Abstract:
:Lactoglobulin is a globular milk protein for which physiological function has not been clarified. Due to its binding properties lactoglobulin might serve as a carrier for bioactive molecules. Binding of 12-, 14-, 16- and 18-carbon saturated fatty acids to bovine β-lactoglobulin has been characterised by isothermal titration calorimetry and X-ray crystallography as a part of systematic studies of lactoglobulin complexes with ligands of biological importance. The thermodynamic parameters have been determined for lauric, myristic and palmitic acid complexes revealing systematic decrease of enthalpic and increase of entropic component of ΔG with elongation of aliphatic chain. In all crystal structures determined with resolution 1.9-2.1Å, single fatty acid molecule was found in the β-barrel in extended conformation with individual pattern of interactions. Location of a fatty acid in the binding site depends on the length of aliphatic chain and influences polar interactions between protein and ligand. Systematic changes of entropic component indicate important role of water in binding process.
journal_name
Int J Biol Macromoljournal_title
International journal of biological macromoleculesauthors
Loch JI,Polit A,Bonarek P,Olszewska D,Kurpiewska K,Dziedzicka-Wasylewska M,Lewiński Kdoi
10.1016/j.ijbiomac.2012.03.002subject
Has Abstractpub_date
2012-05-01 00:00:00pages
1095-102issue
4eissn
0141-8130issn
1879-0003pii
S0141-8130(12)00099-2journal_volume
50pub_type
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