当前位置： SCI文献检索 > INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES期刊下所有文献 > High yield production of levan-type fructans by Gluconobacter japonicus LMG 1417.

High yield production of levan-type fructans by Gluconobacter japonicus LMG 1417.

Abstract：

:Levan, a β-2,6-glycosidic linked fructan, is a promising alternative for the inulin dominated fructan market. Although levan is already used in some cosmetic products, the commercial availability of the fructan is still limited. Here we show that Gluconobacter japonicus LMG 1417 is a potent levan-forming organism and a promising platform for the industrial production of levan. The levansucrase LevS1417, which is produced by G. japonicus LMG 1417 and secreted by a signal-peptide-independent pathway, exhibited extraordinary high activity (4726 ± 821 U mg-1 at 50 °C). A cell-free levan production based on the supernatant of the investigated strain led to a final levan yield of 157.9 ± 7.6 g L-1. The amount of secreted levansucrase was more than doubled by plasmid-mediated homologous overproduction of LevS1417 in G. japonicus LMG 1417. Accordingly, the space-time yield of cell-free levan production was doubled using the plasmid-bearing mutant.

journal_name

Int J Biol Macromol

journal_title

International journal of biological macromolecules

authors

Hövels M,Kosciow K,Kniewel J,Jakob F,Deppenmeier U

doi

10.1016/j.ijbiomac.2020.07.105

subject

Has Abstract

pub_date

2020-12-01 00:00:00

pages

295-303

eissn

0141-8130

issn

1879-0003

pii

S0141-8130(20)33858-7

journal_volume

164

pub_type

杂志文章

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