Abstract:
:While implicated in therapeutic resistance, malignant progenitor cell cycle kinetics have been difficult to quantify in real-time. We developed an efficient lentiviral bicistronic fluorescent, ubiquitination-based cell cycle indicator reporter (Fucci2BL) to image live single progenitors on a defined niche coupled with cell cycle gene expression analysis. We have identified key differences in cell cycle regulatory gene expression and transit times between normal and chronic myeloid leukemia progenitors that may inform cancer stem cell eradication strategies.
journal_name
Sci Repjournal_title
Scientific reportsauthors
Pineda G,Lennon KM,Delos Santos NP,Lambert-Fliszar F,Riso GL,Lazzari E,Marra MA,Morris S,Sakaue-Sawano A,Miyawaki A,Jamieson CHdoi
10.1038/srep23885subject
Has Abstractpub_date
2016-04-04 00:00:00pages
23885issn
2045-2322pii
srep23885journal_volume
6pub_type
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