Abstract：
:Interaction between split RNA aptamer and the clinically important target, HIV-1 Tat was investigated on a biosensing surface transduced by functionally choreographed multiwall carbon nanotubes (MWCNTs). Acid oxidation was performed to functionalize MWCNTs with carboxyl functional groups. X-ray photoelectron spectroscopy analysis had profound ~2.91% increment in overall oxygen group and ~1% increment was noticed with a specific carboxyl content owing to CO and OCO bonding. The interaction between split RNA aptamer and HIV-1 Tat protein was quantified by electrical measurements with the current signal (Ids) over a gate voltage (Vgs). Initially, 34.4 mV gate voltage shift was observed by the immobilization of aptamer on MWCNT. With aptamer and HIV-1 Tat interaction, the current flow was decreased with the concomitant gate voltage shift of 23.5 mV. The attainment of sensitivity with split aptamer and HIV-1 Tat interaction on the fabricated device was 600 pM. To ensure the genuine interaction of aptamer with HIV-1 Tat, other HIV-1 proteins, Nef and p24 were interacted with aptamer and they displayed the negligible interferences with gate voltage shift of 3.5 mV and 5.7 mV, which shows 4 and 2.5 folds lesser than HIV-1 Tat interaction, respectively.
journal_name
Int J Biol Macromoljournal_title
International journal of biological macromoleculesauthors
Fatin MF,Rahim Ruslinda A,Gopinath SCB,Arshad MKMdoi
10.1016/j.ijbiomac.2018.12.066subject
Has Abstractpub_date
2019-03-15 00:00:00pages
414-422eissn
0141-8130issn
1879-0003pii
S0141-8130(18)34249-1journal_volume
125pub_type
杂志文章abstract：:As a kind of biomass that exists widely in plants, lignin shows much diversity as a functional material. In order to improve the adsorption ability, lignin was chemically modified by 5-sulfosalicylic acid and then used to adsorb methylene blue (MB) and Pb2+ from aqueous solutions. The results showed that the 5-sulfosa...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2018.10.213
更新日期：2019-02-15 00:00:00
abstract：:Superhydrophobic cotton fabrics were fabricated using chitosan/titanium dioxide (TiO2) nanocomposites. Morphology results revealed that the fabric's surface was utterly coated by the nanoparticles leading to the formation of a highly packed nano-scale structure in the case of superhydrophobic coating. X-ray photoelect...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2020.12.220
更新日期：2021-01-06 00:00:00
abstract：:A neutral polysaccharide, SPW-2, was purified from the leaves of Sambucus adnata Wall. using water extraction and alcohol precipitation, Sevage deproteination, ion exchange chromatography and gel filtration chromatography. This molecule had an average molecular weight of 7040 Da and was composed of arabinose, xylose, ...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2019.11.021
更新日期：2020-07-01 00:00:00
abstract：:The pure chitosan nanofibrous membranes with average fiber diameter of 86±18, 114±17,164±28nm were successfully prepared by electrospinning. Batch adsorption experiments of using chitosan nanofibrous membranes as adsorbent to remove acid blue-113 were conducted. The adsorption capacity of 1377mg/g was achieved by the ...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2017.08.072
更新日期：2018-01-01 00:00:00
abstract：:The interaction of the protoberberine alkaloid palmatine with single and double stranded structures of poly(A) was studied by various biophysical techniques. Comparative binding studies were also performed with double stranded DNA, t-RNA, poly(C).poly(G), poly(U) and poly(C). The results of competition dialysis, fluor...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2006.03.026
更新日期：2006-11-15 00:00:00
abstract：:A hyperbranched polysaccharide, coded as TM3a, was extracted from the Pleurotus tuber-regium sclerotia. TM3a was hybridized with xanthan gum (XG) by chemical crosslinking using sodium trimetaphosphate (STMP) to obtain new hydrogels with self-healing and release-controlled properties. The oscillatory rheological measur...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2018.09.008
更新日期：2018-12-01 00:00:00
abstract：:In the present study, we have fabricated a novel chitosan based nanocomposite (g-C3N4/Ag3PO4/CS) containing g-C3N4 and Ag3PO4 nanoparticles. The fabricated nanocomposite was characterized successfully and used as a catalyst for photocatalytic degradation of ciprofloxacin (CIP) under visible light. The TEM results reve...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2020.08.133
更新日期：2020-12-01 00:00:00
abstract：:To evaluate the structural influence of the DNA phosphate backbone on the activity of Escherichia coli DNA topoisomerase I, modified forms of oligonucleotide dA(7) were synthesized with a chiral phosphorothioate replacing the non-bridging oxygens at each position along the backbone. A deoxy-iodo-uracil replaced the 5'...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/s0141-8130(01)00165-9
更新日期：2001-10-22 00:00:00
abstract：:Wound dressing is a very important factor in the process of wound healing as proper wound care can accelerate the recovery of the wound. In this study, zein nanofibrous mats with fiber diameters around 350-500 nm were prepared by electrospinning and silver (Ag) nanoparticles around 20 nm were concurrently synthesized ...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2015.06.026
更新日期：2015-09-01 00:00:00
abstract：:The antibacterial carboxymethyl chitosan/ZnO nanocomposite hydrogels were successfully prepared via in situ formation of ZnO nanorods in the crosslinked carboxymethyl chitosan (CMCh) matrix, by treating the CMCh hydrogel matrix with zinc nitrate solution followed by the oxidation of zinc ions with alkaline solution. T...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2016.03.044
更新日期：2016-07-01 00:00:00
abstract：:Lactoglobulin is a globular milk protein for which physiological function has not been clarified. Due to its binding properties lactoglobulin might serve as a carrier for bioactive molecules. Binding of 12-, 14-, 16- and 18-carbon saturated fatty acids to bovine β-lactoglobulin has been characterised by isothermal tit...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2012.03.002
更新日期：2012-05-01 00:00:00
abstract：:The contribution of chitosan/nanocellulose (CS-NC) to the enzymatic activity of Candida rugosa lipase covalently bound on the surface of CS-NC (CRL/CS-NC) was investigated. Cellulosic material from oil palm frond leaves (OPFL) were bleached, alkaline treated and acid hydrolyzed to obtain the purified NC and used as na...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2018.03.095
更新日期：2018-07-15 00:00:00
abstract：:The in vitro digestive and fermentation properties of Sargassum pallidum polysaccharide (SPP) after ultrasound degradation were investigated. The results showed that SPP and its degraded fractions were not affected by human saliva, but slightly degraded by breaking glycosidic bonds under simulated gastrointestinal dig...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2020.07.198
更新日期：2020-12-01 00:00:00
abstract：:A crosslinked chitosan-glyoxal/TiO2 nanocomposite (CCG/TNC) was synthesized by loading different ratios of TiO2 nanoparticles into polymeric matrix of crosslinked chitosan-glyoxal (CCG) to be a promising biosorbent for methyl orange (MO). Box-Behnken design (BBD) in response surface methodology (RSM) was applied to op...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2019.02.025
更新日期：2019-05-15 00:00:00
abstract：:In this study, ginger polysaccharide (GP) was obtained from ginger by enzymatic method, its chemical properties and antitumor activity were investigated. The results indicated that the composition and proportion of GP were l‑rhamnose, d‑arabinose, d‑mannose, d‑glucose and d‑galactose in a molar ratio of 3.64:5.37:3.04...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2018.10.169
更新日期：2019-02-15 00:00:00
abstract：:A sulfated Chinese yam polysaccharide (S-CYP) was obtained from non-starch Chinese yam polysaccharide (CYP) by chlorosulfonic acid-pyridine (CSA-Pyr) method. The physicochemical characteristics and immunological activity of S-CYP were evaluated in this study. Results showed that sulfated modification changed the physi...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2020.09.213
更新日期：2020-12-15 00:00:00
abstract：:Ultrafine fibers were successfully fabricated from Channel catfish skin-extracted gelatin via electrospinning (ES). Important ES parameters, such as concentration of aqueous acid and fish gelatin solutions, and electric field intensity were examined to investigate the effects on the morphology of the gelatin nanofiber...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2010.06.002
更新日期：2010-10-01 00:00:00
abstract：:Calorimetry was used to measure the rates of irreversible denaturation of collagen in intact mammalian lens capsules, isothermally stored in water at different temperatures, and the kinetic characteristics of the process determined. At 47.4 degrees C, the number of native collagen molecules declined by a factor of 10 ...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/0141-8130(93)90025-h
更新日期：1993-10-01 00:00:00
abstract：:Twelve therapeutic mAbs, comprising 10 IgG1s and 2 IgG4s, were analyzed by a peptide mapping technique to detect and quantify C-terminal modifications. C-terminal amidated structures were found in 8 out of the 12 mAbs. An in vitro study using a commercially available peptidylglycine alpha-amidating monooxygenase (PAM)...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2012.09.016
更新日期：2013-01-01 00:00:00
abstract：:Normal corn starch was treated with acidic ethanol solution with various concentrations of ethanol (0, 10, 30, 50 and 95%) and acid (0.0026, 0.0053 and 0.0079M) and then subjected to a dry heating treatment for 1, 2 and 4h at 130°C to prepare starch nanoparticles. Size of nanoparticles was determined using DLS (dynami...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2018.11.072
更新日期：2019-02-15 00:00:00
abstract：:In this study, the rheological properties and gelation mechanism of high acyl gellan (HAG) were investigated. The critical overlap concentration (C⁎) of HAG was determined to be 0.045%, and the specific viscosity (ηsp) of HAG solutions linearly varied as the concentration of C1.42 and C3.51 before and after the concen...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2019.03.137
更新日期：2019-06-15 00:00:00
abstract：:Oxalic acid, an effective metal-chelating ligand, is abundant in natural resources. In this study, a chitosan-oxalate complex biosorbent (COCB) was prepared by an iontropic cross-linking method. The COCB beads were characterized by Fourier transform infrared (FT-IR) spectra, X-ray diffraction (XRD) and thermogravimetr...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2014.08.006
更新日期：2015-01-01 00:00:00
abstract：:The origin and some consequences of the presence of aggregates and impurities (deriving from the fermentation procedure) on the solution properties of welan gum have been studied. For this purpose, a method of purification was developed for a fermentation welan broth. It was verified that this method is ineffective fo...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/0141-8130(94)90030-2
更新日期：1994-10-01 00:00:00
abstract：:A response surface methodology was used to optimize the parameters for extracting the polysaccharides from tobacco waste (TWPs) using hot water. The extraction process, carried out under the following optimized parameters: an extraction temperature of 90°C, a ratio of water to raw material of 54, and an extraction tim...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2016.05.069
更新日期：2016-10-01 00:00:00
abstract：:Thermo-sensitive poly(N-isopropyl acrylamide-co-vinyl pyrrolidone)/chitosan [P(NIPAM-co-NVP)/CS] semi-IPN hydrogels with improved loading capacity and sustained release for anionic drugs NAP were prepared by free-radical polymerization. The LCST of hydrogels was adjusted to the vicinity of body temperature by introduc...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2012.02.013
更新日期：2012-05-01 00:00:00
abstract：:This study aims to investigate the effect of nitrogen source on curdlan production by Alcaligenes faecalis ATCC 31749. Curdlan production fell when excess nitrogen source was present, while biomass accumulation increased as the level of nitrogen source raised. Curdlan production and biomass accumulation were greater w...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2012.10.010
更新日期：2013-01-01 00:00:00
abstract：:Interaction of 1,3-bis(2-hydroxy-benzylidene)-urea (H2L1), 1,3-bis(2-hydroxy-3-methoxy-benzylidene)-urea (H2L2) and 1,3-bis(2-hydroxy-3-methoxy-benzylidene)-urea nickel(II) (NiL2) with calf-thymus DNA were investigated by UV-vis absorption, fluorescence emission and circular dichroism (CD) spectroscopy as well as cycl...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2015.03.016
更新日期：2015-01-01 00:00:00
abstract：:Considering the need of new lactic acid bacteria (LAB) for the production of novel biosurfactant (BS) molecules, the current study brings out a new insight on the exploration of cheese samples for BS producers and process optimization for industrial applications. In view of this, Lactobacillus plantarum 60FHE, Lactoba...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2020.12.140
更新日期：2021-02-15 00:00:00
abstract：:We investigated complex coacervation of sodium caseinate/Astragalus rahensis (A.r) as a function of pH with light scattering, spectrophotometry, and viscosity measurements. Interestingly, sodium caseinate/A.r displayed five structural transitions; pH 7.00 to pH ∼5.40: no interaction occurred, pH ∼5.40 to pH ∼4.80: ini...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2014.02.037
更新日期：2014-06-01 00:00:00
abstract：:Panax notoginseng is a widely used traditional Chinese medicine and has extensive pharmacological effects. In this work, water-soluble polysaccharides from Panax notoginseng were isolated and fractionated. One starch-like polysaccharide (PNPN) and six pectin fractions (PNPA-1A, PNPA-1B, PNPA-2A, PNPA-2B, PNPA-3A and P...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2020.03.233
更新日期：2020-07-15 00:00:00
