当前位置： SCI文献检索 > INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES期刊下所有文献 > Cloning, expression and characterization of β- and γ‑carbonic anhydrase from Bacillus sp. SS105 for biomimetic sequestration of CO2.

Cloning, expression and characterization of β- and γ‑carbonic anhydrase from Bacillus sp. SS105 for biomimetic sequestration of CO2.

Abstract：

:Bacterium Bacillus sp. SS105, isolated from Free Air CO2 Enriched (FACE) soil was previously screened for carbonic anhydrase activity and CO2 sequestration. In this study, strain was selected to amplify carbonic anhydrase encoding genes. The CA genes from Bacillus sp. SS105 were found to be homologous with beta‑carbonic anhydrase (β-CA) and gamma‑carbonic anhydrase (γ-CA). Both types of CA genes was cloned in pET30b (+) and expressed in E coliBL21 (DE3) with His-tag at the N-terminus. The recombinant proteins were purified by Ni-NTA affinity chromatography. The molecular size of β-CA and γ-CA were approximately 27 kDa and 25 kDa respectively. The optimum pH and temperature were found to be 8.0 and 37 °C respectively. The Zn+ was enhancing the CAs enzyme activity. Anions and modulators showed inhibitory effect on CAs at specific concentration. Functional domain analysis of both CA proteins showed conserved region of respective proteins. Recombinant enzymes were used for bio-mineralization based conversion of atmospheric CO2 into valuable calcite. Calcite formation was evaluated with or without use of enzymes and confirmed by SEM and XRD analysis. SEM result confirmed the conversion of flower-shaped unstable form of vaterite to hexagonal cubic stable form of calcite in presence of enzymes.

journal_name

Int J Biol Macromol

journal_title

International journal of biological macromolecules

authors

Maheshwari N,Kumar M,Thakur IS,Srivastava S

doi

10.1016/j.ijbiomac.2019.03.082

subject

Has Abstract

pub_date

2019-06-15 00:00:00

pages

445-452

eissn

0141-8130

issn

1879-0003

pii

S0141-8130(19)30048-0

journal_volume

131

pub_type

杂志文章

相关文献

INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES文献大全
  • Optimized production and characterization of a detergent-stable protease from Lysinibacillus fusiformis C250R.

    abstract：:In this study, we aimed to optimize the cultural and nutritional conditions for protease production by Lysinibacillus fusiformis strain C250R in submerged fermentation process using statistical methodology. The most significant factors (gruel, wheat bran, yeast extract, and FeSO4) were identified by Plackett-Burman de...

    journal_title：International journal of biological macromolecules

    pub_type： 杂志文章

    doi：10.1016/j.ijbiomac.2017.03.051

    authors： Mechri S,Kriaa M,Ben Elhoul Berrouina M,Omrane Benmrad M,Zaraî Jaouadi N,Rekik H,Bouacem K,Bouanane-Darenfed A,Chebbi A,Sayadi S,Chamkha M,Bejar S,Jaouadi B

    更新日期：2017-08-01 00:00:00

  • Regulation of the equilibrium between G-quadruplex and duplex DNA in promoter of human c-myc oncogene by a pyrene derivative.

    abstract：:It has been established that the equilibrium between duplex and G-quadruplex of the nuclease hypersensitivity element III1 (NHE III1) in human c-myc promoter is linked with this gene's transcription. Using NMR and ESI-MS, we have found a pyrene derivative, DMAPP, is able to modulate this equilibrium and, thus, might h...

    journal_title：International journal of biological macromolecules

    pub_type： 杂志文章

    doi：10.1016/j.ijbiomac.2011.05.024

    authors： Zhang Z,He X,Yuan G

    更新日期：2011-12-01 00:00:00

  • Effects of the ultra-high pressure on structure and α-glucosidase inhibition of polysaccharide from Astragalus.

    abstract：:A novel homogeneous polysaccharide fraction (APS) was extracted from Astragalus by hot water and purified by Sephadex G-100 and G-75 column. Its molecular weight was 693kDa. APS and APS with ultra-high pressure treatment exhibited significant inhibitory abilities on a-glucosidase, inhibition rate from high to low in o...

    journal_title：International journal of biological macromolecules

    pub_type： 杂志文章

    doi：10.1016/j.ijbiomac.2016.03.024

    authors： Zhu ZY,Luo Y,Dong GL,Ren YY,Chen LJ,Guo MZ,Wang XT,Yang XY,Zhang Y

    更新日期：2016-06-01 00:00:00

  • Calcium-dependent conformational transition of calmodulin determined by Fourier transform infrared spectroscopy.

    abstract：:The Ca(2+)-induced conformational changes in calmodulin (CaM) were monitored by Fourier transform infrared spectroscopy (FT-IR) at different molar ratios of Ca(2+) to CaM. The results show that these changes occur in two distinctive transitions. The first transition involves significant changes in the overall secondar...

    journal_title：International journal of biological macromolecules

    pub_type： 杂志文章

    doi：10.1016/j.ijbiomac.2013.02.004

    authors： Yu T,Wu G,Yang H,Wang J,Yu S

    更新日期：2013-05-01 00:00:00

  • Preparation and antioxidant properties of low molecular holothurian glycosaminoglycans by H2O2/ascorbic acid degradation.

    abstract：:An effective method of free radical degradation induced by H2O2/ascorbic acid, which was different from previously reported H2O2/Cu2+ method, was developed to depolymerize a novel glycosaminoglycan from Holothuria mexicana (HmG). The results indicated that the degradation efficiency increased with the reduced solution...

    journal_title：International journal of biological macromolecules

    pub_type： 杂志文章

    doi：10.1016/j.ijbiomac.2017.10.161

    authors： Mou J,Wang C,Li Q,Qi X,Yang J

    更新日期：2018-02-01 00:00:00

  • Adsorption of mercury ions from wastewater aqueous solution by amide functionalized cellulose from sugarcane bagasse.

    abstract：:A novel effective cellulose-based adsorbent was prepared through two common reactions, which included the esterification of sugarcane bagasse cellulose with excess stearic acid and the reaction of grafting polyacrylamide brush by ultraviolet radiation initiation. The adsorbent can effectively adsorb Hg(II) ion from wa...

    journal_title：International journal of biological macromolecules

    pub_type： 杂志文章

    doi：10.1016/j.ijbiomac.2017.11.027

    authors： Sun N,Wen X,Yan C

    更新日期：2018-03-01 00:00:00

  • Aqueous extraction of pectin from sour orange peel and its preliminary physicochemical properties.

    abstract：:Sour orange peel, a by-product of the fruit juice industry, was used as a source of pectin. The effects of temperature (75-95°C), time (30-90 min), and liquid-solid ratio (20-40, v/w) were investigated on yield, methoxylation degree (DE), and galacturonic acid content using a Box-Behnken design and response surface me...

    journal_title：International journal of biological macromolecules

    pub_type： 杂志文章

    doi：10.1016/j.ijbiomac.2015.11.007

    authors： Hosseini SS,Khodaiyan F,Yarmand MS

    更新日期：2016-01-01 00:00:00

  • A comparative study of the suitability of different cereal straws for lignocellulose nanofibers isolation.

    abstract：:The aim of this work is to study the suitability of different soda cereal straw pulps (wheat, barley, corn and oats) to produce lignocellulose nanofibers (LCNF). For the study of the influence of the composition and physical characteristics, the pulps were characterized, observing that the physical characteristics do ...

    journal_title：International journal of biological macromolecules

    pub_type： 杂志文章

    doi：10.1016/j.ijbiomac.2017.05.156

    authors： Espinosa E,Sánchez R,Otero R,Domínguez-Robles J,Rodríguez A

    更新日期：2017-10-01 00:00:00

  • Structural characterization and neuroprotective effect of a polysaccharide from Corydalis yanhusuo.

    abstract：:In the present study, we investigated the neuroprotective effects of a purified Corydalis yanhusuo polysaccharide (CYP) on Aβ (25-35)-induced neurotoxicity and explored its underlying molecular mechanisms in PC12 cells. The results showed pretreatment with CYP (25, 50, and 100 μg/ml) prior to Aβ (25-35) exposure signi...

    journal_title：International journal of biological macromolecules

    pub_type： 杂志文章

    doi：10.1016/j.ijbiomac.2020.01.180

    authors： He Y,Xu W,Qin Y

    更新日期：2020-08-15 00:00:00

  • Association of hypoxia inducible factor-1 alpha exon 12 mutation in diabetic patients with and without diabetic foot ulcer.

    abstract：:Hypoxia inducible factor 1 alpha (HIF-1α) is a key regulator of the genes involved in the cellular response to hypoxia. This study aims to determine the HIF-1α gene polymorphism and its association with protein expression in diabetic subjects with and without diabetic foot ulcers (DFU). A total of 529 patients with T2...

    journal_title：International journal of biological macromolecules

    pub_type： 杂志文章

    doi：10.1016/j.ijbiomac.2018.08.011

    authors： Pichu S,Vimalraj S,Sathiyamoorthy J,Viswanathan V

    更新日期：2018-11-01 00:00:00

  • Controlled release of drug and better bioavailability using poly(lactic acid-co-glycolic acid) nanoparticles.

    abstract：:Tamoxifen (Tmx) embedded poly(lactic-co-glycolic acid) (PLGA) nanoparticles (PLGA-Tmx) is prepared to evaluate its better DNA cleavage potential, cytotoxicity using Dalton's lymphoma ascite (DLA) cells and MDA-MB231 breast cancer cells. PLGA-Tmx nanoparticles are prepared through emulsified nanoprecipitation technique...

    journal_title：International journal of biological macromolecules

    pub_type： 杂志文章

    doi：10.1016/j.ijbiomac.2016.04.065

    authors： Pandey SK,Patel DK,Maurya AK,Thakur R,Mishra DP,Vinayak M,Haldar C,Maiti P

    更新日期：2016-08-01 00:00:00

  • An immune-responsive PGRP-S1 regulates the expression of antibacterial peptide genes in diamondback moth, Plutella xylostella (L.).

    abstract：:Peptidoglycan recognition proteins (PGRPs) are family of pattern recognition receptors (PRRs) and triggers the innate immune system (IIS) against the microbial infection. Although PGRPs have been intensively studied in model insects, they remain uncharacterized in most of the non-model insects. Here, we cloned and cha...

    journal_title：International journal of biological macromolecules

    pub_type： 杂志文章

    doi：10.1016/j.ijbiomac.2019.09.081

    authors： Zhang Z,Kong J,De Mandal S,Li S,Zheng Z,Jin F,Xu X

    更新日期：2020-01-01 00:00:00

  • A novel glycosaminoglycan-like polysaccharide from abalone Haliotis discus hannai Ino: purification, structure identification and anticoagulant activity.

    abstract：:A novel glycosaminoglycan-like sulfated polysaccharide (AAP) from the pleopods of Haliotis discus hannai Ino was purified by DEAE ion exchange chromatography followed with S-300 HR geltrion chromatography. Chemical composition analysis indicated that AAP was composed of galactosamine, glucuronic acid, fucose, galactos...

    journal_title：International journal of biological macromolecules

    pub_type： 杂志文章

    doi：10.1016/j.ijbiomac.2011.09.017

    authors： Li G,Chen S,Wang Y,Xue Y,Chang Y,Li Z,Wang J,Xue C

    更新日期：2011-12-01 00:00:00

  • Polyethyleneimine-protein interactions and implications on protein stability.

    abstract：:Protein stability assessment of seven model proteins in the presence of low molecular weight polyethyleneimine (PEI, MW 2000 Da) was performed. Thermodynamic stability, monitored by circular dichroism (CD) spectroscopy, showed that the polymer did not have a major effect on the melting temperature (T(m)) of the basic ...

    journal_title：International journal of biological macromolecules

    pub_type： 杂志文章

    doi：10.1016/j.ijbiomac.2010.04.003

    authors： Mazzaferro L,Breccia JD,Andersson MM,Hitzmann B,Hatti-Kaul R

    更新日期：2010-07-01 00:00:00

  • Phosphorylated Codonopsis pilosula polysaccharide could inhibit the virulence of duck hepatitis A virus compared with Codonopsis pilosula polysaccharide.

    abstract：:To screen effective anti-duck hepatitis A virus (DHAV) drugs, we applied STMP-STPP method to prepare phosphorylated Codonopsis pilosula polysaccharide (pCPPS), the phosphorylation-modified product of Codonopsis pilosula polysaccharide (CPPS). The IR spectrum and field emission scanning electron microscope (FE-SEM) wer...

    journal_title：International journal of biological macromolecules

    pub_type： 杂志文章

    doi：10.1016/j.ijbiomac.2016.10.002

    authors： Ming K,Chen Y,Yao F,Shi J,Yang J,Du H,Wang X,Wang Y,Liu J

    更新日期：2017-01-01 00:00:00

  • It takes two to tango - The case of thebaine 6-O-demethylase.

    abstract：:Thebaine 6-O-demethylase (T6ODM) is an Fe(II)/2-oxoglutarate-dependent dioxygenase catalysing two oxidative O-demethylation reactions in morphine biosynthesis. Its crystal structure revealed a large active site pocket which is at least two times larger than necessary to accommodate a substrate (thebaine or oripavine) ...

    journal_title：International journal of biological macromolecules

    pub_type： 杂志文章

    doi：10.1016/j.ijbiomac.2020.07.030

    authors： Kachhap S,Wojdyla Z,Komorek P,Kluza A,Kurpiewska K,Jachimska B,Borowski T

    更新日期：2020-11-15 00:00:00

  • Simultaneous adsorption of Cu2+ and Acid fuchsin (AF) from aqueous solutions by CMC/bentonite composite.

    abstract：:Carboxymethyl-chitosan (CMC)/bentonite composite was prepared by the method of membrane-forming, and characterized by Fourier transform infrared spectroscopy (FTIR) and X-ray diffraction (XRD) techniques. The simultaneous adsorption of Cu2+ and Acid fuchsin (AF) applying CMC/bentonite composite as an adsorbent in sing...

    journal_title：International journal of biological macromolecules

    pub_type： 杂志文章

    doi：10.1016/j.ijbiomac.2018.04.075

    authors： Gong N,Liu Y,Huang R

    更新日期：2018-08-01 00:00:00

  • Salt-regulated reversible fibrillation of Mycobacterium tuberculosis isocitrate lyase: Concurrent restoration of structure and activity.

    abstract：:Protein fibrillation is associated with a number of neurodegenerative diseases. Nevertheless, several proteins not related to disease can also form fibrils in vitro under specific conditions. In the present study, we demonstrate the reversible fibrillation of a globular protein that is modulated by salt under physiolo...

    journal_title：International journal of biological macromolecules

    pub_type： 杂志文章

    doi：10.1016/j.ijbiomac.2017.06.008

    authors： Shukla H,Kumar R,Sonkar A,Mitra K,Akhtar MS,Tripathi T

    更新日期：2017-11-01 00:00:00

  • Synthesis of chitosan based molecularly imprinted polymer for pipette-tip solid phase extraction of Rhodamine B from chili powder samples.

    abstract：:The purpose work is devoted to design of a simple, one-pot and green approach for the synthesis of molecularly imprinted polymer to construct a selective sorbent for pipette-tip solid phase extraction of Rhodamine B from chili powder samples and its subsequence separation and quantification by high performance liquid ...

    journal_title：International journal of biological macromolecules

    pub_type： 杂志文章

    doi：10.1016/j.ijbiomac.2019.07.196

    authors： Bagheri AR,Ghaedi M

    更新日期：2019-10-15 00:00:00

  • Microbial-based synthesis of highly elastomeric biodegradable poly(3-hydroxybutyrate-co-4-hydroxybutyrate) thermoplastic.

    abstract：:This study reports the production of P(3HB-co-4HB) [Poly(3-hydroxybutyrate-co-4-hydroxybutyrate)] in possession of high molecular weight and elastomeric properties by Cupriavidus sp. USMAA1020 in single-stage mixed-substrate cultivation system. 1,4-butanediol and 1,6-hexanediol are found to be efficient substrate mixt...

    journal_title：International journal of biological macromolecules

    pub_type： 杂志文章

    doi：10.1016/j.ijbiomac.2017.03.179

    authors： Huong KH,Teh CH,Amirul AA

    更新日期：2017-08-01 00:00:00

  • In vitro and in vivo antioxidant activity of a fructan from the roots of Arctium lappa L.

    abstract：:To explore new antioxidant resource from food, a water-soluble polysaccharide (ALP1) was extracted and purified from the roots of Arctium lappa L. (A. lappa L.) through hot water extraction followed by ethanol precipitation, ion-exchange chromatography and gel filtration. The antioxidant activity of ALP1 was then eval...

    journal_title：International journal of biological macromolecules

    pub_type： 杂志文章

    doi：10.1016/j.ijbiomac.2014.01.062

    authors： Liu W,Wang J,Zhang Z,Xu J,Xie Z,Slavin M,Gao X

    更新日期：2014-04-01 00:00:00

  • Influence of molecular weight on structure and rheological properties of microcrystalline chitosan mixtures.

    abstract：:In the present work, the atomic force microscopy (AFM) studies and rheological properties of aqueous solutions of microcrystalline chitosan (MCCh), polyacrylamide (PAM) and their mixtures at different weight ratios have been investigated. Flow measurements were carried out using on solutions of native polymers and the...

    journal_title：International journal of biological macromolecules

    pub_type： 杂志文章

    doi：10.1016/j.ijbiomac.2015.05.026

    authors： Lewandowska K

    更新日期：2015-08-01 00:00:00

  • Antimicrobial cerium ion-chitosan crosslinked alginate biopolymer films: A novel and potential wound dressing.

    abstract：:Wound dressings require good antiseptic properties, mechanical strength and, more trustably, natural material ingredients. Antimicrobial properties of cerium ions and chitosan are known and alginate based wound dressings are commercially available. In this study, the advantages of these materials were combined and alg...

    journal_title：International journal of biological macromolecules

    pub_type： 杂志文章

    doi：10.1016/j.ijbiomac.2017.07.144

    authors： Kaygusuz H,Torlak E,Akın-Evingür G,Özen İ,von Klitzing R,Erim FB

    更新日期：2017-12-01 00:00:00

  • Characterization of pH-induced transitions of Entamoeba histolytica D-phosphoglycerate dehydrogenase.

    abstract：:Entamoeba histolytica D-phosphoglycerate dehydrogenase (EhPGDH) exists as a functionally active homodimer at pH 7. Our earlier studies have shown that ionic interactions are essentially required for the oligomeric status and activity of the protein. Present study focuses on pH associated structural modulations of EhPG...

    journal_title：International journal of biological macromolecules

    pub_type： 杂志文章

    doi：10.1016/j.ijbiomac.2015.04.058

    authors： Mishra V,Kumar A,Ali V,Zhang KY,Nozaki T

    更新日期：2015-08-01 00:00:00

  • Anti-Trop2 antibody-conjugated bioreducible nanoparticles for targeted triple negative breast cancer therapy.

    abstract：:Trop2, a transmembrane glycoprotein, has emerged as a biomarker for targeted cancer therapy since it is overexpressed in 80% of triple negative breast cancer (TNBC) patients. For the site-specific delivery of the anticancer drug into TNBC, anti-Trop2 antibody-conjugated nanoparticles (ST-NPs) were prepared as the pote...

    journal_title：International journal of biological macromolecules

    pub_type： 杂志文章

    doi：10.1016/j.ijbiomac.2017.10.113

    authors： Son S,Shin S,Rao NV,Um W,Jeon J,Ko H,Deepagan VG,Kwon S,Lee JY,Park JH

    更新日期：2018-04-15 00:00:00

  • New generation of controlled release phosphorus fertilizers based on biological macromolecules: Effect of formulation properties on phosphorus release.

    abstract：:The main objective of this work was to study the possibility of using polysaccharides and lignin as a coating material for water-soluble triple superphosphate (TSP) granular fertilizers. In this study, composites based on three polysaccharides (sodium alginate (alginic acid sodium salt): AL, kappa-carrageenan: CR and ...

    journal_title：International journal of biological macromolecules

    pub_type： 杂志文章

    doi：10.1016/j.ijbiomac.2019.12.005

    authors： Fertahi S,Bertrand I,Ilsouk M,Oukarroum A,Amjoud M,Zeroual Y,Barakat A

    更新日期：2020-01-15 00:00:00

  • Characterization of Camptotheca acuminata 10-hydroxygeraniol oxidoreductase and iridoid synthase and their application in biological preparation of nepetalactol in Escherichia coli featuring NADP+ - NADPH cofactors recycling.

    abstract：:Nepetalactol, an iridoid with four chiral carbons, is a crucial component of aphid sex pheromones that have been employed with great success to control the insect-related diseases. Despite of agricultural usage as end products, iridoids are fundamental biosynthetic intermediates for pharmaceutically important monoterp...

    journal_title：International journal of biological macromolecules

    pub_type： 杂志文章

    doi：10.1016/j.ijbiomac.2020.06.223

    authors： Awadasseid A,Li W,Liu Z,Qiao C,Pang J,Zhang G,Luo Y

    更新日期：2020-11-01 00:00:00

  • Isolation, cloning and characterization of an azoreductase and the effect of salinity on its expression in a halophilic bacterium.

    abstract：:Understanding the molecular mechanisms of azo dye decolorization is important for the development of effective bioremediation for textile-colored wastewater. A halophilic bacterium Halomonas sp. strain GT was isolated, which could degrade the azo dye Acid Brilliant Scarlet GR at 10% NaCl. The complete genome sequence ...

    journal_title：International journal of biological macromolecules

    pub_type： 杂志文章

    doi：10.1016/j.ijbiomac.2018.11.175

    authors： Tian F,Guo G,Zhang C,Yang F,Hu Z,Liu C,Wang SW

    更新日期：2019-02-15 00:00:00

  • Thyroxine-loaded chitosan/carboxymethyl cellulose/hydroxyapatite hydrogels enhance angiogenesis in in-ovo experiments.

    abstract：:Angiogenesis is one of the most important processes in repair and regeneration of many tissues and organs. Blood vessel formation also play a major role in repair of dental tissue(s) after ailments like periodontitis. Here we report the preparation of chitosan/carboxymethyl cellulose/hydroxyapatite based hydrogels, lo...

    journal_title：International journal of biological macromolecules

    pub_type： 杂志文章

    doi：10.1016/j.ijbiomac.2019.10.043

    authors： Malik MH,Shahzadi L,Batool R,Safi SZ,Khan AS,Khan AF,Chaudhry AA,Rehman IU,Yar M

    更新日期：2020-02-15 00:00:00

  • Polymer hydrogel from carboxymethyl guar gum and carbon nanotube for sustained trans-dermal release of diclofenac sodium.

    abstract：:Novel carboxymethyl guar gum (CMG)-chemically modified multiwalled carbon nanotube (MCNT) hybrid hydrogels were synthesized at different MCNT levels as potential device for sustained trans-dermal release of diclofenac sodium. Spectroscopy together with morphology, thermogravimetry, and rheological studies proved relat...

    journal_title：International journal of biological macromolecules

    pub_type： 杂志文章

    doi：10.1016/j.ijbiomac.2011.08.003

    authors： Giri A,Bhowmick M,Pal S,Bandyopadhyay A

    更新日期：2011-12-01 00:00:00