Suprabasin-derived bioactive peptides identified by plasma peptidomics.

Abstract:

:Identification of low-abundance, low-molecular-weight native peptides using non-tryptic plasma has long remained an unmet challenge, leaving potential bioactive/biomarker peptides undiscovered. We have succeeded in efficiently removing high-abundance plasma proteins to enrich and comprehensively identify low-molecular-weight native peptides using mass spectrometry. Native peptide sequences were chemically synthesized and subsequent functional analyses resulted in the discovery of three novel bioactive polypeptides derived from an epidermal differentiation marker protein, suprabasin. SBSN_HUMAN[279-295] potently suppressed food/water intake and induced locomotor activity when injected intraperitoneally, while SBSN_HUMAN[225-237] and SBSN_HUMAN[243-259] stimulated the expression of proinflammatory cytokines via activation of NF-κB signaling in vascular cells. SBSN_HUMAN[225-237] and SBSN_HUMAN[279-295] immunoreactivities were present in almost all human organs analyzed, while immunoreactive SBSN_HUMAN[243-259] was abundant in the liver and pancreas. Human macrophages expressed the three suprabasin-derived peptides. This study illustrates a new approach for discovering unknown bioactive peptides in plasma via the generation of peptide libraries using a novel peptidomic strategy.

journal_name

Sci Rep

journal_title

Scientific reports

authors

Taguchi T,Kodera Y,Oba K,Saito T,Nakagawa Y,Kawashima Y,Shichiri M

doi

10.1038/s41598-020-79353-4

subject

Has Abstract

pub_date

2021-01-13 00:00:00

pages

1047

issue

1

issn

2045-2322

pii

10.1038/s41598-020-79353-4

journal_volume

11

pub_type

杂志文章
  • Experimental evaluation of the impact of household aerosolized insecticides on pyrethroid resistant Aedes aegypti.

    abstract::The extensive reliance on insecticides to control Aedes aegypti mosquitoes and disrupt transmission of dengue, chikungunya and Zika has fueled the emergence of widespread resistance to insecticides. Mismatch between the frequency of pyrethroid resistance in mosquitoes and the occurrence of pyrethroid-based insecticide...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-018-30968-8

    authors: Gray L,Florez SD,Barreiro AM,Vadillo-Sánchez J,González-Olvera G,Lenhart A,Manrique-Saide P,Vazquez-Prokopec GM

    更新日期:2018-08-22 00:00:00

  • Comparison of DNA repair and radiosensitivity of different blood cell populations.

    abstract::Despite the frequent use of ionising radiation (IR) in therapy and diagnostics and the unavoidable exposure to external radiation sources, our knowledge regarding the radiosensitivity of human blood cell populations is limited and published data, obtained under different experimental conditions, are heterogeneous. To ...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-021-81058-1

    authors: Heylmann D,Ponath V,Kindler T,Kaina B

    更新日期:2021-01-28 00:00:00

  • Electrochemical deposition of layered copper thin films based on the diffusion limited aggregation.

    abstract::In this work layered copper films with smooth surface were successfully fabricated onto ITO substrate by electrochemical deposition (ECD) and the thickness of the films was nearly 60 nm. The resulting films were characterized by SEM, TEM, AFM, XPS, and XRD. We have investigated the effects of potential and the concent...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/srep34779

    authors: Wei C,Wu G,Yang S,Liu Q

    更新日期:2016-10-13 00:00:00

  • Wetland salinity induces sex-dependent carry-over effects on the individual performance of a long-distance migrant.

    abstract::Salinization is having a major impact on wetlands and its biota worldwide. Specifically, many migratory animals that rely on wetlands are increasingly exposed to elevated salinity on their nonbreeding grounds. Experimental evidence suggests that physiological challenges associated with increasing salinity may disrupt ...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-017-07258-w

    authors: Masero JA,Abad-Gómez JM,Gutiérrez JS,Santiago-Quesada F,Senner NR,Sánchez-Guzmán JM,Piersma T,Schroeder J,Amat JA,Villegas A

    更新日期:2017-07-31 00:00:00

  • Correlative nonlinear optical microscopy and infrared nanoscopy reveals collagen degradation in altered parchments.

    abstract::This paper presents the correlative imaging of collagen denaturation by nonlinear optical microscopy (NLO) and nanoscale infrared (IR) spectroscopy to obtain morphological and chemical information at different length scales. Such multiscale correlated measurements are applied to the investigation of ancient parchments...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/srep26344

    authors: Latour G,Robinet L,Dazzi A,Portier F,Deniset-Besseau A,Schanne-Klein MC

    更新日期:2016-05-19 00:00:00

  • Field-Dependent Heat Dissipation of Carbon Nanotube Electric Currents.

    abstract::We study heat dissipation of a multi-wall carbon nanotube (MWCNT) device fabricated from two crossed nanotubes on a SiNx substrate under the influence of a constant (DC) electric bias. By monitoring the temperature of the substrate, we observe negligible Joule heating within the nanotube lattice itself and instead hea...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-019-46944-9

    authors: Voskanian N,Olsson E,Cumings J

    更新日期:2019-07-25 00:00:00

  • Psychological Profile in Coronary Artery By-Pass Graft Patients vs. Valve Replacement Patients Entering Cardiac Rehabilitation after Surgery.

    abstract::Anxiety and depression are thought to influence the genesis of ischemic diseases and not of valvular diseases, but little is known on the psychological profile of cardiac patients after surgery. Aim of this study was to investigate differences in disease experience and mood between patients undergoing cardiac rehabili...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-018-32696-5

    authors: Modica M,Castiglioni P,Minotti A,Faini A,Racca V,Ferratini M

    更新日期:2018-09-26 00:00:00

  • Analysis of Corneal Spherical Aberrations in Cataract Patients with High Myopia.

    abstract::To evaluate the corneal spherical aberrations in cataract patients with and without high myopia, we conducted a retrospective case series of 502 cataract eyes with high myopia and 1500 age-related cataract eyes and measure their corneal biometric data and axial length using Pentacam and IOLMaster. Both the anterior an...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-018-36539-1

    authors: Zhang M,Qian D,Jing Q,Chen J,Jiang Y

    更新日期:2019-02-05 00:00:00

  • Single molecule super-resolution imaging of bacterial cell pole proteins with high-throughput quantitative analysis pipeline.

    abstract::Bacteria show sophisticated control of their cellular organization, and many bacteria deploy different polar landmark proteins to organize the cell pole. Super-resolution microscopy, such as Photo-Activated Localization Microscopy (PALM), provides the nanoscale localization of molecules and is crucial for better under...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-019-43051-7

    authors: Altinoglu I,Merrifield CJ,Yamaichi Y

    更新日期:2019-04-30 00:00:00

  • Integrated transcriptomic and proteomic analyses of a molecular mechanism of radular teeth biomineralization in Cryptochiton stelleri.

    abstract::Many species of chiton are known to deposit magnetite (Fe3O4) within the cusps of their heavily mineralized and ultrahard radular teeth. Recently, much attention has been paid to the ultrastructural design and superior mechanical properties of these radular teeth, providing a promising model for the development of nov...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-018-37839-2

    authors: Nemoto M,Ren D,Herrera S,Pan S,Tamura T,Inagaki K,Kisailus D

    更新日期:2019-01-29 00:00:00

  • Body mass index in young men and risk of inflammatory bowel disease through adult life: A population-based Danish cohort study.

    abstract::Body mass index (BMI) is associated with increased future risk of inflammatory bowel disease(IBD) particularly Crohn's disease(CD), where associations with high and low BMI have been observed. Most studies are based on adult women. We aimed to explore the impact of BMI in men entering adult life on their long-term ris...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-019-42642-8

    authors: Mendall MA,Jensen CB,Sørensen TIA,Ängquist LH,Jess T

    更新日期:2019-04-23 00:00:00

  • Long-term T cell fitness and proliferation is driven by AMPK-dependent regulation of reactive oxygen species.

    abstract::The AMP-activated kinase (AMPK) is a major energy sensor metabolic enzyme that is activated early during T cell immune responses but its role in the generation of effector T cells is still controversial. Using both in vitro and in vivo models of T cell proliferation, we show herein that AMPK is dispensable for early T...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-020-78715-2

    authors: Lepez A,Pirnay T,Denanglaire S,Perez-Morga D,Vermeersch M,Leo O,Andris F

    更新日期:2020-12-10 00:00:00

  • Positive/Negative Allosteric Modulation Switching in an Umami Taste Receptor (T1R1/T1R3) by a Natural Flavor Compound, Methional.

    abstract::Taste is a vital sensation for vertebrates, enabling the detection of nutritionally important substances or potential toxins. A heteromeric complex of two class C GPCRs, T1R1 and T1R3, was identified as the umami (savory) taste receptor. Amino acids and 5'-ribonucleotides are well known to be natural ligands for human...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-018-30315-x

    authors: Toda Y,Nakagita T,Hirokawa T,Yamashita Y,Nakajima A,Narukawa M,Ishimaru Y,Uchida R,Misaka T

    更新日期:2018-08-07 00:00:00

  • Functional expression and pharmaceutical efficacy of cardiac-specific ion channels in human embryonic stem cell-derived cardiomyocytes.

    abstract::Cardiomyocytes differentiated from human pluripotent stem cells provide promising tools for screening of cardiotoxic drugs. For evaluation of human pluripotent stem cell-derived cardiomyocytes for cardiotoxicity test, in the present study, human embryonic stem cells (hESCs) were differentiated to cardiomyocytes, follo...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-017-14198-y

    authors: Kim HS,Yoon JW,Li H,Jeong GO,Park JJ,Shin SE,Jang IH,Kim JH,Park WS

    更新日期:2017-10-23 00:00:00

  • Biocompatibility and biodegradation studies of a commercial zinc alloy for temporary mini-implant applications.

    abstract::In this study, the biocompatibility and in vitro degradation behaviour of a commercial zinc-based alloy (Zn-5 Al-4 Mg) were evaluated and compared with that of pure zinc for temporary orthopaedic implant applications. Biocompatibility tests were conducted using human alveolar lung epithelial cells (A549), which showed...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-017-15873-w

    authors: Kannan MB,Moore C,Saptarshi S,Somasundaram S,Rahuma M,Lopata AL

    更新日期:2017-11-15 00:00:00

  • Author Correction: Transcriptomic changes in the pre-implantation uterus highlight histotrophic nutrition of the developing marsupial embryo.

    abstract::An amendment to this paper has been published and can be accessed via a link at the top of the paper. ...

    journal_title:Scientific reports

    pub_type: 已发布勘误

    doi:10.1038/s41598-020-68759-9

    authors: Whittington CM,O'Meally D,Laird MK,Belov K,Thompson MB,McAllan BM

    更新日期:2020-07-22 00:00:00

  • Microbial community analysis and biodeterioration of waterlogged archaeological wood from the Nanhai No. 1 shipwreck during storage.

    abstract::Wooden shipwrecks are a significant part of the underwater cultural heritage. In 2007, the Nanhai No. 1 shipwreck was salvaged from the seabed and moved into the Marine Silk Road Museum, where it is still stored in a water tank. We analysed the microbial communities colonizing the hull surface of the Nanhai No. 1 ship...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-018-25484-8

    authors: Liu Z,Fu T,Hu C,Shen D,Macchioni N,Sozzi L,Chen Y,Liu J,Tian X,Ge Q,Feng Z,Liu H,Zhang Z,Pan J

    更新日期:2018-05-08 00:00:00

  • Formulating formation mechanism of natural gas hydrates.

    abstract::A large amount of energy, perhaps twice the total amount of all other hydrocarbon reserves combined, is trapped within gas hydrate deposits. Despite emerging as a potential energy source for the world over the next several hundred years and one of the key factors in causing future climate change, gas hydrate is poorly...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-017-06717-8

    authors: Palodkar AV,Jana AK

    更新日期:2017-07-25 00:00:00

  • Growth of epitaxial silicon nanowires on a Si substrate by a metal-catalyst-free process.

    abstract::The growth of epitaxial Si nanowires by a metal-catalyst-free process has been investigated as an alternative to the more common metal-catalyzed vapor-liquid-solid process. The well-aligned Si nanowires are successfully grown on a (111)-oriented Si substrate without any metal catalysts by a thermal treatment using sil...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/srep30608

    authors: Ishiyama T,Nakagawa S,Wakamatsu T

    更新日期:2016-07-28 00:00:00

  • Microparticles controllable accumulation, arrangement, and spatial shaping performed by tapered-fiber-based laser-induced convection flow.

    abstract::The ability to arrange cells and/or microparticles into the desired pattern is critical in biological, chemical, and metamaterial studies and other applications. Researchers have developed a variety of patterning techniques, which either have a limited capacity to simultaneously trap massive particles or lack the spat...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-017-14802-1

    authors: Zhang Y,Lei J,Zhang Y,Liu Z,Zhang J,Yang X,Yang J,Yuan L

    更新日期:2017-10-30 00:00:00

  • Climate- and gateway-driven cooling of Late Eocene to earliest Oligocene sea surface temperatures in the North Sea Basin.

    abstract::During the late Eocene, the Earth's climate experienced several transient temperature fluctuations including the Vonhof cooling event (C16n.1n; ~35.8 Ma) hitherto known mainly from the southern oceans. Here we reconstruct sea-surface temperatures (SST) and provide δ18O and δ13C foraminiferal records for the late Eocen...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-019-41013-7

    authors: Śliwińska KK,Thomsen E,Schouten S,Schoon PL,Heilmann-Clausen C

    更新日期:2019-03-14 00:00:00

  • Fukushima radionuclides in the NW Pacific, and assessment of doses for Japanese and world population from ingestion of seafood.

    abstract::Variations of Fukushima-derived radionuclides ((90)Sr, (134)Cs and (137)Cs) in seawater and biota offshore Fukushima and in the NW Pacific Ocean were investigated and radiation doses to the Japanese and world population from ingestion of seafood contaminated by Fukushima radionuclides were estimated and compared with ...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/srep09016

    authors: Povinec PP,Hirose K

    更新日期:2015-03-12 00:00:00

  • Ethnicity and anticoagulation management of hospitalized patients with atrial fibrillation in northwest China.

    abstract::The therapeutic management and health challenges caused by atrial fibrillation (AF) differ between different groups. The purpose of this study was to investigate the clinical features of patients hospitalized with AF and to explore the use of anticoagulation treatments in Han and Uygur patients in Xinjiang, northwest ...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/srep45884

    authors: Cheng X,Zhou X,Song S,Wu M,Baolatejiang R,Lu Y,Li Y,Zhang W,Lv W,Ye Y,Zhou Q,Wang H,Zhang J,Xing Q,Tang B

    更新日期:2017-04-10 00:00:00

  • A Boolean Model of Microvascular Rarefaction to Predict Treatment Outcomes in Renal Disease.

    abstract::Despite advances in renovascular disease (RVD) research, gaps remain between experimental and clinical outcomes, translation of results, and the understanding of pathophysiological mechanisms. A predictive tool to indicate support (or lack of) for biological findings may aid clinical translation of therapies. We creat...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-019-57386-8

    authors: Williams E,Chade AR

    更新日期:2020-01-16 00:00:00

  • Comprehensive identification of the full-length transcripts and alternative splicing related to the secondary metabolism pathways in the tea plant (Camellia sinensis).

    abstract::Flavonoids, theanine and caffeine are the main secondary metabolites of the tea plant (Camellia sinensis), which account for the tea's unique flavor quality and health benefits. The biosynthesis pathways of these metabolites have been extensively studied at the transcriptional level, but the regulatory mechanisms are ...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-019-39286-z

    authors: Qiao D,Yang C,Chen J,Guo Y,Li Y,Niu S,Cao K,Chen Z

    更新日期:2019-02-25 00:00:00

  • Continuous leaky-wave scanning using periodically modulated spoof plasmonic waveguide.

    abstract::The plasmonic waveguide made of uniform corrugated metallic strip can support and guide spoof surface plasmon polaritons (SSPPs) with high confinements. Here, we propose periodically-modulated plasmonic waveguide composed of non-uniform corrugated metallic strip to convert SSPPs to radiating waves, in which the main b...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/srep29600

    authors: Kong GS,Ma HF,Cai BG,Cui TJ

    更新日期:2016-07-12 00:00:00

  • Characterisation of a flavonoid ligand of the fungal protein Alt a 1.

    abstract::Spores of pathogenic fungi are virtually ubiquitous and cause human disease and severe losses in crops. The endophytic fungi Alternaria species produce host-selective phytotoxins. Alt a 1 is a strongly allergenic protein found in A. alternata that causes severe asthma. Despite the well-established pathogenicity of Alt...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/srep33468

    authors: Garrido-Arandia M,Silva-Navas J,Ramírez-Castillejo C,Cubells-Baeza N,Gómez-Casado C,Barber D,Pozo JC,Melendi PG,Pacios LF,Díaz-Perales A

    更新日期:2016-09-16 00:00:00

  • Alleviation of depression-like behavior in a cystic fibrosis mouse model by Hdac6 depletion.

    abstract::Cystic fibrosis (CF) patients experience heightened levels of anxiety and depression. Stress from dealing with chronic disease and rigorous treatment regimens certainly are primary contributors to these outcomes. We previously have demonstrated that microtubule alterations in CF are linked to a number of CF phenotypes...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-020-73298-4

    authors: Corey DA,Rymut SM,Kelley TJ

    更新日期:2020-10-01 00:00:00

  • Identification of hybrid node and link communities in complex networks.

    abstract::Identifying communities in complex networks is an effective means for analyzing complex systems, with applications in diverse areas such as social science, engineering, biology and medicine. Finding communities of nodes and finding communities of links are two popular schemes for network analysis. These schemes, howev...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/srep08638

    authors: He D,Jin D,Chen Z,Zhang W

    更新日期:2015-03-02 00:00:00

  • Membrane bound IL-21 based NK cell feeder cells drive robust expansion and metabolic activation of NK cells.

    abstract::NK cell adoptive therapy is a promising cancer therapeutic approach, but there are significant challenges that limiting its feasibility and clinical efficacy. One difficulty is the paucity of clinical grade manufacturing platforms to support the large scale expansion of highly active NK cells. We created an NK cell fe...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-019-51287-6

    authors: Ojo EO,Sharma AA,Liu R,Moreton S,Checkley-Luttge MA,Gupta K,Lee G,Lee DA,Otegbeye F,Sekaly RP,de Lima M,Wald DN

    更新日期:2019-10-17 00:00:00