphocyte preparations were made monthly and stored in a viable condition in liquid nitrogen. After 6 months of treatment, all lymphocyte ...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.2910190104
更新日期:1977-01-01 00:00:00
abstract::The c-met oncogene encodes the receptor for hepatocyte growth factor/scatter factor (HGF/SF), a multifunctional cytokine able to mediate morphogenesis as well as mitogenesis, motogenesis and invasiveness of epithelial cells. HGF/SF has been implicated in branching tubulogenesis of the developing kidney and in regenera...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/(SICI)1097-0215(19960621)69:3<212::AID-IJC
更新日期:1996-06-21 00:00:00
abstract::Using immunohistochemical methods, we analyzed the association between nuclear p53 overexpression and various clinicopathological parameters in patients with endometrial cancers. Formalin-fixed and paraffin-embedded tissue sections from 139 cases of endometrial cancer (endometrioid type, 126; serous papillary type, 12...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.2910580104
更新日期:1994-07-01 00:00:00
abstract::Hereditary non-polyposis colorectal cancer, HNPCC, is an autosomal dominant condition predisposing to cancers of primarily the colorectum and the endometrium. The aim of our study was to identify persons at a high risk of hereditary colorectal cancer and to estimate their risk of colon and other HNPCC-associated tumou...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/1097-0215(200002)9999:9999<::aid-ijc1093>3
更新日期:2001-02-15 00:00:00
abstract::The modulating effect on drug resistance of amiodarone (AM) and its metabolite desethylamiodarone (DEA) was studied in a P-glycoprotein-positive human colon carcinoma cell line COLO 320, and a human small-cell lung carcinoma cell line GLC4 and its adriamycin (Adr)-resistant subline GLC4-Adr (both P-glycoprotein-negati...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.2910480422
更新日期:1991-06-19 00:00:00
abstract::Prospective cohort studies about cutaneous melanoma (CM) risk are still few. Host factor- and UVR exposure data were collected prospectively by questionnaire in this population-based cohort study including 40,000 Swedish born women, aged 25-64 years at enrolment (1990). Risk for CM (Cox regression and Stepwise Cox reg...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.26408
更新日期:2012-08-01 00:00:00
abstract::Paraffin sections from 190 epithelial ovarian tumours, including 159 malignant and 31 benign epithelial tumours, were analysed immunohistochemically for expression of cyclin-dependent kinase inhibitor 2 (CDKN2A) gene product p16INK4A (p16). Most benign tumours showed no p16 expression in the tumour cells, whereas only...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/(sici)1097-0215(19970220)74:1<57::aid-ijc1
更新日期:1997-02-20 00:00:00
abstract::Folate receptor-alpha (FR-alpha) is generally over-expressed in non-mucinous human ovarian carcinomas. The meaning of FR-alpha over-expression and its role in the 5-methyltetrahydrofolic acid (N5-CH3-H4PteGlu) transport in such tumors is not clear, especially compared with the reduced folate carrier (RFC), the other k...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/(sici)1097-0215(19980105)75:1<125::aid-ijc
更新日期:1998-01-05 00:00:00
abstract::Flavone acetic acid (FAA) is a potentially useful anti-tumour agent which has been reported to induce changes in tumour vasculature, in particular loss of bloodflow. This led us to examine whether endothelium could be a cellular target of FAA action, with resultant modulation of cell-surface coagulant properties leadi...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.2910490219
更新日期:1991-09-09 00:00:00
abstract::The phorbol esters phorbol 12-retinoate 13-acetate (RPA) and 12-O-tetradecanoyl phorbol 13-acetate (TPA) were used to investigate the role of tumor promoters in the control of hormone response in normal and leukemic myeloid cells. RPA and TPA inhibited the binding of [20-3H]phorbol 12,13-dibutyrate to the leukemic cel...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.2910310416
更新日期:1983-04-15 00:00:00
abstract::Using a direct binding liquid competition radioimmunoassay, the amount of the ras oncogene protein product, p21, was quantitated in a variety of human tumors and adjacent apparently normal tissues. In 48 of 50 matched tumor and normal tissue biopsy specimens from 50 patients, more ras p21 was detected in the tumor tha...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.2910430315
更新日期:1989-03-15 00:00:00
abstract::von Willebrand factor (vWF), a glycoprotein produced uniquely by endothelial cells and megakaryocytes, is routinely used to identify vessels in tissue sections. Vessel density in tumor specimens, as determined by immuno-histochemical staining for vWF or other endothelial cell markers, is a negative prognostic factor f...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/(sici)1097-0215(20000115)85:2<281::aid-ijc
更新日期:2000-01-15 00:00:00
abstract::Oncogenic mutations of the ras gene leading to constitutive activation of downstream effectors have been detected in a large spectrum of human cancers (pancreas, thyroid, colon and NSCLC). Membrane anchorage of Ras required for functional activity in signal transduction is facilitated by post-translational modificatio...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/(sici)1097-0215(19991008)83:2<283::aid-ijc
更新日期:1999-10-08 00:00:00
abstract::The targeted radiotherapy of neuroblastoma with 131l-labelled metaiodobenzyl guanidine (mIBG) is now the subject of several clinical studies. The precise intracellular localization of mIBG, necessary for nuclear microdosimetry, has not previously been described. We report the use of electron-energy-loss spectroscopy a...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.2910470615
更新日期:1991-04-01 00:00:00
abstract::Creatinine-adjusted levels of estrone, estradiol and estriol were determined in overnight urine specimens from 220 post-menopausal women, aged 54 to 66 years, from Boston (121 women) and Athens (99 women). The associations of individual and total estrogens with various characteristics of the women (age at menopause, y...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.2910400602
更新日期:1987-12-15 00:00:00
abstract::Bone-marrow (BM) hematopoietic precursors are recruited into proliferative activity when colony-stimulating factors (CSF) are sequenced with chemotherapy (CT). Previous studies suggested that further CT can be safely administered only when the increased proliferative activity of these cells has subsided, because most ...
journal_title:International journal of cancer
pub_type: 临床试验,杂志文章
doi:10.1002/ijc.2910630508
更新日期:1995-11-27 00:00:00
abstract::A new murine monoclonal antibody (MAb), E-9, has been raised using tissue-cultured human umbilical vein endothelial cells. The antigen recognized by this MAb is a peptide of 170 kDa under non-reducing conditions and 96 kDa under reducing conditions. MAb E-9 showed marked heterogeneity in its distribution in various ti...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.2910540303
更新日期:1993-05-28 00:00:00
abstract::Our study demonstrates that synthetic sialyl Lewis X (SLex) as a ligand for selectins and fibronectin-derived RGDS peptide analogue [Ar(DRGDS)3] inhibits lung metastases produced by i.v. co-injection of B16-BL6 melanoma cells. To investigate the inhibitory mechanisms in a living animal, we performed positron-emission ...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/(SICI)1097-0215(19960315)65:6<833::AID-IJC
更新日期:1996-03-15 00:00:00
abstract::The aim of this study was to investigate the ability of the new liver organotropic complex of cisplatin with glycocholate (GC), Bamet-R2, to interact with DNA, inhibit its replication and hence reduce tumor-cell proliferation. Changes in the electrophoretic mobility of the open and covalently closed circular forms of ...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/(SICI)1097-0215(19981029)78:3<346::AID-IJC
更新日期:1998-10-29 00:00:00
abstract::Human papillomavirus (HPV) has been identified as a causative agent of cervical cancer and oropharyngeal cancer (OPC). Intriguingly, estrogen and HPV were shown to play synergistic roles in cervical carcinogenesis. We recently demonstrated that the apolipoprotein B mRNA-editing catalytic polypeptide 3 (APOBEC3, A3) fa...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.32500
更新日期:2019-09-15 00:00:00