Abstract:
:Present-day terrestrial analogue sites are crucial ground truth proxies for studying life in geochemical conditions close to those assumed to be present on early Earth or inferred to exist on other celestial bodies (e.g. Mars, Europa). Although hypersaline sapropels are border-of-life habitats with moderate occurrence, their microbiological and physicochemical characterization lags behind. Here, we study the diversity of life under low water activity by describing the prokaryotic communities from two disparate hypersaline sapropels (Transylvanian Basin, Romania) in relation to geochemical milieu and pore water chemistry, while inferring their role in carbon cycling by matching taxa to known taxon-specific biogeochemical functions. The polyphasic approach combined deep coverage SSU rRNA gene amplicon sequencing and bioinformatics with RT-qPCR and physicochemical investigations. We found that sapropels developed an analogous elemental milieu and harbored prokaryotes affiliated with fifty-nine phyla, among which the most abundant were Proteobacteria, Bacteroidetes and Chloroflexi. Containing thirty-two candidate divisions and possibly undocumented prokaryotic lineages, the hypersaline sapropels were found to accommodate one of the most diverse and novel ecosystems reported to date and may contribute to completing the phylogenetic branching of the tree of life.
journal_name
Sci Repjournal_title
Scientific reportsauthors
Andrei AŞ,Baricz A,Robeson MS 2nd,Păuşan MR,Tămaş T,Chiriac C,Szekeres E,Barbu-Tudoran L,Levei EA,Coman C,Podar M,Banciu HLdoi
10.1038/s41598-017-06232-wsubject
Has Abstractpub_date
2017-07-21 00:00:00pages
6150issue
1issn
2045-2322pii
10.1038/s41598-017-06232-wjournal_volume
7pub_type
杂志文章abstract::Plant defensins (PDFs) are cysteine-rich peptides that have a range of biological functions, including defence against fungal pathogens. However, little is known about their role in defence against bacteria. In this study, we showed that the protein encoded by ARABIDOPSIS THALIANA PLANT DEFENSIN TYPE 1.1 (AtPDF1.1) is...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-017-08497-7
更新日期:2017-08-23 00:00:00
abstract::Most women with ovarian cancer are diagnosed at an advanced stage and there are few therapeutic options. Recently, monoclonal antibody therapies have had limited success, thus more effective antibodies are needed to improve long-term survival. In this report, we prepared polyclonal rabbit anti-ovarian cancer antibody ...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep04984
更新日期:2014-05-15 00:00:00
abstract::High levels of reproductive failure were detected in some Spanish sow farms in the Spring of 2010. Regular returns to estrus and variable reductions in litter size were observed. The problem started suddenly and did not appear to be related to the quality of the ejaculates, disease, alterations of body condition or an...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep04913
更新日期:2014-05-09 00:00:00
abstract::Measurements of volcanic tephra fallout deposits provide useful information about the magnitude and intensity of explosive volcanic eruptions and potential for remobilization of deposits as dangerous volcanic flows. However, gathering information in the vicinity of erupting craters is extremely dangerous, and moreover...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-71902-1
更新日期:2020-09-09 00:00:00
abstract::Neuropsychological assessment of human visual processing capabilities strongly depends on visual testing conditions including room lighting, stimuli, and viewing-distance. This limits standardization, threatens reliability, and prevents the assessment of core visual functions such as visual processing speed. Increasin...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep37016
更新日期:2016-11-21 00:00:00
abstract::Monanchoxymycalin C (MomC) is a new marine pentacyclic guanidine alkaloid, recently isolated from marine sponge Monanchora pulchra by us. Here, anticancer activity and mechanism of action was investigated for the first time using a human prostate cancer (PCa) model. MomC was active in all PCa cell lines at low micromo...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-69751-z
更新日期:2020-08-06 00:00:00
abstract::Employing theranostic nanoparticles, which combine both therapeutic and diagnostic capabilities in one dose, has promise to propel the biomedical field toward personalized medicine. Here we investigate the theranostic properties of topological insulator bismuth selenide (Bi2Se3) in in vivo and in vitro system for the ...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep01998
更新日期:2013-01-01 00:00:00
abstract::Salt crystallization is a major cause of weathering of rocks, artworks and monuments. Damage can only occur if crystals continue to grow in confinement, i.e. within the pore space of these materials, thus generating mechanical stress. We report the direct measurement, at the microscale, of the force exerted by growing...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep30856
更新日期:2016-08-05 00:00:00
abstract::We study the spreading of cell aggregates deposited on adhesive substrates decorated with microparticles (MPs). A cell monolayer expands around the aggregate. The cells on the periphery of the monolayer take up the MPs, clearing the substrate as they progress and forming an aureole of cells filled with MPs. We study t...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-017-15665-2
更新日期:2017-11-16 00:00:00
abstract::RNA interference (RNAi) is a robust tool to study gene functions as well as potential for insect pest control. Finding suitable target genes is the key step in the development of an efficient RNAi-mediated pest control technique. Based on the transcriptome of Chilo suppressalis, 24 unigenes which putatively associated...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep28697
更新日期:2016-06-29 00:00:00
abstract::Short flashes of blue light (bioluminescence) from dinoflagellates can reduce copepod grazing of light-emitting cells. Other protective strategies against grazing are toxicity, reduced cell chain length and altered swimming patterns in different phytoplankton. Both toxicity and bioluminescence capacity in dinoflagella...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-017-13293-4
更新日期:2017-10-12 00:00:00
abstract::Microorganisms are key components for plant biomass breakdown within rumen environments. Fibrobacter succinogenes have been identified as being active and dominant cellulolytic members of the rumen. In this study, F. succinogenes type strain S85 was adapted for steady state growth in continuous culture at pH 5.75 and ...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-017-02628-w
更新日期:2017-05-23 00:00:00
abstract::Water Droplet Erosion (WDE) as a material degradation phenomenon has been a concern in power generation industries for decades. Steam turbine blades and the compressor blades of gas turbines that use water injection usually suffer from WDE. The present work focuses on studying erosion resistance of TiAl as a potential...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep14182
更新日期:2015-09-22 00:00:00
abstract::Lactobacilli are thought to be beneficial for human health, with lactobacilli-associated infections being confined to immune-compromised individuals. However, Lactobacillus fermentum AGR1487 negatively affects barrier integrity in vitro so we hypothesized that it caused a pro-inflammatory response in the host. We comp...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep20318
更新日期:2016-02-04 00:00:00
abstract::The stinging capsules of cnidarians, nematocysts, function as harpoon-like organelles with unusual biomechanical properties. The nanosecond discharge of the nematocyst requires a dense protein network of the capsule structure withstanding an internal pressure of up to 150 bar. Main components of the capsule are short ...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep25709
更新日期:2016-05-11 00:00:00
abstract::Probing the oligomeric state of abundant molecules, such as membrane proteins in intact cells, is essential, but has not been straightforward. We address this challenge with a simple counting strategy that is capable of reporting the oligomeric state of dense, membrane-bound protein complexes. It is based on single-mo...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep14072
更新日期:2015-09-11 00:00:00
abstract::Resistance to epidermal growth factor receptor-tyrosine kinase inhibitors (EGFR-TKIs) has become the main clinical challenge of advanced lung cancer. This research aimed to explore the role of PARP1-mediated autophagy in the progression of TKI therapy. PARP1-mediated autophagy was evaluated in vitro by CCK-8 assay, cl...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-77908-z
更新日期:2020-12-01 00:00:00
abstract::The hippocampus has a significant association with memory, cognition and emotions. The dopaminergic projections from both the ventral tegmental area and substantia nigra are thought to be involved in hippocampal activity. To date, however, few studies have investigated dopaminergic innervation in the hippocampus or th...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep44426
更新日期:2017-03-14 00:00:00
abstract::Baroreflex dysfunction is a diffuse chronic condition that is expected to be followed by a profound loss of organization of BP and HR variability. Nevertheless, long-term effects of baroreflex withdrawal are still debated. Aim of our work was to study BP and HR changes long term after sino-aortic denervation (SAD). In...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-018-37970-0
更新日期:2019-02-04 00:00:00
abstract::We propose a method to accelerate small-angle scattering experiments by exploiting spatial correlation in two-dimensional data. We applied kernel density estimation to the average of a hundred short scans and evaluated noise reduction effects of kernel density estimation (smoothing). Although there is no advantage of ...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-018-37345-5
更新日期:2019-02-06 00:00:00
abstract::Muscular dystrophy-dystroglycanopathies comprise a heterogeneous and complex group of disorders caused by loss-of-function mutations in a multitude of genes that disrupt the glycobiology of α-dystroglycan, thereby affecting its ability to function as a receptor for extracellular matrix proteins. Of the various genes i...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-019-46431-1
更新日期:2019-07-11 00:00:00
abstract::gH625 constitutes a promising delivery vehicle for the transport of therapeutic biomacromolecules across membrane barriers. We report an application of multivalency to create a complex nanosystem for delivery and to elucidate the mechanism of peptide-lipid bilayer interactions. Multivalency may offer a route to enhanc...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-017-09001-x
更新日期:2017-08-25 00:00:00
abstract::Dogs (Canis familiaris) are the first animals to be domesticated by humans and the only ones domesticated by mobile hunter-gatherers. Wolves and humans were both persistent, pack hunters of large prey. They were species competing over resources in partially overlapping ecological niches and capable of killing each oth...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-78214-4
更新日期:2021-01-07 00:00:00
abstract::Sequence-controlled polymers (SCPs) such as DNA and proteins play an important role in biology. Many efforts have been devoted to synthesize SCPs in the past half a century. However, to our knowledge, the artificial sequences containing independently functional groups have never been reported. Here, we present a facil...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep04387
更新日期:2014-03-17 00:00:00
abstract::Mitochondria play critical roles in meeting cellular energy demand, in cell death, and in reactive oxygen species (ROS) and stress signaling. Most Caenorhabditis elegans loss-of-function (lf) mutants in nuclear-encoded components of the respiratory chain are non-viable, emphasizing the importance of respiratory functi...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep29695
更新日期:2016-07-21 00:00:00
abstract::Evolutionary processes, speciation in woody bamboos are presently little understood. Here we used Dendrocalamus sinicus Chia & J.L. Sun as a model species to investigate dispersal or vicariance speciation in woody bamboos. Variation in three chloroplast DNA (cpDNA) fragments and eight simple sequence repeat markers (S...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-018-35269-8
更新日期:2018-11-16 00:00:00
abstract::Epidemiological studies have shown that atherosclerotic risk factors accelerate the pathological process underlying Alzheimer's disease (AD) via chronic cerebral hypoperfusion. In this study, we aimed to clarify the mechanisms by which cerebral hypoperfusion may exacerbate AD pathology. We applied bilateral common car...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-019-39494-7
更新日期:2019-02-26 00:00:00
abstract::The role of marginal populations for the long-term maintenance of species' genetic diversity and evolutionary potential is particularly timely in view of the range shifts caused by climate change. The Centre-Periphery hypothesis predicts that marginal populations should bear reduced genetic diversity and have low evol...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep42416
更新日期:2017-02-10 00:00:00
abstract::This corrects the article DOI: 10.1038/srep20067. ...
journal_title:Scientific reports
pub_type: 杂志文章,已发布勘误
doi:10.1038/srep46387
更新日期:2017-06-30 00:00:00
abstract::Tissue macrophage-derived apoptosis inhibitor of macrophage (AIM, encoded by cd5l gene) is a circulating protein that has suppressive functions in a broad range of diseases including obesity, liver steatosis, hepatocellular carcinoma (HCC), and acute kidney injury (AKI). In healthy states, high levels of AIM circulate...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-018-31580-6
更新日期:2018-09-03 00:00:00