Abstract：
:The stinging capsules of cnidarians, nematocysts, function as harpoon-like organelles with unusual biomechanical properties. The nanosecond discharge of the nematocyst requires a dense protein network of the capsule structure withstanding an internal pressure of up to 150 bar. Main components of the capsule are short collagens, so-called minicollagens, that form extended polymers by disulfide reshuffling of their cysteine-rich domains (CRDs). Although CRDs have identical cysteine patterns, they exhibit different structures and disulfide connectivity at minicollagen N and C-termini. We show that the structurally divergent CRDs have different cross-linking potentials in vitro and in vivo. While the C-CRD can participate in several simultaneous intermolecular disulfides and functions as a cystine knot after minicollagen synthesis, the N-CRD is monovalent. Our combined experimental and computational analyses reveal the cysteines in the C-CRD fold to exhibit a higher structural propensity for disulfide bonding and a faster kinetics of polymerization. During nematocyst maturation, the highly reactive C-CRD is instrumental in efficient cross-linking of minicollagens to form pressure resistant capsules. The higher ratio of C-CRD folding types evidenced in the medusozoan lineage might have fostered the evolution of novel, predatory nematocyst types in cnidarians with a free-swimming medusa stage.
journal_name
Sci Repjournal_title
Scientific reportsauthors
Tursch A,Mercadante D,Tennigkeit J,Gräter F,Özbek Sdoi
10.1038/srep25709subject
Has Abstractpub_date
2016-05-11 00:00:00pages
25709issn
2045-2322pii
srep25709journal_volume
6pub_type
杂志文章abstract：:Coffee contains caffeine and diterpenes that were associated with decreased breast cancer risk, but results remained inconsistent. The study purpose was to investigate the associations between coffee products and breast cancer risk among Hong Kong Chinese women. We conducted a hospital-based case-control study in thre...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-49205-x
更新日期：2019-09-03 00:00:00
abstract：:Underutilization due to performance limitations imposed by species and charge transports is one of the key issues that persist with various lithium-ion batteries. To elucidate the relevant mechanisms, two groups of characteristic parameters were proposed. The first group contains three characteristic time parameters, ...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep32639
更新日期：2016-09-07 00:00:00
abstract：:Antibiotics contamination in food products is of increasing concern due to their potential threat on human health. Herein solid-phase extraction based on magnetic molecularly imprinted polymers coupled with internal extractive electrospray ionization mass spectrometry (MMIPs-SPE-iEESI-MS) was designed for the quantita...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-017-15202-1
更新日期：2017-11-07 00:00:00
abstract：:Accurate recording of large, earthquake-induced ground shaking is critical for our understanding of earthquake physics as well as seismic hazard assessment. Extremely large accelerations with the peak value of 3.2 times the gravity acceleration were recorded at seismic station WTMC located in northern South Island of ...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-018-37716-y
更新日期：2019-02-04 00:00:00
abstract：:Pepper (Capsicum annuum L.) fruit is sensitive to temperatures below 10 °C, which severely diminish fruit quality during cold chain distribution. Seed browning was a major chilling symptom in 36 genotypes of C. annuum fruit screened after storage at 2 °C for 3 weeks. Among them, pepper fruits of chilling-insensitive '...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-75055-z
更新日期：2020-10-21 00:00:00
abstract：:MicroRNAs (miRNAs) are important post-transcriptional regulators involved in animal adipogenesis, however, their roles in bovine fat deposition remain poorly understood. In the present study, we conducted a comparative RNA sequencing to identify the key miRNAs involved in beef lipid accumulation by comparing the backf...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep44026
更新日期：2017-03-08 00:00:00
abstract：:It is well recognized that osteocytes communicate with each other via gap junctions and that connxin43 (Cx43) shows its great potential in gap junction for the contribution enabling transmission of small molecules and operating in an autocrine/a paracrine manner. Fibroblast growth factors (FGFs) play significant roles...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-018-33247-8
更新日期：2018-10-04 00:00:00
abstract：:Phosphoinositides play important roles in numerous intracellular membrane pathways. Little is known about the regulation or function of these lipids in rod photoreceptor cells, which have highly active membrane dynamics. Using new assays with femtomole sensitivity, we determined that whereas levels of phosphatidylinos...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep26978
更新日期：2016-06-01 00:00:00
abstract：:Trichosporon asahii is a pathogenic fungus that causes deep mycosis in patients with neutropenia. Establishing an experimental animal model for quantitatively evaluating pathogenicity and developing a genetic recombination technology will help to elucidate the infection mechanism of T. asahii and promote the developme...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-67841-6
更新日期：2020-07-03 00:00:00
abstract：:Establishing robust genome engineering methods in the malarial parasite, Plasmodium falciparum, has the potential to substantially improve the efficiency with which we gain understanding of this pathogen's biology to propel treatment and elimination efforts. Methods for manipulating gene expression and engineering the...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-77644-4
更新日期：2021-01-11 00:00:00
abstract：:Ferroelectric tunneling junctions (FTJs) with tunable tunneling electroresistance (TER) are promising for many emerging applications, including non-volatile memories and neurosynaptic computing. One of the key challenges in FTJs is the balance between the polarization value and the tunneling current. In order to achie...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-56816-x
更新日期：2019-12-31 00:00:00
abstract：:Soybean cyst nematode (SCN) Heterodera glycines is an obligate parasite that relies on the secretion of effector proteins to manipulate host cellular processes that favor the formation of a feeding site within host roots to ensure its survival. The sequence complexity and co-evolutionary forces acting upon these effec...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-018-20536-5
更新日期：2018-02-06 00:00:00
abstract：:Insects are extremely successful animals whose odor perception is very prominent due to their sophisticated olfactory system. The main chemosensory organ, antennae play a critical role in detecting odor in ambient environment before initiating appropriate behavioral responses. The antennal chemosensory receptor genes ...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep24078
更新日期：2016-04-19 00:00:00
abstract：:Cyanobacteria are a diverse group of photosynthetic bacteria that exhibit phototaxis, or motion in response to light. Cyanobacteria such as Synechocystis sp. secrete a mixture of complex polysaccharides that facilitate cell motion, while their type 4 pili allow them to physically attach to each other. Even though cell...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-017-18160-w
更新日期：2017-12-19 00:00:00
abstract：:Accurately representing the Indian Ocean Dipole (IOD) is crucial for reliable climate predictions and future projections. However, El Niño-Southern Oscillation (ENSO) and IOD interact, making it necessary to evaluate ENSO and IOD simultaneously. Using the historical simulation from 32 fifth phase of Coupled Model Inte...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-68268-9
更新日期：2020-07-13 00:00:00
abstract：:The objective of this study was to develop markedly improved risk prediction models for lung cancer using a prospective cohort of 395,875 participants in Taiwan. Discriminatory accuracy was measured by generation of receiver operator curves and estimation of area under the curve (AUC). In multivariate Cox regression a...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep36482
更新日期：2016-11-02 00:00:00
abstract：:A bound state in the continuum (BIC) is an unusual localized state that is embedded in a continuum of extended states. Here, we present the general condition for BICs to arise from wave equation separability. Then we show that by exploiting perturbations of certain symmetry such BICs can be turned into resonances that...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep33394
更新日期：2016-09-19 00:00:00
abstract：:Wetting phenomena in multi-phase systems govern the shape of the contact line which separates the different phases. For liquids in contact with solid surfaces wetting is typically described in terms of contact angle. While in macroscopic systems the contact angle can be determined experimentally, on the molecular scal...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep26111
更新日期：2016-05-17 00:00:00
abstract：:Prostate cancer is the most commonly diagnosed cancer in men with African American men disproportionally suffering from the burden of this disease. Biomarkers that could discriminate indolent from aggressive and drug resistance disease are lacking. MicroRNAs are small non-coding RNAs that affect numerous physiological...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-46170-3
更新日期：2019-07-05 00:00:00
abstract：:In face-to-face communication, audio-visual (AV) stimuli can be fused, combined or perceived as mismatching. While the left superior temporal sulcus (STS) is presumably the locus of AV integration, the process leading to combination is unknown. Based on previous modelling work, we hypothesize that combination results ...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-75201-7
更新日期：2020-10-22 00:00:00
abstract：:For isotropic fluids, classical nucleation theory predicts the nucleation rate, barrier height and critical droplet size by ac- counting for the competition between bulk energy and interfacial tension. The nucleation process in liquid crystals is less understood. We numerically investigate nucleation in monolayered ne...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep40059
更新日期：2017-01-05 00:00:00
abstract：:The cation diffusion facilitator (CDF) is a conserved family of divalent d-block metal cation transporters that extrude these cations selectively from the cytoplasm. CDF proteins are composed of two domains: the transmembrane domain, through which the cations are transported, and a regulatory cytoplasmic C-terminal do...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-71036-4
更新日期：2020-08-20 00:00:00
abstract：:We employed an RNA-guided CRISPR/Cas9 DNA editing system to precisely remove the entire HIV-1 genome spanning between 5' and 3' LTRs of integrated HIV-1 proviral DNA copies from latently infected human CD4+ T-cells. Comprehensive assessment of whole-genome sequencing of HIV-1 eradicated cells ruled out any off-target ...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep22555
更新日期：2016-03-04 00:00:00
abstract：:Since 2001, hundreds of thousands of hours of underwater acoustic recordings have been made throughout the Southern Ocean south of 60° S. Detailed analysis of the occurrence of marine mammal sounds in these circumpolar recordings could provide novel insights into their ecology, but manual inspection of the entirety of...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-78995-8
更新日期：2021-01-12 00:00:00
abstract：:A Quality Improvement bundle was implemented with the goal of standardizing the multidisciplinary approach to delivery room management. We used a Pre-Post Quality Improvement initiative with the following aims: (1) Placement of a functioning pulse oximeter by two minutes after birth, (2) Delayed intubation, (3) Normot...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep37397
更新日期：2016-11-21 00:00:00
abstract：:Effects of remote ischemic conditioning (RIC) in acute myocardial infarction (AMI) patients remain conflicting. We performed this meta-analysis of randomized clinical trials (RCTs) to evaluate the benefits of the RIC in patients with AMI. Potentially relevant RCTs were identified by searching PubMed, Embase, Cochrane ...
journal_title：Scientific reports
pub_type： 杂志文章,meta分析
doi：10.1038/srep43529
更新日期：2017-03-08 00:00:00
abstract：:Cucumber is an agriculturally and economically important vegetable crop worldwide. Fruit flesh thickness is an important trait for cucumber and also a central determinant of yield, yet little is known about the underlying mechanism of this trait. In this study, bulked segregant analysis (BSA) combined with specific le...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep15829
更新日期：2015-10-28 00:00:00
abstract：:We report on an all-dielectric, polarization-independent angular filter with one-dimension (1D) photonic crystal (PC) composed of semiconductor compatible Si/SiO2 pairs. The near-symmetric directional band gap of p- and s-polarized components and Fabry-Pérot (F-P) resonances are utilized to realize efficient polarizat...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-017-16837-w
更新日期：2017-11-29 00:00:00
abstract：:Si holds great promise as an alloying anode material for Li-ion batteries with improved energy density because of its high theoretical specific capacity and favorable operation voltage range. However, the large volume expansion of Si during electrochemical reaction with Li and the associated adverse effects strongly l...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-017-13261-y
更新日期：2017-10-12 00:00:00
abstract：:To explore the pathologies of Kashin-Beck disease (KBD) and KBD accompanied with dental fluorosis (DF), we conducted a comparative analysis of gene expression profiles. 12 subjects were recruited, including 4 KBD patients, 4 patients with KBD and DF and 4 healthy subjects. Genome-wide expression profiles from their pe...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-017-18519-z
更新日期：2018-01-09 00:00:00
