Abstract：
:Probing the oligomeric state of abundant molecules, such as membrane proteins in intact cells, is essential, but has not been straightforward. We address this challenge with a simple counting strategy that is capable of reporting the oligomeric state of dense, membrane-bound protein complexes. It is based on single-molecule localization microscopy to super-resolve protein structures in intact cells and basic quantitative evaluation. We validate our method with membrane-bound monomeric CD86 and dimeric cytotoxic T-lymphocyte-associated protein as model proteins and confirm their oligomeric states. We further detect oligomerization of CD80 and vesicular stomatitis virus glycoprotein and propose coexistence of monomers and dimers for CD80 and trimeric assembly of the viral protein at the cell membrane. This approach should prove valuable for researchers striving for reliable molecular counting in cells.
journal_name
Sci Repjournal_title
Scientific reportsauthors
Fricke F,Beaudouin J,Eils R,Heilemann Mdoi
10.1038/srep14072subject
Has Abstractpub_date
2015-09-11 00:00:00pages
14072issn
2045-2322pii
srep14072journal_volume
5pub_type
杂志文章abstract：:Excessive activation of inflammation and the accompanying lung vascular endothelial barrier disruption are primary pathogenic features of acute lung injury (ALI). Microtubule-associated protein 4 (MAP4), a tubulin assembly-promoting protein, is important for maintaining the microtubule (MT) cytoskeleton and cell-cell ...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep08895
更新日期：2015-03-09 00:00:00
abstract：:Reactive Intermediate Deaminase (Rid) protein superfamily includes eight families among which the RidA is conserved in all domains of life. RidA proteins accelerate the deamination of the reactive 2-aminoacrylate (2AA), an enamine produced by some pyridoxal phosphate (PLP)-dependent enzymes. 2AA accumulation inhibits ...
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pub_type： 杂志文章
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更新日期：2020-06-23 00:00:00
abstract：:Liver stage Plasmodium parasites reside in a parasitophorous vacuole (PV) that associates with lysosomes. It has previously been shown that these organelles can have beneficial as well as harmful effects on the parasite. Yet it is not clear how the association of lysosomes with the parasite is controlled and how inter...
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pub_type： 杂志文章
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更新日期：2019-05-28 00:00:00
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journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep28928
更新日期：2016-06-29 00:00:00
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pub_type： 杂志文章
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pub_type： 杂志文章
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pub_type： 杂志文章
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更新日期：2016-08-31 00:00:00
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pub_type： 杂志文章
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更新日期：2016-12-20 00:00:00
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pub_type： 杂志文章
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pub_type： 杂志文章
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更新日期：2018-03-15 00:00:00
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pub_type： 杂志文章
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更新日期：2016-08-23 00:00:00
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pub_type： 杂志文章
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更新日期：2013-10-15 00:00:00
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journal_title：Scientific reports
pub_type： 杂志文章
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更新日期：2019-09-09 00:00:00
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journal_title：Scientific reports
pub_type： 杂志文章
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更新日期：2019-11-13 00:00:00
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pub_type： 杂志文章
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更新日期：2021-01-15 00:00:00
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journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep02871
更新日期：2013-10-16 00:00:00
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journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-79686-0
更新日期：2020-12-29 00:00:00
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journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep00476
更新日期：2012-01-01 00:00:00
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journal_title：Scientific reports
pub_type： 杂志文章
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更新日期：2017-02-06 00:00:00
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journal_title：Scientific reports
pub_type： 杂志文章
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更新日期：2019-08-22 00:00:00
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pub_type： 杂志文章
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更新日期：2018-04-03 00:00:00
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journal_title：Scientific reports
pub_type： 杂志文章
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更新日期：2019-02-27 00:00:00
abstract：:The aim was to investigate the ability of self-assembling Peptide P11-4 Matrix (SAPM) to remineralize artificial initial caries lesions compared to the use of fluoride varnish. Volunteers were recruited for this randomised, cross-over in situ trial. Bovine specimens, half including orthodontic brackets, were recessed ...
journal_title：Scientific reports
pub_type： 杂志文章,随机对照试验
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更新日期：2019-01-22 00:00:00
abstract：:Nonalcoholic fatty liver disease (NAFLD) is an important risk factor for the development of type 2 diabetes mellitus. Interferon gamma-induced protein 10 (IP-10), a proinflammatory chemokine, plays a crucial role in inflammatory diseases. This cross-sectional pilot study investigated whether circulating IP-10 is assoc...
journal_title：Scientific reports
pub_type： 临床试验,杂志文章,多中心研究
doi：10.1038/srep10096
更新日期：2015-05-11 00:00:00
abstract：:Indoor air quality (IAQ) is much more crucial to human health than its atmospheric air quality counterpart. Improving indoor air environment requires investigating how different indoor air stability affects airflow trajectory. By presenting both manikin experiment and Computational Fluid Dynamics (CFD) simulation, we ...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-017-08871-5
更新日期：2017-08-17 00:00:00
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journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-018-31069-2
更新日期：2018-09-04 00:00:00
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journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep34466
更新日期：2016-10-06 00:00:00
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journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-46934-x
更新日期：2019-07-18 00:00:00
abstract：:Cold agglutinin disease (CAD) is a rare form of autoimmune haemolytic anaemia, and because of its rareness, there is no standard treatment for CAD patients. We retrospectively analysed the response to rituximab-containing therapy in CAD patients at our hospital. All patients received rituximab-containing therapy for a...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-69465-2
更新日期：2020-07-29 00:00:00
