Tracking of Normal and Malignant Progenitor Cell Cycle Transit in a Defined Niche.

Abstract:

:While implicated in therapeutic resistance, malignant progenitor cell cycle kinetics have been difficult to quantify in real-time. We developed an efficient lentiviral bicistronic fluorescent, ubiquitination-based cell cycle indicator reporter (Fucci2BL) to image live single progenitors on a defined niche coupled with cell cycle gene expression analysis. We have identified key differences in cell cycle regulatory gene expression and transit times between normal and chronic myeloid leukemia progenitors that may inform cancer stem cell eradication strategies.

journal_name

Sci Rep

journal_title

Scientific reports

authors

Pineda G,Lennon KM,Delos Santos NP,Lambert-Fliszar F,Riso GL,Lazzari E,Marra MA,Morris S,Sakaue-Sawano A,Miyawaki A,Jamieson CH

doi

10.1038/srep23885

subject

Has Abstract

pub_date

2016-04-04 00:00:00

pages

23885

issn

2045-2322

pii

srep23885

journal_volume

6

pub_type

杂志文章
  • Moderate drop in water table increases peatland vulnerability to post-fire regime shift.

    abstract::Northern and tropical peatlands represent a globally significant carbon reserve accumulated over thousands of years of waterlogged conditions. It is unclear whether moderate drying predicted for northern peatlands will stimulate burning and carbon losses as has occurred in their smaller tropical counterparts where the...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/srep08063

    authors: Kettridge N,Turetsky MR,Sherwood JH,Thompson DK,Miller CA,Benscoter BW,Flannigan MD,Wotton BM,Waddington JM

    更新日期:2015-01-27 00:00:00

  • Compact multilayer film structure for angle insensitive color filtering.

    abstract::Here we report a compact multilayer film structure for angle robust color filtering, which is verified by theoretical calculations and experiment results. The introduction of the amorphous silicon in the proposed unsymmetrical resonant cavity greatly reduces the angular sensitivity of the filters, which is confirmed b...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/srep09285

    authors: Yang C,Shen W,Zhang Y,Li K,Fang X,Zhang X,Liu X

    更新日期:2015-03-19 00:00:00

  • NADPH oxidase 4 is required for the generation of macrophage migration inhibitory factor and host defense against Toxoplasma gondii infection.

    abstract::Nicotinamide adenine dinucleotide phosphate (NADPH) oxidases (Nox) are an important family of catalytic enzymes that generate reactive oxygen species (ROS), which mediate the regulation of diverse cellular functions. Although phagocyte Nox2/gp91phox is closely associated with the activation of host innate immune respo...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-017-06610-4

    authors: Kim JH,Lee J,Bae SJ,Kim Y,Park BJ,Choi JW,Kwon J,Cha GH,Yoo HJ,Jo EK,Bae YS,Lee YH,Yuk JM

    更新日期:2017-07-25 00:00:00

  • Full-length cloning, sequence analysis and expression detection of the β-tubulin gene from the Chinese gall aphid (Schlechtendalia chinensis).

    abstract::Some insect galls are formed on sumac plants by certain aphid species and have been used for medicinal and chemical purposes as they are rich in tannins. The most prominent species among gall aphids in China is Schlechtendalia chinensis, which formed horn-shaped galls on the winged rachis of Rhus chinensis. S. chinens...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-017-06806-8

    authors: Liu P,Yang ZX,Chen XM,Chen H

    更新日期:2017-07-25 00:00:00

  • Regulatory role of G9a and LSD1 in the Transcription of Olfactory Receptors during Leukaemia Cell Differentiation.

    abstract::Recent studies have reported the ectopic expression of olfactory receptors (ORs) in non-olfactory tissues, however, their physiological roles were not well elucidated. ORs are expressed in and function in different types of cancers. Here, we identified that the H3K9me2 levels of several OR promoters decreased during d...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/srep46182

    authors: Jung H,Chae YC,Kim JY,Jeong OS,Kook H,Seo SB

    更新日期:2017-04-07 00:00:00

  • Less is more: Avoiding the LIBS dimensionality curse through judicious feature selection for explosive detection.

    abstract::Despite its intrinsic advantages, translation of laser induced breakdown spectroscopy for material identification has been often impeded by the lack of robustness of developed classification models, often due to the presence of spurious correlations. While a number of classifiers exhibiting high discriminatory power h...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/srep13169

    authors: Kumar Myakalwar A,Spegazzini N,Zhang C,Kumar Anubham S,Dasari RR,Barman I,Kumar Gundawar M

    更新日期:2015-08-19 00:00:00

  • Molecular and functional characterization of the BMPR2 gene in Pulmonary Arterial Hypertension.

    abstract::Pulmonary arterial hypertension is a progressive disease that causes the obstruction of precapillary pulmonary arteries and a sustained increase in pulmonary vascular resistance. The aim was to analyze functionally the variants found in the BMPR2 gene and to establish a genotype-phenotype correlation. mRNA expression ...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-017-02074-8

    authors: Pousada G,Lupo V,Cástro-Sánchez S,Álvarez-Satta M,Sánchez-Monteagudo A,Baloira A,Espinós C,Valverde D

    更新日期:2017-05-15 00:00:00

  • Differentiation of malignant cervical lymphadenopathy by dual-energy CT: a preliminary analysis.

    abstract::The accurate diagnosis of malignant cervical lymphadenopathy remains challenging. In this study, we determined the value of quantitative parameters derived from dual-energy computed tomography (DECT) for differentiating malignant cervical lymphadenopathy caused by thyroid carcinoma (TC), salivary gland carcinoma (SC),...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/srep31020

    authors: Yang L,Luo D,Li L,Zhao Y,Lin M,Guo W,Zhou C

    更新日期:2016-08-08 00:00:00

  • Herbal formula YYJD inhibits tumor growth by inducing cell cycle arrest and senescence in lung cancer.

    abstract::Lung cancer represents a major cause of cancer-related death worldwide. Although various tactics and anti-tumor drugs have been used to improve curative effects, five-year survival rate of lung cancer patients remains poor. In this study, we investigated the action and underlying mechanisms of our recently optimized C...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-017-05146-x

    authors: Zheng T,Que Z,Jiao L,Kang Y,Gong Y,Yao J,Ma C,Bi L,Dong Q,Zhao X,Xu L

    更新日期:2017-07-10 00:00:00

  • Retinal Pigment Epithelium Cell Density and Bruch's Membrane Thickness in Secondary versus Primary High Myopia and Emmetropia.

    abstract::To assess differences between secondary high myopia (SHM) due to congenital glaucoma and primary high myopia (PHM) and non-highly myopic eyes (NHM) in the relationships between axial length and Bruch's membrane (BM) thickness and retinal pigment epithelium (RPE) density. The histomorphometric study included human glob...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-020-62096-7

    authors: Jonas JB,Li D,Holbach L,Panda-Jonas S

    更新日期:2020-03-20 00:00:00

  • IgE-mediated enhancement of CD4(+) T cell responses requires antigen presentation by CD8α(-) conventional dendritic cells.

    abstract::IgE, forming an immune complex with small proteins, can enhance the specific antibody and CD4(+) T cell responses in vivo. The effects require the presence of CD23 (Fcε-receptor II)(+) B cells, which capture IgE-complexed antigens (Ag) in the circulation and transport them to splenic B cell follicles. In addition, als...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/srep28290

    authors: Ding Z,Dahlin JS,Xu H,Heyman B

    更新日期:2016-06-16 00:00:00

  • The effect of anxiety on emotional recognition: evidence from an ERP study.

    abstract::Anxiety-related bias in the recognition memory based on trait anxiety has induced some studies. Their results, however, were conflicting. In fact, anxious differences not only differed from personality traits but also from different anxiety mood levels. We explored the emotional memory bias in both trait and state anx...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-018-34289-8

    authors: Yu Q,Zhuang Q,Wang B,Liu X,Zhao G,Zhang M

    更新日期:2018-11-01 00:00:00

  • Floating mechanism of a small liquid marble.

    abstract::Flotation of small solid objects and liquid droplets on water is critical to natural and industrial activities. This paper reports the floating mechanism of liquid marbles, or liquid droplets coated with hydrophobic microparticles. We used X-ray computed tomography (XCT) to acquire cross-sectional images of the floati...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/srep21777

    authors: Ooi CH,Plackowski C,Nguyen AV,Vadivelu RK,St John JA,Dao DV,Nguyen NT

    更新日期:2016-02-23 00:00:00

  • DNA hypomethylation upregulates expression of the MGAT3 gene in HepG2 cells and leads to changes in N-glycosylation of secreted glycoproteins.

    abstract::Changes in N-glycosylation of plasma proteins are observed in many types of cancer, nevertheless, few studies suggest the exact mechanism involved in aberrant protein glycosylation. Here we studied the impact of DNA methylation on the N-glycome in the secretome of the HepG2 cell line derived from hepatocellular carcin...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/srep24363

    authors: Klasić M,Krištić J,Korać P,Horvat T,Markulin D,Vojta A,Reiding KR,Wuhrer M,Lauc G,Zoldoš V

    更新日期:2016-04-13 00:00:00

  • Deletion of biosynthetic genes, specific SNP patterns and differences in transcript accumulation cause variation in hydroxynitrile glucoside content in barley cultivars.

    abstract::Barley (Hordeum vulgare L.) produces five leucine-derived hydroxynitrile glucosides, potentially involved in alleviating pathogen and environmental stresses. These compounds include the cyanogenic glucoside epiheterodendrin. The biosynthetic genes are clustered. Total hydroxynitrile glucoside contents were previously ...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-019-41884-w

    authors: Ehlert M,Jagd LM,Braumann I,Dockter C,Crocoll C,Motawia MS,Møller BL,Lyngkjær MF

    更新日期:2019-04-05 00:00:00

  • MnGa-based fully perpendicular magnetic tunnel junctions with ultrathin Co2MnSi interlayers.

    abstract::Because tetragonal structured MnGa alloy has intrinsic (not interface induced) giant perpendicular magnetic anisotropy (PMA), ultra-low damping constant and high spin polarization, it is predicted to be a kind of suitable magnetic electrode candidate in the perpendicular magnetic tunnel junction (p-MTJ) for high densi...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/srep43064

    authors: Mao S,Lu J,Zhao X,Wang X,Wei D,Liu J,Xia J,Zhao J

    更新日期:2017-02-24 00:00:00

  • Implicit and explicit systems differently predict possible dangers.

    abstract::One strategy to address new potential dangers is to generate defensive responses to stimuli that remind learned threats, a phenomenon called fear generalization. During a threatening experience, the brain encodes implicit and explicit memory traces. Nevertheless, there is a lack of studies comparing implicit and expli...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-019-49751-4

    authors: Manassero E,Mana L,Concina G,Renna A,Sacchetti B

    更新日期:2019-09-16 00:00:00

  • Comparative mode of action of the antimicrobial peptide melimine and its derivative Mel4 against Pseudomonas aeruginosa.

    abstract::Melimine and Mel4 are chimeric cationic peptides with broad-spectrum antimicrobial activity. They have been shown to be highly biocompatible in animal models and human clinical trials. The current study examined the mechanism of action of these two antimicrobial peptides against P. aeruginosa. The effect of the peptid...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-019-42440-2

    authors: Yasir M,Dutta D,Willcox MDP

    更新日期:2019-05-08 00:00:00

  • Microbial biotransformation of DON: molecular basis for reduced toxicity.

    abstract::Bacteria are able to de-epoxidize or epimerize deoxynivalenol (DON), a mycotoxin, to deepoxy-deoxynivalenol (deepoxy-DON or DOM-1) or 3-epi-deoxynivalenol (3-epi-DON), respectively. Using different approaches, the intestinal toxicity of 3 molecules was compared and the molecular basis for the reduced toxicity investig...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/srep29105

    authors: Pierron A,Mimoun S,Murate LS,Loiseau N,Lippi Y,Bracarense AP,Schatzmayr G,He JW,Zhou T,Moll WD,Oswald IP

    更新日期:2016-07-06 00:00:00

  • Effects of plant species richness on 13C assimilate partitioning in artificial grasslands of different established ages.

    abstract::Artificial grasslands play a role in carbon storage on the Qinghai-Tibetan Plateau. The artificial grasslands exhibit decreased proportions of graminate and increased species richness with age. However, the effect of the graminate proportions and species richness on ecosystem C stocks in artificial grasslands have not...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/srep40307

    authors: Xu L,Yao B,Wang W,Wang F,Zhou H,Shi J,Zhao X

    更新日期:2017-01-09 00:00:00

  • Seasonal induction of alternative principal pathway for rose flower scent.

    abstract::Ecological adaptations to seasonal changes are often observed in the phenotypic traits of plants and animals, and these adaptations are usually expressed through the production of different biochemical end products. In this study, ecological adaptations are observed in a biochemical pathway without alteration of the e...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/srep20234

    authors: Hirata H,Ohnishi T,Tomida K,Ishida H,Kanda M,Sakai M,Yoshimura J,Suzuki H,Ishikawa T,Dohra H,Watanabe N

    更新日期:2016-02-01 00:00:00

  • Selective binding of facial features reveals dynamic expression fragments.

    abstract::The temporal correspondence between two arbitrarily chosen pairs of alternating features can generally be reported for rates up to 3-4 Hz. This limit is however surpassed for specialised visual mechanisms that encode conjunctions of features. Here we show that this 3-4 Hz limit is exceeded for eye gaze and eyebrow pai...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-018-27242-2

    authors: Harrison C,Binetti N,Mareschal I,Johnston A

    更新日期:2018-06-13 00:00:00

  • Tailoring thermal conductivity via three-dimensional porous alumina.

    abstract::Three-dimensional anodic alumina templates (3D-AAO) are an astonishing framework with open highly ordered three-dimensional skeleton structures. Since these templates are architecturally different from conventional solids or porous templates, they teem with opportunities for engineering thermal properties. By establis...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/srep38595

    authors: Abad B,Maiz J,Ruiz-Clavijo A,Caballero-Calero O,Martin-Gonzalez M

    更新日期:2016-12-09 00:00:00

  • Isolation and comparative genomics of Mycobacterium tuberculosis isolates from cattle and their attendants in South India.

    abstract::The major human pathogen Mycobacterium tuberculosis is rarely reported to cause disease in other animals. Cases in livestock are thought to occur through contact with infected handlers, but previous studies evaluating putative livestock-human transmission used typing techniques with limited resolution. Here, we undert...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-019-54268-x

    authors: Palaniyandi K,Kumar N,Veerasamy M,Kabir Refaya A,Dolla C,Balaji S,Baskaran D,Thiruvengadam K,Rajendran A,Narayanan S,Raj D,Swaminathan S,Peacock SJ

    更新日期:2019-11-29 00:00:00

  • Experimental challenge of pregnant cattle with the putative abortifacient Waddlia chondrophila.

    abstract::Waddlia chondrophila is a Gram-negative intracellular bacterial organism that is related to classical chlamydial species and has been implicated as a cause of abortion in cattle. Despite an increasing number of observational studies linking W. chondrophila infection to cattle abortion, little direct experimental evide...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/srep37150

    authors: Wheelhouse N,Flockhart A,Aitchison K,Livingstone M,Finlayson J,Flachon V,Sellal E,Dagleish MP,Longbottom D

    更新日期:2016-11-14 00:00:00

  • Microbiome of Pacific Whiteleg shrimp reveals differential bacterial community composition between Wild, Aquacultured and AHPND/EMS outbreak conditions.

    abstract::Crustaceans form the second largest subphylum on Earth, which includes Litopeneaus vannamei (Pacific whiteleg shrimp), one of the most cultured shrimp worldwide. Despite efforts to study the shrimp microbiota, little is known about it from shrimp obtained from the open sea and the role that aquaculture plays in microb...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-017-11805-w

    authors: Cornejo-Granados F,Lopez-Zavala AA,Gallardo-Becerra L,Mendoza-Vargas A,Sánchez F,Vichido R,Brieba LG,Viana MT,Sotelo-Mundo RR,Ochoa-Leyva A

    更新日期:2017-09-18 00:00:00

  • Toll like receptor7 polymorphisms in relation to disease susceptibility and progression in Chinese patients with chronic HBV infection.

    abstract::Toll-like receptors (TLRs) play a key role in innate and adaptive immunity, protecting the host from viral pathogens. We studied the effect of TLR7 polymorphisms on disease susceptibility and progression of chronic hepatitis B (CHB) infection in Chinese adults. Blood samples were taken from 612 patients with confirmed...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-017-12698-5

    authors: Zhu J,Zhang T,Cao L,Li A,Zheng K,Zhang N,Su B,Chen Z,Chen N,Wu H,He Q

    更新日期:2017-09-29 00:00:00

  • Emergence of life in an inflationary universe.

    abstract::Abiotic emergence of ordered information stored in the form of RNA is an important unresolved problem concerning the origin of life. A polymer longer than 40-100 nucleotides is necessary to expect a self-replicating activity, but the formation of such a long polymer having a correct nucleotide sequence by random react...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-020-58060-0

    authors: Totani T

    更新日期:2020-02-03 00:00:00

  • A Canonical Biophysical Model of the CsrA Global Regulator Suggests Flexible Regulator-Target Interactions.

    abstract::Bacterial global post-transcriptional regulators execute hundreds of interactions with targets that display varying molecular features while retaining specificity. Herein, we develop, validate, and apply a biophysical, statistical thermodynamic model of canonical target mRNA interactions with the CsrA global post-tran...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-018-27474-2

    authors: Leistra AN,Gelderman G,Sowa SW,Moon-Walker A,Salis HM,Contreras LM

    更新日期:2018-07-02 00:00:00

  • Lateral Force Microscopy of Interfacial Nanobubbles: Friction Reduction and Novel Frictional Behavior.

    abstract::Atomic force microscopy is used to conduct single-asperity friction measurements at a water-graphite interface. Local mapping of the frictional force, which is based on the degree of the cantilever twisting, shows nearly friction-free when a tip scans over a nanobubble. Surprisingly, apart from being gapless, the asso...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-018-21264-6

    authors: Yang CW,Leung KT,Ding RF,Ko HC,Lu YH,Fang CK,Hwang IS

    更新日期:2018-02-15 00:00:00