当前位置： SCI文献检索 > INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES期刊下所有文献 > BmajPLA2-II, a basic Lys49-phospholipase A2 homologue from Bothrops marajoensis snake venom with parasiticidal potential.

BmajPLA2-II, a basic Lys49-phospholipase A2 homologue from Bothrops marajoensis snake venom with parasiticidal potential.

Abstract：

:Snake venoms contain various proteins, especially phospholipases A2 (PLA2s), which present potential applications in diverse areas of health and medicine. In this study, a new basic PLA2 from Bothrops marajoensis with parasiticidal activity was purified and characterized biochemically and biologically. B. marajoensis venom was fractionated through cation exchange followed by reverse phase chromatographies. The isolated toxin, BmajPLA2-II, was structurally characterized with MALDI-TOF (Matrix-assisted laser desorption/ionization-time of flight) mass spectrometry, sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE), followed by two-dimensional electrophoresis, partial amino acid sequencing, an enzymatic activity assay, circular dichroism, and dynamic light scattering assays. These structural characterization tests presented BmajPLA2-II as a basic Lys49 PLA2 homologue, compatible with other basic snake venom PLA2s (svPLA2), with a tendency to form aggregations. The in vitro anti-parasitic potential of B. marajoensis venom and of BmajPLA2-II was evaluated against Leishmania infantum promastigotes and Trypanosoma cruzi epimastigotes, showing significant activity at a concentration of 100μg/mL. The venom and BmajPLA2-II presented IC50 of 0.14±0.08 and 6.41±0.64μg/mL, respectively, against intraerythrocytic forms of Plasmodium falciparum with CC50 cytotoxicity values against HepG2 cells of 43.64±7.94 and >150μg/mL, respectively. The biotechnological potential of these substances in relation to leishmaniasis, Chagas disease and malaria should be more deeply investigated.

journal_name

Int J Biol Macromol

journal_title

International journal of biological macromolecules

authors

Grabner AN,Alfonso J,Kayano AM,Moreira-Dill LS,Dos Santos APA,Caldeira CAS,Sobrinho JC,Gómez A,Grabner FP,Cardoso FF,Zuliani JP,Fontes MRM,Pimenta DC,Gómez CV,Teles CBG,Soares AM,Calderon LA

doi

10.1016/j.ijbiomac.2017.04.013

subject

Has Abstract

pub_date

2017-09-01 00:00:00

pages

571-581

eissn

0141-8130

issn

1879-0003

pii

S0141-8130(16)30677-8

journal_volume

102

pub_type

杂志文章

相关文献

INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES文献大全
  • Improved pullulan production by a mutant of Aureobasidium melanogenum TN3-1 from a natural honey and capsule shell preparation.

    abstract：:Aureobasidium melanogenum TN3-1 isolated from a natural honey was a highly genome-duplicated yeast-like fungal strain and a very high pullulan producer. In this study, simultaneous removal of both duplicated AMY1 genes encoding α-amylase and duplicated PKS1 genes responsible for melanin biosynthesis in A. melanogenum ...

    journal_title：International journal of biological macromolecules

    pub_type： 杂志文章

    doi：10.1016/j.ijbiomac.2019.08.264

    authors： Chen L,Chi Z,Liu GL,Xue SJ,Wang ZP,Hu Z,Chi ZM

    更新日期：2019-12-01 00:00:00

  • Physiometabolic effects of Agave salmiana fructans evaluated in Wistar rats.

    abstract：:A prospective completely randomized experimental study was conducted using 48 animals to evaluate the physiometabolic effects of Agave salmiana fructans as a dietary supplement in healthy Wistar rats. Five fructans concentrations from 5 to 20% (w/w) and one control were used in the rats' diet and were divided into six...

    journal_title：International journal of biological macromolecules

    pub_type： 杂志文章

    doi：10.1016/j.ijbiomac.2017.11.043

    authors： Castillo Andrade AI,Rivera Bautista C,Godínez Hernández C,Ruiz Cabrera MA,Fuentes Ahumada C,García Chávez E,Grajales Lagunes A

    更新日期：2018-03-01 00:00:00

  • High-performance interactive analysis of split aptamer and HIV-1 Tat on multiwall carbon nanotube-modified field-effect transistor.

    abstract：:Interaction between split RNA aptamer and the clinically important target, HIV-1 Tat was investigated on a biosensing surface transduced by functionally choreographed multiwall carbon nanotubes (MWCNTs). Acid oxidation was performed to functionalize MWCNTs with carboxyl functional groups. X-ray photoelectron spectrosc...

    journal_title：International journal of biological macromolecules

    pub_type： 杂志文章

    doi：10.1016/j.ijbiomac.2018.12.066

    authors： Fatin MF,Rahim Ruslinda A,Gopinath SCB,Arshad MKM

    更新日期：2019-03-15 00:00:00

  • Effect of Curcuma kwangsiensis polysaccharides on blood lipid profiles and oxidative stress in high-fat rats.

    abstract：:We have investigated the protective effect of polysaccharides from Curcuma kwangsiensis (CKP) against oxidative injury in rats fed high-fat diet. The protective effect of CKP was compared with Lovastatin, a well-known antioxidant. Sixty SD rats were used for the experimental study. Oxidative injury was induced by feed...

    journal_title：International journal of biological macromolecules

    pub_type： 杂志文章

    doi：10.1016/j.ijbiomac.2008.11.005

    authors： Xu C,Haiyan Z,Hua Z,Jianhong Z,Pin D

    更新日期：2009-03-01 00:00:00

  • Starch based biodegradable graft copolymer for the preparation of silver nanoparticles.

    abstract：:The synthesis and characterization of a novel biodegradable graft copolymer based on partially hydrolyzed polymethylacrylate (PMA) grafted amylopectin (AP) was reported which was developed for the synthesis of silver nanoparticles from silver nitrate solution by facile green technique. The prepared graft copolymer was...

    journal_title：International journal of biological macromolecules

    pub_type： 杂志文章

    doi：10.1016/j.ijbiomac.2015.07.046

    authors： Das S,Sasmal D,Pal S,Kolya H,Pandey A,Tripathy T

    更新日期：2015-11-01 00:00:00

  • Binding of bivalent cations by xanthan in aqueous solution.

    abstract：:The interaction between xanthan and selected bivalent cations (Ca2+, Mg2+, Mn2+, Fe2+, Cu2+, Zn2+, Cd2+ and Pb2+) was studied by means of conductometry, viscometry, and nuclear magnetic resonance spectroscopy. Xanthan from Xanthomonas campestris was studied in comparison with dextran from Leuconostoc mesenteroides. Wh...

    journal_title：International journal of biological macromolecules

    pub_type： 杂志文章

    doi：10.1016/j.ijbiomac.2008.06.001

    authors： Bergmann D,Furth G,Mayer C

    更新日期：2008-10-01 00:00:00

  • Development of the PVA/CS nanofibers containing silk protein sericin as a wound dressing: In vitro and in vivo assessment.

    abstract：:Skin and soft tissue infections are major concerns with respect to wound repair. Recently, anti-bacterial wound dressings have been emerging as promising candidates to reduce infection, thus accelerating the wound healing process. This paper presents our work to develop and characterize poly(vinyl alcohol) (PVA)/chito...

    journal_title：International journal of biological macromolecules

    pub_type： 杂志文章

    doi：10.1016/j.ijbiomac.2020.01.139

    authors： Bakhsheshi-Rad HR,Ismail AF,Aziz M,Akbari M,Hadisi Z,Omidi M,Chen X

    更新日期：2020-04-15 00:00:00

  • High yield production of levan-type fructans by Gluconobacter japonicus LMG 1417.

    abstract：:Levan, a β-2,6-glycosidic linked fructan, is a promising alternative for the inulin dominated fructan market. Although levan is already used in some cosmetic products, the commercial availability of the fructan is still limited. Here we show that Gluconobacter japonicus LMG 1417 is a potent levan-forming organism and ...

    journal_title：International journal of biological macromolecules

    pub_type： 杂志文章

    doi：10.1016/j.ijbiomac.2020.07.105

    authors： Hövels M,Kosciow K,Kniewel J,Jakob F,Deppenmeier U

    更新日期：2020-12-01 00:00:00

  • Optimization of the culture conditions for production of Polyhydroxyalkanoate and its characterization from a new Bacillus cereus sp. BNPI-92 strain, isolated from plastic waste dumping yard.

    abstract：:The aim of this study was to optimize the culture conditions for production, recovery and characterization of polyhydroxyalkanoate (PHA) from potential Bacillus cereus strain BNPI-92. Cost effective carbon sources such as crude oil (CO), rice bran (RB), sugar cane molasses (SCM) and wheat bran (WB) were evaluated for ...

    journal_title：International journal of biological macromolecules

    pub_type： 杂志文章

    doi：10.1016/j.ijbiomac.2019.11.138

    authors： Mohammed S,Behera HT,Dekebo A,Ray L

    更新日期：2020-08-01 00:00:00

  • Ovotransferrin enhances intestinal immune response in cyclophosphamide-induced immunosuppressed mice.

    abstract：:Ovotransferrin (OVT), a glycoprotein from avian egg, which has a variety of biological activities and immunomodulatory effects. The purpose of this research was to demonstrate the effect of OVT on intestinal immunomodulatory function which used a mouse model of cyclophosphamide (CP) induced intestinal immunosuppressio...

    journal_title：International journal of biological macromolecules

    pub_type： 杂志文章

    doi：10.1016/j.ijbiomac.2018.08.058

    authors： Zhu G,Luo J,Du H,Jiang Y,Tu Y,Yao Y,Xu M

    更新日期：2018-12-01 00:00:00

  • Structural features and in vivo antitussive activity of the water extracted polymer from Glycyrrhiza glabra.

    abstract：:Antitussive drugs are amongst the most widely used medications worldwide; however no new class of drugs has been introduced into the market for many years. Herein, we have analyzed the water-extracted polymeric fraction (WE) of Glycyrrhiza glabra. This arabinogalactan protein enriched fraction, ≥ 85% of which gets pre...

    journal_title：International journal of biological macromolecules

    pub_type： 杂志文章

    doi：10.1016/j.ijbiomac.2011.02.003

    authors： Saha S,Nosál'ová G,Ghosh D,Flešková D,Capek P,Ray B

    更新日期：2011-05-01 00:00:00

  • Effects of the ultra-high pressure on structure and α-glucosidase inhibition of polysaccharide from Astragalus.

    abstract：:A novel homogeneous polysaccharide fraction (APS) was extracted from Astragalus by hot water and purified by Sephadex G-100 and G-75 column. Its molecular weight was 693kDa. APS and APS with ultra-high pressure treatment exhibited significant inhibitory abilities on a-glucosidase, inhibition rate from high to low in o...

    journal_title：International journal of biological macromolecules

    pub_type： 杂志文章

    doi：10.1016/j.ijbiomac.2016.03.024

    authors： Zhu ZY,Luo Y,Dong GL,Ren YY,Chen LJ,Guo MZ,Wang XT,Yang XY,Zhang Y

    更新日期：2016-06-01 00:00:00

  • Microcrystalline cellulose: Isolation, characterization and bio-composites application-A review.

    abstract：:Considering its widespread usage in various fields, such as food, pharmaceutical, medical, cosmetic and polymer composites industries, microcrystalline cellulose (MCC) is becoming impellent due to increasing demand of alternatives to non-renewable and scarce fossil materials. Although it still suffers from some drawba...

    journal_title：International journal of biological macromolecules

    pub_type： 杂志文章,评审

    doi：10.1016/j.ijbiomac.2016.09.056

    authors： Trache D,Hussin MH,Hui Chuin CT,Sabar S,Fazita MR,Taiwo OF,Hassan TM,Haafiz MK

    更新日期：2016-12-01 00:00:00

  • Purification, structural characterization and anti-UVB irradiation activity of an extracellular polysaccharide from Pantoea agglomerans.

    abstract：:Extracellular polysaccharide (EPS) produced by Pantoea agglomerans was purified for structural analysis and anti-UVB activity evaluation. The results showed that the EPS comprised of mannose and glucose in a ratio of 3:2. The molecular weight of EPS was estimated as 679 KDa. Fourier transform infrared spectrometry (FT...

    journal_title：International journal of biological macromolecules

    pub_type： 杂志文章

    doi：10.1016/j.ijbiomac.2019.06.191

    authors： Li L,Huang T,Liu H,Zang J,Wang P,Jiang X

    更新日期：2019-09-15 00:00:00

  • Isolation and characterization of Populus xyloglucan endotransglycosylase/hydrolase (XTH) involved in osmotic stress responses.

    abstract：:Xyloglucan endotransglycosylase/hydrolase (XTH) belongs to the GH16 subfamily of the glycoside hydrolases of carbohydrate active enzymes and plays an important role in the structure and function of plant cell walls. In this study, 11 members of the XTH gene family were cloned from Populus tomentosa. A bioinformatics a...

    journal_title：International journal of biological macromolecules

    pub_type： 杂志文章

    doi：10.1016/j.ijbiomac.2019.11.099

    authors： Jiang Y,Li Y,Lu C,Tang Y,Jiang X,Gai Y

    更新日期：2020-07-15 00:00:00

  • Amylopectin-g-poly(methylacrylate-co-sodium acrylate): An efficient Cd(II) binder.

    abstract：:Synthesis, characterization and Cd(II) adsorption studies of a novel biodegradable graft copolymer based on partially hydrolysed polymethylacrylate (PMA) grafted amylopectin was reported, which was prepared by first grafting of PMA chains onto the amylopectin backbone followed by partial alkaline hydrolysis. The hydro...

    journal_title：International journal of biological macromolecules

    pub_type： 杂志文章

    doi：10.1016/j.ijbiomac.2016.06.022

    authors： Sasmal D,Kolya H,Tripathy T

    更新日期：2016-10-01 00:00:00

  • Tough chitosan hydrogel based on purified regeneration and alkaline solvent as biomaterials for tissue engineering applications.

    abstract：:The chitosan based on purified regeneration could be dissolved in 6wt% aqueous NaOH without freeze-thawing cycles and acetylation processing, and such a solution system was effective and different from other dissolving methods Upon heating, a tough hydrogel was constructed from the chitosan (purified regeneration) alk...

    journal_title：International journal of biological macromolecules

    pub_type： 杂志文章

    doi：10.1016/j.ijbiomac.2017.06.017

    authors： Bi S,Bao Z,Bai X,Hu S,Cheng X,Chen X

    更新日期：2017-11-01 00:00:00

  • Bioconversion of crude glycerol to polyhydroxyalkanoate by Bacillus thuringiensis under non-limiting nitrogen conditions.

    abstract：:Glycerol has emerged as a cheap waste material due to blooming biodiesel manufacturing units worldwide. The need is to exploit the crude glycerol (CG) to produce useful products such as polyhydroxyalkanoate (PHA). Bacillus thuringiensis EGU45 was found to produce 1.5-3.5 gP HA L(-1) from feed containing 1-10% CG (vv(-...

    journal_title：International journal of biological macromolecules

    pub_type： 杂志文章

    doi：10.1016/j.ijbiomac.2015.03.046

    authors： Kumar P,Ray S,Patel SK,Lee JK,Kalia VC

    更新日期：2015-01-01 00:00:00

  • Protein function analysis of germinated Moringa oleifera seeds, and purification and characterization of their milk-clotting peptidase.

    abstract：:The present study aimed to investigate the biological functions of germinated M. oleifera seed proteins and to identify the identity of milk-clotting proteases. A total of 963 proteins were identified, and those with molecular weights between 10 and 30 kDa were most abundant. The identified proteins were mainly involv...

    journal_title：International journal of biological macromolecules

    pub_type： 杂志文章

    doi：10.1016/j.ijbiomac.2021.01.026

    authors： Wang X,He L,Zhao Q,Chen H,Shi Y,Fan J,Chen Y,Huang A

    更新日期：2021-01-09 00:00:00

  • Scaffolds containing chitosan, gelatin and graphene oxide for bone tissue regeneration in vitro and in vivo.

    abstract：:Critical-sized bone defects are augmented with cell free and cell loaded constructs to bridge bone defects. Improving the properties of three-dimensional scaffolds with multiple polymers and others is of growing interest in recent decades. Chitosan (CS), a natural biopolymer has limitations for its use in bone regener...

    journal_title：International journal of biological macromolecules

    pub_type： 杂志文章

    doi：10.1016/j.ijbiomac.2017.01.034

    authors： Saravanan S,Chawla A,Vairamani M,Sastry TP,Subramanian KS,Selvamurugan N

    更新日期：2017-11-01 00:00:00

  • Molecular crowding prevents the aggregation of protein-dextran conjugate by inducing structural changes, improves its functional properties, and stabilizes it in nanoemulsions.

    abstract：:In most of the recent research on emulsions related to food products containing protein-polysaccharide interactions established via the Maillard reaction have been used as emulsifiers. Key challenges in such studies include long reaction times, uncontrollable extent of reaction, and protein denaturation and aggregatio...

    journal_title：International journal of biological macromolecules

    pub_type： 杂志文章

    doi：10.1016/j.ijbiomac.2020.09.007

    authors： Hu M,Liu G,Du X,Zhang X,Qi B,Li Y

    更新日期：2020-12-01 00:00:00

  • Green synthesis of biopolymer-silver nanoparticle nanocomposite: an optical sensor for ammonia detection.

    abstract：:Biopolymer used for the production of nanoparticles (NPs) has attracted increasing attention. In the presence article we use aqueous solution of polysaccharide Cyamopsis tetragonaloba commonly known as guar gum (GG), from plants. GG acts as reductive preparation of silver nanoparticles which are found to be <10 nm in ...

    journal_title：International journal of biological macromolecules

    pub_type： 杂志文章

    doi：10.1016/j.ijbiomac.2012.06.033

    authors： Pandey S,Goswami GK,Nanda KK

    更新日期：2012-11-01 00:00:00

  • Biological interaction of newly synthesized astaxanthin-s-allyl cysteine biconjugate with Saccharomyces cerevisiae and mammalian α-glucosidase: In vitro kinetics and in silico docking analysis.

    abstract：:In humans, alpha-glucosidase activity is present in sucrase-isomaltase (SI) and maltase-glucoamylase (MGAM). α-glucosidase is involved in the hydrolyses of disaccharide into monosaccharides and results in hyperglycemia. Subsequently chronic hyperglycemia induces oxidative stress and ultimately leads to the secondary c...

    journal_title：International journal of biological macromolecules

    pub_type： 杂志文章

    doi：10.1016/j.ijbiomac.2018.06.027

    authors： Sakayanathan P,Loganathan C,Iruthayaraj A,Periyasamy P,Poomani K,Periasamy V,Thayumanavan P

    更新日期：2018-10-15 00:00:00

  • Enantiomeric separation of pharmaceutically important drug intermediates using a Metagenomic lipase and optimization of its large scale production.

    abstract：:In the present study, efficient enzymatic methods were developed using a recombinant metagenomic lipase (LipR1) for the synthesis of corresponding esters by the transesterification of five different pharmaceutically important secondary alcohols. The recombinant lipase (specific activity=87m6U/mg) showed maximum conver...

    journal_title：International journal of biological macromolecules

    pub_type： 杂志文章

    doi：10.1016/j.ijbiomac.2016.10.088

    authors： Kumar R,Banoth L,Banerjee UC,Kaur J

    更新日期：2017-02-01 00:00:00

  • Biophysical insight into structure-function relation of Allium sativum Protease Inhibitor by thermal, chemical and pH-induced modulation using comprehensive spectroscopic analysis.

    abstract：:In this study, we have analyzed the structural and functional changes in the nature of Allium sativum Protease Inhibitor (ASPI) on undergoing various denaturation with variable range of pH, temperature and urea (at pH 8.2). ASPI being anti-tryptic in nature has native molecular mass of ∼15kDa. The conformational stabi...

    journal_title：International journal of biological macromolecules

    pub_type： 杂志文章

    doi：10.1016/j.ijbiomac.2017.05.075

    authors： Shamsi TN,Parveen R,Naz H,Haque MA,Fatima S

    更新日期：2017-10-01 00:00:00

  • Can green solvents be alternatives for thermal stabilization of collagen?

    abstract：:"Go Green" campaign is gaining light for various industrial applications where water consumption needs to be reduced. To resolve this, industries have adopted usage of green, organic solvents, as an alternative to water. For leather making, tanning industry consumes gallons of water. Therefore, for adopting green solv...

    journal_title：International journal of biological macromolecules

    pub_type： 杂志文章

    doi：10.1016/j.ijbiomac.2014.05.021

    authors： Mehta A,Rao JR,Fathima NN

    更新日期：2014-08-01 00:00:00

  • Capability of starch derivative containing azo and carboxylic groups to tune photo-behaviors via LbL-assembly.

    abstract：:Starch-based polyelectrolyte that contained azo and carboxyl groups was prepared and applied to performed layer-by-layer (LbL) assembly with amino starch. The structure of the starch-based polyelectrolyte was characterized with UV-visible spectroscopy and 1HNMR. UV-visible spectroscopy was also utilized to confirm the...

    journal_title：International journal of biological macromolecules

    pub_type： 杂志文章

    doi：10.1016/j.ijbiomac.2019.03.106

    authors： Liu J,Xiao C

    更新日期：2019-06-15 00:00:00

  • Nitric oxide-releasing chitosan film for enhanced antibacterial and in vivo wound-healing efficacy.

    abstract：:Nitric oxide (NO) is a promising therapeutic agent with antibacterial and wound-healing properties. However, the gaseous state and short half-life of NO necessitate a formulation that can control its storage and release. In this study, we developed NO-releasing films (CS/NO film) composed of chitosan (CS) and S-nitros...

    journal_title：International journal of biological macromolecules

    pub_type： 杂志文章

    doi：10.1016/j.ijbiomac.2015.04.073

    authors： Kim JO,Noh JK,Thapa RK,Hasan N,Choi M,Kim JH,Lee JH,Ku SK,Yoo JW

    更新日期：2015-08-01 00:00:00

  • Surface charge-based rational design of aspartase modifies the optimal pH for efficient β-aminobutyric acid production.

    abstract：:β-Aminobutyric acid (BABA) can be widely used in the preparation of anti-tumor drugs, AIDS drugs, penicillin antibiotics, and plant initiators. However, the efficient, economical, and environmentally friendly production of BABA still faces challenges. Its important production enzyme, aspartase, catalyzes the substrate...

    journal_title：International journal of biological macromolecules

    pub_type： 杂志文章

    doi：10.1016/j.ijbiomac.2020.08.229

    authors： Wang Y,Xu M,Yang T,Zhang X,Rao Z

    更新日期：2020-12-01 00:00:00

  • The oxidation of potato starch by Electro-Fenton system in the presence of Fe(II) ions.

    abstract：:An efficient method was developed to prepare oxidized potato starch under the pasting point by Electro-Fenton system, in which a complex of sodium citrate and Fe2+ (SC-Fe2+) was used as catalyst, and H2O2 was used as oxidant. In the preparation process the oxidation degree of starch was controlled by the molar ratios ...

    journal_title：International journal of biological macromolecules

    pub_type： 杂志文章

    doi：10.1016/j.ijbiomac.2018.10.012

    authors： Dang X,Chen H,Shan Z,Zhen W,Yang M

    更新日期：2019-01-01 00:00:00