Abstract:
OBJECTIVE:In association with innate and adaptive immunity, the microbiota controls the colonisation resistance against intestinal pathogens. Caspase recruitment domain 9 (CARD9), a key innate immunity gene, is required to shape a normal gut microbiota. Card9-/- mice are more susceptible to the enteric mouse pathogen Citrobacter rodentium that mimics human infections with enteropathogenic and enterohaemorrhagic Escherichia coli. Here, we examined how CARD9 controls C. rodentium infection susceptibility through microbiota-dependent and microbiota-independent mechanisms. DESIGN:C. rodentium infection was assessed in conventional and germ-free (GF) wild-type (WT) and Card9-/- mice. To explore the impact of Card9-/-microbiota in infection susceptibility, GF WT mice were colonised with WT (WT→GF) or Card9-/- (Card9-/- →GF) microbiota before C. rodentium infection. Microbiota composition was determined by 16S rDNA gene sequencing. Inflammation severity was determined by histology score and lipocalin level. Microbiota-host immune system interactions were assessed by quantitative PCR analysis. RESULTS:CARD9 controls pathogen virulence in a microbiota-independent manner by supporting a specific humoral response. Higher susceptibility to C. rodentium-induced colitis was observed in Card9-/- →GF mice. The microbiota of Card9-/- mice failed to outcompete the monosaccharide-consuming C. rodentium, worsening the infection severity. A polysaccharide-enriched diet counteracted the ecological advantage of C. rodentium and the defective pathogen-specific antibody response in Card9-/- mice. CONCLUSIONS:CARD9 modulates the susceptibility to intestinal infection by controlling the pathogen virulence in a microbiota-dependent and microbiota-independent manner. Genetic susceptibility to intestinal pathogens can be overridden by diet intervention that restores humoural immunity and a competing microbiota.
journal_name
Gutjournal_title
Gutauthors
Lamas B,Michel ML,Waldschmitt N,Pham HP,Zacharioudaki V,Dupraz L,Delacre M,Natividad JM,Costa GD,Planchais J,Sovran B,Bridonneau C,Six A,Langella P,Richard ML,Chamaillard M,Sokol Hdoi
10.1136/gutjnl-2017-314195subject
Has Abstractpub_date
2018-10-01 00:00:00pages
1836-1844issue
10eissn
0017-5749issn
1468-3288pii
gutjnl-2017-314195journal_volume
67pub_type
杂志文章相关文献
GUT文献大全abstract::The effect of a new complex oligosaccharide exhibiting potent inhibitory action on alpha-glucoside hydrolases on intestinal absorption of sucrose in man was tested by constant in vivo perfusion of the jejunum. At concentrations of 4.65 or 15.5 X 10(-6)M the alpha-glucosidehydrolase inhibitor (alpha-GHI) markedly inhib...
journal_title:Gut
pub_type: 杂志文章
doi:10.1136/gut.20.9.750
更新日期:1979-09-01 00:00:00
abstract::The distribution of the endoplasmic reticulum in human hepatocytes is defined in quantitative terms using the techniques of morphometry. The subjects of the study are liver biopsies from normal, untreated subjects and patients being treated with various drugs. In contrast to rat hepatocytes, the amount of smooth endop...
journal_title:Gut
pub_type: 杂志文章
doi:10.1136/gut.15.9.737
更新日期:1974-09-01 00:00:00
abstract:BACKGROUND:Sulfhydryl (SH) compounds are essential in maintaining mucosal integrity in the gastrointestinal tract. A decrease in colonic mucosal SH compounds affects the redox status of the mucosa, resulting in vulnerability to further attacks. Therefore, there is a strong need for in vivo evaluation of SH compounds in...
journal_title:Gut
pub_type: 杂志文章
doi:10.1136/gut.52.9.1291
更新日期:2003-09-01 00:00:00
abstract::Thirty of 81 consecutive HIV antibody positive patients referred with non-cryptosporidial diarrhoea had no potential infectious cause; most had AIDS related complex rather than the full blown syndrome. Opportunistic infections with cytomegalovirus (CMV), mycobacterium avium-intracellulare (MAI), and herpes simplex vir...
journal_title:Gut
pub_type: 杂志文章
doi:10.1136/gut.30.2.195
更新日期:1989-02-01 00:00:00
abstract::This prospective study was undertaken to evaluate the correlation between densitometric values of gall stones assessed by computed tomography and the success rate of litholytic therapy in 28 patients eligible for oral treatment. A densitometric study of the stones was performed in all patients before treatment. A cut ...
journal_title:Gut
pub_type: 杂志文章
doi:10.1136/gut.33.5.698
更新日期:1992-05-01 00:00:00
abstract:OBJECTIVE:Hepatitis D virus (HDV) is a defective virus that completes its life cycle only with hepatitis B virus (HBV). The HBV with HDV super-infection has been considered as one of the most severe forms of the chronic viral hepatitis. However, there is a scarcity of data on the global burden of HDV infection. DESIGN...
journal_title:Gut
pub_type: 杂志文章,meta分析
doi:10.1136/gutjnl-2018-316601
更新日期:2019-03-01 00:00:00
abstract::Family histories were obtained from 50 patients with adenocarcinoma of the colon or rectum in north-east Scotland. A comparable control group was similarly interviewed and in each group the case notes of the first degree relatives were studied where possible. Eight (16%) of the patients with cancer of the colon or rec...
journal_title:Gut
pub_type: 杂志文章
doi:10.1136/gut.23.2.169
更新日期:1982-02-01 00:00:00
abstract:BACKGROUND:Zinc carnosine (ZnC) is a health food product claimed to possess health-promoting and gastrointestinal supportive activity. Scientific evidence underlying these claims is, however, limited. AIM:To examine the effect of ZnC on various models of gut injury and repair, and in a clinical trial. METHODS:In vitr...
journal_title:Gut
pub_type: 杂志文章,随机对照试验
doi:10.1136/gut.2006.099929
更新日期:2007-02-01 00:00:00
abstract::A family is described in which there occurred two cases of the lupoid type of active chronic hepatitis with cirrhosis, one of chronic persistent hepatitis, and one of myasthenia gravis. The two cases of lupoid hepatitis were in the proposita, a schoolgirl aged 16 years, and her great-aunt aged 69 years whom she had ne...
journal_title:Gut
pub_type: 杂志文章
doi:10.1136/gut.11.10.811
更新日期:1970-10-01 00:00:00
abstract::When there is villus atrophy in a jejunal biopsy, intraepithelial lymphocyte (IEL) mitosis correlates with a diagnosis of coeliac disease. We have examined the significance of IEL mitosis in jejunal biopsies with normal villi. Counts of IEL per 100 villus enterocytes, and IEL mitosis per 1000 IEL, were carried out in ...
journal_title:Gut
pub_type: 杂志文章
doi:10.1136/gut.27.6.675
更新日期:1986-06-01 00:00:00
abstract:OBJECTIVE:A high body mass index (BMI) is associated with an increased risk for severe liver disease. It is unclear if this risk differs across BMI categories, and if the association is partially attributed to development of type 2 diabetes mellitus (T2DM). DESIGN:We used register data from more than 1.2 million Swedi...
journal_title:Gut
pub_type: 杂志文章
doi:10.1136/gutjnl-2016-313622
更新日期:2018-08-01 00:00:00
abstract:BACKGROUND:An increasing number of case reports indicate potential nephrotoxicity of 5-aminosalicylic acid (5-ASA), which shares similarities with the chemical structures of both phenacetin and acetylsalicylic acid. AIM:In a point prevalence study the occurrence of sensitive indices indicative of early kidney malfunct...
journal_title:Gut
pub_type: 杂志文章
doi:10.1136/gut.40.6.761
更新日期:1997-06-01 00:00:00
abstract:OBJECTIVE:Gastric cancer (GC) remains difficult to cure due to heterogeneity in a clinical challenge and the molecular mechanisms underlying this disease are complex and not completely understood. Accumulating evidence suggests that microRNAs (miRNAs) play an important role in GC, but the role of specific miRNAs involv...
journal_title:Gut
pub_type: 杂志文章
doi:10.1136/gutjnl-2013-306640
更新日期:2015-02-01 00:00:00
abstract:OBJECTIVE:Bleeding after endoscopic submucosal dissection (ESD) for early gastric cancer (EGC) is a frequent adverse event after ESD. We aimed to develop and externally validate a clinically useful prediction model (BEST-J score: Bleeding after ESD Trend from Japan) for bleeding after ESD for EGC. DESIGN:This retrospe...
journal_title:Gut
pub_type: 杂志文章
doi:10.1136/gutjnl-2019-319926
更新日期:2020-06-04 00:00:00
abstract::Two patients with specific pancreatic amylase deficiency are described. The greatly reduced pancreatic amylase activity was not due to an enzymatically inactive amylase molecule but to an almost complete absence of the molecule itself. The findings are of diagnostic importance as they show that low pancreatic amylase ...
journal_title:Gut
pub_type: 杂志文章
doi:10.1136/gut.32.5.546
更新日期:1991-05-01 00:00:00
abstract::Ultrastructural features of the liver in eight cases of Indian childhood cirrhosis, a fatal and elusive liver disease in India, have been described. Severe parenchymal damage, diffuse creeping fibrosis, and other light microscopic alterations, including the frequent presence of cytoplasmic hyaline, reported earlier ar...
journal_title:Gut
pub_type: 杂志文章
doi:10.1136/gut.12.9.693
更新日期:1971-09-01 00:00:00
abstract::Lower oesophageal sphincter pressures in healthy volunteers were measured by a rapid pull-through technique during intravenous infusion of the histamine H2-receptor antagonist, cimetidine. No consistent effects on sphincter pressure were observed which are liable to be of clinical importance. Serum gastrin concentrati...
journal_title:Gut
pub_type: 杂志文章
doi:10.1136/gut.18.2.99
更新日期:1977-02-01 00:00:00
abstract::Chronic alcohol consumption induces cytochrome P450IIE1, enabling habitual abusers to consume far greater quantities of alcohol than normal subjects. This pathway of metabolism leads to the production of free radical species, which cause tissue damage through peroxidation of cell membranes. Groups of Wistar rats of eq...
journal_title:Gut
pub_type: 杂志文章
doi:10.1136/gut.35.11.1644
更新日期:1994-11-01 00:00:00
abstract::Despite mass population screening and an incidence of EGC in Japan that is at least double that of the West, there seem to be no genuine differences in the clinicopathological features of the disease between the two regions. The macroscopic appearance, size, depth of invasion, frequency of lymph node invasion, and his...
journal_title:Gut
pub_type: 杂志文章,评审
doi:10.1136/gut.41.2.142
更新日期:1997-08-01 00:00:00
abstract::Nineteen healthy subjects were studied and 17 patients with gastric ulcer before and after ulcer healing with carbenoxolone. Gastric deoxytibonucleic acid (DNA) loss was measured as an index of epithelial cell turnover, and N-acetylneuraminic acid (NANA) content of gastric juice as an index of mucus secretion. In norm...
journal_title:Gut
pub_type: 杂志文章
doi:10.1136/gut.18.10.817
更新日期:1977-10-01 00:00:00
abstract:BACKGROUND AND AIMS:Dietary folate is believed to protect against colorectal cancer (CRC). However, few studies have addressed the role of circulating levels of folate. The aim of this study was to relate prediagnostic plasma folate and homocysteine concentrations and the methylenetetrahydrofolate reductase (MTHFR) 677...
journal_title:Gut
pub_type: 杂志文章,多中心研究
doi:10.1136/gut.2005.085480
更新日期:2006-10-01 00:00:00
abstract::Dysphagia in a 79 year old lady was caused by a giant polypoid tumour in mid-oesophagus. Surgery was not appropriate. Shrinkage of the tumour and its eventual detachment were achieved by stopping its blood supply by YAG laser photocoagulation of the tumour stalk. Good, temporary palliation of the dysphagia was achieve...
journal_title:Gut
pub_type: 杂志文章
doi:10.1136/gut.33.8.1146
更新日期:1992-08-01 00:00:00
abstract::Polyacrylamide gel electrophoresis of pure pancreatic juice from 14 healthy normal subjects, 11 chronic alcoholics without detectable pancreatic disease, 15 patients with pancreatitis, and two with cancer of the pancreas consistently demonstrated the presence of two variants of trypsinogen with different electrophoret...
journal_title:Gut
pub_type: 杂志文章
doi:10.1136/gut.20.10.886
更新日期:1979-10-01 00:00:00
abstract:BACKGROUND AND AIMS:Induction of apoptosis of cancer cells through ligands of nuclear hormone receptors (NHRs) is a new approach in cancer therapy. Recently, one of the NHRs, peroxisome proliferator activated receptor gamma (PPARgamma), has been shown to influence cell growth in certain cancer cells although its effect...
journal_title:Gut
pub_type: 杂志文章
doi:10.1136/gut.50.4.563
更新日期:2002-04-01 00:00:00
abstract::The radio-telemetering capsule (radio-pill) has been used to study the return of motility in the small bowel after vagotomy with a drainage procedure, gastric operations not involving vagotomy, and non-gastric operations. Small intestinal movements returned in about 10 hours after vagotomy, about four hours after the ...
journal_title:Gut
pub_type: 杂志文章
doi:10.1136/gut.4.1.77
更新日期:1963-03-01 00:00:00
abstract::Eight patients with diffuse plasma cell infiltration of the small bowel who had the clinical features of immunoproliferative small intestinal disease (IPSID), but whose serum was negative for free alpha-heavy chains, were investigated for evidence of a non-secretory form of alpha-chain disease (alpha-CD). Molecular si...
journal_title:Gut
pub_type: 杂志文章
doi:10.1136/gut.26.9.928
更新日期:1985-09-01 00:00:00
abstract::Levels of clotting factors II, V, and VII were measured on admission and then daily in 12 patients with grade IV hepatic coma due to fulminant hepatic failure. Factor VII levels obtained within 36 hours of the development of grade IV coma were not of value in predicting which patients would subsequently recover consci...
journal_title:Gut
pub_type: 杂志文章
doi:10.1136/gut.17.7.489
更新日期:1976-07-01 00:00:00
abstract::In 18 patients intermediary anabolic metabolites of 5-FU were measured in normal colonic mucosa and colorectal cancer tissue of intravenous bolus injection or continuous infusion. Higher total concentrations of 5-FU products were found in the cancers when compared with normal colonic tissue. There appeared to be no ev...
journal_title:Gut
pub_type: 杂志文章
doi:10.1136/gut.27.2.176
更新日期:1986-02-01 00:00:00
abstract::The absorption of short chain fatty acids by the human rectum in the absence of chloride has been studied using a dialysis bag technique. Absorption rates of sodium, water, and short chain fatty acids were unchanged on replacing chloride with sulphate. Final concentrations of bicarbonate were 35.4 +/- 3.4 mmol/l in th...
journal_title:Gut
pub_type: 杂志文章
doi:10.1136/gut.20.5.400
更新日期:1979-05-01 00:00:00
abstract::The effect of increasing the calcium, magnesium and sodium concentration in gall bladder bile samples from 21 patients with gall stones and nine controls on the in vitro rate of formation of cholesterol microcrystals and numbers of cholesterol microcrystals formed was examined. Addition of these cations to raise the m...
journal_title:Gut
pub_type: 杂志文章
doi:10.1136/gut.30.5.665
更新日期:1989-05-01 00:00:00