Abstract：
:Establishing robust genome engineering methods in the malarial parasite, Plasmodium falciparum, has the potential to substantially improve the efficiency with which we gain understanding of this pathogen's biology to propel treatment and elimination efforts. Methods for manipulating gene expression and engineering the P. falciparum genome have been validated. However, a significant barrier to fully leveraging these advances is the difficulty associated with assembling the extremely high AT content DNA constructs required for modifying the P. falciparum genome. These are frequently unstable in commonly-used circular plasmids. We address this bottleneck by devising a DNA assembly framework leveraging the improved reliability with which large AT-rich regions can be efficiently manipulated in linear plasmids. This framework integrates several key functional genetics outcomes via CRISPR/Cas9 and other methods from a common, validated framework. Overall, this molecular toolkit enables P. falciparum genetics broadly and facilitates deeper interrogation of parasite genes involved in diverse biological processes.
journal_name
Sci Repjournal_title
Scientific reportsauthors
Nasamu AS,Falla A,Pasaje CFA,Wall BA,Wagner JC,Ganesan SM,Goldfless SJ,Niles JCdoi
10.1038/s41598-020-77644-4subject
Has Abstractpub_date
2021-01-11 00:00:00pages
342issue
1issn
2045-2322pii
10.1038/s41598-020-77644-4journal_volume
11pub_type
杂志文章abstract：:Cytomegalovirus (CMV) is one of the infectious causes of hypertensive anterior uveitis, which is characterized by recurrent episodes of elevated intraocular pressure (IOP) and mild anterior uveitis. Despite the potentially vision-threatening complications of this disease, the underlying mechanisms remain largely undef...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep43349
更新日期：2017-02-27 00:00:00
abstract：:Several prenatal and perinatal factors have been found to be associated with developmental dyslexia (reading disorder) in alphabetic language. Given the absence of relevant studies of Chinese children, the present study tries to investigate these risk factors. A total of 45,850 students were recruited from grades thre...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep36697
更新日期：2016-11-07 00:00:00
abstract：:The systematics of Oriental freshwater mussels (Bivalvia: Unionidae) is poorly known. Here, we present an integrative revision of the genus Trapezoideus Simpson, 1900 to further understanding of freshwater mussel diversity in the region. We demonstrate that Trapezoideus as currently circumscribed is non-monophyletic, ...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-39365-1
更新日期：2019-03-11 00:00:00
abstract：:In utero and upon delivery, neonates are exposed to a wide array of microorganisms from various sources, including maternal bacteria. Prior studies have proposed that the mode of feeding shapes the gut microbiota and, subsequently the child's health. However, the effect of the mode of feeding and its influence on the ...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep38309
更新日期：2016-12-06 00:00:00
abstract：:Cystatin C (CysC) is implicated in neuroprotection and repair in the nervous system in response to diverse neurotoxic conditions. In addition to being secreted from cells in a soluble form, CysC is released by cells in association with extracellular vesicles (EVs), including exosomes. We demonstrate that EVs containin...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-47524-7
更新日期：2019-07-31 00:00:00
abstract：:Highly malignant triple-negative breast cancer (TNBC) cells rely mostly on glycolysis to maintain cellular homeostasis; however, mitochondria are still required for migration and metastasis. Taking advantage of the metabolic flexibility of TNBC MDA-MB-231 cells to generate subpopulations with glycolytic or oxidative p...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-018-31367-9
更新日期：2018-09-04 00:00:00
abstract：:Silica columns from PCR purification and gel extraction kits are widely used in laboratories worldwide to assist in gene cloning. However, the use of these columns can generate plastic waste that has an environmental impact due to their one-off design and massive consumption. Thus, it is important to develop a novel m...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-018-30316-w
更新日期：2018-08-27 00:00:00
abstract：:Dorso-ventral (DV) countershading is a highly-conserved pigmentary adaptation in vertebrates. In mammals, spatially regulated expression of agouti-signaling protein (ASIP) generates the difference in shading by driving a switch between the production of chemically-distinct melanins in melanocytes in dorsal and ventral...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-40251-z
更新日期：2019-03-05 00:00:00
abstract：:Novel natural products 7R, 8R, 7'R, 9'S-verniciasin A (1a), 7S, 8S, 7'S, 9'R- verniciasin A (1b), 7R, 8R, 7'R, 9'S-7'-methoxylverniciasin A (2a) and 7S, 8S, 7'S, 9'R-7'-methoxylverniciasin A (2b) were characterized from the seed capsule of Vernicia fordii. And the unique 9-O-9'-7, 9'-cyclo-8, 1'-neolignan skeleton wit...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-017-14062-z
更新日期：2017-10-19 00:00:00
abstract：:Covert facial mimicry involves subtle facial muscle activation in observers when they perceive the facial emotional expressions of others. It remains uncertain whether prototypical facial features in emotional expressions are being covertly mimicked and also whether covert facial mimicry involves distinct facial muscl...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-61563-5
更新日期：2020-03-13 00:00:00
abstract：:Animal morphology and behavior often appear to evolve cooperatively. However, it is difficult to assess how strictly these two traits depend on each other. The genitalia morphologies and courtship behaviors in insects, which vary widely, may be a good model for addressing this issue. In Diptera, phylogenetic analyses ...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-018-36301-7
更新日期：2019-01-18 00:00:00
abstract：:To develop and externally validate a prostate health index (PHI)-based nomogram for predicting the presence of prostate cancer (PCa) at biopsy in Chinese men with prostate-specific antigen 4-10 ng/mL and normal digital rectal examination (DRE). 347 men were recruited from two hospitals between 2012 and 2014 to develop...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep15341
更新日期：2015-10-16 00:00:00
abstract：:Virtual planning of open cranial vault reconstruction is used to simulate and define an pre-operative plan for craniosynostosis surgery. However, virtual planning techniques are subjective and dependent on the experience and preferences of the surgical team. To develop an objective automated 3D pre-operative planning ...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-018-21662-w
更新日期：2018-02-20 00:00:00
abstract：:Silicon nanocrystals offer huge advantages compared to other semi-conductor quantum dots as they are made from an abundant, non-toxic material and are compatible with silicon devices. Besides, among a wealth of extraordinary properties ranging from catalysis to nanomedicine, metal nanoparticles are known to increase t...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep02672
更新日期：2013-01-01 00:00:00
abstract：:Water is the most important plasticizer of biological and organic hydrophilic materials, which generally exhibit enhanced mechanical softness and molecular mobility upon hydration. The enhancement of the molecular dynamics upon mixing with water, which in glass-forming systems implies a lower glass transition temperat...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-017-07643-5
更新日期：2017-08-07 00:00:00
abstract：:The subtle mechanisms by which protein-DNA interactions remain functional across a wide range of temperatures are largely unknown. In this work, we manually curated available information relating fully sequenced archaeal genomes with organism growth temperatures. We built a motif that represents the core promoter of e...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-018-19495-8
更新日期：2018-01-22 00:00:00
abstract：:Pulse generation often requires a stabilized cavity and its corresponding mode structure for initial phase-locking. Contrastingly, modeless cavity-free random lasers provide new possibilities for high quantum efficiency lasing that could potentially be widely tunable spectrally and temporally. Pulse generation in rand...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep18526
更新日期：2015-12-21 00:00:00
abstract：:Identifying the occurrence mechanism of drug-induced side effects (SEs) is critical for design of drug target and new drug development. The expression of genes in biological processes is regulated by transcription factors(TFs) and/or microRNAs. Most of previous studies were focused on a single level of gene or gene se...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-017-06083-5
更新日期：2017-07-20 00:00:00
abstract：:In recent decades Madagascar has experienced significant habitat loss and modification, with minimal understanding of how human land use practices have impacted the evolution of its flora and fauna. In light of ongoing and intensifying anthropogenic pressures, we seek new insight into mechanisms driving genetic variab...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-52689-2
更新日期：2019-11-07 00:00:00
abstract：:Double-strand breaks (DSBs) are the most lethal DNA damages induced by ionising radiation (IR) and their efficient repair is crucial to limit genomic instability. The cellular DSB response after low IR doses is of particular interest but its examination requires the analysis of high cell numbers. Here, we present an a...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-018-35660-5
更新日期：2018-11-23 00:00:00
abstract：:The IκB kinase (IKK) complex acts as the gatekeeper of canonical NF-κB signaling, thereby regulating immunity, inflammation and cancer. It consists of the catalytic subunits IKKα and IKKβ and the regulatory subunit NEMO/IKKγ. Here, we show that the ubiquitin binding domain (UBAN) in NEMO is essential for IKK/NF-κB act...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep18934
更新日期：2016-01-07 00:00:00
abstract：:Maternal obesity and overnutrition during pregnancy and lactation can program an increased risk of obesity in offspring. In this context, improving maternal metabolism may help reduce the intergenerational transmission of obesity. Here we show that, in Sprague-Dawley rats, selectively altering obese maternal gut micro...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep20683
更新日期：2016-02-12 00:00:00
abstract：:Chronic myeloid leukemia (CML) is characterized in the majority of cases by a t(9;22)(q34;q11) translocation, also called the Philadelphia chromosome, giving rise to the BCR-ABL1 fusion protein. Current treatment with tyrosine kinase inhibitors is directed against the constitutively active ABL1 domain of the fusion pr...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-017-00755-y
更新日期：2017-04-04 00:00:00
abstract：:Feedback information is essential for us to adapt appropriately to the environment. The feedback-related negativity (FRN), a frontocentral negative deflection after the delivery of feedback, has been found to be larger for outcomes that are worse than expected, and it reflects a reward prediction error derived from th...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep34032
更新日期：2016-10-03 00:00:00
abstract：:We study the spreading of cell aggregates deposited on adhesive substrates decorated with microparticles (MPs). A cell monolayer expands around the aggregate. The cells on the periphery of the monolayer take up the MPs, clearing the substrate as they progress and forming an aureole of cells filled with MPs. We study t...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-017-15665-2
更新日期：2017-11-16 00:00:00
abstract：:Sediment accretion (burial) and nutrient enrichment occur concurrently in lacustrine wetlands, but the role of these two aspects of sedimentation on macrophyte performance has rarely been examined. Here, we investigated the concurrent effects of sediment accretion and nutrient enrichment on the growth and propagation ...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep39963
更新日期：2017-01-05 00:00:00
abstract：:Prenatal stress is known to epigenetically program offspring physiology and behaviour, and may become a risk factor for adult complex diseases. To gain insight into the underlying environment-gene interactions, we used proton nuclear magnetic resonance spectroscopy to analyze urinary metabolomes of male and female ado...
journal_title：Scientific reports
pub_type： 临床试验,杂志文章
doi：10.1038/s41598-018-31230-x
更新日期：2018-08-28 00:00:00
abstract：:Highly Tm(3+) doped optical fibers are urgently desirable for 2.0 μm compact single-frequency fiber laser and high-repetition-rate mode-locked fiber laser. Here, we systematically investigated the optical parameters, energy transfer processes and thermal properties of Tm(3+) doped barium gallo-germanate (BGG) glasses....
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep20344
更新日期：2016-02-01 00:00:00
abstract：:Clustered regularly interspaced short palindromic repeats (CRISPR) in conjunction with CRISPR-associated proteins (Cas) can be employed to introduce double stand breaks into mammalian genomes at user-defined loci. The endonuclease activity of the Cas complex can be targeted to a specific genomic region using a single ...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep14987
更新日期：2015-10-08 00:00:00
abstract：:We study the tight-binding model for a graphene tube with perimeter N threaded by a magnetic field. We show exactly that this model has different nontrivial topological phases as the flux changes. The winding number, as an indicator of topological quantum phase transition (QPT) fixes at N/3 if N/3 equals to its intege...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep31953
更新日期：2016-08-24 00:00:00
