Abstract：
:The cation diffusion facilitator (CDF) is a conserved family of divalent d-block metal cation transporters that extrude these cations selectively from the cytoplasm. CDF proteins are composed of two domains: the transmembrane domain, through which the cations are transported, and a regulatory cytoplasmic C-terminal domain (CTD). It was recently shown that the CTD of the CDF protein MamM from magnetotactic bacteria has a role in metal selectivity, as binding of different metal cations exhibits distinctive affinities and conformations. It is yet unclear whether the composition of the CTD binding sites can impact metal selectivity and if we can manipulate the CTD to response to other non-native metals in CDF proteins. Here we performed a mutational study of the model protein MamM CTD, where we exchanged the native metal binding residues with different metal-binding amino acids. Using X-ray crystallography and Trp-fluorescence spectrometry, we studied the impact of these mutations on the CTD conformation in the presence of non-native metals. Our results reveal that the incorporation of such mutations alters the domain response to metals in vitro, as mutant forms of the CTD bind metals differently in terms of the composition of the binding sites and the CTD conformation. Therefore, the results demonstrate the direct influence of the CTD binding site composition on CDF proteins structure and hence, function, and constitute a first step for rational design of MamM for transporting different metals in vivo.
journal_name
Sci Repjournal_title
Scientific reportsauthors
Barber-Zucker S,Shahar A,Kolusheva S,Zarivach Rdoi
10.1038/s41598-020-71036-4subject
Has Abstractpub_date
2020-08-20 00:00:00pages
14022issue
1issn
2045-2322pii
10.1038/s41598-020-71036-4journal_volume
10pub_type
杂志文章abstract：:In this work we study theoretically and experimentally the multi-particle structure of the so-called type-II quantum dots with spatially separated electrons and holes. Our calculations based on customarily developed full configuration interaction ap- proach reveal that exciton complexes containing holes interacting wi...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep45568
更新日期：2017-03-30 00:00:00
abstract：:Infectious diseases outbreaks are often characterized by a spatial component induced by hosts' distribution, mobility, and interactions. Spatial models that incorporate hosts' movements are being used to describe these processes, to investigate the conditions for propagation, and to predict the spatial spread. Several...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep00476
更新日期：2012-01-01 00:00:00
abstract：:The dispersion of organic N-benzyl-2-methyl-4-nitroaniline (BNA) in nematic liquid crystals (LCs) is studied. BNA doping decreases the threshold voltage of cell because of the reduced splay elastic constant and increased dielectric anisotropy of the LC mixture. When operated in the high voltage difference condition, t...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-71306-1
更新日期：2020-08-31 00:00:00
abstract：:Nanostructure based color filtering has been considered an attractive replacement for current colorant pigmentation in the display technologies, in view of its increased efficiencies, ease of fabrication and eco-friendliness. For such structural filtering, iridescence relevant to its angular dependency, which poses a ...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep04921
更新日期：2014-05-12 00:00:00
abstract：:Novel tools in humane animal research should benefit the animal as well as the experimentally obtained data. Imaging technologies have proven to be versatile and also in accordance with the demands of the 3 R principle. However, most imaging technologies are either limited by the target organs, number of repetitive im...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-65950-w
更新日期：2020-06-08 00:00:00
abstract：:The long-standing challenge for forming Al-based BMGs and their matrix composites with a critical size larger than 1 mm have not been answered over the past three decades. In this paper, we reported formation of a series of BMG matrix composites which contain a high Al content up to 55 at.%. These composites can be ca...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep03353
更新日期：2013-11-27 00:00:00
abstract：:Identification of low-abundance, low-molecular-weight native peptides using non-tryptic plasma has long remained an unmet challenge, leaving potential bioactive/biomarker peptides undiscovered. We have succeeded in efficiently removing high-abundance plasma proteins to enrich and comprehensively identify low-molecular...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-79353-4
更新日期：2021-01-13 00:00:00
abstract：:The structure of bonds in biomolecules, such as base pairs in RNA chains or native interactions in proteins, can be presented in the form of a chord diagram. A given biomolecule is then characterized by the genus of an auxiliary two-dimensional surface associated to such a diagram. In this work we introduce the notion...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-018-35557-3
更新日期：2018-12-03 00:00:00
abstract：:To characterize the molecular mechanisms underlying life-stage transitions in Phytophthora infestans, we initiated a chemical genetics approach by screening for a stage-specific inhibitor of morphological development from microbial culture extracts prepared mostly from actinomycetes from soil in Japan. Of the more tha...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-79326-7
更新日期：2020-12-18 00:00:00
abstract：:Vascular leakage is a life-threatening complication of dengue virus (DENV) infection. Previously, association between "paracellular" endothelial hyperpermeability and plasma leakage had been extensively investigated. However, whether "transcellular" endothelial leakage is involved in dengue hemorrhagic fever (DHF) and...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep31855
更新日期：2016-08-22 00:00:00
abstract：:Sacrospinous ligament fixation (SSLF) is one of the most utilized surgeries in the management of pelvic organ prolapse (POP). We conducted a large-series study of SSLF in a tertiary center by an experienced urogynecologic team. The 453 women with POP who underwent SSLF at National Taiwan University Hospital in the per...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-61995-z
更新日期：2020-03-19 00:00:00
abstract：:The phylogenetic diversity of marine bacteria belonged to the Bacillus cereus group has not been well investigated. Here, we present the genetic diversity and population structure of 71 bacteria from diverse marine environments, using a multilocus sequence typing (MLST) approach and the analyses of digital DNA-DNA hyb...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-017-00817-1
更新日期：2017-04-06 00:00:00
abstract：:Nano-flowers reported so far were mostly constituted of inorganic or hybrid materials. We have synthesized and crystallized a new organic compound, 1, 2-bis(tritylthio)ethane forming an organic nano-flower consisting of single crystalline petals. Crystal structure at nano and micro level indicates that π-π stacking in...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-017-17538-0
更新日期：2017-12-11 00:00:00
abstract：:The characterization of network community structure has profound implications in several scientific areas. Therefore, testing the algorithms developed to establish the optimal division of a network into communities is a fundamental problem in the field. We performed here a highly detailed evaluation of community detec...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep02216
更新日期：2013-01-01 00:00:00
abstract：:Prescribing the right drug with the right dose is a central tenet of precision medicine. We examined the use of patients' prior Electronic Health Records to predict a reduction in drug dosage. We focus on drugs that interact with the P450 enzyme family, because their dosage is known to be sensitive and variable. We ex...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-018-33980-0
更新日期：2018-10-22 00:00:00
abstract：:A facile carbothermal route was adopted to obtain niobium carbide nanoparticles (NPs) embedded in carbon network from Nb2O5 to study photocatalytic behavior. Optimization of synthesis parameters to obtain single phase NbC NPs has been successfully done. The phase identification, morphology and nature of carbon were de...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-018-31989-z
更新日期：2018-09-11 00:00:00
abstract：:Metamaterial absorbers can provide good solutions for radar-cross-section (RCS) reduction. In spite of their attractive features of thinness, lightness, and low cost, resonant metamaterial absorbers have a drawback of narrow bandwidth. For practical radar applications, wideband absorbers are necessary. In this paper, ...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep31823
更新日期：2016-08-22 00:00:00
abstract：:In the last decades, AC powered atmospheric dielectric barrier discharges (DBDs) in air with a liquid electrode have been proposed as a promising plasma technology with versatile applicability in medicine, agriculture and water treatment. The fundamental features of the micro-discharge filaments that make up this type...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-018-29189-w
更新日期：2018-07-19 00:00:00
abstract：:The underlying reasons behind the unprecedented increase of the mortality rates due to the opioid epidemics in the United States are still not fully uncovered. Most efforts have been focused on targeting opioids, but there is little information about vulnerable populations at high risk of opioid abuse and death. In th...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-61281-y
更新日期：2020-03-09 00:00:00
abstract：:SOX7 as a tumor suppressor belongs to the SOX F gene subfamily and is associated with a variety of human cancers, including breast cancer, but the mechanisms involved are largely unclear. In the current study, we investigated the interactions between SOX7 and AXIN2 in their co-regulation on the Wnt/β-catenin signal pa...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep26136
更新日期：2016-05-18 00:00:00
abstract：:Huntington's disease (HD) is a devastating hereditary movement disorder, characterized by degeneration of neurons in the striatum and cortex. Studies in human patients and mouse HD models suggest that disturbances of neuronal function in the neocortex play an important role in disease onset and progression. However, t...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-43024-w
更新日期：2019-04-29 00:00:00
abstract：:Non-human animals sometimes show marked intraspecific variation in their cognitive abilities that may reflect variation in external inputs and experience during the developmental period. We examined variation in exploration and cognitive performance on a problem-solving task in a large sample of captive orang-utans (P...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep40052
更新日期：2017-01-09 00:00:00
abstract：:The importance of self-feedback autaptic transmission in modulating spike-time irregularity is still poorly understood. By using a biophysical model that incorporates autaptic coupling, we here show that self-innervation of neurons participates in the modulation of irregular neuronal firing, primarily by regulating th...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep26096
更新日期：2016-05-17 00:00:00
abstract：:Nowadays, various collaborations are creating immense machines to try to track and understand the origin of high-energy cosmic particles (e.g., IceCube, ANTARES, Baikal-GVD, P-ONE). The detection mechanism of these sophisticated experiments relies mainly on an optical signal generated by the passage of charged particl...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-68954-8
更新日期：2020-07-24 00:00:00
abstract：:African swine fever virus (ASFV) is a highly pathogenic, double-stranded DNA virus with a marked tropism for cells of the monocyte-macrophage lineage, affecting swine species and provoking severe economic losses and health threats. In the present study, four established porcine cell lines, IPAM-WT, IPAM-CD163, C∆2+ an...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-017-09948-x
更新日期：2017-09-04 00:00:00
abstract：:Some insect galls are formed on sumac plants by certain aphid species and have been used for medicinal and chemical purposes as they are rich in tannins. The most prominent species among gall aphids in China is Schlechtendalia chinensis, which formed horn-shaped galls on the winged rachis of Rhus chinensis. S. chinens...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-017-06806-8
更新日期：2017-07-25 00:00:00
abstract：:Amplification of liver injury is mediated by macrophages but the signaling by which the macrophage inflammasome enhances liver injury is not completely understood. The CCAAT/Enhancer Binding Protein-β (C/EBPβ) is a critical signaling molecule for macrophages because expression of a dominant inhibitor of C/EBPβ DNA-bin...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep24268
更新日期：2016-04-12 00:00:00
abstract：:While implicated in therapeutic resistance, malignant progenitor cell cycle kinetics have been difficult to quantify in real-time. We developed an efficient lentiviral bicistronic fluorescent, ubiquitination-based cell cycle indicator reporter (Fucci2BL) to image live single progenitors on a defined niche coupled with...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep23885
更新日期：2016-04-04 00:00:00
abstract：:Kohlschutter-Tönz syndrome (KTS) is a rare genetic disorder with neurological dysfunctions including seizure and intellectual impairment. Mutations at the Rogdi locus have been linked to development of KTS, yet the underlying mechanisms remain elusive. Here we demonstrate that a Drosophila homolog of Rogdi acts as a n...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-017-11941-3
更新日期：2017-09-12 00:00:00
abstract：:Primates are now known to possess well-developed olfactory sensitivity and discrimination capacities that can play a substantial role in many aspects of their interaction with conspecifics and the environment. Several studies have demonstrated that olfactory cues may be useful in fruit selection. Here, using a conditi...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep14895
更新日期：2015-10-06 00:00:00
