Abstract:
:Mitochondria play critical roles in meeting cellular energy demand, in cell death, and in reactive oxygen species (ROS) and stress signaling. Most Caenorhabditis elegans loss-of-function (lf) mutants in nuclear-encoded components of the respiratory chain are non-viable, emphasizing the importance of respiratory function. Chromophore-Assisted Light Inactivation (CALI) using genetically-encoded photosensitizers provides an opportunity to determine how individual respiratory chain components contribute to physiology following acute lf. As proof-of-concept, we expressed the 'singlet oxygen generator' miniSOG as a fusion with the SDHC subunit of respiratory complex II, encoded by mev-1 in C. elegans, using Mos1-mediated Single Copy Insertion. The resulting mev-1::miniSOG transgene complemented mev-1 mutant phenotypes in kn1 missense and tm1081(lf) deletion mutants. Complex II activity was inactivated by blue light in mitochondria from strains expressing active miniSOG fusions, but not those from inactive fusions. Moreover, light-inducible phenotypes in vivo demonstrated that complex II activity is important under conditions of high energy demand, and that specific cell types are uniquely susceptible to loss of complex II. In conclusion, miniSOG-mediated CALI is a novel genetic platform for acute inactivation of respiratory chain components. Spatio-temporally controlled ROS generation will expand our understanding of how the respiratory chain and mitochondrial ROS influence whole organism physiology.
journal_name
Sci Repjournal_title
Scientific reportsauthors
Wojtovich AP,Wei AY,Sherman TA,Foster TH,Nehrke Kdoi
10.1038/srep29695subject
Has Abstractpub_date
2016-07-21 00:00:00pages
29695issn
2045-2322pii
srep29695journal_volume
6pub_type
杂志文章abstract::Epitope characterization is critical for elucidating the mechanism of action of drug candidates. However, traditional high-resolution epitope mapping techniques are not well suited for screening numerous drug candidates recognizing a similar target. Here, we use Hydrogen-Deuterium Exchange Mass Spectrometry (HDX-MS) t...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-019-41179-0
更新日期:2019-03-18 00:00:00
abstract::Adolescence is a critical stage of development and has an important influence on energy balance-related behaviours (EBRBs). When adolescents are associated with obesity it can lead to increased cardiometabolic risk. Here we assess if EBRBs adopted by adolescents included in a subsample are associated with markers of t...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-019-42859-7
更新日期:2019-04-23 00:00:00
abstract::Spin torque oscillator (STO) has been attracting a great deal of attention as a candidate for the next generation microwave signal sources for various modern electronics systems since its advent. However, the phase noise of STOs under free running oscillation is still too large to be used in practical microwave applic...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep18134
更新日期:2015-12-11 00:00:00
abstract::Three main surveillance systems (laboratory-confirmed, influenza-like illness (ILI) and nationwide Notifiable Infectious Diseases Reporting Information System (NIDRIS)) have been used for influenza surveillance in China. However, it is unclear which surveillance system is more reliable in developing influenza early wa...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-018-21059-9
更新日期:2018-02-12 00:00:00
abstract::The GacS/GacA two-component system (also called GrrS/GrrA) is a global regulatory system which is highly conserved among gamma-proteobacteria. This system positively regulates non-coding small regulatory RNA csrB, which in turn binds to the RNA-binding protein CsrA. However, how GacS/GacA-Csr system regulates virulenc...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep37195
更新日期:2016-11-15 00:00:00
abstract::Systemic lipopolysaccharide (LPS) is implicated in increasing mortality in patients with alcoholic hepatitis but the underlying mechanisms are not well characterised. The objective of this study was to characterise neutrophil function, LPS and cytokine concentrations within the splanchnic circulation of alcoholic cirr...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep40157
更新日期:2017-01-04 00:00:00
abstract::Low-noise millimetre-wave signals are valuable for digital sampling systems, arbitrary waveform generation for ultra-wideband communications, and coherent radar systems. However, the phase noise of widely used conventional signal generators (SGs) will increase as the millimetre-wave frequency increases. Our goal has b...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep24621
更新日期:2016-05-17 00:00:00
abstract::In large random networks, each eigenvector of the Laplacian matrix tends to localize on a subset of network nodes having similar numbers of edges, namely, the components of each Laplacian eigenvector take relatively large values only on a particular subset of nodes whose degrees are close. Although this localization p...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-017-01010-0
更新日期:2017-04-25 00:00:00
abstract::A tribochemistry-assisted method has been developed for nondestructive surface nanofabrication on GaAs. Without any applied electric field and post etching, hollow nanostructures can be directly fabricated on GaAs surfaces by sliding a SiO2 microsphere under an ultralow contact pressure in humid air. TEM observation o...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep09020
更新日期:2015-03-12 00:00:00
abstract::Dyslipidemia in pregnancy are associated with risk of adverse outcomes. As an adverse pregnancy outcome, small-for-gestational-age has been extensively studied in Western countries. However, similar studies have rarely been conducted in Asian countries. Data were derived from 5695 pairs of non-diabetic mothers and neo...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-76845-1
更新日期:2020-11-16 00:00:00
abstract::An abrupt onset of the new tongue-shaped deformation of magnetic surface in magnetized plasmas, which was conjectured in since the 1960s but has not been observed, is experimentally identified just before an abrupt onset of a large-scale collapse event. Two novel properties of the event are identified. First, the tran...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep36217
更新日期:2016-10-31 00:00:00
abstract::A novel imaging-driven technique with an integrated fluorescence signature to enable automated enumeration of two species of cyanobacteria and an alga of somewhat similar morphology to one of the cyanobacteria is presented to demonstrate proof-of-concept that high accuracy, imaging-based, rapid water quality analysis ...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-018-27406-0
更新日期:2018-06-13 00:00:00
abstract::Toll-like receptors (TLRs) play a key role in innate and adaptive immunity, protecting the host from viral pathogens. We studied the effect of TLR7 polymorphisms on disease susceptibility and progression of chronic hepatitis B (CHB) infection in Chinese adults. Blood samples were taken from 612 patients with confirmed...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-017-12698-5
更新日期:2017-09-29 00:00:00
abstract::Spinal Muscular Atrophy (SMA) is caused by mutation or deletion of the survival motor neuron 1 (SMN1) gene. Decreased levels of, cell-ubiquitous, SMN protein is associated with a range of systemic pathologies reported in severe patients. Despite high levels of SMN protein in normal liver, there is no comprehensive stu...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep34635
更新日期:2016-10-04 00:00:00
abstract::Dendritic spines are small, bulbous protrusions along dendrites in neurons and play a critical role in synaptic transmission. Dendritic spines come in a variety of shapes that depend on their developmental state. Additionally, roughly 14-19% of mature spines have a specialized endoplasmic reticulum called the spine ap...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-019-48028-0
更新日期:2019-08-12 00:00:00
abstract::The catalytic and optical properties of metal nanoparticles can be combined to create platforms for light-driven chemical energy storage and enhanced in-situ reaction monitoring. However, the heavily damped plasmon resonances of many catalytically active metals (e.g. Pt, Pd) prevent this dual functionality in pure nan...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep17431
更新日期:2015-11-30 00:00:00
abstract::Empirical yield estimation from satellite data has long lacked suitable combinations of spatial and temporal resolutions. Consequently, the selection of metrics, i.e., temporal descriptors that predict grain yield, has likely been driven by practicality and data availability rather than by systematic targetting of cri...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-019-51715-7
更新日期:2019-10-31 00:00:00
abstract::Primary IgA nephropathy (IgAN) diagnosis is based on IgA-dominant glomerular deposits and histological scoring is done on formalin-fixed paraffin embedded tissue (FFPE) sections using the Oxford classification. Our aim was to use this underexploited resource to extract RNA and identify genes that characterize active (...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-72026-2
更新日期:2020-09-16 00:00:00
abstract::Motivational, cognitive and action goals are processed by distinct, topographically organized, corticostriatal circuits. We aimed to test whether processing in the striatum is under causal control by cortical regions in the human brain by investigating the effects of offline transcranial magnetic stimulation (TMS) ove...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-018-21346-5
更新日期:2018-02-19 00:00:00
abstract::We investigated the level of amyloid beta (Aβ) in nasal secretions of patients with Alzheimer's disease dementia (ADD) using interdigitated microelectrode (IME) biosensors and determined the predictive value of Aβ in nasal secretions for ADD diagnosis. Nasal secretions were obtained from 35 patients with ADD, 18 with ...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-019-41429-1
更新日期:2019-03-21 00:00:00
abstract::To explore the importance of soil microbial community composition on explaining the difference in heterotrophic soil respiration (R(h)) across forests, a field investigation was conducted on Rh and soil physiochemical and microbial properties in four subtropical forests in southern China. We observed that Rh differed ...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep07854
更新日期:2015-01-19 00:00:00
abstract::Polymers have appealing optical, biochemical, and mechanical qualities, including broadband transparency, ease of functionalization, and biocompatibility. However, their low refractive indices have precluded wavelength-scale optical confinement and nanophotonic applications in polymers. Here, we introduce a suspended ...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep02145
更新日期:2013-01-01 00:00:00
abstract::In the primary somatosensory cortex, large-scale cortical and perceptual changes have been demonstrated following input deprivation. Recently, we found that the cortical and perceptual changes induced by repetitive somatosensory stimulation (RSS) at a finger transfer to the face. However, whether such cross-border cha...
journal_title:Scientific reports
pub_type: 临床试验,杂志文章
doi:10.1038/s41598-018-30183-5
更新日期:2018-08-06 00:00:00
abstract::We report a novel method to fabricate high quality 2-inch freestanding GaN substrate grown on cross-stacked carbon nanotubes (CSCNTs) coated sapphire by hydride vapor phase epitaxy (HVPE). As nanoscale masks, these CSCNTs can help weaken the interface connection and release the compressive stress by forming voids duri...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep28620
更新日期:2016-06-24 00:00:00
abstract::Retinal diseases are frequently characterized by the accumulation of excessive scar tissue found throughout the neural retina. However, the pathophysiology of retinal fibrosis remains poorly understood, and the cell types that contribute to the fibrotic response are incompletely defined. Here, we show that myofibrobla...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-72875-x
更新日期:2020-09-25 00:00:00
abstract::The optical conversion of incoherent solar radiation into a bright, coherent laser beam enables the application of nonlinear optics to solar energy conversion and storage. Here, we present an architecture for solar pumped lasers that uses a luminescent solar concentrator to decouple the conventional trade-off between ...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep14758
更新日期:2015-10-05 00:00:00
abstract::Indoor simulation experiment was carried out to evaluate the formation and consumption rates of methane (CH4) in Xiangxi Bay of the Three Gorges Reservoir (TGR), China. The results show that both the CH4 formation and consumption rates were significantly positively correlated with temperature. CH4 efflux decreased wit...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep04449
更新日期:2014-03-24 00:00:00
abstract::We have successfully grown centimeter-sized layered [Formula: see text] single crystals under high oxygen pressures of 120-150 bar by the floating zone technique. This enabled us to perform neutron scattering experiments where we observe close to quarter-integer magnetic peaks below [Formula: see text] that are accomp...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-74884-2
更新日期:2020-10-22 00:00:00
abstract::The black rhinoceros is again on the verge of extinction due to unsustainable poaching in its native range. Despite a wide historic distribution, the black rhinoceros was traditionally thought of as depauperate in genetic variation, and with very little known about its evolutionary history. This knowledge gap has hamp...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep41417
更新日期:2017-02-08 00:00:00
abstract::Bisphenol A (BPA) is one of the commonest Endocrine Disruptor Compounds worldwide. It interferes with vertebrate reproduction, possibly by inducing deregulation of epigenetic mechanisms. To determine its effects on female reproductive physiology and investigate whether changes in the expression levels of genes related...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep21982
更新日期:2016-02-25 00:00:00