Abstract：
:Bacillus nealsonii PN-11 produces thermo-alkalistable mannanase and protease active in wide temperature and pH range. Optimization of coproduction of protease and mannanase from this strain and application of cocktail of these enzymes as detergent additives were studied. On optimization mannanase yield of 834Ug-1 (11.12 fold increase) and protease yield of 70Ug-1 (4.7 fold increase) could be obtained in a single fermentation. Purification and characterization of mannanase have been done earlier and protease was done during this study and has a molecular mass of 48kDa. pH and temperature optima for protease were 10.0 and 65°C respectively. It was completely stable at 60°C for 3h and retained >80% of activity at pH 11.0 for 1h. Both the enzymes were compatible with detergents individually and in a combination. The wash performance of the detergent on different type of stains improved when protease or mannanase were used individually. However destaining was more efficient when a combination of mannanase and protease was used.
journal_name
Int J Biol Macromoljournal_title
International journal of biological macromoleculesauthors
David A,Singh Chauhan P,Kumar A,Angural S,Kumar D,Puri N,Gupta Ndoi
10.1016/j.ijbiomac.2017.09.037subject
Has Abstractpub_date
2018-03-01 00:00:00pages
1176-1184eissn
0141-8130issn
1879-0003pii
S0141-8130(17)32746-0journal_volume
108pub_type
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