当前位置： SCI文献检索 > INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES期刊下所有文献 > Optimized purification process of polysaccharides from Carex meyeriana Kunth by macroporous resin, its characterization and immunomodulatory activity.

Optimized purification process of polysaccharides from Carex meyeriana Kunth by macroporous resin, its characterization and immunomodulatory activity.

Abstract：

:The analytical hierarchy process (AHP) combined with response surface methodology (RSM) was used to optimize the purification conditions of crude polysaccharides from Carex meyeriana Kunth (CMKP) by macroporous resin. The optimal purification conditions as follows: elution volume of 2.74 BV, flow rate of 1.88 BV/h, and sample concentration of 2.10 mg/mL. Under the optimal conditions, the purification comprehensive score was 63.59 ± 1.56% (n = 3), which was agreed closely to the predicted value (64.02%). Two fractions of polysaccharides (CMKP-1 and CMKP-2) were obtained using a DEAE-52 column. Fourier Transform infrared (FTIR) spectroscopy spectra revealed the general characteristic absorption peaks of CMKP-1 and CMKP-2. Congo-red test indicated that CMKP-1 and CMKP-2 have triple helix conformation. CMKP-1 and CMKP-2 were both composed of Man, Rib, Rha, GluA, GalA, Glu, Gal, Xyl, Ara and Fuc in a molar ratio of 8.93:1:4.62:1.41:1.78:10.7:24.69:4.51:15.92:1.27 and 7.85:1:5.05:1.99:3.15:12.47:15.41:1.54:6.91:3.15, respectively. The major molecular weight of CMKP-1 and CMKP-2 was 22.30 kDa and 48.10 kDa, respectively. Moreover, CMKP-1 and CMKP-2 had immunostimulatory effects on RAW264.7 cells in a certain concentration range. It could be used as potential immunomodulatory agents in medicine or functional food fields.

journal_name

Int J Biol Macromol

journal_title

International journal of biological macromolecules

authors

Hu Z,Zhou H,Li Y,Wu M,Yu M,Sun X

doi

10.1016/j.ijbiomac.2019.03.207

subject

Has Abstract

pub_date

2019-07-01 00:00:00

pages

76-86

eissn

0141-8130

issn

1879-0003

pii

S0141-8130(18)35826-4

journal_volume

132

pub_type

杂志文章

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